Asymmetric Interaction between Aphis spiraecola and Toxoptera citricida on Sweet Orange Induced by Pre-Infestation

Indirect interactions between herbivorous insects that share the same host have been focused on insects feeding on herbaceous plants, while few studies investigate similar interactions on woody plants. We investigated performance and feeding behavior of two citrus aphids, Aphis spiraecola Patch and Toxoptera citricida Kirkaldy, on sweet orange as affected by prior infestation of conspecifics and heterospecifics. Results showed that pre-infestation-induced interactions between A. spiraecola and T. citricida were asymmetric, with A. spiraecola gaining more fitness. In detail, pre-infestation by A. spiraecola decreased adult weight, enhanced survival rate and accelerated phloem sap acceptance of conspecifics. However, A. spiraecola pre-infestation did not affect performance or feeding behavior of T. citricida. In another infestation sequence, the pre-infestation of T. citricida did not affect conspecifics, but positively affected heterospecifics, indicated as a decreased pre-reproductive period, enhanced survival rate, adult weight, fecundity, and feeding efficiency, i.e., faster access and acceptance of phloem sap, and longer phloem sap ingestion duration. Furthermore, we found A. spiraecola pre-infestation enhanced amino acid concentration, amino acid to sugar ratio, activated salicylic acid and jasmonic acid marker gene expression, while T. citricida pre-infestation only depressed jasmonic acid marker gene expression. Changes in nutrient and phytohormone-dependent defense probably underlie the asymmetric effect.

Download Full-text

Faculty Opinions recommendation of Long-term stability of marker gene expression in Prunus subhirtella: a model fruit tree species.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1058806.510735 ◽

2007 ◽

Author(s):

Niklaus Ammann

Keyword(s):

Gene Expression ◽

Tree Species ◽

Marker Gene ◽

Fruit Tree ◽

Term Stability ◽

Long Term Stability ◽

Marker Gene Expression

Download Full-text

Eosinophil Granular Protein(s) Modulate Tumor Metastasis Marker Gene Expression

10.21236/ada473779 ◽

2007 ◽

Author(s):

Paulette Furbert-Harris

Keyword(s):

Gene Expression ◽

Tumor Metastasis ◽

Marker Gene ◽

Protein S ◽

Marker Gene Expression

Download Full-text

Comparative Analysis of Cartilage Marker Gene Expression Patterns during Axolotl and Xenopus Limb Regeneration

PLoS ONE ◽

10.1371/journal.pone.0133375 ◽

2015 ◽

Vol 10 (7) ◽

pp. e0133375 ◽

Cited By ~ 8

Author(s):

Kazumasa Mitogawa ◽

Aki Makanae ◽

Ayano Satoh ◽

Akira Satoh

Keyword(s):

Gene Expression ◽

Comparative Analysis ◽

Expression Patterns ◽

Marker Gene ◽

Limb Regeneration ◽

Gene Expression Patterns ◽

Marker Gene Expression

Download Full-text

High glucose-associated osmolality promotes adipocytogenic differentiation of primary rat osteoblasts in a protein kinase A and phosphatidylinositol 3-kinase/Akt-dependent manner

Biologia ◽

10.1515/biolog-2015-0156 ◽

2015 ◽

Vol 70 (10) ◽

Author(s):

Yu Zhang ◽

Pu Feng ◽

Jianhong Yang

Keyword(s):

Gene Expression ◽

Protein Kinase ◽

Protein Kinase A ◽

Real Time ◽

High Glucose ◽

Marker Gene ◽

Dependent Manner ◽

Phosphatidylinositol 3 Kinase ◽

Marker Gene Expression ◽

Kinase A

AbstractIncreased risk of osteoporosis in patients with diabetes mellitus may be related to hyperglycemia. However, the potential mechanisms accounting for diabetic bone disorder remain unresolved. The present study investigated the effects of high glucose-associated osmolality on differentiation of primary rat calvarial osteoblasts. Osteoblastogenic differentiation was determined by bone nodule staining for mineralization assay, enzyme-linked immunosorbent assay for type I collagen production and real-time polymerase chain reaction (PCR) for osteoblastogenic marker gene expression. Adipocytogenic differentiation was assessed by oil red O staining for lipid accumulation and real-time PCR for adipocytogenic marker gene expression. The phosphorylations of protein kinase A (PKA) and Akt were measured with or without specific inhibitors to confirm osmolality involved signalling pathways. The results showed that high glucose-associated osmolality significantly promoted adipocytogenic differentiation, manifested by increased lipid droplet formation and gene expression of adipocytogenic markers including adipocyte fatty acid binding protein (aP2), adipsin and peroxisome proliferator-activated receptor gamma (PPARγ). Meanwhile, high glucose-associated osmolality inhibited osteoblastogenic differentiation, characterized by decreased collagen I protein production and cell mineralization, as well as gene expression of osteoblastogenic markers including collagen I, osteocalcin and runt-related transcription factor 2 (Runx2). More importantly, we demonstrated for the first time that high glucose-associated osmolality induced adipocytogenic differentiation and suppressed osteoblastogenic differentiation in a PKA and phosphatidylinositol 3-kinase (PI3K)/Akt-dependent manner. These results indicated that osmolality was involved in high glucose-induced osteoblast trans-differentiation into adipocyte-like cell and suppression of cellular osmolality could provide novel therapeutic approach for diabetic osteopenia.

Download Full-text

Widespread and efficient marker gene expression in the airway epithelia of fetal sheep after minimally invasive tracheal application of recombinant adenovirus in utero

Gene Therapy ◽

10.1038/sj.gt.3302130 ◽

2003 ◽

Vol 11 (1) ◽

pp. 70-78 ◽

Cited By ~ 48

Author(s):

D Peebles ◽

LG Gregory ◽

A David ◽

M Themis ◽

SN Waddington ◽

...

Keyword(s):

Gene Expression ◽

Minimally Invasive ◽

Recombinant Adenovirus ◽

Marker Gene ◽

In Utero ◽

Fetal Sheep ◽

Airway Epithelia ◽

Marker Gene Expression

Download Full-text

Ret isoform function and marker gene expression in the enteric nervous system is conserved across diverse vertebrate species

Mechanisms of Development ◽

10.1016/j.mod.2008.04.006 ◽

2008 ◽

Vol 125 (8) ◽

pp. 687-699 ◽

Cited By ~ 38

Author(s):

Tiffany A. Heanue ◽

Vassilis Pachnis

Keyword(s):

Gene Expression ◽

Nervous System ◽

Enteric Nervous System ◽

Marker Gene ◽

Vertebrate Species ◽

Marker Gene Expression

Download Full-text

miR-539 mediates osteoblast mineralization by regulating Distal-less genes 2 in MC3T3-E1 cell line

Open Life Sciences ◽

10.1515/biol-2017-0034 ◽

2017 ◽

Vol 12 (1) ◽

pp. 294-299 ◽

Cited By ~ 1

Author(s):

Jianguo Han ◽

Li Su ◽

Chunyang Zhang ◽

Rongcai Jiang

Keyword(s):

Gene Expression ◽

Alkaline Phosphatase ◽

Cell Line ◽

Osteoblast Differentiation ◽

Marker Gene ◽

Inhibitory Effect ◽

Negative Regulator ◽

Expression Level ◽

Matrix Mineralization ◽

Marker Gene Expression

AbstractmicroRNAs (miRNAs) play an important role in osteoblast differentiation. However, the mechanisms of miRNAs regulating osteoblast mineralization still needs to be further cleared. Distal-less genes 2 (Dlx2) plays an important role in osteoblast differentiation. We have found that miR-539 was significantly downregulated and Dlx2 was found to be inversely correlated with miR-539 in MC3T3-E1 cell line during osteoblast mineralization. The overexpression of miR-539 significantly decreased the expression level of Dlx2 and suppressed the osteogenic marker gene expression level, alkaline phosphatase activity and matrix mineralization. Our study showed that miR-539 was a negative regulator in osteoblast mineralization and that the targeting of Dlx2 gene partly contributes to this inhibitory effect exerted by miR-539.

Download Full-text