scholarly journals Structural Identification of Antibacterial Lipids from Amazonian Palm Tree Endophytes through the Molecular Network Approach

2019 ◽  
Vol 20 (8) ◽  
pp. 2006 ◽  
Author(s):  
Morgane Barthélemy ◽  
Nicolas Elie ◽  
Léonie Pellissier ◽  
Jean-Luc Wolfender ◽  
Didier Stien ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Structural Identification ◽  
Chemical Study ◽  
Palm Tree ◽  
Tandem Mass ◽  
Network Approach ◽  
Ion Clusters ◽  
Ethyl Acetate Extracts

A library of 197 endophytic fungi and bacteria isolated from the Amazonian palm tree Astrocaryum sciophilum was extracted and screened for antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). Four out of five antibacterial ethyl acetate extracts were also cytotoxic for the MRC-5 cells line. Liquid chromatography coupled to tandem mass spectrometry (UPHLC-HRMS/MS) analyses combined with molecular networking data processing were carried out to allow the identification of depsipeptides and cyclopeptides responsible for the cytotoxicity in the dataset. Specific ion clusters from the active Luteibacter sp. extract were also highlighted using an MRSA activity filter. A chemical study of Luteibacter sp. was conducted leading to the structural characterization of eight fatty acid exhibiting antimicrobial activity against MRSA in the tens of µg/mL range.

scholarly journals Identification and dereplication of endophytic Colletotrichum strains by MALDI TOF mass spectrometry and molecular networking

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Morgane Barthélemy ◽  
Vincent Guérineau ◽  
Grégory Genta-Jouve ◽  
Mélanie Roy ◽  
Jérôme Chave ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chemical Study ◽  
Chemical Diversity ◽  
Biologically Active ◽  
Ionization Time ◽  
Ion Clusters ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Palm Species ◽  
Ethyl Acetate Extracts

AbstractThe chemical diversity of biologically active fungal strains from 42 Colletotrichum, isolated from leaves of the tropical palm species Astrocaryum sciophilum collected in pristine forests of French Guiana, was investigated. The collection was first classified based on protein fingerprints acquired by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) correlated with cytotoxicity. Liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-HRMS/MS) data from ethyl acetate extracts were acquired and processed to generate a massive molecular network (MN) using the MetGem software. From five Colletotrichum strains producing cytotoxic specialized metabolites, we predicted the occurrence of peptide and cytochalasin analogues in four of them by MN, including a similar ion clusters in the MN algorithm provided by MetGem software. Chemoinformatics predictions were fully confirmed after isolation of three pentacyclopeptides (cyclo(Phe-Leu-Leu-Leu-Val), cyclo(Phe-Leu-Leu-Leu-Leu) and cyclo(Phe-Leu-Leu-Leu-Ile)) and two cytochalasins (cytochalasin C and cytochalasin D) exhibiting cytotoxicity at the micromolar concentration. Finally, the chemical study of the last active cytotoxic strain BSNB-0583 led to the isolation of four colletamides bearing an identical decadienamide chain.

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