Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis Media

Recurrent acute otitis media (RAOM) in children is clinically defined as the occurrence of at least three episodes of acute otitis media over a course of 6 months. A further common pathological condition of interest in the context of pediatric otolaryngology is adenotonsillar hypertrophy (ATH), a common cause of obstructive sleep apnea syndrome. Aimed at unraveling the differential modulation of proteins in the two pathologies and at understanding the possible pathways involved in their onset, we analyzed the proteomic profile of the adenoids from 14 RAOM and ATH patients by using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). The 2-DE coupled with MS allowed us to identify 23 spots with significant (p-value < 0.05) changes in protein amount, recognizing proteins involved in neutrophil degranulation and glycolysis pathways.

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Microbiological Profile of Adenoid Hypertrophy Correlates to Clinical Diagnosis in Children

BioMed Research International ◽

10.1155/2013/629607 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 9

Author(s):

Anita Szalmás ◽

Zoltán Papp ◽

Péter Csomor ◽

József Kónya ◽

István Sziklai ◽

...

Keyword(s):

Otitis Media ◽

Clinical Characteristics ◽

Sleep Apnea Syndrome ◽

Acute Otitis Media ◽

Respiratory Viruses ◽

Bacterial Biofilm ◽

Bacterial Biofilms ◽

Human Bocavirus ◽

Adenoid Hypertrophy ◽

Obstructive Sleep

Objective. Adenoid hypertrophy is a common condition in childhood, which may be associated with recurring acute otitis media (RAOM), otitis media with effusion (OME), and obstructive sleep apnea syndrome (OSAS). These different clinical characteristics have some clinical overlap; however, they might be explained by distinct immunologic and infectious profiles and result in various histopathologic findings of adenoid specimens.Methods. A total of 59 children with adenoid hypertrophy undergoing adenoidectomy were studied. Three series of identical adenoid specimens were processed to hematoxylin-eosin (H.E.) and Gram staining and to respiratory virus specific real-time PCR, respectively.Results. According to the clinical characteristics, patients were recruited into three groups: RAOM (). Bacterial biofilms were detected in 21 cases, while at least one of the studied respiratory viruses was detected in 52 specimens. RAOM cases were significantly associated with biofilm existence (). In contrast, OME group was characterized by the absence of bacterial biofilm and by normal mucosa. Showing a statistically significant correlation, all OME cases were positive for human bocavirus (HBoV, ).Conclusions. Bacterial biofilms might contribute to the damage of respiratory epithelium and recurring acute infections resulting in RAOM. In OME cases persisting respiratory viruses, mainly HBoV, can cause subsequent lymphoid hyperplasia leading to ventilation disorders and impaired immunoreactivity of the middle ear cleft.

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Evaluation of Antifungal Activity of CalvatiaCraniiformis and IverMectin as Novel Alternative Therapies for Aspergillus Niger Associated Acute Otitis Media with Special Refer to SocioDemographic Factors Among Rural Children of Diyala Province-Iraq

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i08.9583 ◽

2017 ◽

Vol 9 (08) ◽

Cited By ~ 1

Author(s):

Ghassan H. Jameel ◽

Ali Ibrahim Ali Al-Ezzy

Keyword(s):

Antifungal Activity ◽

Aspergillus Niger ◽

Otitis Media ◽

Family Size ◽

Acute Otitis Media ◽

Sociodemographic Factors ◽

P Value ◽

Rural Children ◽

Alcohol Extract ◽

Family Economy

Objectives are to determine antifungal activity of Ivermectin and Calvatiacraniiformis as a novel alternative therapy for aspergillus niger associated acute otitis media (AOM) among rural children of Diyala province; correlation of sociodemographic factors with frequency of infection. Ear swabs taken from 58 infected children and cultured onSabouraud dextrose agar for 7-14 days .Macroscopic and microscopic criteria used for diagnosis of A.niger .High isolation rate for A.niger (27.59%) among children of (4-6) years with significant difference between age groups ( p value 0.039); genders ( p value 0.004);house status(p value=0.018);family size (p value =0.00006334) and month of infection (p value=0.000). A.niger infection negatively correlated with patients age (p value =0.039), family economy and house status (p value =0.000),family size (p value =0.000). Alcohol extract of C.craniiformis (100mg, 200mg, 400mg, 500mg, 600mg, 800mg and 1000 mg) and ivermectin (0.5%,1 % and 2%) restricted the growth of A. niger after 3 days .Significance difference reported between all concentrations except 100 mg and 200 mg ; 600 mg and 800 mg. Significance difference in inhibitory activity between concentration 1% and 2%,0.5% and 2% of Ivermectin respectively. Conclusions: A.niger infections positively correlated with family size and inversely with age and family economy. The growth of A nigersignificantly restricted by alcohol extract of C.craniiformis and Ivermectin in concentration dependent manner. The powerful concentration was 1000mg, for C.craniiformis and 2% for Ivermectin. Thus, C.craniiformis and Ivermectin consider a novel antifungal agents that can be used in clinical practice for treatment of A.niger associated otitis media that represents a clinical problem in children and need serious attention from clinicians.

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Home testing for pediatric obstructive sleep apnea syndrome secondary to adenotonsillar hypertrophy

Pediatric Pulmonology ◽

10.1002/ppul.1950200407 ◽

1995 ◽

Vol 20 (4) ◽

pp. 241-252 ◽

Cited By ~ 94

Author(s):

S. V. Jacob ◽

A. Morielli ◽

M. A. Mograss ◽

F. M. Ducharme ◽

M. D. Schloss ◽

...

Keyword(s):

Obstructive Sleep Apnea ◽

Sleep Apnea ◽

Obstructive Sleep Apnea Syndrome ◽

Sleep Apnea Syndrome ◽

Adenotonsillar Hypertrophy ◽

Home Testing ◽

Obstructive Sleep ◽

Apnea Syndrome

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Effectiveness of Tympanic Thermometry for diagnosing Acute Otitis Media

Journal of Islamabad Medical & Dental College ◽

10.35787/jimdc.v8i2.359 ◽

2019 ◽

Vol 8 (2) ◽

pp. 74-78

Author(s):

Muhammad Zubair ◽

Ghulam Saqulain ◽

Arfat Jawaid

Keyword(s):

Tympanic Membrane ◽

Otitis Media ◽

Temperature Difference ◽

Acute Otitis Media ◽

P Value ◽

Upper Respiratory Tract ◽

Mean Temperature ◽

Ear Wax ◽

The Mean ◽

Group B

Background: Acute Otitis Media (AOM) is a common upper respiratory tract infection (URTI) in children and usually presents with fever and otalgia. AOM is characterized by congested tympanic membrane and possible increase in temperature, which might be picked up by infrared tympanic thermometry. The objective of this study was to compare the temperature difference of tympanic membrane of affected ear with the unaffected ear and axilla in unilateral acute otitis media, and compare it with the control group.Material and Methods: This case control study comprised of 200 cases of both genders, aged up to 5 years. They were divided into two groups; Group A included 100 clinically diagnosed cases of acute otitis media (AOM), who reported in the ENT Outpatient Department (OPD) and Group B included 100 controls who presented in General Filter Clinic with no ear complaints. Cases with chronic ear disease, ear discharge, and use of local drugs including ear drops, impacted ear wax, tragal tenderness and congenital malformations of the ear were excluded by taking a detailed history. Clinical examination including otoscopy by an expert was done before subjecting patients to axillary and tympanic thermometry measurements and data recording. Data was collected and tabulated using Microsoft Excel Worksheet and analyzed by SPSS 16. Qualitative data like gender were presented as percentage and ratio, while means and standard deviation were calculated for the quantitative data. Difference between the means of experimental and control groups were analyzed by independent sample t-test and P value of less than or equal to 0.05 was taken as significant.Results: This study included 100 cases of unilateral AOM and 100 normal controls without AOM. In patients with AOM, the mean temperature difference between the affected ear and axilla was 1.41ºF as compared to 0.075ºF in controls (p=0.026). While the mean temperature difference between the affected ear and other ear was 0.65ºF as compared to 0.19ºF in controls (p=0.069).Conclusion: In acute otitis media, the temperature of affected ear is significantly higher than axilla but was not significantly higher than the other ear. The finding may help establish thermometry as a diagnostic tool in clinics manned by doctors not competent to do otoscopy.

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Hearing loss in mild OSAS and simple snoring patients

Otolaryngologia Polska ◽

10.5604/01.3001.0009.8409 ◽

2017 ◽

Vol 71 (2) ◽

pp. 11-15

Author(s):

Maria Carla Spinosi ◽

Francesca D'Amico ◽

Giulio Passali ◽

Cemal Cingi ◽

Hugo Rodriguez ◽

...

Keyword(s):

Hearing Loss ◽

Pathological Condition ◽

Sleep Apnea Syndrome ◽

Study Groups ◽

Control Group ◽

Auditory Function ◽

Upper Airways ◽

Obstructive Sleep ◽

The Impact ◽

Simple Snoring

Introduction Obstructive Sleep Apnea Syndrome (OSAS) is characterized by recurrent episodes of prolonged partial or complete obstruction of the upper airways. Several study groups studied the effect of snoring and OSAS on auditory function, showing an increase in the incidence of hearing loss in apneic patients, an earlier onset and/or a degree of hearing loss deeper than in peers. The aim of our study is to evaluate the audiological performance of a population of simple-snoring patients and patients suffering from mild OSAS, and the impact that such pathological condition can have on the auditory function, considering the significant levels of chronic noise. Materials and Methods Data was collected by analyzing audiometric exams on snoring patients and a control group of non-snoring patients. Our study included simple snoring patients without OSAS (AHI < 5) or with low level of OSAS (5< AHI < 15). Possible hearing loss was classified in a crescent scale (A-B-C-D-E), from greater to minor auditory performance, according to the indications of the national protocol of occupational medicine for evaluation of hearing loss in patients exposed to chronic noise. Results We have found independence between simple snorers, mild-OSAS snorers and non-snoring patients compared to the scale of hearing performance classification. Conclusions Our results show a distribution of hearing loss in the different groups that appear independent of the presence or absence of snoring, complicated or uncomplicated by mild OSAS.

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Hubungan Rinitis Alergi Dengan Otitis Media Akut Pada Anak Di RSUD Dr. H Abdul Moeloek Bandar Lampung Tahun

MAHESA : Malahayati Health Student Journal ◽

10.33024/mahesa.v1i3.3924 ◽

2021 ◽

Vol 1 (3) ◽

pp. 198-203

Author(s):

Muslim Kasim ◽

Arti Febriyani Hutasuhut ◽

Tan’im Arief ◽

Farah Ulya Suryadana

Keyword(s):

Allergic Rhinitis ◽

Data Analysis ◽

Otitis Media ◽

Research Design ◽

Middle Ear ◽

Acute Otitis Media ◽

Sampling Technique ◽

P Value ◽

Chi Square ◽

Cross Sectional

ABSTRACT: RELATIONSHIP BETWEEN ALLERGIC RHINITIS AND ACUTE OTITIS MEDIA IN CHILDREN IN RSUD DR. H ABDUL MOELOEK BANDAR LAMPUNG Introduction: Background : Acute Otitis Media (AOM) is an inflammation that happen in the middle ear and is very common, especially in children. Children are susceptible to acute otitis media (AOM) due to the shorter and horizontal anatomy of the eustachian tube. Allergic Rhinitis is one of the risk factos that caused acute otitis media in children.Purpose : The purpose of this study was to determine the correlation between Allergic Rhinitis and Acute Otitis Media in ChildrenMethod: This study used an observational analytic research with cross sectional research design. The sample in this study was 59 patients diagnosed with acute otitis media at RSUD Dr. H. Abdul Moeloek in 2019-2020. The sampling technique used total sampling. Data analysis using Chi-Square testResult: The results of this study indicated that there was a relationship between allergic rhinitis and acute otitis media in children with p value = 0,047(<0,05) with an OR = 0,146 (95%CI 0,17-1,232). Conclusion: There is a relationship between Allergic Rhinitis and Acute Otitis Media in children at Dr. H Abdul Moeloek Bandar Lampung in 2019-2020 Keyword : Allergic Rhinitis, Acute Otitis Media, Children INTISARI: HUBUNGAN RINITIS ALERGI DENGAN OTITIS MEDIA AKUT PADA ANAK DI RSUD DR. H ABDUL MOELOEK BANDAR LAMPUNG Latar belakang: Otitis Media Akut (OMA) merupakan peradangan yang terjadi pada telinga bagian tengah dan sangat sering terjadi terutama pada anak-anak. Anak-anak rentan terkena Otitis Media Akut (OMA) dikarenakan bentuk anatomi tuba eustachius yang lebih pendek dan horizontal. Rinitis Alergi merupakan salah satu faktor risiko yang menyebabkan Otitis Media Akut pada anak.Tujuan: Tujuan penelitian ini adalah untuk mengetahui hubungan Rinitis Alergi dengan Otitis Media akut pada Anak.Metode: Penelitian analitik observasional dengan desain penelitian cross sectional. Sampel sebanyak 59 pasien dengan diagnosa otitis media akut anak di RSUD Dr. H. Abdul Moeloek tahun 2018. Teknik pengambilan sample menggunakan total sampling. Analisis data menggunakan uji Chi-SquareHasil : Hasil penelitian ini menunjukkan bahwa terdapat hubungan antara rinitis alergi dengan otitis media akut pada anak dengan p value = 0,047(<0,05) dengan nilai OR = 0,146 (95%CI 0,17-1,232).Kesimpulan : Terdapat hubungan antara Rinitis Alergi dengan Otitis Media Akut pada anak di RSUD Dr. H Abdul Moeloek Bandar Lampung tahun 2019-2020 Kata Kunci : Rinitis Alergi, Otitis Media Akut, Anak

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Prediction of Optimal CPAP Pressure in Patients with OSAS in Turkish Population

International Journal Of Medical Science And Clinical Invention ◽

10.18535/ijmsci/v7i01.01 ◽

2020 ◽

Vol 7 (01) ◽

pp. 4675-4680

Author(s):

Hakan Celikhisar ◽

Gulay Dasdemir Ilkhan

Keyword(s):

Positive Airway Pressure ◽

Sleep Apnea Syndrome ◽

Turkish Population ◽

High Accuracy ◽

P Value ◽

Prediction Formula ◽

Obstructive Sleep ◽

Significant Difference ◽

The Mean ◽

New Formula

The most effective therapy defined for the patients with obstructive sleep apnea syndrome (OSAS) is the continuous positive airway pressure (CPAP). In this study, we aimed to construct a CPAP prediction formula in Turkish population in order to decrease the requirements for polysomnography (PSG) laboratories. The demographic features, age, gender, weight and height of the participants were recorded at the first visit, together with the neck, waist and hip circumferences. Epworth Sleepiness Scale (ESS) was used to evaluate the levels of daytime sleepiness. All patients underwent at least two PSG studies. In a total of 269 patients with a mean age of 53.42 ±13.32 years were included in the study. In step-wise multiple regression analysis; BMI, AHI, ARI, and neck circumference were the independent predictors of CPAP. A new formula was developed using these values: CPAP pred: (BMI X 0.138) + (AHI X 0.044) + (ARI X 0.056) +(NECK X 0.151) -6.180. The mean CPAP was 7.70 ±2.48 and the mean CPAP pred was 7.71 ±1.53. When the mean CPAP and CPAP pred values were compared, there was not a significant difference with a p value of 0.846. There was a significant correlation between CPAP and CPAP pred values (p:0.001 and r:0.613). We determined that, predicting CPAP with high accuracy may be possible with formulations. These formulations are important in order to reduce the requirements for repeated PSG. Further, larger, prospective studies are required to increase the accuracy of CPAP predicting formulas.

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Polysonographic changes in obese patients with indication of bariatric surgery

Revista do Colégio Brasileiro de Cirurgiões ◽

10.1590/0100-6991e-20213030 ◽

2021 ◽

Vol 48 ◽

Author(s):

VICTOR MARTINS FERNANDES ◽

GIBRAN RIBEIRO DA ROCHA ◽

THIAGO CARVALHO MILET ◽

DANIEL MATOS BARRETO ◽

JORGE FARIA DE MIRANDA SANTOS ◽

...

Keyword(s):

Bariatric Surgery ◽

Sleep Apnea Syndrome ◽

Cross Sectional Study ◽

Standard Test ◽

P Value ◽

Morbidly Obese ◽

Obese Patients ◽

Cross Sectional ◽

Obstructive Sleep ◽

Respiratory Event

ABSTRACT Introduction: obstructive Sleep Apnea Syndrome (OSAS) is a serious confition that compromises the quality of life and survival of patients. Its main risk fator in adults is obesity and the gold standard test for diagnosis is polysomnography (PSG), mainly through the apneia-hypopnea index (AHI). Objective: to analyze the sleep pattern of obese patients with indication for bariatric surgery, determining the main polisomnographic parameters compromised by obesity. Methods: This work is a cross-sectional study with analysis of polysomnography perfomed in patients with obesity in the peroperative period of bariatric surgery at a clinic in Vitória da Conquista/BA during 2017. The Epi Info 7 platform was used for analysis of the data. Results: 58 polysomnographic reports were analyzed, with 56,9% morbdly obese and 43,1% non-morbid. The prevalence of OSAS was 70,68% and de AHI ranged from zero to 84,6 with a mean of 19,47±22,89 e/h. morbidly obese, compared to “non-morbid”, had a longer saturation time below 80% and 90% (0,4±0,93 vs. 0,12±0,45 e 4,87±7,38 vs. 1,36±2,87 respectively; p-value=0,02 in both), worse index respiratory disorders ((29,24±25,36 vs. 16,88±16,21; p-value=0,02), higher AHI (24,71±25,68 vs. 12,56±16,67; p-value=0,02), higher hypopnea index values (16,41±17,10 vs. 6,99±8,52; p-value=0,006) and lower minimum saturation (78,24±9,80 vs. 85,24±6,33; p-value=0,004). Conclusions: the high prevalence of OSAS found confirms its indication in the preoperative period of bariatric surgery. The main respiratory event involved in most individuals with OSAS was the hypopnea index.

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Features of lesion of ENT-organs in children with Down syndrome

Modern pediatrics. Ukraine ◽

10.15574/sp.2020.109.15 ◽

2020 ◽

pp. 15-18

Author(s):

A.L. Kosakovskyi ◽

◽

Iu. Gavrylenko ◽

M. Kyianytsia ◽

◽

...

Keyword(s):

Down Syndrome ◽

Otitis Media ◽

Primary Care Physicians ◽

Significant Proportion ◽

Acute Otitis Media ◽

Educational Work ◽

Children With Down Syndrome ◽

Obstructive Sleep ◽

Hearing Test ◽

Families With Children

Objective: сlinical relevance and prevalence of ENT diseases in children with Down syndrome (DS) of Ukrainian, according to a survey of their parents. Analyze educational work among primary care physicians. Materials and methods. During 2019, 137 respondents, who were parents of children with Down syndrome, were surveyed. Аge varied from 3 months to 18 years. Results. Scheduled medical examinations in ENT doctors have the ability to conduct 81% of families with children with DS. 72.3% of the interviewed had their hearing assessed, of whom 19% had their hearing checked annually. 27.7% of children with DS do not have a hearing test. A significant proportion (81%) of parents of children with DS did not offer to assess the child's hearing. Acute otitis media is more commonly recorded at the age of 1 to 5 years. Surgical treatment for otitis media was performed in 7.2% of patients (tympano puncture/tympanocentesisin 5.1%; performed more than 2 times in 0.7%; middle ear bypass grafting in 1.5%). Acute bacterial rhinosinusitis was noted in 40.9% of children with DS, of which 82.1% had more than 3 episodes of acute rhinosinusitis during their lifetime. Allergies were reported in 34.3%. Obstructive sleep apnea is noted by 26.3%. 70.8% of parents observe their baby's shortness of breath, snoring. As for surgery: adenotomy was performed in 20.5%, of which 1.5% of children performed more than 1 time. Adenotomy is planned in 30% of the respondents. Tonsillectomy was performed in 1.5%, with 0.7% planned for the near future. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of participating institution. The informed consent of the parents and patients was obtained for conducting the studies. No conflict of interest was declared by the authors. Keywords: Down syndrome, ENT pathology, ENT organs, children, adolescents.

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