scholarly journals EtoGel for Intra-Articular Drug Delivery: A New Challenge for Joint Diseases Treatment

2021 ◽  
Vol 12 (2) ◽  
pp. 34
Author(s):  
Maria Chiara Cristiano ◽  
Antonia Mancuso ◽  
Elena Giuliano ◽  
Donato Cosco ◽  
Donatella Paolino ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Knee Joint ◽  
Residence Time ◽  
Three Dimensional ◽  
Typical Feature ◽  
Dimensional Network ◽  
Rheological Experiments ◽  
Drug Delivery Devices ◽  
Poloxamer Gel

Ethosomes® have been proposed as potential intra-articular drug delivery devices, in order to obtain a longer residence time of the delivered drug in the knee joint. To this aim, the conventional composition and preparation method were modified. Ethosomes® were prepared by using a low ethanol concentration and carrying out a vesicle extrusion during the preparation. The modified composition did not affect the deformability of ethosomes®, a typical feature of this colloidal vesicular topical carrier. The maintenance of sufficient deformability bodes well for an effective ethosome® application in the treatment of joint pathologies because they should be able to go beyond the pores of the dense collagen II network. The investigated ethosomes® were inserted in a three-dimensional network of thermo-sensitive poloxamer gel (EtoGel) to improve the residence time in the joint. Rheological experiments evidenced that EtoGel could allow an easy intra-articular injection at room temperature and hence transform itself in gel form at body temperature into the joint. Furthermore, EtoGel seemed to be able to support the knee joint during walking and running. In vitro studies demonstrated that the amount of used ethanol did not affect the viability of human chondrocytes and nanocarriers were also able to suitably interact with cells.

scholarly journals Development of a Biodegradable Subcutaneous Implant for Prolonged Drug Delivery Using 3D Printing

Pharmaceutics ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 105 ◽  
Author(s):  
Sarah Stewart ◽  
Juan Domínguez-Robles ◽  
Victoria McIlorum ◽  
Elena Mancuso ◽  
Dimitrios Lamprou ◽  
...  
Keyword(s):  
Drug Delivery ◽  
3D Printing ◽  
Three Dimensional ◽  
In Vitro Models ◽  
Implant Design ◽  
The Past ◽  
Drug Delivery Devices ◽  
Potential Use ◽  
Subcutaneous Implant

Implantable drug delivery devices offer many advantages over other routes of drug delivery. Most significantly, the delivery of lower doses of drug, thus, potentially reducing side-effects and improving patient compliance. Three dimensional (3D) printing is a flexible technique, which has been subject to increasing interest in the past few years, especially in the area of medical devices. The present work focussed on the use of 3D printing as a tool to manufacture implantable drug delivery devices to deliver a range of model compounds (methylene blue, ibuprofen sodium and ibuprofen acid) in two in vitro models. Five implant designs were produced, and the release rate varied, depending on the implant design and the drug properties. Additionally, a rate controlling membrane was produced, which further prolonged the release from the produced implants, signalling the potential use of these devices for chronic conditions.

Recent In Vitro and In Silico Advances in the Understanding of Intranasal Drug Delivery

2020 ◽  
Vol 26 ◽  
Author(s):  
John Chen ◽  
Andrew Martin ◽  
Warren H. Finlay
Keyword(s):  
Drug Delivery ◽  
In Silico ◽  
Local Drug Delivery ◽  
In Vivo Studies ◽  
Intranasal Drug Delivery ◽  
Nasal Sprays ◽  
In Silico Studies ◽  
Drug Delivery Devices

Background: Many drugs are delivered intranasally for local or systemic effect, typically in the form of droplets or aerosols. Because of the high cost of in vivo studies, drug developers and researchers often turn to in vitro or in silico testing when first evaluating the behavior and properties of intranasal drug delivery devices and formulations. Recent advances in manufacturing and computer technologies have allowed for increasingly realistic and sophisticated in vitro and in silico reconstructions of the human nasal airways. Objective: To perform a summary of advances in understanding of intranasal drug delivery based on recent in vitro and in silico studies. Conclusion: The turbinates are a common target for local drug delivery applications, and while nasal sprays are able to reach this region, there is currently no broad consensus across the in vitro and in silico literature concerning optimal parameters for device design, formulation properties and patient technique which would maximize turbinate deposition. Nebulizers are able to more easily target the turbinates, but come with the disadvantage of significant lung deposition. Targeting of the olfactory region of the nasal cavity has been explored for potential treatment of central nervous system conditions. Conventional intranasal devices, such as nasal sprays and nebulizers, deliver very little dose to the olfactory region. Recent progress in our understanding of intranasal delivery will be useful in the development of the next generation of intranasal drug delivery devices.

Tough Double-Network Hydrogels as Scaffolds for Tissue Engineering

2012 ◽  
pp. 213-222
Author(s):  
Jing Jing Yang ◽  
Jian Fang Liu ◽  
Takayuki Kurokawa ◽  
Nobuto Kitamura ◽  
Kazunori Yasuda ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Regeneration ◽  
Cell Types ◽  
Embryoid Bodies ◽  
Living Tissue ◽  
Double Network ◽  
Dimensional Network

Hydrogels are used as scaffolds for tissue engineering in vitro & in vivo because their three-dimensional network structure and viscoelasticity are similar to those of the macromolecular-based extracellular matrix (ECM) in living tissue. Especially, the synthetic hydrogels with controllable and reproducible properties were used as scaffolds to study the behaviors of cells in vitro and implanted test in vivo. In this review, two different structurally designed hydrogels, single-network (SN) hydrogels and double-network (DN) hydrogels, were used as scaffolds. The behavior of two cell types, anchorage-dependent cells and anchorage-independent cells, and the differentiation behaviors of embryoid bodies (EBs) were investigated on these hydrogels. Furthermore, the behavior of chondrocytes on DN hydrogels in vitro and the spontaneous cartilage regeneration induced by DN hydrogels in vivo was examined.

Effect of the drying on morphology and texture of aerogels and zirconia cryogels

MRS Advances ◽  
2019 ◽  
Vol 4 (64) ◽  
pp. 3513-3521
Author(s):  
Tzipatly A. Esquivel-Castro ◽  
Antonia Martínez-Luévanos ◽  
Luis Alfonso García-Cerda ◽  
Juan C. Contreras-Esquivel ◽  
Pascual Bartolo Pérez ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cetyltrimethylammonium Bromide ◽  
Sol Gel ◽  
Three Dimensional ◽  
Supercritical Drying ◽  
Sem Images ◽  
Texture Properties ◽  
Dimensional Network ◽  
Zirconia Aerogel

ABSTRACTDue to their excellent properties, aerogel has attracted the attention of the scientific community to use it in the biomedical area as a drug delivery system. This work reports on the synthesis and characterization of ZrO2 aerogels and cryogels obtained by the sol-gel method. The influence of different cetyltrimethylammonium bromide (CTAB) and the type of drying on structural, morphological and texture properties of ZrO2 aerogels and cryogels was investigated. SEM images reveal that a porous interconnected three-dimensional network was formed into aerogels due to supercritical drying. Zirconia aerogel sample has a specific surface area (SBET) larger than zirconia cryogels. Therefore, our results indicate that zirconia aerogel is an adequate material for applications in drug delivery systems.

Development of a nasal cast model to test medicinal nasal devices

2008 ◽  
Vol 222 (7) ◽  
pp. 1013-1022 ◽  
Author(s):  
R Hughes ◽  
J Watterson ◽  
C Dickens ◽  
D Ward ◽  
A Banaszek
Keyword(s):  
Drug Delivery ◽  
Nasal Cavity ◽  
Method Development ◽  
Test Apparatus ◽  
Nasal Drug Delivery ◽  
Nasal Airways ◽  
Cast Model ◽  
Manufacturing Technologies ◽  
Drug Delivery Devices

Bespak, a division of Consort Medical plc, and Queen's University Belfast have developed a viable and unique in-vitro testing capability for nasal drug delivery devices. The aim was to evaluate and optimize current and conceptual drug delivery devices by quantifying the deposition of drug in the various distinct regions of the nasal cavity. The development of this test apparatus employed computed tomography (CT) scan data of the human nasal cavity to construct an accurate representation of the human nasal airways. An investigation of suitable materials and manufacturing technologies was required, together with extensive analytical method development. It is possible for this technique to be further developed in an attempt to create a standardized apparatus based on nasal geometry that can be used to compare accurately deposition from drug delivery devices. This paper presents the issues encountered in the development of this test apparatus, including manufacturing and material limitations, investigation and choice of suitable materials, laboratory testing considerations, and the steps required to validate the analytical process.

Novel oral fast-disintegrating drug delivery devices with predefined inner structure fabricated by Three-Dimensional Printing

2009 ◽  
Vol 61 (3) ◽  
pp. 323-329 ◽  
Author(s):  
Deng-Guang Yu ◽  
Xia-Xia Shen ◽  
Chris Branford-White ◽  
Li-Min Zhu ◽  
Kenneth White ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Three Dimensional ◽  
Three Dimensional Printing ◽  
Dimensional Printing ◽  
Drug Delivery Devices

scholarly journals Microcapsules functionalized with neuraminidase can enter vascular endothelial cells in vitro

2014 ◽  
Vol 11 (101) ◽  
pp. 20141027 ◽  
Author(s):  
Weizhi Liu ◽  
Xiaocong Wang ◽  
Ke Bai ◽  
Miao Lin ◽  
Gleb Sukhorukov ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Endothelial Cells ◽  
Mechanical Stability ◽  
Vascular Endothelial Cells ◽  
Umbilical Vein ◽  
Three Dimensional ◽  
Endothelial Barrier ◽  
Live Cells ◽  
Umbilical Vein Endothelial Cells

Microcapsules made of polyelectrolyte multilayers exhibit no or low toxicity, appropriate mechanical stability, variable controllable degradation and can incorporate remote release mechanisms triggered by various stimuli, making them well suited for targeted drug delivery to live cells. This study investigates interactions between microcapsules made of synthetic (i.e. polystyrenesulfonate sodium salt/polyallylamine hydrochloride) or natural (i.e. dextran sulfate/poly- l -arginine) polyelectrolyte and human umbilical vein endothelial cells with particular focus on the effect of the glycocalyx layer on the intake of microcapsules by endothelial cells. Neuraminidase cleaves N -acetyl neuraminic acid residues of glycoproteins and targets the sialic acid component of the glycocalyx on the cell membrane. Three-dimensional confocal images reveal that microcapsules, functionalized with neuraminidase, can be internalized by endothelial cells. Capsules without neuraminidase are blocked by the glycocalyx layer. Uptake of the microcapsules is most significant in the first 2 h. Following their internalization by endothelial cells, biodegradable DS/PArg capsules rupture by day 5; however, there is no obvious change in the shape and integrity of PSS/PAH capsules within the period of observation. Results from the study support our hypothesis that the glycocalyx functions as an endothelial barrier to cross-membrane movement of microcapsules. Neuraminidase-loaded microcapsules can enter endothelial cells by localized cleavage of glycocalyx components with minimum disruption of the glycocalyx layer and therefore have high potential to act as drug delivery vehicles to reach tissues beyond the endothelial barrier of blood vessels.

scholarly journals Effect of Ethylenediaminetetraacetic Acid on Unsaturated Poly(Butylene Adipate-Co-Butylene Itaconate) Copolyester with Low-Melting Point and Controllable Hardness

Polymers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 611 ◽  
Author(s):  
Chin-Wen Chen ◽  
Te-Sheng Hsu ◽  
Syang-Peng Rwei
Keyword(s):  
Melting Point ◽  
Textile Industry ◽  
Ethylenediaminetetraacetic Acid ◽  
Crosslinking Agent ◽  
Three Dimensional ◽  
Uv Curing ◽  
Plastic Properties ◽  
Smart Textile ◽  
Dimensional Network

A series of copolyesters, poly(butylene adipate-co-butylene itaconate) (PBABI), was synthesized using melt polycondensation from adipic acid (AA), itaconic acid (IA), 1,4-butanediol (1,4-BDO), and ethylenediaminetetraacetic acid (EDTA). 1H-NMR, FT-IR, GPC, DSC, TGA, DMA, XRD, Shore D, and tensile test were used to systematically characterize the structural and composition/physical properties of the copolyesters. It was found that the melting point (Tm) and crystallization temperature (Tc) of the copolyesters were, respectively, between 21.1 to 57.5 °C and −6.7 to 29.5 °C. The glass transition (Tg) and the initial thermal decomposition (Td-5%) temperatures of the PBABI copolyesters were observed to be between −53.6 to −55.8 °C and 313.6 and 342.1 °C at varying ratios of butylene adipate (BA) and butylene itaconate (IA), respectively. The XRD feature peak was identified at the 2θ values of 21.61°, 22.31°, and 23.96° for the crystal lattice of (110), (020), and (021), respectively. Interestingly, Shore D at various IA ratios had high values (between 51.3 to 62), which indicated that the PBABI had soft plastic properties. The Young’s modulus and elongation at break, at different IA concentrations, were measured to be at 0.77–128.65 MPa and 71.04–531.76%, respectively, which could be attributed to a close and compact three-dimensional network structure formed by EDTA as a crosslinking agent. There was a significant bell-shaped trend in a BA/BI ratio of 8/2, at different EDTA concentrations—the ∆Hm increased while the EDTA concentration increased from 0.001 to 0.05 mole% and then decreased at an EDTA ratio of 0.2 mole%. Since the PBABI copolymers have applications in the textile industry, these polymers have been adopted to reinforce 3D air-permeable polyester-based smart textile. This kind of composite not only possesses the advantage of lower weight and breathable properties for textiles, but also offers customizable, strong levels of hardness, after UV curing of the PBABI copolyesters, making its potential in vitro orthopedic support as the “plaster of the future”.

Description and Error Evaluation of an In Vitro Knee Joint Testing System

1988 ◽  
Vol 110 (3) ◽  
pp. 238-248 ◽  
Author(s):  
J. L. Lewis ◽  
W. D. Lew ◽  
J. Schmidt
Keyword(s):  
Knee Joint ◽  
Cruciate Ligament ◽  
Three Dimensional ◽  
Experimental System ◽  
Simultaneous Recording ◽  
Testing System ◽  
Knee Ligament ◽  
Joint Biomechanics ◽  
Anterior Cruciate

An experimental system for the analysis of knee joint biomechanics is presented. The system provides for the simultaneous recording of ligament forces using buckle transducers and three-dimensional joint motion using an instrumented spatial linkage, as in vitro specimens are subjected to a variety of external loads by a pneumatic loading apparatus with associated force transducers. The system components are described, and results of an evaluation of system errors and accuracy are presented. The experimental setup has been successfully used in the analysis of normal knee ligament mechanics, as well as surgical reconstructions of the anterior cruciate ligament. The system can also be adapted to test other human or animal in vitro joints.

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