Effect of S-Allyl –L-Cysteine on MCF-7 Cell Line 3-Mercaptopyruvate Sulfurtransferase/Sulfane Sulfur System, Viability and Apoptosis

The S-Allyl-L-cysteine (SAC) component of aged garlic extract (AGE) is proven to have anticancer, antihepatotoxic, neuroprotective and neurotrophic properties. γ-Cystathionase (CTH), cystathionine β-synthase (CBS) and 3-mercaptopyruvate sulfurtransferase (MPST) are involved in H2S/sulfane sulfur endogenous formation from L-cysteine. The aim of the study was to determine the effect of SAC on MCF-7 cells survival and apoptosis, which is a widely known approach to reduce the number of cancer cells. An additional goal of this paper was to investigate the effect of SAC on the activity and expression of enzymes involved in H2S production. The experiments were carried out in the human breast adenocarcinoma cell line MCF-7. Changes in the cell viability were determined by MTT assay. Cell survival was determined by flow cytometry (FC). Changes in enzymes expression were analyzed using Western blot. After 24 h and 48 h incubation with 2245 µM SAC, induction of late apoptosis was observed. A decrease in cell viability was observed with increasing SAC concentration and incubation time. SAC had no significant cytotoxic effect on the MCF-7 cells upon all analyzed concentrations. CTH, MPST and CBS expression were confirmed in non-treated MCF-7 cells. Significant decrease in MPST activity at 2245 µM SAC after 24 h and 48 h incubation vs. 1000 µM SAC was associated with decrease in sulfane sulfur levels. The presented results show promising SAC effects regarding the deterioration of the MCF-7 cells’ condition in reducing their viability through the downregulation of MPST expression and sulfate sulfur level reduction.

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Menadione effect on l-cysteine desulfuration in U373 cells.

Acta Biochimica Polonica ◽

10.18388/abp.2007_3263 ◽

2007 ◽

Vol 54 (2) ◽

pp. 407-411 ◽

Cited By ~ 8

Author(s):

Maria Wróbel ◽

Halina Jurkowska

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Control Culture ◽

Oxygen Species ◽

Active Centers ◽

Glutathione Synthesis ◽

Sulfane Sulfur ◽

Mercaptopyruvate Sulfurtransferase ◽

Sulfur Level ◽

U373 Cells

The non-cytotoxic concentration (20 microM) of menadione (2-methyl-1,4-naphthoquinone), after 1 h of incubation, leads to loss of the activity of rhodanese by 33%, 3-mercaptopyruvate sulfurtransferase by 20%, as well as the level of sulfane sulfur by about 23% and glutathione by 12%, in the culture of U373 cells, in comparison with the control culture. Reactive oxygen species generated by menadione oxidize sulfhydryl groups in active centers of the investigated enzymes, inhibiting them and saving cysteine for glutathione synthesis. A decreased sulfane sulfur level can be correlated with an oxidative stress.

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Effect of Hydroalcoholic Extract of Ephedramajor, Memordia charantia, and Resveratrol on Cytotoxicity and Caspase-3 Genes Expression Level in MCF-7 Breast Cancer Cell Line

Gene Cell and Tissue ◽

10.5812/gct.110658 ◽

2021 ◽

Vol In Press (In Press) ◽

Author(s):

Seyed Kazem Sabbagh ◽

Ehsan Ghodrati ◽

Alireza Hajibeiki ◽

Mahta Mazaheri ◽

Mohammad Reza Sarafraz Ardakani ◽

...

Keyword(s):

Gene Expression ◽

Medicinal Plants ◽

Cell Viability ◽

Cell Line ◽

Plant Extracts ◽

Caspase 3 ◽

Cell Toxicity ◽

Hydroalcoholic Extract ◽

Expression Levels ◽

Mcf 7

Background: To increase the therapeutic effect of drugs to combat diseases, combination therapy with current chemical drugs and new medicines derived from medicinal plants is necessary. Objectives: The present work aimed to investigate the effect of hydroalcoholic extract of two medicinal plants, Ephedra major and Momordi cacharantia (Carla), and resveratrol drug on cell viability and expression levels of caspase-3 gene in MCF-7 cell line. Methods: In this experimental study, the hydroalcoholic extraction of tested plants was done with a Soxhlet extractor. The MTT assay and real-time PCR were used to determine cell toxicity and caspase-3 gene expression levels, respectively. Results: The highest and lowest cytotoxic effects of plant extracts and resveratrol were observed at concentrations of 500 and 150 µg/mL, respectively. The highest level of the caspase-3 gene expression was observed after 72 h of incubation by different concentrations of plant extracts and resveratrol. Conclusions: It can be concluded that both plant extracts could influence cell viability in MCF-7 cells via the increase of cell toxicity and expression of caspase3 gene. Thus, these species could be used in the pharmaceutical industry.

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XIAP inhibitor and antiestrogen embelin abrogates metastasis and augments apoptosis in estrogen receptor positive human breast adenocarcinoma cell line MCF-7

Molecular Biology Reports ◽

10.1007/s11033-013-2938-z ◽

2014 ◽

Vol 41 (2) ◽

pp. 935-946 ◽

Cited By ~ 7

Author(s):

K. R. Sumalatha ◽

G. Abiramasundari ◽

G. K. Chetan ◽

T. Divya ◽

G. Sudhandiran ◽

...

Keyword(s):

Estrogen Receptor ◽

Cell Line ◽

Breast Adenocarcinoma ◽

Human Breast ◽

Adenocarcinoma Cell Line ◽

Adenocarcinoma Cell ◽

Human Breast Adenocarcinoma ◽

Estrogen Receptor Positive ◽

Mcf 7

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Genetic variability in a frozen batch of MCF-7 cells invisible in routine authentication affecting cell function

Scientific Reports ◽

10.1038/srep28994 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 42

Author(s):

Andre Kleensang ◽

Marguerite M. Vantangoli ◽

Shelly Odwin-DaCosta ◽

Melvin E. Andersen ◽

Kim Boekelheide ◽

...

Keyword(s):

Cell Line ◽

Genetic Heterogeneity ◽

Cell Function ◽

Breast Adenocarcinoma ◽

Comparative Genomic ◽

Cell Bank ◽

Cell Line Authentication ◽

Str Markers ◽

Genetic Drifts ◽

Mcf 7

Abstract Common recommendations for cell line authentication, annotation and quality control fall short addressing genetic heterogeneity. Within the Human Toxome Project, we demonstrate that there can be marked cellular and phenotypic heterogeneity in a single batch of the human breast adenocarcinoma cell line MCF-7 obtained directly from a cell bank that are invisible with the usual cell authentication by short tandem repeat (STR) markers. STR profiling just fulfills the purpose of authentication testing, which is to detect significant cross-contamination and cell line misidentification. Heterogeneity needs to be examined using additional methods. This heterogeneity can have serious consequences for reproducibility of experiments as shown by morphology, estrogenic growth dose-response, whole genome gene expression and untargeted mass-spectroscopy metabolomics for MCF-7 cells. Using Comparative Genomic Hybridization (CGH), differences were traced back to genetic heterogeneity already in the cells from the original frozen vials from the same ATCC lot, however, STR markers did not differ from ATCC reference for any sample. These findings underscore the need for additional quality assurance in Good Cell Culture Practice and cell characterization, especially using other methods such as CGH to reveal possible genomic heterogeneity and genetic drifts within cell lines.

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Assessment of synergistic effect of combining hyperthermia with irradiation and calcium carbonate nanoparticles on proliferation of human breast adenocarcinoma cell line (MCF-7 cells)

Artificial Cells Nanomedicine and Biotechnology ◽

10.1080/21691401.2018.1457537 ◽

2018 ◽

Vol 46 (sup2) ◽

pp. 364-372 ◽

Cited By ~ 7

Author(s):

Nasrollah Jabbari ◽

Leila Zarei ◽

Hadi Esmaeili Govarchin Galeh ◽

Bahman Mansori Motlagh

Keyword(s):

Calcium Carbonate ◽

Synergistic Effect ◽

Cell Line ◽

Breast Adenocarcinoma ◽

Human Breast ◽

Adenocarcinoma Cell Line ◽

Adenocarcinoma Cell ◽

Human Breast Adenocarcinoma ◽

Mcf 7

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Raman Spectroscopy: An Exploratory Study to Identify Post-Radiation Cell Survival

Applied Spectroscopy ◽

10.1177/0003702820908352 ◽

2020 ◽

Vol 74 (5) ◽

pp. 553-562

Author(s):

Kshama Pansare ◽

Saurav Raj Singh ◽

Venkatavaradhan Chakravarthy ◽

Neha Gupta ◽

Arti Hole ◽

...

Keyword(s):

Raman Spectroscopy ◽

Cell Viability ◽

Cell Survival ◽

Cell Line ◽

Cell Lines ◽

Clonogenic Assay ◽

Early Stage ◽

Raman Band ◽

Breast Adenocarcinoma ◽

Linear Discriminant

Resistance to radiotherapy has been an impediment in the treatment of cancer, and the inability to detect it at an early stage further exacerbates the prognosis. We have assessed the feasibility of Raman spectroscopy as a rapid assay for predicting radiosensitivity of cancer cells in comparison to the conventional biological assays. Cell lines derived from breast adenocarcinoma (MCF7), gingivobuccal squamous cell carcinoma (ITOC-03), and human embryonic kidney (HEK293) were subjected to varying doses of ionizing radiation. Cell viability of irradiated cells was assessed at different time points using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and Raman spectroscopy, and colony-forming capability was evaluated by clonogenic assay. Radiosensitivity observed using MTT assay was limited by the finding of similar cell viability in all the three cell lines 24 h post-irradiation. However, cell survival assessed using clonogenic assay and principal component linear discriminant analysis (PC-LDA) classification of Raman spectra showed correlating patterns. Irradiated cells showed loss of nucleic acid features and enhancement of 750 cm−1 peak probably attributing to resonance Raman band of cytochromes in all three cell lines. PC-LDA analysis affirmed MCF7 to be a radioresistant cell line as compared to ITOC-03 and HEK293 to be the most radiosensitive cell line. Raman spectroscopy is shown to be a rapid and alternative assay for identification of radiosensitivity as compared to the gold standard clonogenic assay.

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CYTOTOXIC ACTIVITY OF LUVUNGA SCANDENS AGAINST HUMAN CANCER CELL LINES

Jurnal Teknologi ◽

10.11113/jt.v78.7328 ◽

2016 ◽

Vol 78 (10) ◽

Author(s):

Putri Nur Hidayah Al-Zikri ◽

Muhammad Taher ◽

Deny Susanti ◽

Solachuddin Jauhari Arief Ichwan

Keyword(s):

Cytotoxic Activity ◽

Cell Line ◽

Cell Lines ◽

A549 Cell ◽

Human Cancer ◽

In Vitro Cytotoxicity ◽

Breast Adenocarcinoma ◽

Human Cancer Cell Lines ◽

Mcf 7

Luvunga scandens belongs to the family of Rutaceae which usually inhabit tropical and moist environment. This plant is known as ‘Mengkurat Jakun’ among locals and used traditionally to treat fever and fatigue via decoction. The aim of this study was to investigate the cytotoxic activity of the leaves and stems extracts of L. scandens extract. Extracts of the leaves and stems were obtained from sequential extraction procedures by various organic solvents. All extracts were subjected to cytotoxic study by 3-(4, 5-dimethylthaizol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. In in vitro cytotoxicity assay, all L. scandens extracts exhibited cytotoxicity against human breast adenocarcinoma (MCF-7) and human lung adenocarcinoma (A549) cell lines. The IC50 values of dichloromethane and methanol extracts from the leaves of L. scandens against MCF-7 cell line were 62.5 µg/mL and 88.0 µg/mL, respectively, whereas IC50 of methanol extract from stem was 81.0 µg/mL. All extracts were less active against A549 cell line where IC50 value were not be determined. The present findings revealed the potential of L. scandens as a cytotoxic agent against MCF-7 cell line. However, further studies should be planned to evaluate role of the plant in cytotoxic activity.

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