scholarly journals Reassessment of Dyfrolomyces and Four New Species of Melomastia from Olive (Olea europaea) in Sichuan Province, China

2022 ◽  
Vol 8 (1) ◽  
pp. 76
Author(s):  
Wen-Li Li ◽  
Sajeewa S. N. Maharachchikumbura ◽  
Ratchadawan Cheewangkoon ◽  
Jian-Kui Liu

Pleurotremataceae species are saprobes on decaying wood in terrestrial, mangrove, and freshwater habitats. The generic boundary of the family has traditionally been based on morphology. All genera of Pleurotremataceae have a high degree of morphological overlap, of which the generic circumscription of Melomastia and Dyfrolomyces has not been well resolved. Thus, the delimitation of genera has always been challenging. Melomastia traditionally differs from Dyfrolomyces in having 2-septate, oblong, with obtuse-ends ascospores. These main characteristics have been used to distinguish Melomastia from Dyfrolomyces for a long time. However, the above characteristics sometimes overlap among Dyfrolomyces and Melomastia species. Based on the morphology and multigene phylogeny with newly obtained data, we synonymized Dyfrolomyces under Melomastia following up-to-date results. Four novel species (i.e., Melomastia fusispora, M. oleae, M. sichuanensis and M. winteri) collected from the dead branches of Olea europaea L. in Chengdu Olive Base, Sichuan Province in China are introduced based on detailed morphological characterization and phylogenetic analyses of sequences based on nuclear ribosomal (LSU and SSU) and protein-coding gene (tef1-α). The 11 new combinations proposed are Melomastia aquatica (= Dyfrolomyces aquaticus), M. chromolaenae (= D. chromolaenae), M. distoseptata (= D. distoseptatus), M. mangrovei (= D. mangrovei), M. marinospora (= D. marinosporus), M. neothailandica (= D. neothailandicus), M. phetchaburiensis (= D. phetchaburiensis), M. sinensis (= D. sinensis), M. thailandica (= D. thailandica), M. thamplaensis (= D. thamplaensis) and M. tiomanensis (= D. tiomanensis).

2020 ◽  
Vol 25 (11) ◽  
pp. 2003-2017
Author(s):  
Leiva Sergio ◽  
Moraes Gilberto J. De

Damages produced by eriophyid mites to Olea europaea subsp. europaea cv. arauco have been known for many years. In a previous publication, the presence of Aceria oleae (Nalepa) and Oxycenus maxwelli, (Keifer) in Argentina was reported, but the species was not definitively identified. In the past five years, damage caused by this mite to leaves, flowers and fruits in many production areas was very high, leading to the need for further investigations about this mite, the most numerous mite species on this plant in Argentina. The present paper is divided in three parts: a) a morphological characterization of A. oleae based on specimens collected in Argentina, with the use of SEM; b) a study of its life cycle; and c) an illustration of its damage to leaves and fruits. The morphological study led to the confirmation of the identity of the species and to the determination of morphological aspects not previously mentioned in the literature. Life cycle and damage were observed to be similar to what has been previously mentioned in the literature for the same mite in other countries.


Genes ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1306
Author(s):  
Shiwen Xu ◽  
Yunfei Wu ◽  
Yingqi Liu ◽  
Ping Zhao ◽  
Zhuo Chen ◽  
...  

Pentatomoidea is the largest superfamily of Pentatomomorpha; however, the phylogenetic relationships among pentatomoid families have been debated for a long time. In the present study, we gathered the mitogenomes of 55 species from eight common families (Acanthosomatidae, Cydnidae, Dinidoridae, Scutelleridae, Tessaratomidae, Plataspidae, Urostylididae and Pentatomidae), including 20 newly sequenced mitogenomes, and conducted comparative mitogenomic studies with an emphasis on the structures of non-coding regions. Heterogeneity in the base composition, and contrasting evolutionary rates were encountered among the mitogenomes in Pentatomoidea, especially in Urostylididae, which may lead to unstable phylogenetic topologies. When the family Urostylididae is excluded in taxa sampling or the third codon positions of protein coding genes are removed, phylogenetic analyses under site-homogenous models could provide more stable tree topologies. However, the relationships between families remained the same in all PhyloBayes analyses under the site-heterogeneous mixture model CAT + GTR with different datasets and were recovered as (Cydnidae + (((Tessaratomidae + Dinidoridae) + (Plataspidae + Scutelleridae)) + ((Acanthosomatidae + Urostylididae) + Pentatomidae)))). Our study showed that data optimizing strategies after heterogeneity assessments based on denser sampling and the use of site-heterogeneous mixture models are essential for further analysis of the phylogenetic relationships of Pentatomoidea.


Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1544 ◽  
Author(s):  
Valeria De Matteis ◽  
Loris Rizzello ◽  
Chiara Ingrosso ◽  
Eva Liatsi-Douvitsa ◽  
Maria Luisa De Giorgi ◽  
...  

The green synthesis of nanoparticles (NPs) is currently under worldwide investigation as an eco-friendly alternative to traditional routes (NPs): the absence of toxic solvents and catalysts make it suitable in the design of promising nanomaterials for nanomedicine applications. In this work, we used the extracts collected from leaves of two cultivars (Leccino and Carolea) belonging to the species Olea Europaea, to synthesize silver NPs (AgNPs) in different pH conditions and low temperature. NPs underwent full morphological characterization with the aim to define a suitable protocol to obtain a monodispersed population of AgNPs. Afterwards, to validate the reproducibility of the mentioned synthetic procedure, we moved on to another Mediterranean plant, the Laurus Nobilis. Interestingly, the NPs obtained using the two olive cultivars produced NPs with different shape and size, strictly depending on the cultivar selected and pH. Furthermore, the potential ability to inhibit the growth of two woman cancer cells (breast adenocarcinoma cells, MCF-7 and human cervical epithelioid carcinoma, HeLa) were assessed for these AgNPs, as well as their capability to mitigate the bacteria concentration in samples of contaminated well water. Our results showed that toxicity was stronger when MCF-7 and Hela cells were exposed to AgNPs derived from Carolea obtained at pH 7 presenting irregular shape; on the other hand, greater antibacterial effect was revealed using AgNPs obtained at pH 8 (smaller and monodispersed) on well water, enriched with bacteria and coliforms.


2020 ◽  
Vol 6 (3) ◽  
pp. 174
Author(s):  
Milan C. Samarakoon ◽  
Sajeewa S. N. Maharachchikumbura ◽  
Jian-Kui (Jack) Liu ◽  
Kevin D. Hyde ◽  
Itthayakorn Promputtha ◽  
...  

Amphisphaeriaceous taxa (fungi) are saprobes on decaying wood in terrestrial, mangrove, and freshwater habitats. The generic boundaries of the family have traditionally been based on morphology, and the delimitation of genera has always been challenging. Amphisphaeria species have clypeate ascomata and 1-septate ascospores and a coelomycetous asexual morph. Lepteutypa is different from Amphisphaeria in having eutypoid stromata and more than 1-septate ascospores. These main characters have been used for segregation of Lepteutypa from Amphisphaeria for a long time. However, the above characters are overlapping among Amphisphaeria and Lepteutypa species. Therefore, here we synonymized Lepteutypa under Amphisphaeria based on holomorphic morphology and multigene phylogeny. Further, our cluster analysis reveals the relationship between seven morphological traits among Amphisphaeria/Lepteutypa species and suggests those morphologies are not specific to either genus. Three new species (i.e., Amphisphaeria camelliae, A. curvaticonidia, and A. micheliae) are introduced based on morphology and LSU-ITS-RPB2-TUB2 phylogenies. Furthermore, the monotypic genus Trochilispora, which had been accepted in Amphisphaeriaceae, is revisited and synonymized under Hymenopleella and placed in Sporocadaceae.


2018 ◽  
Vol 77 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Ergun Kaya ◽  
Recep Vatansever ◽  
Ertugrul Filiz

AbstractThe olive tree (Olea europaeaL.) is one of the major cultivated species in the world, and Mediterranean countries produce about 90% of world cultivated olives. In this study, the genetic relationship of seven Turkish olive cultivars was investigated using non-codingtrnL-Fregions in chloroplastic genome. Cultivars demonstrated a similar sequence length of 330-340 bp with an average 35.26% G+C content. Variable (polymorphic/segregating), parsimony informative and total numbers of the insertion or the deletion of bases in the DNA (indel sites) were 4, 3, and 28, respectively. Nucleotide diversities π and θ were found as 0.00631 and 0.00644 respectively, while Tajima’s D was −0.786. cpDNAtrnL-Fregions of sequenced Turkish olive cultivars had a low level of genetic variations, and these non-coding regions were strictly conserved in all analyzed cultivars. Geographically distant shared more sequence similarities than relatively close cultivars. The phylogenetic analyses indicated that the biogeographic distribution of cultivars does not demonstrate any association inferring cultivar source. These results indicate the possibility of germplasm exchanges among countries or that some indel mutations contribute to variations of the Turkish olive gene pool. Thus, the authorities should develop the necessary programs to preserve the purity of native germplasms.


2020 ◽  
Vol 116 (2) ◽  
pp. 311
Author(s):  
Rachid BOUKHARI ◽  
Abdelkader AMEUR AMEUR ◽  
Hocine INNAL ◽  
Semir Bechir Suheil GAOUAR

<p>Olive (<em>Olea europaea</em> L.) resources in Algeria are very little investigated. In fact, a total of 60 denominations have been the subject of characterization studies and they are cited in the bibliography but only 36 varieties are described and listed in the catalog of Algerian varieties of olive tree. In this work, a study on the diversity by mean of a field survey followed by morphological characterization, an estimate of the Shannon diversity index of a set of denominations collected in central and eastern of Algeria were carried out.<strong> </strong>The survey allowed us to note the existence of 33 denominations never mentioned in the bibliography. Morphological characterization based on the characteristics of tree, fruit and endocarp allowed us to a morphological description of 23 denominations. The Principal Component Analysis, Multiple Correspondence Analysis and Ascending Hierarchical Classification analysis allowed us to classify them into a few groups. The value of the relative diversity of all denominations is slightly below the average (0.42).<strong> </strong>The results obtained in this work provide very useful information on certain morphological characteristics of the studied denominations and indicate the critical conditions in which several denominations are found, which constitutes a great risk of genetic erosion.</p>


Phytotaxa ◽  
2017 ◽  
Vol 308 (2) ◽  
pp. 206 ◽  
Author(s):  
JUNFU LI ◽  
RAJESH JEEWON ◽  
ZONGLONG LUO ◽  
RUNGTIWA PHOOKAMSAK ◽  
DARBHE J. BHAT ◽  
...  

Fusiconidium mackenziei sp. nov. and Fusiconidium aquaticum sp. nov., isolated from a dead branch of Clematis vitalba in Italy and a submerged decaying wood in China respectively, are described and illustrated. The new taxa are characterized by having rhexolytic conidial secession, percurrently proliferating conidiophores, with enteroblastic conidiogenous cells and acrogenous, pale brown to dark brown, verruculose, fusiform, euseptate conidia. Fusiconidium is introduced as a novel genus to accommodate these novel species base on morphologically distinct from related asexual genera within the Dothideomycetes and multigene phylogenetic analyses. Based on multigene phylogenetic analyses, Fusiconidium is referred to the family Melanommataceae (Pleosporales).


Phytotaxa ◽  
2016 ◽  
Vol 267 (1) ◽  
pp. 61 ◽  
Author(s):  
HONG-YAN SU ◽  
ZONG-LONG LUO ◽  
XIAO-YING LIU ◽  
XI-JUN SU ◽  
DIAN-MING HU ◽  
...  

Lentithecium cangshanense sp. nov. (Lentitheciaceae, Dothideomycetes), was found on submerged decaying wood in a freshwater stream in Yunnan Province, China. The species is characterized by its black, semi-immersed to superficial, globose ascomata, cylindrical or obclavate, short pedicellate, bitunicate asci and bi-seriate, fusiform, 1-septate, yellowish to brown ascospores. Phylogenetic analyses of combined LSU, SSU and RPB2 sequence data show that L. cangshanense belongs in the family Lentitheciaceae, order Pleosporales and is a distinct species in the genus. The new species is introduced with an illustrated account and compared with morphologically and phylogenetically similar species.


2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Guodong Rao ◽  
Jianguo Zhang ◽  
Xiaoxia Liu ◽  
Chunfu Lin ◽  
Huaigen Xin ◽  
...  

AbstractOlive (Olea europaea L.) is internationally renowned for its high-end product, extra virgin olive oil. An incomplete genome of O. europaea was previously obtained using shotgun sequencing in 2016. To further explore the genetic and breeding utilization of olive, an updated draft genome of olive was obtained using Oxford Nanopore third-generation sequencing and Hi-C technology. Seven different assembly strategies were used to assemble the final genome of 1.30 Gb, with contig and scaffold N50 sizes of 4.67 Mb and 42.60 Mb, respectively. This greatly increased the quality of the olive genome. We assembled 1.1 Gb of sequences of the total olive genome to 23 pseudochromosomes by Hi-C, and 53,518 protein-coding genes were predicted in the current assembly. Comparative genomics analyses, including gene family expansion and contraction, whole-genome replication, phylogenetic analysis, and positive selection, were performed. Based on the obtained high-quality olive genome, a total of nine gene families with 202 genes were identified in the oleuropein biosynthesis pathway, which is twice the number of genes identified from the previous data. This new accession of the olive genome is of sufficient quality for genome-wide studies on gene function in olive and has provided a foundation for the molecular breeding of olive species.


2019 ◽  
Vol 51 (5) ◽  
Author(s):  
Muhammad Azhar Iqbal ◽  
Ishfaq Ahmad Hafiz ◽  
Nadeem Akhtar Abbasi ◽  
Muhammad Kausar Nawaz Shah

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