scholarly journals STOP1 Regulates LKS1 Transcription and Coordinates K+/NH4+ Balance in Arabidopsis Response to Low-K+ Stress

2021 ◽  
Vol 23 (1) ◽  
pp. 383
Author(s):  
Zhi-Fang Wang ◽  
Ting-Wei Mi ◽  
Yong-Qiang Gao ◽  
Han-Qian Feng ◽  
Wei-Hua Wu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Growth And Development ◽  
Transcript Level ◽  
Mineral Elements ◽  
The Other ◽  
Plant Growth And Development ◽  
K Uptake ◽  
Arabidopsis Root ◽  
Leaf Chlorosis ◽  
Low K

Potassium and nitrogen are essential mineral elements for plant growth and development. The protein kinase LKS1/CIPK23 is involved in both K+ and NH4+ uptake in Arabidopsis root. The transcripts of LKS1 can be induced by low K+ (0.1 mM) and high NH4+ (30 mM); however, the molecular mechanism is still unknown. In this study, we isolated the transcription factor STOP1 that positively regulates LKS1 transcription in Arabidopsis responses to both low-K+ and high-NH4+ stresses. STOP1 proteins can directly bind to the LKS1 promoter, promoting its transcription. The stop1 mutants displayed a leaf chlorosis phenotype similar to lks1 mutant when grown on low-K+ and high-NH4+ medium. On the other hand, STOP1 overexpressing plants exhibited a similar tolerant phenotype to LKS1 overexpressing plants. The transcript level of STOP1 was only upregulated by low K+ rather than high NH4+; however, the accumulation of STOP1 protein in the nucleus was required for the upregulation of LKS1 transcripts in both low-K+ and high-NH4+ responses. Our data demonstrate that STOP1 positively regulates LKS1 transcription under low-K+ and high-NH4+ conditions; therefore, LKS1 promotes K+ uptake and inhibits NH4+ uptake. The STOP1/LKS1 pathway plays crucial roles in K+ and NH4+ homeostasis, which coordinates potassium and nitrogen balance in plants in response to external fluctuating nutrient levels.

scholarly journals ZMK1 Is Involved in K+ Uptake and Regulated by Protein Kinase ZmCIPK23 in Zea mays

2021 ◽  
Vol 12 ◽  
Author(s):  
Wu Han ◽  
Yun Ji ◽  
Wei Wu ◽  
Jin-Kui Cheng ◽  
Han-Qian Feng ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Growth And Development ◽  
Mineral Elements ◽  
Plant Roots ◽  
Plant Growth And Development ◽  
K Uptake ◽  
Uptake Mechanism ◽  
Uptake Activity ◽  
Electrophysiological Characterization ◽  
Low K

Potassium (K+) is one of essential mineral elements for plant growth and development. K+ channels, especially AKT1-like channels, play crucial roles in K+ uptake in plant roots. Maize is one of important crops; however, the K+ uptake mechanism in maize is little known. Here, we report the physiological functions of K+ channel ZMK1 in K+ uptake and homeostasis in maize. ZMK1 is a homolog of Arabidopsis AKT1 channel in maize, and mainly expressed in maize root. Yeast complementation experiments and electrophysiological characterization in Xenopus oocytes indicated that ZMK1 could mediate K+ uptake. ZMK1 rescued the low-K+-sensitive phenotype of akt1 mutant and enhanced K+ uptake in Arabidopsis. Overexpression of ZMK1 also significantly increased K+ uptake activity in maize, but led to an oversensitive phenotype. Similar to AKT1 regulation, the protein kinase ZmCIPK23 interacted with ZMK1 and phosphorylated the cytosolic region of ZMK1, activating ZMK1-mediated K+ uptake. ZmCIPK23 could also complement the low-K+-sensitive phenotype of Arabidopsis cipk23/lks1 mutant. These findings demonstrate that ZMK1 together with ZmCIPK23 plays important roles in K+ uptake and homeostasis in maize.

scholarly journals A Screen for Genes That Function in Abscisic Acid Signaling in Arabidopsis thaliana

Genetics ◽  
2002 ◽  
Vol 161 (3) ◽  
pp. 1247-1255 ◽  
Author(s):  
Eiji Nambara ◽  
Masaharu Suzuki ◽  
Suzanne Abrams ◽  
Donald R McCarty ◽  
Yuji Kamiya ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Growth And Development ◽  
Plant Hormone ◽  
The Other ◽  
Aba Signaling ◽  
Wild Type ◽  
Plant Growth And Development ◽  
Diverse Range ◽  
Abscisic Acid Signaling ◽  
Aba Insensitive

Abstract The plant hormone abscisic acid (ABA) controls many aspects of plant growth and development under a diverse range of environmental conditions. To identify genes functioning in ABA signaling, we have carried out a screen for mutants that takes advantage of the ability of wild-type Arabidopsis seeds to respond to (−)-(R)-ABA, an enantiomer of the natural (+)-(S)-ABA. The premise of the screen was to identify mutations that preferentially alter their germination response in the presence of one stereoisomer vs. the other. Twenty-six mutants were identified and genetic analysis on 23 lines defines two new loci, designated CHOTTO1 and CHOTTO2, and a collection of new mutant alleles of the ABA-insensitive genes, ABI3, ABI4, and ABI5. The abi5 alleles are less sensitive to (+)-ABA than to (−)-ABA. In contrast, the abi3 alleles exhibit a variety of differences in response to the ABA isomers. Genetic and molecular analysis of these alleles suggests that the ABI3 transcription factor may perceive multiple ABA signals.

Cloning and expression analysis of ZmABI3 gene in Zea mays

2017 ◽  
Vol 42 (3) ◽  
Author(s):  
Qiaoyun Weng ◽  
Jinhui Song ◽  
Hailian Ma ◽  
Jincheng Yuan ◽  
Yanmin Zhao ◽  
...  
Keyword(s):  
Zea Mays ◽  
Transcription Factor ◽  
Plant Growth ◽  
Plant Species ◽  
Expression Analysis ◽  
Growth And Development ◽  
Cloning And Expression ◽  
Plant Growth And Development ◽  
New Gene ◽  
B3 Domain

AbstractObjectiveABI3 is a B3 domain transcription factor existed in various plant species. Studies showed that ABI3 play important role in plant growth and development.MethodsTheResultsOne gene contained B3 domain was isolated from maize, designated asConclusionA new gene contained a B3 domain was cloned and named as

Possible Basic and Specific Functions of Plant Uncoupling Proteins (pUCP)

2001 ◽  
Vol 21 (2) ◽  
pp. 237-245 ◽  
Author(s):  
Petr Ježek ◽  
Jirí Borecký ◽  
Markêta Zácková ◽  
Alexandre D. T. Costa ◽  
Paulo Arruda
Keyword(s):  
Low Temperatures ◽  
Growth And Development ◽  
Uncoupling Protein ◽  
Atp Synthesis ◽  
Uncoupling Proteins ◽  
The Other ◽  
Oxygen Species ◽  
Metabolic Rates ◽  
Plant Growth And Development ◽  
Functional Localization

Evidence has been provided that the plant uncoupling proteins (pUCP) play basic physiological roles similar to the other uncoupling protein subfamily members (mammalian UCP1,2,3,4 and BMCP) and are effective in the situations of slight uncoupling that leads to: (1) accelerated respiration and metabolic rates that are beneficial to plant growth and development; (2) decreased formation of reactive oxygen species in mitochondria; and, (3) mild thermogenesis, inevitably accompanying the previous two phenomena. Hypothetically, specific physiological roles of pUCP such as cut off of ATP synthesis could be manifested in connection with climacteric respiratory rise during fruit ripening, seed dormancy, and plant senescence. pUCP might also facilitate growth under low temperatures, e.g., during seed germination or in roots. The existence of these specific roles is suggested by the immunochemical and functional localization of pUCP in mitochondria of fruits, seeds and roots of various plant species.

scholarly journals The Evaluation of Factors Determining the Health Condition of Acer Platanoides and Tilia Cordata Plantings in Selected Sites of Urban Greenery in Poznań, Poland

2013 ◽  
Vol 53 (1) ◽  
pp. 60-64 ◽  
Author(s):  
Barbara Wilkaniec ◽  
Beata Borowiak-Sobkowiak ◽  
Agnieszka Wilkaniec ◽  
Włodzimierz Breś ◽  
Dorota Frużyńska-Jóźwiak
Keyword(s):  
Growth And Development ◽  
Dominant Species ◽  
Visual Inspection ◽  
Health Condition ◽  
The Other ◽  
Tilia Cordata ◽  
Acer Platanoides ◽  
Plant Growth And Development ◽  
Urban Greenery ◽  
Norway Maple

Abstract Heavily urbanised areas are not a favourable habitat for plant growth and development. On the other hand, urbanised areas can be a favourable habitat for harmful fauna. Tin our study, those trees growing along the roadside were more heavily infested by pests than trees growing in parks. Three orders of insects, mites and lugs were identified on the Norway maple. The dominant insect species was Periphyllus aceris. On small-leaved lime four insect orders (the dominant species was and Eucallipterus tiliae) and mites were found. An overall visual inspection confirmed that of maple and lime growing in city parks were in better condition than those growing by roads.

scholarly journals Rice Potassium Transporter OsHAK8 Mediates K+ Uptake and Translocation in Response to Low K+ Stress

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaohui Wang ◽  
Junfeng Li ◽  
Fei Li ◽  
Yu Pan ◽  
Dan Cai ◽  
...  
Keyword(s):  
Crop Production ◽  
Cell Types ◽  
Plant Growth And Development ◽  
Ion Content ◽  
Chemical Fertilizers ◽  
K Uptake ◽  
Stunted Growth ◽  
Use Efficiency ◽  
Different Cell Types ◽  
Low K

Potassium (K+) levels in the soil often limit plant growth and development. As a result, crop production largely relies on the heavy use of chemical fertilizers, presenting a challenging problem in sustainable agriculture. To breed crops with higher K+-use efficiency (KUE), we must learn how K+ is acquired from the soil by the root system and transported to the rest of the plant through K+ transporters. In this study, we identified the function of the rice K+ transporter OsHAK8, whose expression level is downregulated in response to low-K+ stress. When OsHAK8 was disrupted by CRISPR/Cas9-mediated mutagenesis, Oshak8 mutant plants showed stunted growth, especially under low-K+ conditions. Ion content analyses indicated that K+ uptake and root-to-shoot K+ transport were significantly impaired in Oshak8 mutants under low-K+ conditions. As the OsHAK8 gene was broadly expressed in different cell types in the roots and its protein was targeted to the plasma membrane, we propose that OsHAK8 serves as a major transporter for both uptake and root-to-shoot translocation in rice plants.

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