scholarly journals Comparison between Tail Suspension Swing Test and Standard Rotation Test in Revealing Early Motor Behavioral Changes and Neurodegeneration in 6-OHDA Hemiparkinsonian Rats

2020 ◽  
Vol 21 (8) ◽  
pp. 2874 ◽  
Author(s):  
Ilaria Rosa ◽  
Davide Di Censo ◽  
Brigida Ranieri ◽  
Giuseppe Di Giovanni ◽  
Eugenio Scarnati ◽  
...  
Keyword(s):  
Neuronal Degeneration ◽  
Behavioral Changes ◽  
Substantia Nigra Pars Compacta ◽  
Motor Asymmetry ◽  
Tail Suspension ◽  
Behavioral Deficits ◽  
Rotation Test ◽  
Post Surgery ◽  
6 Hydroxydopamine ◽  
The Right

The unilateral 6-hydroxydopamine (6-OHDA) model of Parkinson’s disease (PD) is one of the most commonly used in rodents. The anatomical, metabolic, and behavioral changes that occur after severe and stable 6-OHDA lesions have been extensively studied. Here, we investigated whether early motor behavioral deficits can be observed in the first week after the injection of 6-OHDA into the right substantia nigra pars compacta (SNc), and if they were indicative of the severity of the dopaminergic (DAergic) lesion in the SNc and the striatum at different time-points (day 1, 3, 5, 7, 14, 21). With this aim, we used our newly modified tail suspension swing test (TSST), the standard rotation test (RT), and immunohistochemical staining for tyrosine hydroxylase (TH). The TSST, but not the standard RT, revealed a spontaneous motor bias for the 6-OHDA-lesioned rats from the day 1 post-surgery. Both tests detected the motor asymmetry induced by (single and repeated) apomorphine (APO) challenges that correlated, in the first week, with the DAergic neuronal degeneration. The described TSST is fast and easy to perform, and in the drug-free condition is useful for the functional assessment of early motor asymmetry appearing after the 6-OHDA-lesion in the SNc, without the confounding effect of APO challenges.

scholarly journals Neurodegenerative Shielding by Curcumin and Its Derivatives on Brain Lesions Induced by 6-OHDA Model of Parkinson's Disease in Albino Wistar Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Shyam Sunder Agrawal ◽  
Sumeet Gullaiya ◽  
Vishal Dubey ◽  
Varun Singh ◽  
Ashok Kumar ◽  
...  
Keyword(s):  
Binding Assay ◽  
Neuronal Degeneration ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Brain Lesions ◽  
Significant Protection ◽  
6 Hydroxydopamine ◽  
The Right ◽  
Per Oral ◽  
Oxidative Attack

Study was undertaken to evaluate the neurodegenerative defending potential of curcumin (CUR), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) on 6-hydroxydopamine-(6-OHDA) induced Parkinsonism model in rats. Curcuminoids were administered (60 mg/kg, body weight, per oral) for three weeks followed by unilateral injection of 6-OHDA on 22nd day (10 μg/2 μL) into the right striatum leading to extensive loss of dopaminergic cells. The behavioral observations, biochemical markers, quantification of dopamine (DA), DOPAC, and HVA followed by dopamine (D2) receptor binding assay and tyrosine hydroxylase (TH, using immunohistochemistry) were evaluated using HPLC after three weeks of lesion. Pretreated animals showed significant protection against neuronal degeneration compared to lesion animals by normalizing the deranged levels of biomarkers and showed the potency in the order CUR > DMC > BDMC. The same order of effectiveness was observed in D2 receptors binding assay and TH immunohistochemistry study. We conclude that curcuminoids appear to shield progressive neuronal degeneration from increased oxidative attack in 6-OHDA-lesioned rats through its free radical scavenging mechanism, and DA, DOPAC, and HVA enhancing capabilities in the sequence of efficacy CUR > DMC > BDMC. Further, curcuminoids may have potential utility in treatment of many more oxidative stress-induced neurodegenerative disorders.

scholarly journals The effect of nano-hydroxyapatite/chitosan scaffolds on rat calvarial defects for bone regeneration

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Emmanouil Chatzipetros ◽  
Spyros Damaskos ◽  
Konstantinos I. Tosios ◽  
Panos Christopoulos ◽  
Catherine Donta ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Mean Value ◽  
Parietal Bone ◽  
Female Rats ◽  
Sprague Dawley ◽  
Post Surgery ◽  
Group A ◽  
The Mean ◽  
Group B ◽  
The Right

Abstract Background This study aims at determining the biological effect of 75/25 w/w nano-hydroxyapatite/chitosan (nHAp/CS) scaffolds on bone regeneration, in terms of fraction of bone regeneration (FBR), total number of osteocytes (Ost), and osteocyte cell density (CD), as well as its biodegradability. Methods Two critical-size defects (CSDs) were bilaterally trephined in the parietal bone of 36 adult Sprague-Dawley rats (18 males and 18 females); the left remained empty (group A), while the right CSD was filled with nHAp/CS scaffold (group B). Two female rats died postoperatively. Twelve, 11, and 11 rats were euthanized at 2, 4, and 8 weeks post-surgery, respectively. Subsequently, 34 specimens were resected containing both CSDs. Histological and histomorphometric analyses were performed to determine the FBR, calculated as [the sum of areas of newly formed bone in lateral and central regions of interest (ROIs)]/area of the original defect, as well as the Ost and the CD (Ost/mm2) in each ROI of both groups (A and B). Moreover, biodegradability of the nHAp/CS scaffolds was estimated via the surface area of the biomaterial (BmA) in the 2nd, 4th, and 8th week post-surgery. Results The FBR of group B increased significantly from 2nd to 8th week compared to group A (P = 0.009). Both the mean CD and the mean Ost values of group B increased compared to group A (P = 0.004 and P < 0.05 respectively). Moreover, the mean value of BmA decreased from 2nd to 8th week (P = 0.001). Conclusions Based on histological and histomorphometric results, we support that 75/25 w/w nHAp/CS scaffolds provide an effective space for new bone formation.

scholarly journals A stable method for 4D CT-based CFD simulation in the right ventricle of a TGA patient

2020 ◽  
Vol 35 (5) ◽  
pp. 315-324
Author(s):  
Yuri Vassilevski ◽  
Alexander Danilov ◽  
Alexander Lozovskiy ◽  
Maxim Olshanskii ◽  
Victoria Salamatova ◽  
...  
Keyword(s):  
Right Ventricle ◽  
Cfd Simulation ◽  
Fluid Motion ◽  
4D Ct ◽  
Post Surgery ◽  
Surgery Patient ◽  
Time Dependent Domain ◽  
Flow Domain ◽  
The Right ◽  
Dependent Domain

AbstractThe paper discusses a stabilization of a finite element method for the equations of fluid motion in a time-dependent domain. After experimental convergence analysis, the method is applied to simulate a blood flow in the right ventricle of a post-surgery patient with the transposition of the great arteries disorder. The flow domain is reconstructed from a sequence of 4D CT images. The corresponding segmentation and triangulation algorithms are also addressed in brief.

scholarly journals Intraocular Lens Dislocation after Cataract Surgery in Tambolaka, Southwest Sumba, Indonesia: A Case Report

2018 ◽  
Vol 9 (1) ◽  
pp. 179-184
Author(s):  
Ratna Sitompul
Keyword(s):  
Case Report ◽  
Intraocular Lens ◽  
Rural Areas ◽  
Rare Complication ◽  
Cataract Extraction ◽  
Factors Affecting ◽  
Intraocular Lens Dislocation ◽  
Post Surgery ◽  
The Right ◽  
Iol Dislocation

Intraocular lens (IOL) dislocation is a rare complication of cataract extraction requiring prompt surgery. This case report aims to raise awareness of such cases and the importance of post-surgery follow-up. A 58-year-old female patient was found with anterior IOL dislocation a week after phacoemulsification surgery in her right eye. Visual acuity of the right eye was 1/60 with ciliary injection and IOL dislocation to the anterior chamber of the right eye. The patient underwent surgery of the right eye and the IOL haptic was found to be broken. In this case report, the factors affecting IOL dislocation are axis length, broken IOL haptic, and patient activity that increased intraocular pressure. Cataract extraction surgery, although common, needs to be conducted carefully, and it is important for ophthalmologists and general practitioners to detect this condition, especially in rural areas where facilities are limited, as IOL dislocation could occur and requires immediate treatment to achieve a better result.

Stimulation of the nigrostriatal dopamine system produces hypertension and tachycardia in rats

1994 ◽  
Vol 266 (6) ◽  
pp. H2489-H2496 ◽  
Author(s):  
M. T. Lin ◽  
J. J. Yang
Keyword(s):  
Corpus Striatum ◽  
Substantia Nigra Pars Compacta ◽  
Dopamine System ◽  
Arterial Blood ◽  
Nigrostriatal Dopamine ◽  
Induced Hypertension ◽  
Bilateral Vagotomy ◽  
6 Hydroxydopamine ◽  
Da Release ◽  
Stimulation Of

To test for the ability of the nigrostriatal dopamine (DA) system to influence cardiovascular function, experiments were carried out to assess the effects of electrical or chemical stimulation of the nigrostriatal DA system on arterial blood pressure, heart rate, and striatal DA release in anesthetized rats. Electrical stimulation of the substantia nigra pars compacta (SNC), in addition to enhancing the DA release in the corpus striatum (CS), elicited proportional hypertension and tachycardia. This could be mimicked by microinjection of two excitatory amino acids, kainic acid and glutamate, into the SNC area of rat brain. The SNC stimulation-induced hypertension, tachycardia, and increased striatal DA release were attenuated by prior destruction of the nigrostriatal DA system produced by intramedial forebrain bundle injection of 6-hydroxydopamine and by prior blockade of postsynaptic DA receptors produced by intra-CS injection of DA receptor antagonists, haloperidol or pimozide. The SNC stimulation-induced hypertension was attenuated by spinal transection, whereas the SNC stimulation-induced tachycardia was attenuated by bilateral vagotomy. The data suggest that stimulation of the nigrostriatal DA system produces both hypertension and tachycardia in rats.

scholarly journals Key Mechanisms and Potential Implications of Hericium erinaceus in NLRP3 Inflammasome Activation by Reactive Oxygen Species during Alzheimer’s Disease

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1664
Author(s):  
Marika Cordaro ◽  
Angela Trovato Salinaro ◽  
Rosalba Siracusa ◽  
Ramona D’Amico ◽  
Daniela Impellizzeri ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Nlrp3 Inflammasome ◽  
Neuronal Degeneration ◽  
Behavioral Changes ◽  
Antioxidant Systems ◽  
Inflammasome Activation ◽  
Protective Effects ◽  
Behavioral Alteration ◽  
Hericium Erinaceus

Alzheimer’s disease (AD) is the principal cause of dementia, and its incidence increases with age. Altered antioxidant systems and inflammation have an important role in the etiology of neurodegenerative disorders. In this study, we evaluated the effects of Hericium erinaceus, a nutritional mushroom with important antioxidant effects, in a rat model of AD. Animals were injected with 70 mg/Kg of AlCl3 daily for 6 weeks, and Hericium erinaceus was administered daily by gavage. Before the experiment’s end date, behavioral test training was performed. At the end of the study, behavioral changes were assessed, and the animals were euthanized. Brain tissues were harvested for further analysis. AlCl3 mainly accumulates in the hippocampus, the principal region of the brain involved in memory functions and learning. Hericium erinaceus administration reduced behavioral changes and hippocampal neuronal degeneration. Additionally, it reduced phosphorylated Tau levels, aberrant APP overexpression, and β-amyloid accumulation. Moreover, Hericium erinaceus decreased the pro-oxidative and pro-inflammatory hippocampal alterations induced by AD. In particular, it reduced the activation of the NLRP3 inflammasome components, usually activated by increased oxidative stress during AD. Collectively, our results showed that Hericium erinaceus has protective effects on behavioral alteration and histological modification associated with AD due to the modulation of the oxidative and inflammatory pathways, as well as regulating cellular brain stress.

scholarly journals Experience Summary of Laparoscopic Treatment for Pediatric Ureteral Polyps

2021 ◽  
Vol 9 ◽  
Author(s):  
Hongjie Gao ◽  
Jiawei Chen ◽  
Guowei Li ◽  
Xinhai Cui ◽  
Fengyin Sun
Keyword(s):  
Surgical Techniques ◽  
Kidney Size ◽  
Intraoperative Bleeding ◽  
Minimally Invasive Surgical ◽  
Post Surgery ◽  
Diseased Segment ◽  
The Right ◽  
Segmental Ureterectomy ◽  
Ureteral Polyps

Objective: To investigate surgical techniques and challenges of laparoscopic in treating pediatric ureteral polyps under laparoscopy.Methods: The clinical data of 7 of pediatric ureteral polyps patients who were admitted to the hospital from July 2015 to January 2020 were analyzed retrospectively. There were 6 males and 1 female from 7.7 to 13.9 years old at the mean age of 10.4. Before surgery, all children performed urinary B ultrasound, magnetic resonance urography (MRU), and renal radionuclide scanning. Six cases were observed on the left lateral and 1 on the right. The lesions of 5 cases were located at the ureteropelvic junction, 1 in the upper ureter and 1 in the middle ureter. The polyps were treated intraoperatively by the resecting of the lesion segment and simple polypectomy to retain the attached part of the original diseased segment of the ureter. All surgeries were performed under laparoscopy and B-ultrasound was performed during follow up after surgery.Results: All 7 surgeries were performed successfully under the laparoscope. The surgery time was 80–110 min, and the average surgery time was 97.5 min. The intraoperative bleeding was 10–25 ml and the average postoperative hospital stay was 6 d. Postoperative hematuria occurred in 1 case. Neither urinary leakage nor urinary tract infection was reported post surgery. Preoperative affected pyelectasis of all patients was 2.0–3.7 cm. Three months postoperatively, the affected pyelectasis was measured at 1.2–3.0 cm. No recurrence of polyps was reported after surgery. During the follow-up to April 2020, there was no significant change in the kidney size of all patients, and hydronephrosis was alleviated compared with that before surgery.Conclusions: Laparoscopy is a safe, effective and minimally invasive surgical technique for pediatric multiple ureteral polyps. The surgery plan was designed according to the location and size of polyps, including segmental ureterectomy of polyps + pyeloureterostomy, segmental ureterectomy of polyps + ureter - ureteral anastomosis.

scholarly journals Giant dentigerous cyst of maxillary sinus associated with impacted maxillary canine

2019 ◽  
Vol 7 (10) ◽  
pp. 3901
Author(s):  
P. P. G. Krisna Bayu Pramana Rimbawan ◽  
I. Putu Gede Windhu Saputra ◽  
Ratna Rayeni Natasha Roosseno
Keyword(s):  
Histopathological Examination ◽  
Dentigerous Cyst ◽  
Facial Asymmetry ◽  
Aesthetic Surgery ◽  
Maxillary Canine ◽  
General Physician ◽  
Post Surgery ◽  
Stratified Squamous Epithelium ◽  
Ectopic Tooth ◽  
The Right

Dentigerous cyst is a cyst that encapsulates enamel of the unerupted tooth, thus causing the tooth become unable to complete the eruption process. This cyst is defined as a developmental cyst as it generally occurs in first until the third decades of life. Dentigerous cyst commonly affect the mandibular area of the teeth. This article describes the case of a young patient with dentigerous cyst which affect maxillary canine. A 6-year-old girl was admitted to Plastic Reconstructive and Aesthetic Surgery Clinic at Mangusada Hospital Bali with facial asymmetry on her right cheek, noticed since the past one week. She got some medicines from the general physician, but there was no improvement. From the CT scan, we found a solitary mass on the size of 3 x 3.2 x 3.6 cm on the right maxillary area. Enucleation was done and the Caldwell-Luc approach was performed under general anesthesia. The cyst revealed to have an ectopic maxillary canine inside. The cyst and ectopic tooth were extracted from the pathological site and the incisional specimens were sent for histopathological examination. The result revealed a cyst lined by non-keratinized stratified squamous epithelium without any malignancy. She had antibiotics therapy post-surgery, and her symptoms were resolved within a week. In one month follow up, there was no sign of recurrence.

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