scholarly journals Antimicrobial and Amyloidogenic Activity of Peptides Synthesized on the Basis of the Ribosomal S1 Protein from Thermus Thermophilus

2020 ◽  
Vol 21 (17) ◽  
pp. 6382 ◽  
Author(s):  
Stanislav Kurpe ◽  
Sergei Grishin ◽  
Alexey Surin ◽  
Olga Selivanova ◽  
Roman Fadeev ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Thermus Thermophilus ◽  
Antibacterial Properties ◽  
Cell Penetration ◽  
Research Directions ◽  
Bacterial Proteins ◽  
Hiv Transcription ◽  
Parent Protein ◽  
Amyloidogenic Peptides ◽  
New Research

Controlling the aggregation of vital bacterial proteins could be one of the new research directions and form the basis for the search and development of antibacterial drugs with targeted action. Such approach may be considered as an alternative one to antibiotics. Amyloidogenic regions can, like antibacterial peptides, interact with the “parent” protein, for example, ribosomal S1 protein (specific only for bacteria), and interfere with its functioning. The aim of the work was to search for peptides based on the ribosomal S1 protein from T. thermophilus, exhibiting both aggregation and antibacterial properties. The biological system of the response of Gram-negative bacteria T. thermophilus to the action of peptides was characterized. Among the seven studied peptides, designed based on the S1 protein sequence, the R23I (modified by the addition of HIV transcription factor fragment for bacterial cell penetration), R23T (modified), and V10I (unmodified) peptides have biological activity that inhibits the growth of T. thermophilus cells, that is, they have antimicrobial activity. But, only the R23I peptide had the most pronounced activity comparable with the commercial antibiotics. We have compared the proteome of peptide-treated and intact T. thermophilus cells. These important data indicate a decrease in the level of energy metabolism and anabolic processes, including the processes of biosynthesis of proteins and nucleic acids. Under the action of 20 and 50 μg/mL R23I, a decrease in the number of proteins in T. thermophilus cells was observed and S1 ribosomal protein was absent. The obtained results are important for understanding the mechanism of amyloidogenic peptides with antimicrobial activity and can be used to develop new and improved analogues.

INNOVATION CAPABILITY, ABSORPTIVE CAPACITY AND SMES PERFORMANCE IN PAKISTAN: THE MODERATING EFFECT OF BUSINESS STRATEGY

2018 ◽  
Vol 13 (Number 2) ◽  
pp. 1-11
Author(s):  
Muhammad Zulqarnain Arshad ◽  
Darwina Arshad
Keyword(s):  
Economic Growth ◽  
Absorptive Capacity ◽  
Business Strategy ◽  
Innovation Capability ◽  
Research Directions ◽  
Conceptual Paper ◽  
And Performance ◽  
New Research ◽  
The Relationship ◽  
Crucial Part

The small and medium-sized enterprises (SMEs) play a crucial part in county’s economic growth and a key contributor in country’s GDP. In Pakistan SMEs hold about 90 percent of the total businesses. The performance of SMEs depends upon many factors. The main aim for the research is to examine the relationship between Innovation Capability, Absorptive Capacity and Performance of SMEs in Pakistan. This conceptual paper also extends to the vague revelation on Business Strategy in which act as a moderator between Innovation Capability, Absorptive Capacity and SMEs Performance. Conclusively, this study proposes a new research directions and hypotheses development to examine the relationship among the variables in Pakistan’s SMEs context.

MATERIAL HANDLING MACHINES AND SYSTEMS – UMI-TWINN PROJECT CONTRIBUTION

2019 ◽  
Vol 12 (1) ◽  
pp. 7-20
Author(s):  
Péter Telek ◽  
Béla Illés ◽  
Christian Landschützer ◽  
Fabian Schenk ◽  
Flavien Massi
Keyword(s):  
Material Handling ◽  
Digital Data ◽  
Flow Process ◽  
Research Directions ◽  
Scientific Excellence ◽  
Research Activities ◽  
New Research ◽  
Eu Project ◽  
The University

Nowadays, the Industry 4.0 concept affects every area of the industrial, economic, social and personal sectors. The most significant changings are the automation and the digitalization. This is also true for the material handling processes, where the handling systems use more and more automated machines; planning, operation and optimization of different logistic processes are based on many digital data collected from the material flow process. However, new methods and devices require new solutions which define new research directions. In this paper we describe the state of the art of the material handling researches and draw the role of the UMi-TWINN partner institutes in these fields. As a result of this H2020 EU project, scientific excellence of the University of Miskolc can be increased and new research activities will be started.

scholarly journals Edible Films from Carrageenan/Orange Essential Oil/Trehalose—Structure, Optical Properties, and Antimicrobial Activity

Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 332
Author(s):  
Jancikova Simona ◽  
Dordevic Dani ◽  
Sedlacek Petr ◽  
Nejezchlebova Marcela ◽  
Treml Jakub ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antibacterial Properties ◽  
Edible Films ◽  
Attenuated Total Reflectance ◽  
Practical Application ◽  
Film Properties ◽  
Gram Positive Bacteria ◽  
Edible Packaging ◽  
Orange Essential Oil

The research aim was to use orange essential oil and trehalose in a carrageenan matrix to form edible packaging. The edible packaging experimentally produced by casting from an aqueous solution were evaluated by the following analysis: UV-Vis spectrum, transparency value, transmittance, attenuated total reflectance Fourier-Transform spectroscopy (FTIR), scanning electron microscopy (SEM) and antimicrobial activity. The obtained results showed that the combination of orange essential oil with trehalose decreases the transmittance value in the UV and Vis regions (up to 0.14% ± 0.02% at 356 nm), meaning that produced films can act as a UV protector. Most produced films in the research were resistant to Gram-positive bacteria (Staphylococcus aureus subsp. aureus), though most films did not show antibacterial properties against Gram-negative bacteria and yeasts. FTIR and SEM confirmed that both the amount of carrageenan used and the combination with orange essential oil influenced the compatibility of trehalose with the film matrix. The research showed how different combinations of trehalose, orange essential oils and carrageenan can affect edible film properties. These changes represent important information for further research and the possible practical application of these edible matrices.

scholarly journals A Study on the Performance of Unconstrained Very Low Resolution Face Recognition: Analyzing Current Trends and New Research Directions

IEEE Access ◽  
2021 ◽  
pp. 1-1
Author(s):  
Luis S. Luevano ◽  
Leonardo Chang ◽  
Heydi Mendez-Vazquez ◽  
Yoanna Martinez-Diaz ◽  
Miguel Gonzalez-Mendoza
Keyword(s):  
Face Recognition ◽  
Low Resolution ◽  
Research Directions ◽  
Current Trends ◽  
New Research

The Role of Perceived Risk in Engaging Customer and Employees for Value Creation in Services

2021 ◽  
pp. 097226292110225
Author(s):  
Ritu Srivastava ◽  
Diptiman Banerji ◽  
Priyanka Nema ◽  
Shubham Choudhary
Keyword(s):  
Employee Engagement ◽  
Value Creation ◽  
Perceived Risk ◽  
Organizational Outcomes ◽  
Customer Engagement ◽  
Future Research ◽  
Second Phase ◽  
Research Directions ◽  
Marketing Effectiveness ◽  
New Research

Value creation, customer engagement and employee engagement have emerged as important organizational outcomes for continued success. At the turn of the new decade, it is imperative to identify new research directions for these outcomes to improve the marketing effectiveness of organizations while keeping people at the centre of this pursuit. The present study is propelled by this motivation. The study started with the exploration of the relationship of customer and employee engagement in value creation, while limiting the scope to services. The extant literature has not studied the three together. The second phase of the study dwelled on identifying common links among the three to develop a conceptual model that brought the concepts of customer engagement, employee engagement and value creation together. Perceived risk was identified as the underlying phenomenon that connected all three to be part of a social system. A conceptual framework has been proposed for connecting perceived risk to customer engagement and employee engagement that would create value in service organizations. The study identifies future research directions for theory building and practice.

Survey of Uses of Evolutionary Computation Algorithms and Swarm Intelligence for Network Intrusion Detection

2015 ◽  
Vol 14 (04) ◽  
pp. 1550025 ◽  
Author(s):  
Saber Elsayed ◽  
Ruhul Sarker ◽  
Daryl Essam
Keyword(s):  
Intrusion Detection ◽  
Swarm Intelligence ◽  
Computational Intelligence ◽  
Network Intrusion Detection ◽  
The Internet ◽  
Research Directions ◽  
Related Literature ◽  
Network Intrusion ◽  
Computation Algorithms ◽  
New Research

Many infrastructures, such as those of finance and banking, transportation, military and telecommunications, are highly dependent on the Internet. However, as the Internet’s underlying structural protocols and governance can be disturbed by intruders, for its smooth operation, it is important to minimize such disturbances. Of the available techniques for achieving this, computational intelligence methodologies, such as evolutionary algorithms and swarm intelligence approaches, are popular and have been successfully applied to detect intrusions. In this paper, we present an overview of these techniques and related literature on intrusion detection, analyze their research contributions, compare their approaches and discuss new research directions which will provide useful insights for intrusion detection researchers and practitioners.

Reflections on the biochemistry ofEchinococcus: past, present and future

Parasitology ◽  
2009 ◽  
Vol 136 (12) ◽  
pp. 1643-1652 ◽  
Author(s):  
D. P. McMANUS
Keyword(s):  
Hydatid Disease ◽  
Biochemical Analysis ◽  
Species Variation ◽  
Research Directions ◽  
Future Studies ◽  
Tegumental Surface ◽  
Research Areas ◽  
General Metabolism ◽  
New Research ◽  
The Impact

SUMMARYThis review discusses 5 of my earliest papers on the biochemistry of larvalEchinococcuspublished inParasitologyin the 1970s and 1980s. Two of the publications consider aspects of the basic biochemistry, intermediary metabolism and the regulation of respiratory pathways inE. granulosusandE. multilocularis, and emphasize the existence of inter- and intra-species variation in their general metabolism. The third reports on the detailed biochemical analysis of the tegumental surface of the protoscolex ofE. granulosus, and the final 2 papers describe the genomic cloning ofEchinococcusDNA fragments and their use, along with other DNA markers, in molecular identification ofE. granulosusisolates collected worldwide from areas endemic for hydatid disease. A number of years have elapsed since these publications inParasitologyand, in this Centenary Issue article, I reflect briefly on some of the subsequent studies undertaken in these research areas that have advanced the field. As well, I provide brief insight on new research directions, emphasizing the impact of molecular biology and associated techniques on future studies ofEchinococcusand hydatid disease.

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