MiR-143 Targeting CUX1 to Regulate Proliferation of Dermal Papilla Cells in Hu Sheep

Wool curvature is the determining factor for lambskin quality of Hu lambs. However, the molecular mechanism of wool curvature formation is not yet known. MiRNA has been proved to play an important role in hair follicle development, and we have discovered a differentially expressed miRNA, miR-143, in hair follicles of different curl levels. In this study, we first examined the effects of miR-143 on the proliferation and cell cycle of dermal papilla cells using CCK8, EdU and flow cytometry and showed that miR-143 inhibited the proliferation of dermal papilla cells and slowed down the cell cycle. Bioinformatics analysis was performed to predict the target genes KRT71 and CUX1 of miR-143, and both two genes were expressed at significantly higher levels in small waves than in straight lambskin wool (p < 0.05) as detected by qPCR and Western blot (WB). Then, the target relationships between miR-143 and KRT71 and CUX1 were verified through the dual-luciferase assay in 293T cells. Finally, after overexpression and suppression of miR-143 in dermal papilla cells, the expression trend of CUX1 was contrary to that of miR-143. Meanwhile, KRT71 was not detected because KRT71 was not expressed in dermal papilla cells. Therefore, we speculated that miR-143 can target CUX1 to inhibit the proliferation of dermal papilla cells, while miR-143 can target KRT71 to regulate the growth and development of hair follicles, so as to affect the development of hair follicles and ultimately affect the formation of wool curvature.

Download Full-text

Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits

Bioscience Reports ◽

10.1042/bsr20191248 ◽

2020 ◽

Vol 40 (2) ◽

Cited By ~ 4

Author(s):

Zhenyu Wu ◽

Yanli Zhu ◽

Hongli Liu ◽

Gongyan Liu ◽

Fuchang Li

Keyword(s):

Cell Cycle ◽

Signaling Pathway ◽

Cell Cycle Progression ◽

Dermal Papilla ◽

Hair Shaft ◽

Hair Follicles ◽

Dermal Papilla Cells ◽

Root Sheath ◽

Inner Root Sheath

Abstract Wnt signaling plays an important role in the growth and development of hair follicles (HFs). Among the signaling molecules, Wnt10b was shown to promote the differentiation of primary skin epithelial cells toward the hair shaft and inner root sheath of the HF cells in mice in vitro. Whisker HFs were isolated from Rex rabbits and cultured in vitro to measure hair shaft growth. Meanwhile, dermal papilla cells (DPCs) were isolated and cultured in vitro. Treatment with AdWnt10b or the Wnt/β-Catenin Pathway inhibitor, XAV939, assessed the DPCs proliferation by CCK-8 assay. And the cell cycle was also analyzed by flow cytometry. We found that Wnt10b could promote elongation of the hair shaft, whereas XAV-939 treatment could eliminated this phenomenon. AdWnt10b treatment promoted the proliferation and induced G1/S transition of DPCs. AdWnt10b stimulation up-regulated β-Catenin protein in DPCs. Inhibition of Wnt/β-Catenin signaling by XAV-939 could decreased the basal and Wnt10b-enhanced proliferation of DPCs. And could also suppress the cell cycle progression in DPCs. In summary, our study demonstrates that Wnt10b could promote HFs growth and proliferation of DPCs via the Wnt/β-Catenin signaling pathway in Rex rabbits.

Download Full-text

Expression and distribution of EPHA4 and Ephrin A3 in Aohan fine-wool sheep skin

Archives Animal Breeding ◽

10.5194/aab-65-11-2022 ◽

2022 ◽

Vol 65 (1) ◽

pp. 11-19

Author(s):

Yu Cui ◽

Chunliang Wang ◽

Lirong Liu ◽

Nan Liu ◽

Jianning He

Keyword(s):

Hair Follicle ◽

Developmental Stages ◽

Quantitative Polymerase Chain Reaction ◽

Dermal Papilla ◽

Hair Follicles ◽

Fine Hair ◽

Dermal Papilla Cells ◽

Temporal Specificity ◽

Polymerase Chain ◽

Hair Follicle Development

Abstract. The objective of this study was to identify the expression and distribution of EPHA4 and Ephrin A3 genes in the development and morphogenesis of hair follicles in fine-wool sheep. The results could lay a theoretical basis for understanding the molecular mechanism that regulates hair follicle development. The skin of Aohan fine-wool sheep at different developmental stages (embryonic day 90, E90d, and 120, E120d, and postnatal day 1, B1d, and 30, B30d) were selected. Real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry were used to study the levels of mRNA and proteins, respectively. The RT-qPCR results showed that the mRNA expression level of EPHA4 at B1d was significantly lower than at E120d (p<0.01). The expression of Ephrin A3 at E120d was significantly higher than that at E90d and B1d (p<0.01). Immunohistochemical detection results showed that the level and localisation of EPHA4 and Ephrin A3 proteins had spatial and temporal specificity. EPHA4 expression in dermal papilla cells might be important for inducing Aohan fine-hair follicle regeneration and for controlling the properties of the hair. Ephrin A3 might play an important role in the redifferentiation of secondary hair follicles and might also be involved in the inhibition of apoptosis-related gene expression in hair follicles. The Ephrin A3 signalling pathway might accelerate the growth of fine-hair follicles and increase the density of hair follicles.

Download Full-text

Combinatorial expression of cell cycle regulators is more suitable for immortalization than oncogenic methods in dermal papilla cells

iScience ◽

10.1016/j.isci.2020.101929 ◽

2021 ◽

Vol 24 (1) ◽

pp. 101929

Author(s):

Tomokazu Fukuda ◽

Kai Furuya ◽

Kouhei Takahashi ◽

Ai Orimoto ◽

Eriko Sugano ◽

...

Keyword(s):

Cell Cycle ◽

Dermal Papilla ◽

Cell Cycle Regulators ◽

Dermal Papilla Cells ◽

Combinatorial Expression

Download Full-text

Hormones and Hair Growth: Variations in Androgen Receptor Content of Dermal papilla Cells Cultured from Human and Red Deer (Cervus Elaphus) Hair Follicles.

Journal of Investigative Dermatology ◽

10.1111/1523-1747.ep12363039 ◽

1993 ◽

Vol 101 (s1) ◽

pp. 114S-120S ◽

Cited By ~ 24

Author(s):

Valerie Anne Randall ◽

Margaret Julie Thornton ◽

Andrew Guy Messenger ◽

Nigel Andrew Hibberts ◽

Andrew Stewart Irving Loudon ◽

...

Keyword(s):

Androgen Receptor ◽

Cervus Elaphus ◽

Hair Growth ◽

Red Deer ◽

Dermal Papilla ◽

Hair Follicles ◽

Dermal Papilla Cells ◽

Cells Cultured

Download Full-text

Smooth muscle alpha-actin is a marker for hair follicle dermis in vivo and in vitro

Journal of Cell Science ◽

10.1242/jcs.99.3.627 ◽

1991 ◽

Vol 99 (3) ◽

pp. 627-636 ◽

Cited By ~ 2

Author(s):

C.A. Jahoda ◽

A.J. Reynolds ◽

C. Chaponnier ◽

J.C. Forester ◽

G. Gabbiani

Keyword(s):

Smooth Muscle ◽

Hair Follicle ◽

Dermal Papilla ◽

Hair Follicles ◽

Dermal Papilla Cells ◽

Smooth Muscle Alpha Actin ◽

Actin Expression ◽

Alpha Actin ◽

Sheath Cells

We have examined the expression of smooth muscle alpha-actin in hair follicles in situ, and in hair follicle dermal cells in culture by means of immunohistochemistry. Smooth muscle alpha-actin was present in the dermal sheath component of rat vibrissa, rat pelage and human follicles. Dermal papilla cells within all types of follicles did not express the antigen. However, in culture a large percentage of both hair dermal papilla and dermal sheath cells were stained by this antibody. The same cells were negative when tested with an antibody to desmin. Overall, explant-derived skin fibroblasts had relatively low numbers of positively marked cells, but those from skin regions of high hair-follicle density displayed more smooth muscle alpha-actin expression than fibroblasts from areas with fewer follicles. 2-D SDS-PAGE confirmed that, unlike fibroblasts, cultured papilla cells contained significant quantities of the alpha-actin isoform. The rapid switching on of smooth muscle alpha-actin expression by dermal papilla cells in early culture, contrasts with the behaviour of smooth muscle cells in vitro, and has implications for control of expression of the antigen in normal adult systems. The very high percentage of positively marked cultured papilla and sheath cells also provides a novel marker of cells from follicle dermis, and reinforces the idea that they represent a specialized cell population, contributing to the heterogeneity of fibroblast cell types in the skin dermis, and possibly acting as a source of myofibroblasts during wound healing.

Download Full-text

Inhibition of Rab27a and Rab27b Has Opposite Effects on the Regulation of Hair Cycle and Hair Growth

International Journal of Molecular Sciences ◽

10.3390/ijms21165672 ◽

2020 ◽

Vol 21 (16) ◽

pp. 5672

Author(s):

Kyung-Eun Ku ◽

Nahyun Choi ◽

Jong-Hyuk Sung

Keyword(s):

Hair Growth ◽

Expression Patterns ◽

Dermal Papilla ◽

Hair Follicles ◽

Hair Cycle ◽

Outer Root Sheath ◽

Dermal Papilla Cells ◽

Root Sheath ◽

Culture Models

Rab27a/b are known to play an important role in the transport of melanosomes, with their knockout causing silvery gray hair. However, the relationship between Rab27a/b and hair growth is not well known. To evaluate the role of Rab27a/b in hair cycle, we investigated the expression of Rab27a/b during hair cycling and human outer root sheath (hORS) cells. The expression of Rab27a in ORS cells was mainly detected at the anagen, whereas expression of Rab27b in ORS, and epidermal cells was strongly expressed at the telogen. Additionally, Rab27a/b were expressed in the Golgi of hORS cells. To evaluate the role of Rab27a/b in hair growth, telogen-to-anagen transition animal and vibrissae hair follicles (HFs) organ culture models were assayed using Rab27a/b siRNAs. The knockdown of Rab27a or Rab27b suppressed or promoted hair growth, respectively. These results were also confirmed in human dermal papilla cells (hDPCs) and hORS cells, showing the opposite mitogenic effects. Moreover, Rab27b knockdown increased the expression levels of various growth factors in the hDPCs and hORS cells. Overall, the opposite temporal expression patterns during hair cycling and roles for hair growth of Rab27a/b suggested that Rab27a/b might regulate the hair cycle. Therefore, our study may provide a novel solution for the development of hair loss treatment by regulating Rab27a/b levels.

Download Full-text

The local hypothalamic–pituitary–adrenal axis in cultured human dermal papilla cells

BMC Molecular and Cell Biology ◽

10.1186/s12860-020-00287-w ◽

2020 ◽

Vol 21 (1) ◽

Author(s):

Eun Young Lee ◽

You Jin Nam ◽

Sangjin Kang ◽

Eun Ju Choi ◽

Inbo Han ◽

...

Keyword(s):

Hpa Axis ◽

Hair Loss ◽

Human Hair ◽

Stress Hormones ◽

Dermal Papilla ◽

Hair Shaft ◽

Hair Follicles ◽

Follicle Cells ◽

Dermal Papilla Cells ◽

Underlying Mechanisms

Abstract Background Stress is an important cause of skin disease, including hair loss. The hormonal response to stress is due to the HPA axis, which comprises hormones such as corticotropin releasing factor (CRF), adrenocorticotropic hormone (ACTH), and cortisol. Many reports have shown that CRF, a crucial stress hormone, inhibits hair growth and induces hair loss. However, the underlying mechanisms are still unclear. The aim of this study was to examine the effect of CRF on human dermal papilla cells (DPCs) as well as hair follicles and to investigate whether the HPA axis was established in cultured human DPCs. Results CRF inhibited hair shaft elongation and induced early catagen transition in human hair follicles. Hair follicle cells, both human DPCs and human ORSCs, expressed CRF and its receptors and responded to CRF. CRF inhibited the proliferation of human DPCs through cell cycle arrest at G2/M phase and induced the accumulation of reactive oxygen species (ROS). Anagen-related cytokine levels were downregulated in CRF-treated human DPCs. Interestingly, increases in proopiomelanocortin (POMC), ACTH, and cortisol were induced by CRF in human DPCs, and antagonists for the CRF receptor blocked the effects of this hormone. Conclusion The results of this study showed that stress can cause hair loss by acting through stress hormones. Additionally, these results suggested that a fully functional HPA axis exists in human DPCs and that CRF directly affects human DPCs as well as human hair follicles under stress conditions.

Download Full-text