Estimation of Lipid Fractions of Blood Plasma

1955 ◽  
Vol 1 (5) ◽  
pp. 317-323 ◽  
Author(s):  
Andre C Kibrick ◽  
S J Skupp
Keyword(s):  
Fatty Acids ◽  
Oil Recovery ◽  
Volumetric Method ◽  
Total Lipids ◽  
Average Value ◽  
Esterified Fatty Acids ◽  
Normal Individuals ◽  
Lipid Fractions ◽  
Total Fatty Acids ◽  
Entire Procedure

Abstract The Allen volumetric method for plasma fat has been modified by calibration to include the percentage of olive-oil recovery. The values obtained from calibration with mercury are corrected for the 125 per cent recovery of the oil. The validity of the entire procedure has been discussed. A simple system of estimating the lipid fractions of plasma has been described. Total lipids are the total of Allen fat + (25 x lipid P). Neutral fat is Allen fat-cholesterol-cholesterol fatty acids. Total fatty acids are X neutral fat + cholesterol fatty acids + (0.64 x phospholipids). The values of total fatty acids have been compared with those obtained by oxidation with dichromate and by determining the esterified fatty acids with iron perchlorate. The values of neutral fat found in 13 apparently normal individuals are shown to range from 0 to 88 and to give an average value of 28 mg./100 ml.

scholarly journals The absorption of long-chain fatty acids from the small intestine of the sheep

1968 ◽  
Vol 22 (2) ◽  
pp. 247-254 ◽  
Author(s):  
Aileen M. Lennox ◽  
G. A. Garton
Keyword(s):  
Fatty Acids ◽  
Small Intestine ◽  
Neutral Lipids ◽  
Long Chain Fatty Acids ◽  
Linseed Meal ◽  
Total Lipids ◽  
Esterified Fatty Acids ◽  
Total Fatty Acids ◽  
Long Chain ◽  
Different Parts

1. Three sheep, each of which was fitted with a rumen cannula and with two pairs of reentrant cannulas in different parts of the small intestine, were used in this study. They were fed on dried grass cubes or hay plus linseed meal and oats: an aqueous solution of polyethylene glycol (PEG) was infused continuously into the rumen.2. Total lipids were extracted from samples of the chyme entering and leaving the different lengths of the small intestine embraced by the respective cannulas. The lipids were fractionated into unesterified fatty acids, neutral lipids and phospholipids and the contribution of each fraction to the total fatty acids was determined. The samples were also analysed for their PEG content, thus affording an index of the extent to which water had been absorbed from each particular length of intestine.3. From the above findings and a knowledge of the flow-rate of the digesta, the uptake of unesterified fatty acids and the degree of dissimilation or uptake, or of both, of esterified fatty acids was calculated.4. The results indicated that, by the time the digesta reached the ileum (i.e. the distal half of the small intestine), the uptake of fatty acids was almost complete, as was also the hydrolytic release of esterified fatty acids.5. Though there were no gross differences in the overall composition of the unesterified and esterified fatty acids in different parts of the small intestine, it appeared that C18 mono-unsaturated acid, the principal unsaturated unesterified acid, was absorbed somewhat more efficiently than were the major saturated acids (palmitic acid and stearic acid).

scholarly journals Fatty acid profile and lambs’ meat quality fed with different levels of crude glycerin replacing corn

2017 ◽  
Vol 38 (4) ◽  
pp. 2051
Author(s):  
Fabíola Cristine de Almeida Rego ◽  
Mônica Chaves Françozo ◽  
Agostinho Ludovico ◽  
Filipe Alexandre Boscaro de Castro ◽  
Marilice Zundt ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Meat Quality ◽  
Crude Glycerin ◽  
Average Weight ◽  
Total Lipids ◽  
Linear Effect ◽  
Margaric Acid ◽  
Randomized Design ◽  
Significant Difference ◽  
Total Fatty Acids

This study aimed to evaluate the Texel lambs’ meat quality fed with increasing levels of crude glycerin (0, 7, 14, and 21% of dry matter) in the diet. Thirthy-two-two non-castrated male Texel lambs were used, with initial weight of 15.9 ± 4.1 kg, using a completely randomized design with four treatments and eight repetitions, per treatment. Upon reaching the average weight of 35 kg, the animals were slaughtered. The meat chemical composition was similar among treatments (P>0.05) and total lipids and crude protein presented averages of 3.47 and 20.05 g 100g -1, respectively. No changes in pH, marbling, color, and water loss under pressure were observed. The shear force did not change among treatments and the texture can be considered soft, with average values of 5.48 kgf. There was a linear (P0.05) were observed for the attributes flavor, tenderness, juiciness, and overall acceptance among treatments. Fatty acids in higher proportions in the meat lamb were to C18:1n-9 (1298.90 mg 100g-1), C16:0 (709.07 mg 100g-1) and C18:0 (433.30 mg 100g-1), with percentages of 42.72, 23.07 and 14.35%, respectively. Significant difference according to the glycerin level in the diet was observed only for the margaric acid (C17:0) with an increasing linear effect, reaching 58.95 mg 100g-1 muscle to the group with 21% crude glycerin, corresponding to 1.77% of total fatty acids. The other fatty acids did not change, and provided a satisfactory result for conjugated linoleic acid (CLA), totaling about 6.1 mg 100 g-1 muscle, corresponding to 0.21% of total lipids. The use of glycerin in the diet for finishing lambs does not alter the characteristics of the meat, maintaining the product quality for consumption.

MENOPAUSE-INDUCED CHANGES IN LIPID FRACTIONS AND TOTAL FATTY ACIDS IN PLASMA

2001 ◽  
Vol 27 (3) ◽  
pp. 357-365 ◽  
Author(s):  
M. Maynar ◽  
G. Mahedero ◽  
I. Maynar ◽  
J. I. Maynar ◽  
I. R. Tuya ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Lipid Fractions ◽  
Total Fatty Acids ◽  
Induced Changes

scholarly journals Observations on the nature and origin of lipids in the small intestine of the sheep

1968 ◽  
Vol 22 (2) ◽  
pp. 237-246 ◽  
Author(s):  
Aileen M. Lennox ◽  
A. K. Lough ◽  
G. A. Garton
Keyword(s):  
Fatty Acids ◽  
Small Intestine ◽  
Fatty Acid ◽  
Bile Acids ◽  
Neutral Lipids ◽  
Intestinal Lumen ◽  
Total Lipids ◽  
Total Fatty Acids ◽  
Proportional Contribution ◽  
Conjugated Bile Acids

1. Total lipids were extracted from digesta obtained from the rumen, abomasum and upper small intestine (jejunum) of each of four slaughtered sheep. The lipids were fractionated into unesterified fatty acids, neutral lipids and phospholipids and the proportional contribution of each fraction to the total fatty acids was determined.2. The contribution made by phospholipids to the total fatty acids in the digesta showed a marked increase in the samples from the small intestine compared with those from the rumen and abomasum. This increase was apparently due to the presence of biliary phospholipids.3. Total lipids and conjugated bile acids were extracted from sheep bile, the lipids were fractionated and their fatty-acid composition was determined. Phospholipids predominated and these consisted mainly of phosphatidylcholine, together with some lysophosphatidylcholine.4. Both phospholipids contained significant amounts of unsaturated C18 components which could account, at least in part, for the previously reported increament to the proportion of these acids in the digesta when it enters the upper jejunum.5. The overall fatty acid compositions of the two biliary phospholipids were very similar and, in common with other naturally occurring phosphatidylcholines, the fatty acids present in position 2 of the phosphatidylcholine of bile were found to consist almost entirely of unsaturated components.6. Total lipids and conjugated bile acids were extracted from samples of digesta obtained from three sheep with cannulas in different positions in the jejunum. Analysis of the lipids indicated that biliary phospholipids, in particular phosphatidylcholine, underwent progressive hydrolysis in the intestinal lumen.7. The distribution of conjugated bile acids, unesterified fatty acids and phospholipids between the solid (particulate) and liquid (micellar) phases of the intestinal digesta was determined. These chyme constituents were, for the most part, associated with the particulate matter and thus, at any given time, it appears that only a small fraction of the total fatty acids is available for absorption in micellar form. It is suggested that the micellar solubilization of fatty acids may be facilitated by the presence of lysophosphatidylcholine.

scholarly journals Influence of diets containing different natural oils on the incorporation of [1-14C]acetate in the various lipid fractions of rat liver

1966 ◽  
Vol 20 (3) ◽  
pp. 553-559 ◽  
Author(s):  
E. P. M. Bhattathiry
Keyword(s):  
Fatty Acids ◽  
Olive Oil ◽  
Palm Oil ◽  
Neutral Lipids ◽  
Total Lipids ◽  
Stock Diet ◽  
Natural Oils ◽  
Lipid Fractions ◽  
Normal Stock

1. A comparative study was undertaken with rats on the effect of various diets (normal stock, fat-free, palm oil and olive oil) on the in vitro incorporation of [14C]acetate by the liver into cholesterol and into the fatty acids of phospholipids and neutral fats. 2. The total lipids extracted from the incubation mixtures were fractionated into acetone-precipitable and digi- tonin-precipitable portions and also into the fatty acids of neutral lipids. 3. The incorporation of [14C]acetate into the acetone-precipitable fraction and into fatty acids of neutral fats was greatest in livers of rats given the fat-free diet, followed by those of the groups given olive oil, the normal stock diet, and palm oil. Livers from the group given the fat-free diet also exhibited the highest percentage of 14C activity in the digitonin-precipitable fraction and were closely followed by the group on the normal stock diet. Compared with those of the other two groups, the livers of the groups given olive oil and palm oil showed much less activity in the digitonin- precipitable fraction. 4. The greater the amount of a specific type of fatty acid in the diet, the less was the 14C activity incorporated into that type of fattyacid in the ncutral fats of liver slices, hut this was not so with the fatty acids obtained froin the acetone-precipitahlc fraction of the lipids.

scholarly journals Analysis of the lipid fractions of coffee creamers, cream aerosols, and bouillon cubes for their health risk associated constituents

2008 ◽  
Vol 25 (No. 5) ◽  
pp. 257-264 ◽  
Author(s):  
L. Karšulínová ◽  
B. Folprechtová ◽  
M. Doležal ◽  
J. Dostálová ◽  
J. Velíšek
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Trans Fatty Acids ◽  
The Body ◽  
Fatty Acid Esters ◽  
Lipid Fractions ◽  
Hydrogenated Oils ◽  
Total Fatty Acids ◽  
Mcpd Esters ◽  
Acid Esters

Fifteen coffee creamers, 10 cream aerosols, and 5 bouillon cubes from the retail market were analysed, principally for their contents of trans-fatty acids that are known to increase the risk of coronary heart disease, and for their contents of 3-chloropropane-1,2-diol (3-MCPD) fatty acid esters that possibly have a bioaccumulation potential. The contents of trans-fatty acids in coffee creamers, cream aerosols and bouillon cubes were in the range of 0.2–32.8%, < LOD – 6.0%, and 0.5–2.1% of total fatty acids, respectively. All samples contained high levels of 3-MCPD fatty acid esters that were determined after releasing the free 3-MCPD by methanolysis. The 3-MCPD levels in coffee creamers, cream aerosols, and bouillon cubes were in the range of 130–730 µg/kg (540–4480 µg/kg fat), 50–730 µg/kg (220–2880 µg/kg fat), and 380–670 µg/kg (2650–4840 µg/kg fat), respectively. The results showed that the refined and hydrogenated oils may represent a certain risk. The highest levels of 3-MCPD esters were found in a sample of refined palm oil (4170 µg/kg). Currently, there is no information available on how these 3-MCPD esters are metabolised, to which extent they are hydrolysed or biosynthesised in the body, to which extent they are deposited in tissues, and how they influence the properties and functions of tissues (if they really do it).

Lipid changes in winter wheat seedlings (Triticum aestivum) at temperatures inducing cold hardiness

1972 ◽  
Vol 50 (12) ◽  
pp. 2401-2409 ◽  
Author(s):  
Ian A. de la Roche ◽  
C. J. Andrews ◽  
M. K. Pomeroy ◽  
Pearl Weinberger ◽  
M. Kates
Keyword(s):  
Fatty Acids ◽  
Cold Hardiness ◽  
Early Growth ◽  
Phospholipid Content ◽  
Morphological Development ◽  
Lipid Phosphorus ◽  
Total Lipids ◽  
Wheat Seedlings ◽  
Lipid Fatty Acid ◽  
Total Fatty Acids

The changes in total lipid, fatty acid, and lipid phosphorus contents were followed during germination and early growth of Rideau wheat seedlings at 24° for 72 h and 2° for 5 weeks. At both temperatures the changes in total lipids and total fatty acids were similar for seedlings at comparable stages of morphological development. In contrast, the phospholipid content of seedlings grown at 2° was considerably higher than that of seedlings grown at 24°. A marked increase in linolenic acid was also observed during growth at 2°, and this change occurred entirely in the embryo. The results indicate an increased synthesis and unsaturation of membranes in seedlings grown at 2°. The changes may contribute to the increased resistance to freezing temperatures exhibited by seedlings grown at 2°.

scholarly journals The proportion of trans monounsaturated fatty acids in serum triacylglycerols or platelet phospholipids as an objective indicator of their short-term intake in healthy men

1995 ◽  
Vol 73 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Ronald P. Mensink ◽  
Gerard Hornstra
Keyword(s):  
Fatty Acids ◽  
Control Diet ◽  
Random Order ◽  
Blood Lipid ◽  
Experimental Period ◽  
Monounsaturated Fatty Acids ◽  
Short Term ◽  
Lipid Fractions ◽  
Total Fatty Acids ◽  
Modified Diet

Unfavourable effects of trans monounsaturated fatty acid (trans-C18:1) isomers on health variables have been reported. Reports on their actual intake, however, are scarce, because of the absence in many nutrient databases of values for trans-C18:1, and the wide variation in the level of trans fatty acids between different brands of the same product. We therefore examined whether the intake of trans-C18:1 is reflected by trans-C18:1 concentrations in serum triacylglycerols or platelet phospholipids. Thirtyeight men received two diets in random order. During the first experimental period twenty men consumed a Western-type control diet for six weeks, and eighteen men consumed a modified diet in which 70% of the fat was replaced by palm oil. After a wash-out period of 3 weeks, regimens were crossed over (second experimental period). The proportion of total fatty acids from trans-C18:1 in the diet decreased from 4·7 (SEM 0·27) during the control to 2·1 (SEM 0·16) on the modified diet (P < 0·001). Trans-C18:1 in serum triacylglycerols decreased from 3·5 (SEM 0·13) to 2·8 (SEM 0·11)% (P < 0·001), and in platelet phospholipids from 1·0 (SEM 0·06) to 0·7 (SEM 0·4)% (P < 0·001). After the first experimental period trans-C18:1 in the diet correlated with trans-C18:1 in serum triacylglycerols (r 0·41; P = 0·014), and platelet phospholipids (r 0·52; P = 0·001). Also, differences in the intake between the two periods correlated with changes in the proportion of trans-C18:1 in serum triacylglycerols (r 0·56; P= 0·001) and platelet phospholipids (r 0·58; P < 0·001). These results suggest that analyses of blood lipid fractions can be used to estimate the intake, and to monitor changes in the intake, of trans-C18:1.

scholarly journals The Changes in the Lipid Composition of Mung Bean Seeds as Affected by Processing Methods

2007 ◽  
Vol 3 (5) ◽  
Author(s):  
El-Sayed Ali Abdel-Rahman ◽  
Fawzy A El-Fishawy ◽  
Mohamed A El-Geddawy ◽  
Tomas Kurz ◽  
Mohamed N El-Rify
Keyword(s):  
Fatty Acids ◽  
Lipid Composition ◽  
Mung Bean ◽  
Unsaturated Fatty Acids ◽  
Essential Fatty Acids ◽  
Saturated Fatty Acids ◽  
Gas Liquid Chromatography ◽  
Lipid Fractions ◽  
Total Fatty Acids ◽  
Tlc Analysis

This study was conducted to assess in detail the possible effects of some technological processes such as soaking, germination, cooking, soaking + cooking, and germination + cooking on the lipid composition of mung bean seeds of Giza 1 variety. TLC analysis of mung bean lipids showed that the phospholipids and triglycerides recorded the highest percentage among lipid fractions (32.26 and 30.10%), while the 1,3 diglycerides constituted the least percentage (2.80%) in mung bean seeds. The soaking, germination and cooking processes caused a decrease in the phospholipids, triglycerides and hydrocarbons accompanied with an increase in monoglycerides, 1,2-(2,3)-diglycerides, sterols and free fatty acids. Eleven fractions were separated from phospholipids class of the studied samples; seven of these fractions were identified. The major component of phospholipids was phosphatidyl choline, amounting to 21.30, 17.84, 16.21, 13.87, 13.20 and 11.47% of the total phospholipids in raw, soaked, germinated, raw-cooked, soaked-cooked and germinated-cooked mung bean seeds, respectively. Gas liquid chromatography of the total lipids of mung bean seeds showed that the unsaturated fatty acids represented 69.58, 64.35, 63.3, 63.16, 61.84 and 61.12%, while the levels of saturated fatty acids were low being 30.37, 34.05, 35.66, 34.64, 37.93 and 38.75% of the total fatty acids in raw, soaked, germinated, raw-cooked, soaked-cooked and germinated-cooked, respectively. The total essential fatty acids (linoleic and linolenic) represented the highest proportion of fatty acids (50.10% of the total fatty acids).

scholarly journals Comparative Analysis of Lipids and Fatty Acids in Beaked Redfish Sebastes mentella Travin, 1951 Collected in Wild and in Commercial Products

2022 ◽  
Vol 10 (1) ◽  
pp. 59
Author(s):  
Svetlana A. Murzina ◽  
Viktor P. Voronin ◽  
Tatjana R. Ruokolainen ◽  
Dmitrii V. Artemenkov ◽  
Alexei M. Orlov
Keyword(s):  
Fatty Acids ◽  
Quality Index ◽  
Total Lipids ◽  
Commercial Products ◽  
Lipid Quality ◽  
Esterified Fatty Acids ◽  
Water Fish ◽  
In The Wild ◽  
Sebastes Mentella ◽  
Quality Indexes

The lipid and fatty acid profile of muscles in beaked redfish, caught and fixed in the wild versus specimens from food supermarkets (“commercial”), were evaluated, as well as the health implications of this popular food for its consumers based on the calculation of nutritional quality indexes. The contents of the total lipids (TLs), total phospholipids (PLs), monoacylglycerols (MAGs), diacylglycerols (DAGs), triacylglycerols (TAGs), cholesterol (Chol), Chol esters, non-esterified fatty acids (NEFAs), and wax esters were determined by HPTLC; the phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), and lysophosphatidylcholine (LysoPC) were determined by HPLC; and fatty acids of total lipids were determined using GC. The TL content was higher in commercial products due to DA and NEFAs, among PL fractions the content of LysoPC was also higher. The results indicated multidirectional processes of slight degradation of lipids in commercial products in comparison to wild. The flesh lipid quality index was lower due to EPA and DHA in commercial specimens while the index of thrombogenicity was significantly higher. The differences in the quantities of lipid classes between muscle biopsy regions in fish apparently corroborate the morphology and physiology of deep-water fish.

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