scholarly journals Composites Based on Gellan Gum, Alginate and Nisin-Enriched Lipid Nanoparticles for the Treatment of Infected Wounds

2021 ◽  
Vol 23 (1) ◽  
pp. 321
Author(s):  
Katarzyna Reczyńska-Kolman ◽  
Kinga Hartman ◽  
Konrad Kwiecień ◽  
Monika Brzychczy-Włoch ◽  
Elżbieta Pamuła
Keyword(s):  
Wound Healing ◽  
Bacterial Infections ◽  
Average Particle Size ◽  
Lipid Nanoparticles ◽  
Gellan Gum ◽  
Average Particle ◽  
Hydrogel Matrix ◽  
Swelling Capacity ◽  
Infected Wounds ◽  
Methods Of Treatment

Due to growing antimicrobial resistance to antibiotics, novel methods of treatment of infected wounds are being searched for. The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide—nisin (NSN). NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL. GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%). Encapsulation of NSN within lipid nanoparticles significantly slowed down NSN release from GG-based samples for up to 24 h (as compared to GG + NSN). The most effective antimicrobial activity against Gram-positive Streptococcus pyogenes was observed for GG + NP_NSN, while in GG/Alg it was decreased by interactions between NSN and Alg, leading to NSN retention within the hydrogel matrix. All materials, except GG/Alg + NP_NSN, were cytocompatible with L929 fibroblasts and did not cause an observable delay in wound healing. We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.

scholarly journals FORMULATION AND EVALUATION OF DOXORUBICIN CONTAINING NANOGELS FOR DELIVERY TO CANCER CELLS

2018 ◽  
Vol 8 (5) ◽  
pp. 178-183
Author(s):  
Manish Kumar ◽  
Hemant K. Sharma
Keyword(s):  
Average Particle Size ◽  
Gellan Gum ◽  
Cross Linking ◽  
Average Particle ◽  
Scanning Calorimetry ◽  
Green Method ◽  
X Ray ◽  
Oppositely Charged ◽  
Chemical Cross Linking

The objective of this study is to prepare nanogels were prepared via charged gellan gum. It was prepared by in situ cross linking reaction between two oppositely charged materials by green method without use of chemical cross linking agents. The prepared nanogels were characterized by Dynamic light scattering, scanning electron microscopy, differential scanning calorimetry and X- Ray diffractometry. The prepared formulation had average particle size of 226 nm with polydispersity index of 0.3. The doxorubicin loaded nanogel demonstrated sustained release for 20 h. The prepared nanogels were hemocompatible and cyctocompatible as revealed by hemocompatibility and MTT assay respectively. All results confirmed that these nanogels can be used for cancer treatment. Keywords: Nanogel, Chitosan, Gellan gum, Doxorubicin, Cancer.

scholarly journals The Effects of Curcumin Nanoparticles Incorporated into Collagen-Alginate Scaffold on Wound Healing of Skin Tissue in Trauma Patients

Polymers ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 4291
Author(s):  
Mohammadmahdi Mobaraki ◽  
Davood Bizari ◽  
Madjid Soltani ◽  
Hadi Khshmohabat ◽  
Kaamran Raahemifar ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Average Particle Size ◽  
L929 Cell ◽  
Skin Tissue ◽  
Wound Healing Process ◽  
Average Particle ◽  
Trauma Patients ◽  
Morphological Studies

Wound healing is a biological process that is mainly crucial for the rehabilitation of injured tissue. The incorporation of curcumin (Cur) into a hydrogel system is used to treat skin wounds in different diseases due to its hydrophobic character. In this study, sodium alginate and collagen, which possess hydrophilic, low toxic, and biocompatible properties, were utilized. Collagen/alginate scaffolds were synthesized, and nanocurcumin was incorporated inside them; their interaction was evaluated by FTIR spectroscopy. Morphological studies investigated structures of the samples studied by FE-SEM. The release profile of curcumin was detected, and the cytotoxic test was determined on the L929 cell line using an MTT assay. Analysis of tissue wound healing was performed by H&E staining. Nanocurcumin was spherical, its average particle size was 45 nm, and the structure of COL/ALG scaffold was visible. The cell viability of samples was recorded in cells after 24 h incubation. Results of in vivo wound healing remarkably showed CUR-COL/ALG scaffold at about 90% (p < 0.001), which is better than that of COL/ALG, 80% (p < 0.001), and the control 73.4% (p < 0.01) groups at 14 days/ The results of the samples’ FTIR indicated that nanocurcumin was well-entrapped into the scaffold, which led to improving the wound-healing process. Our results revealed the potential of nanocurcumin incorporated in COL/ALG scaffolds for the wound healing of skin tissue in trauma patients.

scholarly journals Statistical optimization of dithranol-loaded solid lipid nanoparticles using factorial design

2011 ◽  
Vol 47 (3) ◽  
pp. 503-511 ◽  
Author(s):  
Makarand Suresh Gambhire ◽  
Mangesh Ramesh Bhalekar ◽  
Vaishali Makarand Gambhire
Keyword(s):  
Particle Size ◽  
Factorial Design ◽  
Solid Lipid Nanoparticles ◽  
Ex Vivo ◽  
Average Particle Size ◽  
Entrapment Efficiency ◽  
Lipid Nanoparticles ◽  
Average Particle ◽  
Scanning Calorimetry ◽  
Solid Lipid

This study describes a 3² full factorial experimental design to optimize the formulation of dithranol (DTH) loaded solid lipid nanoparticles (SLN) by the pre-emulsion ultrasonication method. The variables drug: lipid ratio and sonication time were studied at three levels and arranged in a 3² factorial design to study the influence on the response variables particle size and % entrapment efficiency (%EE). From the statistical analysis of data polynomial equations were generated. The particle size and %EE for the 9 batches (R1 to R9) showed a wide variation of 219-348 nm and 51.33- 71.80 %, respectively. The physical characteristics of DTH-loaded SLN were evaluated using a particle size analyzer, differential scanning calorimetry and X-ray diffraction. The results of the optimized formulation showed an average particle size of 219 nm and entrapment efficiency of 69.88 %. Ex-vivo drug penetration using rat skin showed about a 2-fold increase in localization of DTH in skin as compared to the marketed preparation of DTH.

Magnesium oxide incorporated electrospun membranes inhibit bacterial infections and promote healing process of infected wounds

2021 ◽  
Author(s):  
Mingyue Liu ◽  
Xiaoyu Wang ◽  
Haiyan Li ◽  
Changlei Xia ◽  
Zhengni Liu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Magnesium Oxide ◽  
Wound Dressing ◽  
Bacterial Infections ◽  
Healing Process ◽  
Infected Wounds ◽  
Electrospun Membranes ◽  
Nanofibrous Membranes ◽  
Mgo Nanoparticle ◽  
Healing Processes

Bacterial infections cause serious secondary damages to wounds and hinder wound healing processes. We prepared magnesium oxide (MgO) nanoparticle-incorporated nanofibrous membranes by electrospinning and investigated their potential for wound dressing...

scholarly journals Development of Polyvinyl Alcohol/Kaolin Sponges Stimulated by Marjoram as Hemostatic, Antibacterial, and Antioxidant Dressings for Wound Healing Promotion

2021 ◽  
Vol 22 (23) ◽  
pp. 13050
Author(s):  
Tamer M. Tamer ◽  
Mosa H. Alsehli ◽  
Ahmed M. Omer ◽  
Tarek H. Afifi ◽  
Maysa M. Sabet ◽  
...  
Keyword(s):  
Wound Healing ◽  
Polyvinyl Alcohol ◽  
Bacterial Infections ◽  
Antioxidant Properties ◽  
Three Dimensional ◽  
Polymeric Carrier ◽  
Antibacterial Performance ◽  
Swelling Capacity ◽  
Microbial Infections ◽  
Degradation Properties

The predominant impediments to cutaneous wound regeneration are hemorrhage and bacterial infections that lead to extensive inflammation with lethal impact. We thus developed a series of composite sponges based on polyvinyl alcohol (PVA) inspired by marjoram essential oil and kaolin (PVA/marjoram/kaolin), adopting a freeze–thaw method to treat irregular wounds by thwarting lethal bleeding and microbial infections. Microstructure analyses manifested three-dimensional interconnected porous structures for PVA/marjoram/kaolin. Additionally, upon increasing marjoram and kaolin concentrations, the pore diameters of the sponges significantly increased, recording a maximum of 34 ± 5.8 µm for PVA-M0.5-K0.1. Moreover, the porosity and degradation properties of PVA/marjoram/kaolin sponges were markedly enhanced compared with the PVA sponge with high swelling capacity. Furthermore, the PVA/marjoram/kaolin sponges exerted exceptional antibacterial performance against Escherichia coli and Bacillus cereus, along with remarkable antioxidant properties. Moreover, PVA/marjoram/kaolin sponges demonstrated significant thrombogenicity, developing high thrombus mass and hemocompatibility, in addition to their remarkable safety toward fibroblast cells. Notably, this is the first study to our knowledge investigating the effectiveness of marjoram in a polymeric carrier for prospective functioning as a wound dressing. Collectively, the findings suggest the prospective usage of the PVA-M0.5-K0.1 sponge in wound healing for hemorrhage and bacterial infection control.

scholarly journals Safety profile, antimicrobial and antibiofilm activities of a nanostructured lipid carrier containing oil and butter from Astrocaryum vulgare: in vitro studies

2021 ◽  
Vol 9 (5) ◽  
pp. 478-497
Author(s):  
Aline Rossato ◽  
Larissa da Silva Silveira ◽  
Pâmella Scharamm Oliveira ◽  
Thobias Toniolo de Souza ◽  
Ana Paula Becker ◽  
...  
Keyword(s):  
Cell Viability ◽  
Bacterial Infections ◽  
Average Particle Size ◽  
Aqueous Solubility ◽  
Human Erythrocytes ◽  
Antibiofilm Activity ◽  
Average Particle ◽  
Nanostructured Lipid Carrier ◽  
Cytotoxicity Studies

Ethnopharmacological relevance: Tucumã (Astrocaryum vulgare)is a fruit native to the Amazon region. Extracts from the peel and pulp are thought of as promising treatments for bacterial infections. The primary constituents of Tucumã oil and butter possess unsaturated carbon chains that are susceptible to oxidation by light or heat. The oils have high volatility and low aqueous solubility that limits their use without a vehicle. Nanotechnology refers to techniques to solve these problems. Nanostructured lipid carriers (NLC), for example, protect fixed oils degradation by heat or light, as well as from oxidation and evaporation, ensuring greater stability and function, thereby prolonging the useful life of the final product. Study objectives: The objective of this study was to evaluate the hemolytic, cytotoxic, antimicrobial and antibiofilm properties of an NLC containing Tucumã butter and oil soasto improve the solubility and photosensitivity of the compounds, generating better pharmacological efficacy. Materials and methods: The NLC was assessed for stability for 60 days. The cytotoxicity of nanoparticles in peripheral blood mononucleated cells was determined in culture using assays for cell viability, DNA damage, oxidative metabolism and damage to human erythrocytes. Antimicrobial activity was determined using the broth microdilution technique and antibiofilm activity according to standardized protocols. Results: The Tucumã NLC remained stable throughout the evaluated period, with pH between 5.22–5.35, monodisperse distribution (PDI<0.3) and average particle size of 170.7 ± 3nm. Cytotoxicity studies revealed that the NLC is safe and modulates inflammatory processes, demonstrated by increased cell viability and nitric oxide levels. There was low hemolytic activity of the NLC against human erythrocytes almost concentrations tested. Conclusion: Taken together, the data suggest that NLC containing Tucumã oil and butter showed antimicrobial and antibiofilm activity against organisms that cause morbidity and mortality in humans. They may be alternative solutions to public health problems related to bacterial infections.

scholarly journals Degradation of Carbazole by Microbial Cells Immobilized in Magnetic Gellan Gum Gel Beads

2007 ◽  
Vol 73 (20) ◽  
pp. 6421-6428 ◽  
Author(s):  
Xia Wang ◽  
Zhonghui Gai ◽  
Bo Yu ◽  
Jinhui Feng ◽  
Changyong Xu ◽  
...  
Keyword(s):  
Saturation Magnetization ◽  
Immobilized Cells ◽  
Average Particle Size ◽  
Gellan Gum ◽  
Average Particle ◽  
Free Cells ◽  
Polycyclic Aromatic ◽  
Degradation Activity ◽  
Hazardous Organic Compounds ◽  
20 Nm

ABSTRACT Polycyclic aromatic heterocycles, such as carbazole, are environmental contaminants suspected of posing human health risks. In this study, we investigated the degradation of carbazole by immobilized Sphingomonas sp. strain XLDN2-5 cells. Four kinds of polymers were evaluated as immobilization supports for Sphingomonas sp. strain XLDN2-5. After comparison with agar, alginate, and κ-carrageenan, gellan gum was selected as the optimal immobilization support. Furthermore, Fe3O4 nanoparticles were prepared by a coprecipitation method, and the average particle size was about 20 nm with 49.65-electromagnetic-unit (emu) g−1 saturation magnetization. When the mixture of gellan gel and the Fe3O4 nanoparticles served as an immobilization support, the magnetically immobilized cells were prepared by an ionotropic method. The biodegradation experiments were carried out by employing free cells, nonmagnetically immobilized cells, and magnetically immobilized cells in aqueous phase. The results showed that the magnetically immobilized cells presented higher carbazole biodegradation activity than nonmagnetically immobilized cells and free cells. The highest biodegradation activity was obtained when the concentration of Fe3O4 nanoparticles was 9 mg ml−1 and the saturation magnetization of magnetically immobilized cells was 11.08 emu g−1. Additionally, the recycling experiments demonstrated that the degradation activity of magnetically immobilized cells increased gradually during the eight recycles. These results support developing efficient biocatalysts using magnetically immobilized cells and provide a promising technique for improving biocatalysts used in the biodegradation of not only carbazole, but also other hazardous organic compounds.

scholarly journals Chitosan-Coated PLGA Nanoparticles Loaded with Peganum harmala Alkaloids with Promising Antibacterial and Wound Healing Activities

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2438
Author(s):  
Hassan Mohamed El-Said Azzazy ◽  
Sherif Ashraf Fahmy ◽  
Noha Khalil Mahdy ◽  
Meselhy Ragab Meselhy ◽  
Udo Bakowsky
Keyword(s):  
Wound Healing ◽  
Average Particle Size ◽  
Entrapment Efficiency ◽  
Plga Nanoparticles ◽  
Attenuated Total Reflection ◽  
Average Particle ◽  
High Antibacterial Activity ◽  
Transmission Electron ◽  
Method Optimization

Wound healing is a major healthcare concern, and complicated wounds may lead to severe outcomes such as septicemia and amputations. To date, management choices are limited, which warrants the search for new potent wound healing agents. Natural products loaded in poly (lactic-co-glycolic acid) (PLGA) coated with chitosan (CS) constitute a promising antibacterial wound healing formulation. In this work, harmala alkaloid-rich fraction (HARF) loaded into PLGA nanoparticles coated with chitosan (H/CS/PLGA NPs) were designed using the emulsion-solvent evaporation method. Optimization of the formulation variables (HARF: PLGA and CS: PLGA weight ratios, sonication time) was performed using the 33 Box–Behnken design (BBD). The optimal NPs were characterized using transmission electron microscopy (TEM) and Attenuated Total Reflection Fourier-Transformed Infrared Spectroscopy (ATR-FTIR). The prepared NPs had an average particle size of 202.27 ± 2.44 nm, a PDI of 0.23 ± 0.01, a zeta potential of 9.22 ± 0.94 mV, and an entrapment efficiency of 86.77 ± 4.18%. In vitro drug release experiments showed a biphasic pattern where an initial burst of 82.50 ± 0.20% took place in the first 2 h, which increased to 87.50 ± 0.50% over 72 h. The designed optimal H/CS/PLGA NPs exerted high antibacterial activity against Staphylococcus aureus and Escherichia coli (MIC of 0.125 and 0.06 mg/mL, respectively) compared to unloaded HARF (MIC of 0.50 mg/mL). The prepared nanoparticles were found to be biocompatible when tested on human skin fibroblasts. Moreover, the wound closure percentage after 24 h of applying H/CS/PLGA NPs was found to be 94.4 ± 8.0%, compared to free HARF and blank NPs (68.20 ± 5.10 and 50.50 ± 9.40%, respectively). In conclusion, the three components of the developed nanoformulation (PLGA, chitosan, and HARF) have synergistic antibacterial and wound healing properties for the management of infected wounds.

scholarly journals Chinese White Wax Solid Lipid Nanoparticles as a Novel Nanocarrier of Curcumin for Inhibiting the Formation of Staphylococcus aureus Biofilms

Nanomaterials ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 763 ◽  
Author(s):  
Lin Luan ◽  
Zhe Chi ◽  
Chenguang Liu
Keyword(s):  
Staphylococcus Aureus ◽  
Drug Release ◽  
Solid Lipid Nanoparticles ◽  
Average Particle Size ◽  
Entrapment Efficiency ◽  
Lipid Nanoparticles ◽  
Average Particle ◽  
Sustained Drug Release ◽  
Solid Lipid ◽  
Chinese White

Chinese white wax solid lipid nanoparticles (cwSLNs) were prepared by high shear homogenization and ultrasound methods. Using an optimized formula, spherical cwSLNs with an average particle size of 401.9 ± 21.3 nm were obtained. The cwSLNs showed high entrapment efficiency, approximately 84.6%, for loading curcumin. The curcumin loaded cwSLNs (Cur-cwSLNs) exhibited sustained drug release properties. Notably, Cur-cwSLNs had a higher drug release rate at pH 4.5 than at pH 7.4, which suggested their applicability in an acidic environment. Cur-cwSLNs were able to inhibit the growth of Staphylococcus aureus and were more effective at reducing the biofilms produced by this bacterium compared to free curcumin. This study confirmed that cwSLNs may be novel carriers for increasing the bioavailability of curcumin with the potential to inhibit the formation of S. aureus biofilms.

scholarly journals Freeze-drying of ampicillin solid lipid nanoparticles using mannitol as cryoprotectant

2015 ◽  
Vol 51 (4) ◽  
pp. 797-802 ◽  
Author(s):  
Faezeh Alihosseini ◽  
Solmaz Ghaffari ◽  
Ali Reza Dabirsiaghi ◽  
Setareh Haghighat
Keyword(s):  
Particle Size ◽  
Solid Lipid Nanoparticles ◽  
Freeze Drying ◽  
Chemical Interaction ◽  
Average Particle Size ◽  
Lipid Nanoparticles ◽  
Release Profile ◽  
Average Particle ◽  
Solid Lipid ◽  
The Stability

abstract Solid lipid nanoparticles (SLNs) are interesting colloidal drug-delivery systems, since they have all the advantages of the lipid and polymeric nanoparticles. Freeze-drying is a widely used process for improving the stability of SLNs. Cryoprotectants have been used to decrease SLN aggregations during freeze-drying. In this study Ampicillin was chosen to be loaded in a cholesterol carrier with nano size range. To support the stability of SLNs, freeze-drying was done using mannitol. Particle size, drug release profile and antibacterial effects were studied after freeze-drying in comparison with primary SLNs. Preparations with 5% mannitol showed the least particle size enlargement. The average particle size was 150 and 187 nm before and after freeze-drying, respectively. Freeze-drying did not affect the release profile of drug loaded nanopartilces. Also our study showed that lyophilization did not change the antimicrobial effect of ampicillin SLNs. DSC analysis showed probability of chemical interaction between ampicillin and cholesterol.

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