Bedside 3D Visualization of Lymphatic Vessels with a Handheld Multispectral Optoacoustic Tomography Device

Identification of lymphatics by Indocyanine Green (ICG) lymphography in patients with severe lymphedema is limited due to the overlying dermal backflow. Nor can the method detect deep and/or small vessels. Multispectral optoacoustic tomography (MSOT), a real-time three- dimensional (3D) imaging modality which allows exact spatial identification of absorbers in tissue such as blood and injected dyes can overcome these hurdles. However, MSOT with a handheld probe has not been performed yet in lymphedema patients. We conducted a pilot study in 11 patients with primary and secondary lymphedema to test whether lymphatic vessels could be detected with a handheld MSOT device. In eight patients, we could not only identify lymphatics and veins but also visualize their position and contractility. Furthermore, deep lymphatic vessels not traceable by ICG lymphography and lymphatics covered by severe dermal backflow, could be clearly identified by MSOT. In three patients, two of which had advanced stage lymphedema, only veins but no lymphatic vessels could be identified. We found that MSOT can identify and image lymphatics and veins in real-time and beyond the limits of near-infrared technology during a single bedside examination. Given its easy use and high accuracy, the handheld MSOT device is a promising tool in lymphatic surgery.

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Volumetric Real-Time Tracking of Peripheral Human Vasculature With GPU-Accelerated Three-Dimensional Optoacoustic Tomography

IEEE Transactions on Medical Imaging ◽

10.1109/tmi.2013.2272079 ◽

2013 ◽

Vol 32 (11) ◽

pp. 2050-2055 ◽

Cited By ~ 78

Author(s):

X. Luis Dean-Ben ◽

Ali Ozbek ◽

Daniel Razansky

Keyword(s):

Real Time ◽

Three Dimensional ◽

Optoacoustic Tomography ◽

Real Time Tracking

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Real-Time 3D Visualization and Navigation Using Fiber-Based Endoscopic System for Arthroscopic Surgery

Journal of Advanced Computational Intelligence and Intelligent Informatics ◽

10.20965/jaciii.2016.p0735 ◽

2016 ◽

Vol 20 (5) ◽

pp. 735-742 ◽

Cited By ~ 1

Author(s):

Zhongjie Long ◽

◽

Kouki Nagamune ◽

Ryosuke Kuroda ◽

Masahiro Kurosaka ◽

...

Keyword(s):

Knee Joint ◽

Real Time ◽

3D Visualization ◽

Arthroscopic Surgery ◽

Three Dimensional ◽

Joint Surface ◽

Computer Assisted ◽

Normal Vector ◽

3D Navigation ◽

Freehand Technique

Three-dimensional (3D) navigation using a computer-assisted technique is being increasingly performed in minimally invasive surgical procedures because it can provide stereoscopic information regarding the operating field to the surgeon. In this paper, the development of a real-time arthroscopic system utilizing an endoscopic camera and optical fiber to navigate a normal vector for a reconstructed knee joint surface is described. A specific navigation approach suitable for use in a rendered surface was presented in extenso. A small-sized endoscopic tube was utilized arthroscopically on a cadaveric knee joint to show the potential application of the developed system. Experimental results of underwater navigation on a synthetic knee joint showed that our system allows for a higher accuracy than a freehand technique. The mean angle of navigation for the proposed technique is 9.5circ (range, 5circ to 17circ; SD, 2.86circ) versus 14.8circ (range, 6circ to 26circ; SD, 7.53circ) and 12.6circ (range, 4circ to 17circ; SD, 3.98circ) for two sites using a freehand technique.

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Simultaneous visualization of tumour oxygenation, neovascularization and contrast agent perfusion by real-time three-dimensional optoacoustic tomography

European Radiology ◽

10.1007/s00330-015-3980-0 ◽

2015 ◽

Vol 26 (6) ◽

pp. 1843-1851 ◽

Cited By ~ 37

Author(s):

Vladimir Ermolayev ◽

Xose Luis Dean-Ben ◽

Subhamoy Mandal ◽

Vasilis Ntziachristos ◽

Daniel Razansky

Keyword(s):

Contrast Agent ◽

Real Time ◽

Three Dimensional ◽

Optoacoustic Tomography ◽

Tumour Oxygenation ◽

Simultaneous Visualization

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Real three‐dimensional approach vs two‐dimensional camera with and without real‐time near‐infrared imaging with indocyanine green for detection of endometriosis: A case‐control study

Acta Obstetricia Et Gynecologica Scandinavica ◽

10.1111/aogs.13866 ◽

2020 ◽

Vol 99 (10) ◽

pp. 1330-1338

Author(s):

Giuseppe Vizzielli ◽

Francesco Cosentino ◽

Diego Raimondo ◽

Luigi C. Turco ◽

Virginia Vargiu ◽

...

Keyword(s):

Indocyanine Green ◽

Real Time ◽

Near Infrared ◽

Case Control Study ◽

Infrared Imaging ◽

Three Dimensional ◽

Case Control ◽

Two Dimensional ◽

Dimensional Approach ◽

Control Study

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Characterization of Near-Infrared Functional Lymphatic Imaging in the Rat Tail Model

Volume 1B: Extremity; Fluid Mechanics; Gait; Growth, Remodeling, and Repair; Heart Valves; Injury Biomechanics; Mechanotransduction and Sub-Cellular Biophysics; MultiScale Biotransport; Muscle, Tendon and Ligament; Musculoskeletal Devices; Multiscale Mechanics; Thermal Medicine; Ocular Biomechanics; Pediatric Hemodynamics; Pericellular Phenomena; Tissue Mechanics; Biotransport Design and Devices; Spine; Stent Device Hemodynamics; Vascular Solid Mechanics; Student Paper and Design Competitions ◽

10.1115/sbc2013-14765 ◽

2013 ◽

Author(s):

Michael Weiler ◽

J. Brandon Dixon

Keyword(s):

Near Infrared ◽

Immune Cell ◽

Imaging Modality ◽

Lymphatic Vessels ◽

The Body ◽

Cell Trafficking ◽

Nir Imaging ◽

Lymphatic Vasculature ◽

Rat Tail Model ◽

Lymphatic Imaging

The lymphatic vasculature is present in nearly every tissue of the body to serve essential functions in fluid homeostasis, immune cell trafficking, and lipid transport, and it has been implicated in the progression of several diseases. Despite the critical roles that this system performs, very little is known about the lymphatic vasculature in comparison to the blood vasculature, which can be attributed, in part, to the difficulty associated with imaging lymphatic vessels. With the growing interest in studying lymphatics, near-infrared (NIR) imaging has emerged in the literature as a novel lymphatic imaging modality to simultaneously improve spatial resolution to visualize small initial lymphatics and increase temporal resolution to capture the dynamic lymphatic pump function responsible for fluid propulsion.

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Noninvasive Real-Time Visualization of Multiple Cerebral Hemodynamic Parameters in Whole Mouse Brains Using Five-Dimensional Optoacoustic Tomography

Journal of Cerebral Blood Flow & Metabolism ◽

10.1038/jcbfm.2014.249 ◽

2015 ◽

Vol 35 (4) ◽

pp. 531-535 ◽

Cited By ~ 53

Author(s):

Sven Gottschalk ◽

Thomas Felix Fehm ◽

Xosé Luís Deán-Ben ◽

Daniel Razansky

Keyword(s):

Real Time ◽

Cerebral Blood Volume ◽

Functional Neuroimaging ◽

Three Dimensional ◽

Blood Oxygenation ◽

Hemodynamic Parameters ◽

Label Free ◽

Optoacoustic Tomography ◽

Real Time Visualization ◽

Tomographic Data

Current functional neuroimaging methods are not adequate for high-resolution whole-brain visualization of neural activity in real time. Here, we show imaging of fast hemodynamic changes in deep mouse brain using fully noninvasive acquisition of five-dimensional optoacoustic data from animals subjected to oxygenation stress. Multispectral video-rate acquisition of three-dimensional tomographic data enables simultaneous label-free assessment of multiple brain hemodynamic parameters, including blood oxygenation, total hemoglobin, cerebral blood volume, oxygenized and deoxygenized hemoglobin, in real time. The unprecedented results indicate that the proposed methodology may serve as a powerful complementary, and potentially superior, method for functional neuroimaging studies in rodents.

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Research on Real-Time 3D Visualization of Dam Transport on Network Environment

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.256-259.2431 ◽

2012 ◽

Vol 256-259 ◽

pp. 2431-2434

Author(s):

Xu Liu ◽

Bo Cui ◽

Da Wei Tong

Keyword(s):

Real Time ◽

Visual Information ◽

3D Visualization ◽

Three Dimensional ◽

Earth Dam ◽

Practical Significance ◽

Dump Truck ◽

Network Environment ◽

Complex Technology ◽

Construction Organization

According to the high earth dam with a large volume, high construction intensity, tense schedule, numerous construction machines, complex technology, and a lot of unexpected risk factors, the construction transportation becomes poor if the arrangement for transportation to the dam is unreasonable, which may result in schedule delays. This paper discusses the system of the real-time 3D visualization on network environment which is based on Unity3D engine, and the query of real-time three-dimensional visual information for transportation to the dam on the network environment is achieved. Users can have a real-time view of the dump truck during transportation and optimize the arrangements of construction organization. The results of research have great practical significance.

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Methodology for Addressing Infectious Aerosol Persistence in Real-Time Using Sensor Network

Sensors ◽

10.3390/s21113928 ◽

2021 ◽

Vol 21 (11) ◽

pp. 3928

Author(s):

Sepehr Makhsous ◽

Joelle M. Segovia ◽

Jiayang He ◽

Daniel Chan ◽

Larry Lee ◽

...

Keyword(s):

Real Time ◽

Sensor Network ◽

High Efficiency ◽

3D Visualization ◽

Three Dimensional ◽

Local Area ◽

Intervention Strategies ◽

Dental Restoration ◽

Transmission Risk ◽

School Of Dentistry

Human exposure to infectious aerosols results in the transmission of diseases such as influenza, tuberculosis, and COVID-19. Most dental procedures generate a significant number of aerosolized particles, increasing transmission risk in dental settings. Since the generation of aerosols in dentistry is unavoidable, many clinics have started using intervention strategies such as area-filtration units and extraoral evacuation equipment, especially under the relatively recent constraints of the pandemic. However, the effectiveness of these devices in dental operatories has not been studied. Therefore, the ability of dental personnel to efficiently position and operate such instruments is also limited. To address these challenges, we utilized a real-time sensor network for assessment of aerosol dynamics during dental restoration and cleaning producers with and without intervention. The strategies tested during the procedures were (i) local area High-Efficiency Particle Air (HEPA) filters and (ii) Extra-Oral Suction Device (EOSD). The study was conducted at the University of Washington School of Dentistry using a network of 13 fixed sensors positioned within the operatory and one wearable sensor worn by the dental operator. The sensor network provides time and space-resolved particulate matter (PM) data. Three-dimensional (3D) visualization informed aerosol persistence in the operatory. It was found that area filters did not improve the overall aerosol concentration in dental offices in a significant way. A decrease in PM concentration by an average of 16% was observed when EOSD equipment was used during the procedures. The combination of real-time sensors and 3D visualization can provide dental personnel and facility managers with actionable feedback to effectively assess aerosol transmission in medical settings and develop evidence-based intervention strategies.

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Development of three-dimensional physiological function imaging of biological body by transillumination imaging using near infrared light - preliminary research -

10.29007/dvzs ◽

2020 ◽

Author(s):

Ngoc An Dang Nguyen ◽

Phuong Anh Bui ◽

Anh Tu Tran ◽

Trung An Dang Nguyen ◽

Van Chinh Nguyen ◽

...

Keyword(s):

Near Infrared ◽

Physiological Function ◽

Imaging Modality ◽

Three Dimensional ◽

The Body ◽

Infrared Light ◽

Body Parts ◽

Two Dimensional ◽

Animal Body ◽

Preliminary Research

In biological tissue, there are different kinds of endogenous chromophores. Their absorption spectra in the optical range are sensitive to the physiological change of the animal body. In the near-infrared region (700-1200 nm wavelength), hemoglobin has a characteristic absorption spectrum which is dependent on its redox state. Therefore, the functional information inside the animal body could be obtained noninvasively by measuring the transmitted light. By detecting the change of the absorption characteristics of the animal body, the functional change inside the body can be detected in a two-dimensional transillumination image. In this paper, we propose preliminary research on developing a novel imaging modality of biological body parts. Using the two-dimensional images obtained in many different orientations, three-dimensional physiological function imaging of the biological body by transillumination could be expected. This paper presents a preliminary result in the experiment to show the feasibility of this technique.

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