scholarly journals An Overview of the Use of Equine Collagen as Emerging Material for Biomedical Applications

2020 ◽  
Vol 11 (4) ◽  
pp. 79
Author(s):  
Nunzia Gallo ◽  
Maria Lucia Natali ◽  
Alessandro Sannino ◽  
Luca Salvatore
Keyword(s):  
Immune Response ◽  
Clinical Trials ◽  
Medical Devices ◽  
Biomedical Applications ◽  
Life Science ◽  
Type I Collagen ◽  
Experimental Studies ◽  
Attractive Alternative ◽  
Type I ◽  
Implantable Medical Devices

Type I collagen has always aroused great interest in the field of life-science and bioengineering, thanks to its favorable structural properties and bioactivity. For this reason, in the last five decades it has been widely studied and employed as biomaterial for the manufacture of implantable medical devices. Commonly used sources of collagen are represented by bovine and swine but their applications are limited because of the zoonosis transmission risks, the immune response and the religious constrains. Thus, type-I collagen isolated from horse tendon has recently gained increasing interest as an attractive alternative, so that, although bovine and porcine derived collagens still remain the most common ones, more and more companies started to bring to market a various range of equine collagen-based products. In this context, this work aims to overview the properties of equine collagen making it particularly appealing in medicine, cosmetics and pharmaceuticals, as well as its main biomedical applications and the currently approved equine collagen-based medical devices, focusing on experimental studies and clinical trials of the last 15 years. To the best of our knowledge, this is the first review focusing on the use of equine collagen, as well as on equine collagen-based marketed products for healthcare.

scholarly journals A Trifecta of New Insights into Ovine Footrot for Infection Drivers, Immune Response and Host Pathogen Interactions.

2021 ◽  
Author(s):  
Adam M. Blanchard ◽  
Ceri E. Staley ◽  
Laurence Shaw ◽  
Sean R Wattegedera ◽  
Christina-Marie Baumbach ◽  
...  
Keyword(s):  
Immune Response ◽  
Type I Collagen ◽  
Global Analysis ◽  
Bacterial Species ◽  
Skin Barrier ◽  
Control Measures ◽  
Skin Barrier Function ◽  
Type I ◽  
Bacterial Populations

Footrot is a polymicrobial infectious disease in sheep causing severe lameness, leading to one of the industry’s biggest welfare problems. The complex aetiology of footrot makes in-situ or in-vitro investigations difficult. Computational methods offer a solution to understanding the bacteria involved, how they may interact with the host and ultimately providing a way to identify targets for future hypotheses driven investigative work. Here we present the first combined global analysis of the bacterial community transcripts together with the host immune response in healthy and diseased ovine feet during a natural polymicrobial infection state using metatranscriptomics. The intra tissue and surface bacterial populations and the most abundant bacterial transcriptome were analysed, demonstrating footrot affected skin has a reduced diversity and increased abundances of, not only the causative bacteria Dichelobacter nodosus , but other species such as Mycoplasma fermentans and Porphyromonas asaccharolytica . Host transcriptomics reveals a suppression of biological processes relating to skin barrier function, vascular functions, and immunosurveillance in unhealthy interdigital skin, supported by histological findings that type I collagen (associated with scar tissue formation) is significantly increased in footrot affected interdigital skin comparted to outwardly healthy skin. Finally, we provide some interesting indications of host and pathogen interactions associated with virulence genes and the host spliceosome which could lead to the identification of future therapeutic targets. Impact Statement Lameness in sheep is a global welfare and economic concern and footrot is the leading cause of lameness, affecting up to 70% of flocks in the U.K. Current methods for control of this disease are labour intensive and account for approximately 65% of antibiotic use in sheep farming, whilst preventative vaccines suffer from poor efficacy due to antigen competition. Our limited understanding of cofounders, such as strain variation and polymicrobial nature of infection mean new efficacious, affordable and scalable control measures are not receiving much attention. Here we examine the surface and intracellular bacterial populations and propose potential interactions with the host. Identification of these key bacterial species involved in the initiation and progression of disease and the host immune mechanisms could help form the basis of new therapies.

Wearable and Implantable Sensors for Biomedical Applications

2018 ◽  
Vol 11 (1) ◽  
pp. 127-146 ◽  
Author(s):  
Hatice Ceylan Koydemir ◽  
Aydogan Ozcan
Keyword(s):  
Mobile Health ◽  
Medical Devices ◽  
Biomedical Applications ◽  
Health Management ◽  
Great Promise ◽  
Implantable Sensors ◽  
Implantable Medical Devices ◽  
Health Technologies ◽  
Materials Used ◽  
Improving Patient Care

Mobile health technologies offer great promise for reducing healthcare costs and improving patient care. Wearable and implantable technologies are contributing to a transformation in the mobile health era in terms of improving healthcare and health outcomes and providing real-time guidance on improved health management and tracking. In this article, we review the biomedical applications of wearable and implantable medical devices and sensors, ranging from monitoring to prevention of diseases, as well as the materials used in the fabrication of these devices and the standards for wireless medical devices and mobile applications. We conclude by discussing some of the technical challenges in wearable and implantable technology and possible solutions for overcoming these difficulties.

scholarly journals Effect of hydroxylysine-O-glycosylation on the structure of type I collagen molecule: A computational study

Glycobiology ◽  
2020 ◽  
Vol 30 (10) ◽  
pp. 830-843
Author(s):  
Ming Tang ◽  
Xiaocong Wang ◽  
Neha S Gandhi ◽  
Bethany Lachele Foley ◽  
Kevin Burrage ◽  
...  
Keyword(s):  
Force Field ◽  
Type I Collagen ◽  
Computational Study ◽  
Experimental Studies ◽  
Helical Structure ◽  
Atomic Level ◽  
Collagen Molecule ◽  
Type I ◽  
Dynamic Simulations ◽  
Force Field Parameters

Abstract Collagen undergoes many types of post-translational modifications (PTMs), including intracellular modifications and extracellular modifications. Among these PTMs, glycosylation of hydroxylysine (Hyl) is the most complicated. Experimental studies demonstrated that this PTM ceases once the collagen triple helix is formed and that Hyl-O-glycosylation modulates collagen fibrillogenesis. However, the underlying atomic-level mechanisms of these phenomena remain unclear. In this study, we first adapted the force field parameters for O-linkages between Hyl and carbohydrates and then investigated the influence of Hyl-O-glycosylation on the structure of type I collagen molecule, by performing comprehensive molecular dynamic simulations in explicit solvent of collagen molecule segment with and without the glycosylation of Hyl. Data analysis demonstrated that (i) collagen triple helices remain in a triple-helical structure upon glycosylation of Hyl; (ii) glycosylation of Hyl modulates the peptide backbone conformation and their solvation environment in the vicinity and (iii) the attached sugars are arranged such that their hydrophilic faces are well exposed to the solvent, while their hydrophobic faces point towards the hydrophobic portions of collagen. The adapted force field parameters for O-linkages between Hyl and carbohydrates will aid future computational studies on proteins with Hyl-O-glycosylation. In addition, this work, for the first time, presents the detailed effect of Hyl-O-glycosylation on the structure of human type I collagen at the atomic level, which may provide insights into the design and manufacture of collagenous biomaterials and the development of biomedical therapies for collagen-related diseases.

CORRELATIONS BETWEEN CLINICAL MEASUREMENT IN SYSTEMIC SCLEROSIS (SSC) AND IMMUNE RESPONSE TO TYPE I COLLAGEN (CI).

2004 ◽  
Vol 52 ◽  
pp. S286-S287
Author(s):  
A E Postlethwaite ◽  
W K Wong ◽  
J Ingels ◽  
A H Kang ◽  
P Clements ◽  
...  
Keyword(s):  
Immune Response ◽  
Systemic Sclerosis ◽  
Type I Collagen ◽  
Type I ◽  
Clinical Measurement

Evaluating Design of Abdominal Aortic Aneurysm Endografts in a Patient-Specific Model Using Computational Fluid Dynamics

2011 ◽  
Author(s):  
Polina A. Segalova ◽  
Guanglei Xiong ◽  
K. T. Rao ◽  
Christopher K. Zarins ◽  
Charles A. Taylor
Keyword(s):  
Fluid Dynamics ◽  
Blood Flow ◽  
Medical Devices ◽  
Animal Studies ◽  
Specific Model ◽  
Physical Models ◽  
Patient Specific ◽  
Attractive Alternative ◽  
Implantable Medical Devices ◽  
Abdominal Aortic

Design and evaluation of implantable medical devices often relies on benchtop testing using physical models and animal studies. Although both methods are needed, they can be costly to implement and unable to represent patient-specific physiologic conditions. Computer simulations of blood flow in patient-specific anatomies offer an attractive alternative [1].

Existing reporting guidelines for clinical trials are not completely relevant for implantable medical devices: a systematic review

2017 ◽  
Vol 91 ◽  
pp. 111-120 ◽  
Author(s):  
Anne-France Motte ◽  
Stéphanie Diallo ◽  
Hélène van den Brink ◽  
Constance Châteauvieux ◽  
Carole Serrano ◽  
...  
Keyword(s):  
Systematic Review ◽  
Clinical Trials ◽  
Medical Devices ◽  
Reporting Guidelines ◽  
Implantable Medical Devices

Combined Effect of Glycosaminoglycan and Mechanical Stimulation on the In Vitro Biomechanics of Tissue Engineered Tendon Constructs

2007 ◽  
Author(s):  
Kirsten R. C. Kinneberg ◽  
Victor S. Nirmalanandhan ◽  
Heather M. Powell ◽  
Steven T. Boyce ◽  
David L. Butler
Keyword(s):  
Tissue Engineering ◽  
Type I Collagen ◽  
Rabbit Model ◽  
The United States ◽  
Maximum Force ◽  
Attractive Alternative ◽  
Type I ◽  
Post Surgery ◽  
Scaffold Materials

Tissue engineering offers an attractive alternative to direct repair or reconstruction of injuries to tendons, ligaments and capsular structures that represent almost 45% of the 32 million musculoskeletal injuries that occur each year in the United States [1]. Mesenchymal stem cell (MSC)-seeded collagen constructs are currently being used by our group to repair tendon injuries in the rabbit model [2, 3]. Although these cell-assisted repairs exhibit 50% greater maximum force and stiffness at 12 weeks compared to values for natural repair, tissues often lack the maximum force sufficient to resist the peak in vivo forces acting on the repair site [3]. Our laboratory has previously demonstrated that in vitro construct stiffness and repair stiffness at 12 weeks post surgery are positively correlated [4]. Therefore, in an effort to further improve the repair outcome using tissue engineering, we continue our investigation of scaffold materials to create stiffer MSC-collagen constructs. Our group has recently evaluated two scaffold materials, type I collagen sponges fabricated within the Engineered Skin Lab (ESL, Shriners Hospitals for Children) by a freezing and lyophilization process with and without glycosaminoglycan (chondroitin-6-sulfate; GAG) [5] and found the ESL sponges to significantly improve biomechanical properties of the constructs compared to sponges we currently use in the lab (P1076, Kensey Nash Corporation, Exton, PA). This study also demonstrated that GAG significantly upregulates collagen type I, decorin, and fibronectin gene expression (unpublished results).

Microstructure, Spectroscopic Studies and Nanomechanical Properties of Human Cortical Bone with Osteogenesis Imperfecta

2014 ◽  
Vol 1621 ◽  
pp. 163-168
Author(s):  
Chunju Gu ◽  
Dinesh R. Katti ◽  
Kalpana S. Katti
Keyword(s):  
Fourier Transform ◽  
Osteogenesis Imperfecta ◽  
Cortical Bone ◽  
Type I Collagen ◽  
Experimental Studies ◽  
Spectroscopic Studies ◽  
Type I ◽  
Healthy Human ◽  
Electron Microscopies ◽  
Human Cortical Bone

ABSTRACTBone is a natural protein (collagen)-mineral (hydroxyapatite) nanocomposite with hierarchically organized structure. Our previous work has demonstrated orientational differences in stoichiometry of hydroxyapatite resulting from orientationally dependent collagen-mineral interactions in bone. The nature of these interactions has been investigated both through molecular dynamics simulations as well as nanomechanical and infrared spectroscopic experiments. In this study, we report experimental studies on human cortical bone with osteogenesis imperfecta (OI), a disease characterized by fragility of bones and other tissues rich in type I collagen. About 90% of OI cases result from causative variant in one of the two structural genes (COL1A1 or COL1A2) for type I procollagens. OI provides an interesting platform for investigating how alterations of collagen at the molecular level cause changes in structure and mechanics of bone. Fourier transform spectroscopy, electron microscopy (SEM), and nanomechanical experiments describe the structural and molecular differences in bone ultrastructure due to presence of diseases. Photoacoustic-Fourier transform infrared spectroscopy (PA-FTIR) experiments have been conducted to investigate the orientational differences in molecular structure of OI bone, which is also compared with that of healthy human cortical bone. Further, in situ SEM static nanomechanical testing is conducted in the transverse and longitudinal directions in the OI bone. Microstructural defects and abnormities of OI bone were ascertained using scanning electron microscopies. These results provide an insight into molecular basis of deformation and mechanical behavior of healthy human bone and OI bone.

scholarly journals Crosstalk between the α2β1 integrin and c-met/HGF-R regulates innate immunity

Blood ◽  
2008 ◽  
Vol 111 (7) ◽  
pp. 3562-3570 ◽  
Author(s):  
Karissa D. McCall-Culbreath ◽  
Zhengzhi Li ◽  
Mary M. Zutter
Keyword(s):  
Immune Response ◽  
Innate Immunity ◽  
Innate Immune Response ◽  
Cell Activation ◽  
Type I Collagen ◽  
Innate Immune ◽  
Mast Cell Activation ◽  
Type I ◽  
Matrix Metalloproteinase 1 ◽  
Α2β1 Integrin

AbstractData from several investigators suggest that the α2β1 integrin, a receptor for collagens, laminins, decorin, E-cadherin, matrix metalloproteinase-1, endorepellin, and several viruses, is required for innate immunity and regulation of autoimmune/allergic disorders. We demonstrated that the innate immune response to Listeria monocytogenes required α2β1 integrin expression by peritoneal mast cells (PMCs). Ligation of the α2β1 integrin by C1q contained in immune complexes comprised of Listeria and antibody was required for PMC activation in vitro and in vivo. However, ligation of the α2β1 integrin alone was insufficient to activate cytokine secretion, suggesting that one or more additional signals emanating from a coreceptor were required for PMC activation. Here, we demonstrate that C1q, but neither other complement proteins nor FcRγ, is required for early innate immune response to Listeria. The binding of Listeria's Internalin B (InlB) to hepatocyte growth factor receptor (HGF-R)/c-met provides the costimulatory function required for PMC activation. Either HGF or Listeria InlB bound to c-met and either C1q or type I collagen bound to α2β1 integrin stimulates PMC activation. These findings suggest that crosstalk between c-met and the α2β1 integrin may contribute to mast-cell activation in autoimmune and inflammatory disorders.

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