scholarly journals Molecular Regulation of Lipogenesis, Adipogenesis and Fat Deposition in Chicken

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 414
Author(s):  
Sara Nematbakhsh ◽  
Chong Pei Pei ◽  
Jinap Selamat ◽  
Noordiana Nordin ◽  
Lokman Hakim Idris ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Lipid Metabolism ◽  
Meat Quality ◽  
Regulatory Mechanism ◽  
Fat Metabolism ◽  
Fat Deposition ◽  
Molecular Regulation ◽  
Feeding Strategies ◽  
Meat Production ◽  
Transcriptional Level

In the poultry industry, excessive fat deposition is considered an undesirable factor, affecting feed efficiency, meat production cost, meat quality, and consumer’s health. Efforts to reduce fat deposition in economically important animals, such as chicken, can be made through different strategies; including genetic selection, feeding strategies, housing, and environmental strategies, as well as hormone supplementation. Recent investigations at the molecular level have revealed the significant role of the transcriptional and post-transcriptional regulatory networks and their interaction on modulating fat metabolism in chickens. At the transcriptional level, different transcription factors are known to regulate the expression of lipogenic and adipogenic genes through various signaling pathways, affecting chicken fat metabolism. Alternatively, at the post-transcriptional level, the regulatory mechanism of microRNAs (miRNAs) on lipid metabolism and deposition has added a promising dimension to understand the structural and functional regulatory mechanism of lipid metabolism in chicken. Therefore, this review focuses on the progress made in unraveling the molecular function of genes, transcription factors, and more notably significant miRNAs responsible for regulating adipogenesis, lipogenesis, and fat deposition in chicken. Moreover, a better understanding of the molecular regulation of lipid metabolism will give researchers novel insights to use functional molecular markers, such as miRNAs, for selection against excessive fat deposition to improve chicken production efficiency and meat quality.

scholarly journals A Study of the Regulatory Mechanism of the CB1/PPARγ2/PLIN1/HSL Pathway for Fat Metabolism in Cattle

2021 ◽  
Vol 12 ◽  
Author(s):  
Ruili Liu ◽  
Xianxun Liu ◽  
Xuejin Bai ◽  
Chaozhu Xiao ◽  
Yajuan Dong
Keyword(s):  
Lipid Metabolism ◽  
Regulatory Mechanism ◽  
Fatty Acid Content ◽  
Fat Metabolism ◽  
Lipid Synthesis ◽  
Fat Deposition ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Lipid Formation

Fat metabolism is closely related to the economic characteristics of beef cattle. Therefore, regulating fat deposition and increasing intramuscular fat deposition are among the main goals of breeders. In this study, we aim to explore the regulatory role of CB1 gene on PPARγ2/PLIN1/HSL pathway in fat metabolism, and to further explore the differential expression of regulatory factors of this pathway in Shandong black cattle and Luxi cattle. In this study, CB1 overexpression stimulated lipid synthesis in adipocytes to some extent by increasing the levels of FASN and ACSL1. CB1 inhibitors reduce the lipid content in adipocytes and reduce the expression of GLUT1 and Insig1. In addition, overexpression of CB1 decreased the expression of PPARγ2 and led to an increase in PLIN1 expression and a decrease in HSL expression in adipocytes. We also found that the CB1/PPARγ2/PLIN1/HSL was differentially expressed in the different breeds of cattle and was involved in the regulation of fat metabolism, which affected the fatty acid content in the longissimus dorsi muscle of the two breeds. In short, CB1 participates in lipid metabolism by regulating HSL in the PPARγ2 and PLIN1 pathways, and improves lipid formation in adipocytes. In conclusion, CB1/PPARγ2/PLIN1/HSL pathway may be involved in the regulation of lipid metabolism.

scholarly journals The Regulatory Mechanism of Water Activities on Aflatoxins Biosynthesis and Conidia Development, and Transcription Factor AtfB Is Involved in This Regulation

Toxins ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 431
Author(s):  
Longxue Ma ◽  
Xu Li ◽  
Xiaoyun Ma ◽  
Qiang Yu ◽  
Xiaohua Yu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Environmental Factors ◽  
Health Risks ◽  
Sexual Development ◽  
Regulatory Mechanism ◽  
Food Storage ◽  
Economic Losses ◽  
Transcriptional Level ◽  
Conidia Production

Peanuts are frequently infected by Aspergillus strains and then contaminated by aflatoxins (AF), which brings out economic losses and health risks. AF production is affected by diverse environmental factors, especially water activity (aw). In this study, A. flavus was inoculated into peanuts with different aw (0.90, 0.95, and 0.99). Both AFB1 yield and conidia production showed the highest level in aw 0.90 treatment. Transcriptional level analyses indicated that AF biosynthesis genes, especially the middle- and later-stage genes, were significantly up-regulated in aw 0.90 than aw 0.95 and 0.99. AtfB could be the pivotal regulator response to aw variations, and could further regulate downstream genes, especially AF biosynthesis genes. The expressions of conidia genes and relevant regulators were also more up-regulated at aw 0.90 than aw 0.95 and 0.99, suggesting that the relative lower aw could increase A. flavus conidia development. Furthermore, transcription factors involved in sexual development and nitrogen metabolism were also modulated by different aw. This research partly clarified the regulatory mechanism of aw on AF biosynthesis and A. flavus development and it would supply some advice for AF prevention in food storage.

scholarly journals Rearing pattern alters porcine myofiber type, fat deposition, associated microbial communities and functional capacity

2019 ◽  
Author(s):  
Keke Qi ◽  
Xiaoming Men ◽  
Jie Wu ◽  
Zi wei Xu
Keyword(s):  
Lipid Metabolism ◽  
Microbial Communities ◽  
Meat Quality ◽  
Relative Abundance ◽  
Fat Deposition ◽  
Metagenomic Sequencing ◽  
Free Range ◽  
Free Grazing ◽  
Rearing Pattern ◽  
Myofiber Type

Abstract Background: The Chinese believe that the meat of pigs reared in the past with free range tastes better than that of the pigs reared indoor on a large scale today. Gastrointestinal microflora is closely associated with the main factor of meat flavour, including fibre characteristics and lipid metabolism. Our method in this study involved different raising patterns within the semi free-grazing farm (FF) or indoor feeding farm (DF), the measurement of fat deposition and myofibre type by paraffin section and reverse transcription polymerase chain reaction and the identification of microbiome and functional capacities associated with meat quality through metagenomic sequencing. Results: Results showed that the fat area in muscle and adipose tissue and the myofibre density significantly increased in the pigs of the FF group. The relative abundance of bacteria associated with lipid metabolism, such as g_Oscillibacter, in the feces of the FF group was higher than that in DF group, and the relative abundance of some bacteria with probiotic function, including g_Lactobacillus and g_Clostridium, was lower than that in DF group. A significant positive correlation was found in the intramuscular fat area of g__Clostridium, whereas a significant negative correlation was observed with abdominal fat area, myofibre density and ATGL mRNA expression of health-related bacteria, such as g__Butyricicoccus, g__Eubacterium, g__Phascolarctobacterium and g__Oribacterium. KEGG analysis showed that pigs raised in free free-grazing farm can activate the pathway of inosine monophosphate (IMP) biosynthesis, glycolysis/gluconeogenesis and alanine, aspartate and glutamate metabolism. Conclusions: Free range feeding improves meat quality by changing the fibre type, myofibre density and metabolic pathways related to flavour amino acids, IMP or glycolysis/gluconeogenesis in muscle. However, prolonged feeding cycle increases fat deposition and associated microbial communities. Keywords: Rearing pattern, Myofiber type, Fat deposition, Gut micobiota

scholarly journals Overexpression of PLIN1 Promotes Lipid Metabolism in Bovine Adipocytes

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1944
Author(s):  
Shijun Li ◽  
Sayed Haidar Abbas Raza ◽  
Chunping Zhao ◽  
Gong Cheng ◽  
Linsen Zan
Keyword(s):  
Lipid Metabolism ◽  
Differentially Expressed Genes ◽  
Fat Metabolism ◽  
Vital Role ◽  
Surface Proteins ◽  
Differentially Expressed ◽  
Transcriptional Level ◽  
Proliferation And Differentiation ◽  
Ppar Signaling ◽  
Bovine Adipocytes

Perilipin 1 (PLIN1) is a protein encoded by the PLIN1 gene in eukaryotes. PLIN1 is a member of the PAT protein family, a family of proteins related to lipid droplet (LD) surface proteins. PLIN1 phosphorylation plays a vital role during fat metabolism of adipose tissue lipolysis and fat storage in adipocytes. However, to further explore the regulation of the PLIN1 gene on the proliferation, differentiation and lipid metabolism of bovine adipocytes. In this study, the mRNA expression of PLIN1, at day six, was the highest during bovine adipocyte differentiation. Moreover, PLIN1 can promote the proliferation and differentiation of preadipocytes in cattle. On the sixth day, after transfection with, and overexpression of, the PLIN1 gene in bovine preadipocytes via adenovirus, cell samples were collected, and transcriptome sequencing was performed. A total of 1923 differentially expressed genes were detected. Through GO and KEGG pathway analysis, the differentially expressed genes were established to be mainly enriched in the AMPK, Wnt, and PPAR signaling pathways related to fat proliferation and differentiation. In conclusion, at the transcriptional level, PLIN1 plays an important role in regulating fat proliferation and metabolism. Additionally, the sequencing results screened new differentially expressed genes related to fat metabolism, providing theoretical support for molecular breeding of Qinchuan beef cattle.

scholarly journals Rearing pattern alters porcine myofiber type, fat deposition, associated microbial communities and functional capacity

2019 ◽  
Author(s):  
Keke Qi ◽  
Xiaoming Men ◽  
Jie Wu ◽  
Zi wei Xu
Keyword(s):  
Lipid Metabolism ◽  
Microbial Communities ◽  
Meat Quality ◽  
Relative Abundance ◽  
Fat Deposition ◽  
Metagenomic Sequencing ◽  
Free Range ◽  
Free Grazing ◽  
Rearing Pattern ◽  
Myofiber Type

Abstract Background: The Chinese believe that the meat of pigs reared in the past with free range tastes better than that of the pigs reared indoor on a large scale today. Gastrointestinal microflora is closely associated with the main factor of meat flavour, including fibre characteristics and lipid metabolism. Our method in this study involved different raising patterns within the semi free-grazing farm (FF) or indoor feeding farm (DF), the measurement of fat deposition and myofiber type by paraffin section and reverse transcription polymerase chain reaction and the identification of microbiome and functional capacities associated with meat quality through metagenomic sequencing. Results: Results showed that the fat area in muscle and adipose tissue and the myofiber density significantly increased in the pigs of the FF group. The relative abundance of bacteria associated with lipid metabolism, such as g_Oscillibacter, in the feces of the FF group was higher than that in DF group, and the relative abundance of some bacteria with probiotic function, including g_Lactobacillus and g_Clostridium, was lower than that in DF group. The abundance of g__Clostridium was significantly positively correlated with the intramuscular fat area, whereas health-related bacteria, such as g__Butyricicoccus, g__Eubacterium, g__Phascolarctobacterium and g__Oribacterium, was significantly negatively correlated with abdominal fat area, myofiber density and adipose triglyceride lipase (ATGL) mRNA expression. KEGG analysis showed that pigs raised in semi free-grazing farm can activate the pathway of inosine monophosphate (IMP) biosynthesis, glycolysis/gluconeogenesis and alanine, aspartate and glutamate metabolism. Conclusions: Free range feeding improves meat quality by changing the fibre type, myofiber density and metabolic pathways related to flavour amino acids, IMP or glycolysis/gluconeogenesis in muscle. However, prolonged feeding cycle increases fat deposition and associated microbial communities. Keywords: Rearing pattern, Myofiber type, Fat deposition, Gut micobiota

Anti-obesity effects of Grifola frondosa through the modulation of lipid metabolism via ceramide in mice fed a high-fat diet

2021 ◽  
Author(s):  
Xue Jiang ◽  
Jie Hao ◽  
Zijian Liu ◽  
Xueting Ma ◽  
Yuxin Feng ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Lipid Metabolism ◽  
High Fat Diet ◽  
Fat Deposition ◽  
Grifola Frondosa ◽  
High Fat ◽  
Metabolic Complications ◽  
Basidiomycete Fungus

Obesity is characterized by massive fat deposition and is related to a series of metabolic complications, such as insulin resistance (IR) and steatohepatitis. Grifola frondosa (GF) is a basidiomycete fungus...

scholarly journals Key Genes Regulating Skeletal Muscle Development and Growth in Farm Animals

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 835
Author(s):  
Mohammadreza Mohammadabadi ◽  
Farhad Bordbar ◽  
Just Jensen ◽  
Min Du ◽  
Wei Guo
Keyword(s):  
Skeletal Muscle ◽  
Candidate Genes ◽  
Meat Quality ◽  
Muscle Development ◽  
Muscle Growth ◽  
Farm Animals ◽  
Meat Production ◽  
Skeletal Muscle Development ◽  
Extrinsic Factors ◽  
Myogenic Regulatory Factor

Farm-animal species play crucial roles in satisfying demands for meat on a global scale, and they are genetically being developed to enhance the efficiency of meat production. In particular, one of the important breeders’ aims is to increase skeletal muscle growth in farm animals. The enhancement of muscle development and growth is crucial to meet consumers’ demands regarding meat quality. Fetal skeletal muscle development involves myogenesis (with myoblast proliferation, differentiation, and fusion), fibrogenesis, and adipogenesis. Typically, myogenesis is regulated by a convoluted network of intrinsic and extrinsic factors monitored by myogenic regulatory factor genes in two or three phases, as well as genes that code for kinases. Marker-assisted selection relies on candidate genes related positively or negatively to muscle development and can be a strong supplement to classical selection strategies in farm animals. This comprehensive review covers important (candidate) genes that regulate muscle development and growth in farm animals (cattle, sheep, chicken, and pig). The identification of these genes is an important step toward the goal of increasing meat yields and improves meat quality.

scholarly journals High Fat Activates O-GlcNAcylation and Affects AMPK/ACC Pathway to Regulate Lipid Metabolism

Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1740
Author(s):  
Yuning Pang ◽  
Xiang Xu ◽  
Xiaojun Xiang ◽  
Yongnan Li ◽  
Zengqi Zhao ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
High Fat Diet ◽  
Lipid Synthesis ◽  
Fat Deposition ◽  
Liver Fat ◽  
Lipid Homeostasis ◽  
High Fat ◽  
Dual Inhibitor ◽  
Regulate Lipid Metabolism

A high-fat diet often leads to excessive fat deposition and adversely affects the organism. However, the mechanism of liver fat deposition induced by high fat is still unclear. Therefore, this study aimed at acetyl-CoA carboxylase (ACC) to explore the mechanism of excessive liver deposition induced by high fat. In the present study, the ORF of ACC1 and ACC2 were cloned and characterized. Meanwhile, the mRNA and protein of ACC1 and ACC2 were increased in liver fed with a high-fat diet (HFD) or in hepatocytes incubated with oleic acid (OA). The phosphorylation of ACC was also decreased in hepatocytes incubated with OA. Moreover, AICAR dramatically improved the phosphorylation of ACC, and OA significantly inhibited the phosphorylation of the AMPK/ACC pathway. Further experiments showed that OA increased global O-GlcNAcylation and agonist of O-GlcNAcylation significantly inhibited the phosphorylation of AMPK and ACC. Importantly, the disorder of lipid metabolism caused by HFD or OA could be rescued by treating CP-640186, the dual inhibitor of ACC1 and ACC2. These observations suggested that high fat may activate O-GlcNAcylation and affect the AMPK/ACC pathway to regulate lipid synthesis, and also emphasized the importance of the role of ACC in lipid homeostasis.

Does Hepatic Hepcidin Play an Important Role in Exercise-Associated Anemia in Rats?

2011 ◽  
Vol 21 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Yu-Qian Liu ◽  
Yan-Zhong Chang ◽  
Bin Zhao ◽  
Hai-Tao Wang ◽  
Xiang-Lin Duan
Keyword(s):  
Regulatory Mechanism ◽  
Iron Absorption ◽  
White Blood Cells ◽  
Strenuous Exercise ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Transcriptional Level ◽  
Divalent Metal Transporter 1 ◽  
Divalent Metal Transporter ◽  
Sports Anemia

Some athletes are diagnosed as suffering from sports anemia because of iron deficiency, but the regulatory mechanism remains poorly understood. It is reported that hepcidin may provide a way to illuminate the regulatory mechanism of exercise-associated anemia. Here the authors investigate the hepcidin-involved iron absorption in exercise-associated anemia. Twelve male Wistar rats (300 ± 10 g) were randomly divided into 2 groups, 6 in a control group (CG) and 6 in an exercise group (EG, 5 wk treadmill exercise of different intensities with progressive loading). Serum samples were analyzed for circulating levels of IL-6 by means of enzyme-linked immunosorbent assay (ELISA). The expression of hepatic hepcidin mRNA was examined by real-time polymerase chain reaction analysis. The protein levels of divalent metal transporter 1 (DMT1), ferroportin1 (FPN1), and heme-carrier protein 1 (HCP1) of duodenum epithelium were examined by Western blot. The results showed that the amount of iron and ferritin in serum were lower in EG than in CG (p < .05). The levels of IL-6 and white blood cells were greater in EG than in CG (p < .01). The expression of DMT1, HCP1, and FPN1 was significantly lower in EG than in CG (p < .01). The mRNA expressions of hepatic hepcidin and hemojuvelin in skeletal muscle were remarkably higher in EG than in CG. The data indicated that inflammation was induced by strenuous exercise, and as a result, the transcriptional level of the hepatic hepcidin gene was increased, which further inhibited the expression of iron-absorption proteins and led to exercise-associated anemia.

Gestational and lactational feeding strategies for gilts: Growth, carcass characteristics and meat quality of the progeny

2003 ◽  
Vol 83 (2) ◽  
pp. 205-211 ◽  
Author(s):  
A. Fortin ◽  
E. J. Clowes ◽  
A. L. Schaefer
Keyword(s):  
Meat Quality ◽  
National Research Council ◽  
Carcass Characteristics ◽  
Residual Effect ◽  
Feeding Strategies ◽  
Pork Quality ◽  
National Academy Of Sciences ◽  
Weaning Weight ◽  
Body Protein ◽  
Academy Of Sciences

This study was conducted to determine whether feeding gilts (1) at or above their National Academy of Sciences-National Research Council (NAS-NRC 1998) requirements during gestation, and (2) to lose a moderate (~10%) or large (~17%) amount of maternal protein during lactation had a residual effect on their progeny’s growth, carcass characteristics and pork quality at market weight. From each litter, the heaviest and lightest barrows and gilts were selected. The progeny of gilts fed above their requirements during gestation, and those that lost the least body protein during lactation were heavier at weaning; +0.3 kg (P < 0.05) and +0.5 kg (P = 0.01), respectively. However, these liveweight differences, which were associated with the gestation and lactation effects, were no longer evident (P > 0.05) at day 35 or 85 post-weaning. But at slaughter, these animals had thinner (P < 0.01) fat thickness and higher (P < 0.05) predicted salable meat yield. Independently of the gestation and lactation treatments, and compared to the low-weaning-weight pigs, the high- weaning-weight pigs maintained their weight advantage (P < 0.01 at day 35 (+ 2.8 kg) and day 85 (+ 5.4 kg) post-weaning), took 4.5 fewer days (P < 0.01) to reach market weight, but had similar (P > 0.05) carcass characteristics and pork quality. Key words: Gilts, gestational and lactational protein, litter, growth, carcass characteristics and meat quality

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