scholarly journals Isolation and Characterization of Crude Oil Degrading Bacteria in Association with Microalgae in Saver Pit from Egbaoma Flow Station, Niger Delta, Nigeria

2020 ◽  
Vol 2 (2) ◽  
pp. 12-16
Author(s):  
Obhioze Augustine Akpoka
Keyword(s):  
Crude Oil ◽  
Heterotrophic Bacteria ◽  
Oil Pollution ◽  
Heterotrophic Bacterium ◽  
Bacterial Species ◽  
Carbon Sources ◽  
Sole Source ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Hydrocarbon Utilizing Bacteria

The capability of indigenous bacteria and microalgae in crude oil effluents to grow in and utilize crude oil as their sole source of carbon and energy provides an environmentally friendly and economical process for dealing with crude oil pollution and its inherent hazards. In view of the toxicity of crude oil spillages to indwellers of the affected ecosystems and the entire affected environment, the isolation of pure bacterial and microalgae cultures from crude effluents is a step in the right direction, particularly for bio-augmentation or bioremediation purposes. The total heterotrophic bacteria count and hydrocarbon utilizing bacteria count, as well as the microalgae count, were determined with the pour plate technique. The physicochemical properties of the effluent samples were also analyzed. Identification of the hydrocarbon utilizing bacteria was performed with phenotypic techniques. The result shows a mean total heterotrophic bacterium count of 5.91 log CFU/ml and a mean microalga count of 4.77 log cells/ml. When crude oil and polycyclic aromatic hydrocarbon (PAH) were used as sole carbon sources, total hydrocarbon utilizing bacteria counts were respectively estimated at 3.89 and 2.89 log CFU/ml. Phenotypic identification of hydrocarbon utilizing bacteria in the crude oil effluents revealed the presence of two main bacterial genera: Streptococcus and Pseudomonas. Data obtained from this study confirmed the biodegradative abilities of indigenous bacterial species, thus, ultimately resulting in the amelioration of the toxicity associated with the crude oil effluents.

Isolation and Characterization of Hydrocarbon-degrading Bacteria in Soils of Mechanical Workshops in Maiduguri, Borno State

2021 ◽  
Vol 4 (2) ◽  
pp. 35-38
Author(s):  
Hussaini Shettima ◽  
Ibrahim Alkali Allamin ◽  
Nasir Halima ◽  
Haruna Yahya Ismail ◽  
Yusuf Musa
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Heterotrophic Bacteria ◽  
Bacterial Species ◽  
Bacillus Coagulans ◽  
Engine Oil ◽  
Klebsiella Pneumonia ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Hydrocarbon Utilizing Bacteria

The study was conducted to determine the distribution of hydrocarbon utilizing bacteria in spent engine oil (SEO) contaminated soil. Five mechanical workshops within Maiduguri Metropolis. Five bulk soil sample comprising of one each of the five sites; the sites are Leventis Area on Kashim Ibrahim Way, Damboa Raod, Boiler (commonly called Bola) near Maiduguri Monday Market, Ngomari, on Kano Road, and 1000 Housing Estate, also on Kano Road. Nutrient agar was used, or isolation and enumeration total heterotrophic bacteria and Mineral salt agar was used for the isolation and enumeration of hydrocarbon utilizing bacteria. The result shows THB (92.0x104) at Damboa road (DR) to as higher and (78.8x104) at Bola area (BL) while HUB (2.0x104) at Damboa road (DR) and as high as (9.3x104) at Bola area (BL). The bacterial species isolated were species of Bacillus licheniformis, Bacillus subtilis, Bacillus coagulans, Bacillus alvei, Bacillus cereus, Bacillus lentus other are Pseudomonas aeruginosa, Klebsiella pneumonia. Bacillus licheniformis, and Bacillus subtilis are the most occurring bacterial isolates identified. The ability of those bacterial isolate to degrade hydrocarbon buoyantly will help in remediation of oil polluted environments.

scholarly journals Occurrence of hydrocarbon degrading bacteria in soil in Kukawa, Borno State

2014 ◽  
Vol 3 (2) ◽  
pp. 36-47 ◽  
Author(s):  
IA Allamin ◽  
UJJ Ijah ◽  
HY Ismail ◽  
ML Riskuwa
Keyword(s):  
Crude Oil ◽  
Heterotrophic Bacteria ◽  
Bacterial Species ◽  
Petroleum Exploration ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
As Species ◽  
Degrading Bacteria ◽  
Different Depths ◽  
Hydrocarbon Utilizing Bacteria

Soil samples were collected from five sites covering petroleum exploration station in Kukawa, Kukawa Local Government Area of Borno State, Nigeria between October, 2012 and February, 2013 at two different depths (0-10cm and 10-20cm) to enumerate and identify hydrocarbon degrading bacteria in the soil. Total aerobic heterotrophic bacteria (TAHB) were enumerated on Nutrient agar (NA), and Hydrocarbon utilizing bacteria (HUB) enumerated on Oil agar (OA). The bacterial isolates were identified using morphological and biochemical tests. It was observed that the microorganisms (TAHB, and HUB) were more densely populated at 10cm depth. (TAHB: 5.3×108 - 11.4×108cfu/g, and HUB: 2.4×105 - 5.3×105 cfu/g, than at 20 cm depth (TAHB: 3.0×108 - 5.7×108 cfu/g, and HUB: 2.1×105 - 4.8×105 cfu/g). The HUB was identified as species of Bacillus, Pseudomonas, Klebsiella, Lactobacillus, Micrococcus, Corynebacterium, and Actinomyces. Bacillus, and Pseudomonas species were more constantly isolated than other isolates and they constitute 100% of total bacterial isolates. The potential of hydrocarbon utilizing bacteria isolated to degrade hydrocarbon was studied. Nineteen (19) bacterial species was screened, Bacillus subtilis, Pseudomonas aeruginosa, Bacillus cereus, Klebsiella pneumoniae, Micrococcus leteus,and Lactobacillus casei, utilized and degrade crude oil at considerably high rates after 21 days of incubation. The degradation efficiency was confirmed by GC-MS analysis, which indicated that the bacterial isolates utilized most of the crude oil components particularly straight chain alkanes and cycloalkanes DOI: http://dx.doi.org/10.3126/ije.v3i2.10503 International Journal of the Environment Vol.3(2) 2014: 36-47

scholarly journals Characterization of Indigenous Bacterial Communities in Crude-Oil Impacted Sites at Obagi Town, Onelga, Rivers State, Nigeria

Fine Focus ◽  
2016 ◽  
Vol 2 (1) ◽  
pp. 7-14
Author(s):  
Chioma Blaise Chikere ◽  
Chinyere Augusta Ajuzieogu ◽  
Michael Chukwugoziem Miller
Keyword(s):  
Crude Oil ◽  
Heterotrophic Bacteria ◽  
Bacterial Species ◽  
Polluted Soil ◽  
Soil Samples ◽  
Isolation And Characterization ◽  
Significant Difference ◽  
Rivers State ◽  
Hydrocarbon Utilizing Bacteria

Hydrocarbon utilizers are expected to be indigenous in crude-oil polluted environments. The isolation and characterization of hydrocarbon utilizers is often a key strategy in bioremediation of hydrocarbon-polluted environments. In this study, crude-oil polluted soil samples from Obagi town, Onelga, Rivers state were enumerated and characterized for putative hydrocarbon utilizing bacterial populations. Biochemical characterization identified five bacterial species representative of five genera: Bacillus, Pseudomonas, Acinetobacter, Micrococcus and Staphylococcus. Amongst the genera of bacteria isolated, Bacillus had the highest frequency of occurrence (40%). The mean count of total heterotrophic bacteria was 1.7 X 107 cfu/g, while hydrocarbon utilizing bacteria (HUB) count mean density was 1.0 X 107 cfu/g for the three soil samples. Statistical analyses revealed no significant difference at p>0.05 between Total Heterotrophic Bacterial (THB) and Hydrocarbon Utilizing Bacterial (HUB) counts, suggesting that most of the bacteria present in the sampled sites were hydrocarbon utilizers. Findings from this study suggest the presence of indigenous putative hydrocarbon utilizing bacteria in the crude-oil polluted soil of Obagi town. Hence, a promising potential exists for future bioremediation studies on the site.

scholarly journals Characterization of Hydrocarbon Utilizing Bacteria and Fungi Associated with Crude Oil Contaminated Soil

2020 ◽  
pp. 54-69
Author(s):  
D. N. Ogbonna ◽  
S. I. Douglas ◽  
V. G. Awari
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Heterotrophic Bacteria ◽  
Anthropogenic Activities ◽  
Bacterial Species ◽  
Soil Conditions ◽  
Penicillium Species ◽  
Hydrocarbon Utilizing Bacteria

Many substances known to have toxic properties are regularly introduced into the environment through human activity. These substances which include hydrocarbons range in degree of toxicity and danger to human health. Frequent oil spills incidents have become a problem to ecological protection efforts. Conventional methods to remove, reduce or mitigate toxic substances introduced into soil via anthropogenic activities suffer setbacks due to the level of risk involved but bioremediation offers an alternative method to detoxify contaminants especially if the soil conditions are amended with organic nutrients or growth enhancing co-substrates. This study was therefore aimed characterizing hydrocarbon utilizing microorganisms associated with crude oil contaminated soils. Soils were obtained from the Rivers State University Agricultural farm contaminated deliberately with crude oil and allowed for 21 days to mimic the natural polluted soil. Sample collection and analyses were carried out according to standard microbiological procedures while characterization of the isolates was done using genomic studies. The results of microbial counts obtained from the soil samples for total heterotrophic bacteria ranged from 2.10 x108 to 2.58 x108 cfu/g, Total heterotrophic fungi had 1.6 x105 to 2.0 x105 cfu/g while the hydrocarbon utilizing bacteria ranged from 8.0 x103 to 5.0 x104 cfu/g and total hydrocarbon utilizing fungi ranged from 9.0 x103 to 7.0 x104 cfu/g in the contaminated soil. Five hydrocarbon utilizing bacterial species were identified as Staphylococcus saprophyticus, Bacillus amyloliquefaciens, Pseudomonas aeruginosa, Comamonas testosteroni and Chryseobacterium cucumeris while five hydrocarbon utilizing fungal species were identified as Penicillium citrinum, Penicillium brocae, Fusarium solani, Kodamaea ohmeri and Lentinus squarrosulus. Bacillus and Penicillium species were predominantly isolated from the soil. This may be due to the ability of the organisms to produce spores, which may shield them from the toxic effects of the hydrocarbons. Since these organisms are able to utilize crude oil as their sole carbon source. Hence, can be used for bioremediation of crude oil polluted environment.

scholarly journals ISOLATION AND CHARACTERIZATION OF LIPOLYTIC BACTERIA FROM OIL CONTAMINATED SOIL FROM PETROL BUNK AT SOUTHEAST BANGALORE.

2019 ◽  
Vol 7 (2) ◽  
pp. 30-34
Author(s):  
Ajay Kumar Sahu ◽  
Rahul Nemani ◽  
Prangya Prangya Acharya ◽  
Rupali Sinha ◽  
Subhranil Sengupta ◽  
...  
Keyword(s):  
Crude Oil ◽  
Soil Sample ◽  
Contaminated Soil ◽  
Bacterial Species ◽  
Carbon Sources ◽  
Lubricating Oil ◽  
Contaminated Sites ◽  
Gravimetric Analysis ◽  
Morphological Studies ◽  
Isolation And Characterization

The isolation of oil contaminated sites and gravimetric analysis of degradation in which, two bacterial formed maximum clearing zones on minerals salt medium. An increase in cell number indicating that the bacterial isolation was responsible for the oil degradation .the collected oil contaminated sites at kormongala, Indra nagar, MTTC culture , micrococcus spp., Bacillus spp., pseudomonas spp., which are able to utilize the oil in soil as carbon sources, were added to oil contaminated soil sample , the growth profiles were determined by monitoring the optical density, dry weight and Ph of the culture utilizing lubricating oil as sole sources of carbon, Bushnell  Haas media supplemented with petrol, kerosene and diesel as sole carbon sources was used for isolation of bacteria capable of degrading these petroleum fractionates. From three soil sample and two water sample, a total of nine bacterial strains were isolated capable of degrading petrol, kerosene and diesel with varying tolerance capacities, the isolates were identify by using standard biochemical test and morphological studies and it was determined that these strains belong to six bacterial genera .the present study suggest that the isolated bacterial species could be employed for bioremediation in environment polluted with petroleum and its products, indigenously from the soil and water contaminated with crude oil in the vicinity of oil drilling well were found to be most efficient crude oil utilize as turbidity observed by spectrophotometrically. In the various study of lipolytic bacteria concluded that the taken of oil contaminated soil from from petroleum bunk and to identify their biochemical characterization by using various sources. How it’s helpful for characterize by using of lipolytic bacteria

scholarly journals Isolation and Characterization of a Biosurfactant Producing Strain Planococcus sp. XW-1 from the Cold Marine Environment

2022 ◽  
Vol 19 (2) ◽  
pp. 782
Author(s):  
Ping Guo ◽  
Weiwei Xu ◽  
Shi Tang ◽  
Binxia Cao ◽  
Danna Wei ◽  
...  
Keyword(s):  
Low Temperature ◽  
Carbon Source ◽  
Crude Oil ◽  
Surface Activity ◽  
Yellow Sea ◽  
High Surface ◽  
Sole Source ◽  
The Yellow Sea ◽  
Isolation And Characterization ◽  
First Time

One cold-adapted strain, named Planococcus sp. XW-1, was isolated from the Yellow Sea. The strain can produce biosurfactant with petroleum as sole source of carbon at low temperature (4 °C). The biosurfactant was identified as glycolipid-type biosurfactant species by thin-layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR). It reduced the surface tension of water to 26.8 mN/m with a critical micelle concentration measurement of 60 mg/L. The produced biosurfactant possesses high surface activity at wide ranges of temperature (−18–105 °C), pH values (2–12), and salt concentrations (1–18%). The biosurfactant exhibited higher surface activity and higher growth rate of cells with hexadecane and diesel as carbon source. The strain Planococcus sp. XW-1 was also effective in degrading crude oil, after 21 days of growth at 4 °C in medium with 1% crude oil and 1% (v/v) bacteria broth, 54% of crude oil was degraded. The results suggest that Planococcus sp. XW-1 is a promising candidate for use in the bioremediation of petroleum-contaminated seawater in the Yellow Sea during winter. This study reported for the first time that Planococcus isolated from the Yellow Sea can produce biosurfactant using petroleum as the sole carbon source at low temperature (4 °C), showing its ecological role in the remediation of marine petroleum pollution.

scholarly journals Biotreatability of Crude Oil Polluted Aquatic Environment Using Indigenous Hydrocarbon Utilizing Bacteria

2020 ◽  
pp. 52-62
Author(s):  
Tudararo-Aherobo Laurelta ◽  
Okotie Sylvester ◽  
Ataikiru Tega ◽  
Stephen Avwerosuoghene
Keyword(s):  
Crude Oil ◽  
Contaminated Soils ◽  
Aquatic Environment ◽  
Bacterial Counts ◽  
A Value ◽  
Degrading Bacteria ◽  
Petroleum Resources ◽  
Oil Concentration ◽  
American Society ◽  
Hydrocarbon Utilizing Bacteria

Aim: The research aims to assess the biodegradability of crude oil polluted aquatic environment using indigenous hydrocarbon degrading bacteria. Place and Duration of Study: The research was conducted in the Environmental Management and Toxicology Laboratory, Federal University of Petroleum Resources, Effurun, Delta State. Methodology: Hydrocarbon degrading bacteria species were isolated from hydrocarbon contaminated soils, screened and used for the degradation of crude oil. 5% and 10% crude oil were used to spike the test microcosm. Physicochemical parameters such as, pH, turbidity, total petroleum hydrocarbon (TPH) and bacterial counts of the bioremediated crude oil contaminated water were monitored on Day 0, 7 and 14. The biodegradation of the crude oil was done with the various bacteria isolates singly and as a consortium. Standard methods of American Public Health Association (APHA) and American Society for Testing and Materials (ASTM) were used for the analysis. Results: The isolates identified and used for the biodegradation process were, Azomonas sp., Enterococcus sp., Klebsiella sp. and Rhizobactersp. On day 14, in the microcosms with 5% crude oil contamination, Azomonas sp. recorded the highest turbidity reading of 328 ± 2.0 NTU, while Rhizobacter sp. recorded the least with 57.67 ± 0.58 NTU. The bacterial countswere between 7.68 ± 0.002 CFU/ml and 8.05 ± 0.10x 107 CFU/ml for Rhizobacter sp. and Azomonas sp. respectively.The crude oil was also degraded most in the microcosm treated with Azomonas sp. with a residual TPH concentration of 0.0013± 0.005 mg/l.For the 10% crude oil contaminated microcosms, TPH was also biodegraded most by Azomonas sp. with a value of 0.0026 ± 0.002mg/l. Turbidity readings were between 82 ± 1.0 NTU and 375.33 ± 0.57 NTU for Rhizobacter sp. and Azomonas sp. respectively. Bacterial counts were between (7.71± 0.012)x 107CFU/ml – (8.13± 0.001) x 107CFU/ml for Rhizobacter sp. and Azomonassp. respectively. Conclusion:There wasincreased microbial countsand decrease of residual crude oil concentration, indicating degradation of the crude oil by all the isolates.However, Azomonas sp. recorded the highest TPH degradation for both the 5% and 10% crude oil contaminated microcosms.Thus, findings from the research indicate that hydrocarbon degrading bacteria exist in our environment and can be used in the remediation of aquatic polluted environment.

Isolation and Characterization of Biosurfactant Producing and Crude Oil Degrading Bacteria from Oil Contaminated Soils

2017 ◽  
Vol 42 (3) ◽  
pp. 1149-1156 ◽  
Author(s):  
Ali Ebadi ◽  
Mohsen Olamaee ◽  
Nayer Azam Khoshkholgh Sima ◽  
Reza Ghorbani Nasrabadi ◽  
Maryam Hashemi
Keyword(s):  
Crude Oil ◽  
Contaminated Soils ◽  
Isolation And Characterization ◽  
Degrading Bacteria

scholarly journals Isolation and Characterization of a Genetically Tractable Photoautotrophic Fe(II)-Oxidizing Bacterium, Rhodopseudomonas palustris Strain TIE-1

2005 ◽  
Vol 71 (8) ◽  
pp. 4487-4496 ◽  
Author(s):  
Yongqin Jiao ◽  
Andreas Kappler ◽  
Laura R. Croal ◽  
Dianne K. Newman
Keyword(s):  
Bacterial Species ◽  
Rhodopseudomonas Palustris ◽  
Carbon Sources ◽  
Integral Membrane Protein ◽  
Transposition Frequency ◽  
Isolation And Characterization ◽  
Abc Transport ◽  
A Cell ◽  
Random Insertion

ABSTRACT We report the isolation and characterization of a phototrophic ferrous iron [Fe(II)]-oxidizing bacterium named TIE-1 that differs from other Fe(II)-oxidizing phototrophs in that it is genetically tractable. Under anaerobic conditions, TIE-1 grows photoautotrophically with Fe(II), H2, or thiosulfate as the electron donor and photoheterotrophically with a variety of organic carbon sources. TIE-1 also grows chemoheterotrophically in the dark. This isolate appears to be a new strain of the purple nonsulfur bacterial species Rhodopseudomonas palustris, based on physiological and phylogenetic analysis. Fe(II) oxidation is optimal at pH 6.5 to 6.9. The mineral products of Fe(II) oxidation are pH dependent: below pH 7.0 goethite (α-FeOOH) forms, and above pH 7.2 magnetite (Fe3O4) forms. TIE-1 forms colonies on agar plates and is sensitive to a variety of antibiotics. A hyperactive mariner transposon is capable of random insertion into the chromosome with a transposition frequency of ∼10−5. To identify components involved in phototrophic Fe(II) oxidation, mutants of TIE-1 were generated by transposon mutagenesis and screened for defects in Fe(II) oxidation in a cell suspension assay. Among approximately 12,000 mutants screened, 6 were identified that are specifically impaired in Fe(II) oxidation. Five of these mutants have independent disruptions in a gene that is predicted to encode an integral membrane protein that appears to be part of an ABC transport system; the sixth mutant has an insertion in a gene that is a homolog of CobS, an enzyme involved in cobalamin (vitamin B12) biosynthesis.

Microflore hétérotrophe impliquée dans le procédé simultané de biolixiviation des métaux et de digestion des boues d'épuration

2001 ◽  
Vol 28 (1) ◽  
pp. 158-174 ◽  
Author(s):  
M Gamache ◽  
J F Blais ◽  
R D Tyagi ◽  
N Meunier
Keyword(s):  
Sewage Sludge ◽  
Heterotrophic Bacteria ◽  
Bacterial Species ◽  
Microbial Populations ◽  
Primary Sludge ◽  
Sludge Digestion ◽  
Isolation And Characterization ◽  
Secondary Sludge ◽  
Blastoschizomyces Capitatus

Until now, some microbiological studies have made it possible to highlight the role and identification of certain chimiolithotroph microorganisms directly involved in the simultaneous sewage sludge digestion and metal leaching (SSDML process). Moreover, some other studies have established the performance of the SSDML process for the destruction of pathogens. However, until now no study has been carried out to define the equilibrium of the heterotrophic microbial populations during the sludge acidification occurring during the operation of the SSDML process. Hence, the SSDML process was studied in batch and continuous mode employing different types of sludges (Communauté Urbaine de Québec (CUQ)-East primary sludge, Beauceville secondary sludge, and mixture of primary and secondary Valcartier sludge). The equilibrium of heterotrophic microbial populations was investigated along with sludge acidification and solids degradation. The results obtained during the SSDML process on different sludges showed a decrease followed by the destruction of a majority of heterotrophic bacterial species found in fresh sludges. The isolation and characterization of different microbial species showed that only two types of microorganisms persisted: the yeast Blastoschizomyces capitatus and an unidentified fungus.Key words: sewage sludge, simultanous bioleaching and digestion, heterotrophic bacteria, Blastoschizomyces capitatus, fungi.

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