Thrombocytopenia as Type 1 ROP Biomarker: A Longitudinal Study

This study aimed to prospectively evaluate the association between the appearance and evolution of retinopathy of prematurity (ROP) and selected blood parameters, focusing on platelets count. In total, 157 preterm consecutive babies screened for ROP were included and classified in: ROP necessitating treatment (group ROP1), ROP regressed without therapy (group ROP2) and no ROP (group no-ROP), divided in two phases for each group depending on gestational age. Blood parameters were weekly gathered and referred to postmenstrual age, ROP severity and phase. Platelet count mean values were statistically lower (p < 0.001) during both phases in ROP1 group (179 × 109/L vs. 213 × 109/L in phase 1 and 2, respectively) vs. other groups (ROP2: 286 × 109/L vs. 293 × 109/L; no ROP: 295 × 109/L vs. 313 × 109/L). Platelet count at birth <181 × 109 was statistically associated with Type 1 ROP development and evolution (sensibility = 76.47%, 95% confidence interval 60.0–87.6; specificity = 66.12%, 95% confidence interval 57.3–73.9). In ROP 1 group, a platelets count mean value “spike” (392.6 × 109/L) was documented at 36 weeks of corrected gestational age, preceding the need for treatment performed at a median of 38.1 ± 3.2 weeks. Early birth thrombocytopenia is confirmed as a biomarker of development and progression of ROP requiring treatment. The increase of platelets count at 35–37 weeks of corrected gestational age can be considered a possible clinical biomarker anticipating Type 1 ROP progression in preterm infants.

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Incidence and Characteristics of Infants with Retinopathy of Prematurity in Croatia

Integrative Pediatrics and Child Care ◽

10.18314/ipcc.v1i1.1258 ◽

2018 ◽

Vol 1 (1) ◽

pp. 35-45

Author(s):

Mirjana Vucinovic ◽

Ljubo Znaor ◽

Ana Vucinovic ◽

Vesna Capkun ◽

Julijana Bandic

Keyword(s):

Risk Factors ◽

Intensive Care Unit ◽

Intensive Care ◽

Neonatal Intensive Care Unit ◽

Gestational Age ◽

Retinopathy Of Prematurity ◽

Small For Gestational Age ◽

Neonatal Intensive Care ◽

Type 1 Rop

PURPOSE: To study the incidence of retinopathy of prematurity (ROP) in a neonatal intensive care unit in Croatia and obtain information on risk factors associated with ROP. There have been limited studies on ROP in Croatia where the screening for ROP and its treatment is still insufficient and not introduced in many intensive care units. MATERIAL AND METHODS: This retrospective study included 247 premature infants admitted to the neonatal intensive care unit of University Hospital Split, over a 5-year period between January 2012, and December 2016. In this paper the relationship between clinical risk factors and the development of ROP was analyzed. RESULTS: The overall incidence for ROP was 23,9 % (59 infants), for Type 1 ROP was 9,3% (23 infants); for Type 2 ROP was 14,6% (36 infants). Median gestational age (GA) and birthweight (BW) were significantly lower among infants with ROP versus those without ROP (29: 23-34 vs. 31: 23-34,p<0,001 and 1,180:630-2,000 vs. 1485:590-2000, p<0,001 respectively). Multivariate analysis showed that only BW (p=0,029) and small for gestational age (SGA) (p=0,045) predicted the development of ROP. CONCLUSION: Birth weight and small for gestational age were the most significant risk factors for developing ROP. In comparison with studies from highly developed countries, infants with a much wider range of gestational age and birth weights are developing Type 1 ROP.

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Low Gestational Age Is the Strongest Predictor for Severe Retinopathy of Prematurity and Adverse Outcomes at Two-Year Follow-Up in a Low Incidence Setting

Journal of Child Science ◽

10.1055/s-0037-1604476 ◽

2017 ◽

Vol 07 (01) ◽

pp. e106-e109

Author(s):

Stefano Nobile ◽

Linda Bordignon ◽

Anna Peretti ◽

Virgilio Carnielli

Keyword(s):

Bronchopulmonary Dysplasia ◽

Gestational Age ◽

Univariate Analysis ◽

Adverse Outcomes ◽

Neurological Development ◽

Neurodevelopmental Outcomes ◽

Surfactant Administration ◽

Low Incidence ◽

Type 1 Rop

AbstractWe aimed to determine the risk factors for type 1 ROP and outcomes at 2 years of corrected age among preterms born < 30 weeks of gestational age (GA) in a low-incidence setting. A cohort study with 447 infants was conducted. Those who underwent laser therapy for type 1 ROP were compared with controls by univariate and multivariate analysis. Neurological development was assessed by Bayley scales. Using univariate analysis, gestational age, sepsis, necrotizing enterocolitis, and insulin administration were found to be associated with type 1 ROP. Only GA remained significant with logistic regression. Infants with type 1 ROP had worse visual and neurodevelopmental outcomes at 2 years. Predictors of impaired neurodevelopment were type 1 ROP, surfactant administration, and bronchopulmonary dysplasia. Low GA was found to be a strong predictor of type 1 ROP, which was associated (along with surfactant administration and bronchopulmonary dysplasia) with worse neurological development.

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Time to regain birth weight - a marker to predict the severity of retinopathy of prematurity?

BMC Pediatrics ◽

10.1186/s12887-021-03027-x ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Ajay Anvekar ◽

Sam Athikarisamy ◽

Shripada Rao ◽

Andy Gill ◽

Elizabeth Nathan ◽

...

Keyword(s):

Preterm Infants ◽

Gestational Age ◽

Retinopathy Of Prematurity ◽

Oxygen Therapy ◽

Control Groups ◽

Gestational Age At Birth ◽

And Control ◽

Type 1 Rop ◽

Age At Birth

Abstract Background Poor weight gain in the first few weeks of life has been studied as a predictor of retinopathy of prematurity (ROP). Our aim was to assess whether time taken to regain birthweight (BW) be used as an additional marker to identify infants with type 1 ROP. Methods In this retrospective study, preterm infants (< 27 weeks gestational age at birth) born during the period from 1/1/2010–31/12/2015 at a tertiary neonatal intensive care unit in Australia were included. Twenty-seven preterm infants with Type 1 ROP were identified. Controls (No ROP or ROP other than type 1) were matched with cases on gestational age at birth and BW (1:4 ratio). Data were collected from the database and medical records. Results The median (IQR) gestational age for Type 1 ROP and control groups were 24 (24–26) and 25 (24–26) weeks respectively and median (IQR) BW for Type 1 ROP and control groups were 675 (635–810) and 773 (666–884) grams respectively. Preterm infants with Type 1 ROP were more likely to be small for gestational age (SGA) (18.5% vs 3.7%, p = 0.015) and had increased weeks on oxygen therapy (median 11.9 vs 9.1, p = 0.028). Time to regain BW was longer in preterm infants with type 1 ROP than controls but did not reach statistical significance (median 9 vs 7 days, OR 1.08, 95% CI 1.00–1.17, p = 0.059) adjusted for SGA and duration of oxygen therapy. The area under the curve from the time to regain BW model with adjustment for SGA and duration of oxygen therapy was 0.73 (95% CI 0.62–0.83). Conclusion We hypothesize that time to regain BW has potential to aid prediction of Type 1 ROP and this warrants further investigation in a larger prospective study.

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Fluorescein Angiography of Persistent Incomplete Vascularization of the Retina After Bevacizumab Therapy in Retinopathy of Prematurity

Journal of VitreoRetinal Diseases ◽

10.1177/2474126419850980 ◽

2019 ◽

Vol 3 (5) ◽

pp. 304-308 ◽

Cited By ~ 1

Author(s):

Xihui Lin ◽

Sagar B. Patel ◽

Alice Y. Zhang ◽

Daniel A. Brill ◽

Kim H. Le ◽

...

Keyword(s):

Fluorescein Angiography ◽

Gestational Age ◽

Retinopathy Of Prematurity ◽

Case Series ◽

Intravitreal Bevacizumab ◽

Peripheral Retina ◽

Medical Systems ◽

Retrospective Case ◽

Type 1 Rop

Purpose: This study evaluates the vascular development of premature infants at 50 weeks postconception after treatment with intravitreal bevacizumab for type 1 retinopathy of prematurity (ROP). Methods: A retrospective case series was conducted on type 1 ROP neonates with a high risk of general anesthesia complications. Patients were treated with bevacizumab between February 2013 and February 2016. At 50 weeks’ gestational age, all patients were imaged with RetCam (Clarity Medical Systems, Inc) fundus photographs and widefield fluorescein angiography (FA). Results: There were 4 boys and 7 girls with an average gestational age of 24.8 weeks and weight of 734.3 grams at birth. The estimated gestational age at injection was 37.1 weeks. Examination under anesthesia with FA was performed at a mean of 61 weeks posttreatment. Nineteen of 20 eyes (95%) of 11 infants had incomplete vascularization and peripheral nonperfusion on imaging. FA showed new vessels with increased tortuosity, microvascular abnormalities, and leakage past the original ridge. These 19 patients were treated with confluent ablative photocoagulation to the peripheral nonperfused retina. Patients were followed for a mean of 73.3 weeks after birth. None required incisional surgery. Conclusions: Patients with type 1 ROP treated with bevacizumab may have delayed vascularization of the peripheral retina and even late recurrence of active disease. Clinicians should regularly monitor patients after bevacizumab for potential neovascular complications.

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Optimization of a Density Gradient Centrifugation Protocol for Isolation of Peripheral Blood Mononuclear Cells

Acta Medica Marisiensis ◽

10.2478/amma-2018-0011 ◽

2018 ◽

Vol 64 (2) ◽

pp. 83-90 ◽

Cited By ~ 2

Author(s):

Georgiana Mihaela Şerban ◽

Ion Bogdan Mănescu ◽

Doina Ramona Manu ◽

Minodora Dobreanu

Keyword(s):

Mononuclear Cells ◽

Density Gradient Centrifugation ◽

Gradient Centrifugation ◽

Phase 1 ◽

Sample Volume ◽

Mean Value ◽

Phase 2 ◽

Cell Recovery ◽

Peripheral Blood Mononuclear ◽

Mean Values

Abstract Objective: Peripheral blood mononuclear cells (PBMC) are extremely important in the body’s immune response. Their isolation represents a major step in many immunological experiments. In this two phase study, we aimed to establish an optimum protocol for PBMC isolation by density-gradient centrifugation. Methods: During Phase-1, we compared two commercially available PBMC isolation protocols, Stemcell Technologies (ST) and Miltenyi Biotec (MB), in terms of PBMC recovery and purity. Twelve blood samples were assigned to each protocol. Each sample was divided in three subsamples of 1ml, 2ml and 3ml in order to assess the influence of blood sample volume on isolation performance. During Phase-2, a hybrid protocol was similarly tested, processing six blood samples. Additionally, we performed a flow cytometric analysis using an Annexin-V/Propidium-Iodide viability staining protocol. Results: Phase-1 results showed that, for all subsample volumes, ST had superior PBMC recovery (mean values: 56%, 80% and 87%, respectively) compared to MB (mean values: 39%, 54% and 43%, respectively). However, platelet removal was significantly higher for MB (mean value of 96.8%) than for ST (mean value of 75.2%). Regarding granulocyte/erythrocyte contamination, both protocols performed similarly, yielding high purity PBMC (mean values: 97.3% for ST and 95.8% for MB). During Phase-2, our hybrid protocol yielded comparable results to MB, with an average viability of 89.4% for lymphocytes and 16.9% for monocytes. Conclusions: ST yields higher cell recovery rates and MB excels at platelet removal, while the hybrid protocol is highly similar to MB. Both cell recovery and viability increase with blood sample volume.

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The importance of timely ophthalmologic examination in preterm infants at risk of retinopathy occurrence

Vojnosanitetski pregled ◽

10.2298/vsp1209765k ◽

2012 ◽

Vol 69 (9) ◽

pp. 765-770

Author(s):

Sanja Knezevic ◽

Nadezda Stojanovic ◽

Ana Oros ◽

Jasmina Knezevic

Keyword(s):

Body Weight ◽

Birth Weight ◽

Gestational Age ◽

Mean Value ◽

Severe Form ◽

Healthy Children ◽

Ophthalmological Examination ◽

Mean Values ◽

Severe Stage ◽

The Mean

Background/Aim. Retinopathy of prematurity (ROP) is a multifactorial disease in premature infants. The aim of this study was to determine the incidence of ROP in children treated at the Center of Neonatology, Pediatric Clinic, Clinical Center in Kragujevac, Serbia. Methods. The study covered all children with birth weight below 2,000 g and/or gestational age below the 37th week, who from June 2006 to December 2009 underwent ophthalmological examination for ROP. The results of fundoscopy were classified in accordance with the International Classification of ROP. The treatment of infants and those with ROP was conducted in accordance with the early treatment of ROP study recommendations. We analyzed gestational age, birth weight and postconceptional age in two groups: healthy infants and those with severe form of ROP. Statistical analysis was performed using the SPSS 16. Results. A total of 478 children met the criteria of screening for ROP. Severe stage of ROP, which required laser treatment, had 102 (21.3%) children. Out of the infants with severe ROP 14 (13.7%) of the infants with APD had aggressive posterior disease, while two (0.4%) remained blind. The differences in the mean values of gestational age between the healthy and the children with severe form of the disease were statistically significant (p < 0.0005). The mean value of gestational age for the healthy children was 33.33 ? 2.28 weeks and for the seek infants 30.66 ? 2.79 weeks. The mean value of the weight in healthy children was 1.981 ? 407 g, and in sick children 1.535 ? 434 g which was statistically significant (p < 0.0005). Multivariate binary logistic regression showed that the occurrence of the disease depends on body weight and gestational age. Conclusion. The incidence of severe forms of ROP was 21.3%. Aggressive form of ROP was present in 13.7% of the children. The cut-off value for body weight was 1.740 g, and for gestational age 32.5 weeks.

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CORRELATIONS OF THE USUAL BLOOD PARAMETERS WITH THE INCIDENCE OF DENTAL CARIES IN A GROUP OF PATIENTS WITH CHRONIC ALCOHOLIC HEPATITIS

Romanian Medical Journal ◽

10.37897/rmj.2017.3.4 ◽

2017 ◽

Vol 64 (3) ◽

pp. 201-206

Author(s):

Daniela G. Bădiţa ◽

◽

Iulia I. Stănescu ◽

Andra E. Balcangiu-Stroescu ◽

Bogdan Calenic ◽

...

Keyword(s):

Chronic Hepatitis ◽

Platelet Count ◽

Dental Caries ◽

Blood Cell ◽

Red Blood Cell ◽

Hemoglobin Concentration ◽

Blood Parameters ◽

Chronic Alcoholic ◽

Mean Values ◽

Total Hemoglobin

Objectives. To investigate the correlations between the frequency of dental caries, assessed as cariogenic index, and a series of blood parameters in patients diagnosed with chronic hepatitis of alcoholic etiology. Materials and method. The study was conducted on a total of 25 patients diagnosed with chronic hepatitis of alcoholic etiology. The following blood parameters were determined: blood count (erythrocyte count, total hemoglobin, hematocrit, mean red blood cell volume, mean red blood cell hemoglobin, mean erythrocyte hemoglobin concentration), platelet count, white blood cell count (leukocyte count, neutrophils ratio, lymphocytes ratio, monocytes ratio, eosinophils ratio, basophils ratio) and glucose, urea and creatinine concentrations. Results. The mean values were: cariogenic index 1.8±0.96, erythrocytes 4.66±0.51x106/mm3, total hemoglobin concentration 14.67±1.26 g/dL, hematocrit 44.45±3.75%, mean erythrocyte volume 95.6±6.03 fL, mean erythrocyte hemoglobin 31.6±1.86 pg, mean erythrocyte hemoglobin concentration 33.01±1.02 g/dL, platelet count 2.7±0.61x105/mm3, leukocytes 8,812±2,065.66/mm3, neutrophils ratio 63.32±8.75%, lymphocytes ratio 26.32±8.48 %, monocytes ratio 7.99±2.02 %, eosinophils ratio 1.87±1.6%, basophils ratio 0.5±0.31%, glycemia 91.52±8.38 mg/dL, urea concentration 32.12±8.44 mg/dL and creatinine 0.92±0.19 mg/dL. Conclusions. We have been able to establish statistically significant correlations between the cariogenic index and total hemoglobin, hematocrit, mean erythrocyte hemoglobin concentration and glycemia.

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Mid-arm circumference and mid-arm/head circumference ratio in term newborns

Sao Paulo Medical Journal ◽

10.1590/s1516-31802004000200004 ◽

2004 ◽

Vol 122 (2) ◽

pp. 53-59 ◽

Cited By ~ 4

Author(s):

Bettina Barbosa Duque Figueira ◽

Conceição Aparecida de Mattos Segre

Keyword(s):

Birth Weight ◽

Gestational Age ◽

Head Circumference ◽

Cross Sectional Study ◽

Mean Value ◽

Cross Sectional ◽

Mean Values ◽

Arm Circumference ◽

Term Newborns ◽

The Mean

CONTEXT: Mid-arm circumference of the newborn is strongly associated with birth weight and is a very good indicator of low and insufficient birth weight. However, there are few Brazilian studies on the relationship between mid-arm and head circumferences and, thus, this does not form part of the routine evaluation for newborns. OBJECTIVES: To establish the mid-arm circumference and mid-arm/head circumference ratio in a population of term newborns. TYPE OF STUDY: Cross-sectional study carried out between June 1997 and August 1999. SETTING: Hospital Maternidade Leonor Mendes de Barros, São Paulo. PARTICIPANTS: Term newborns (66 males and 65 females) of appropriate growth for gestational age, whose mothers were healthy, were included in the study. MAIN MEASUREMENTS: Arm circumference, arm circumference/head circumference ratio, birth weight and gestational age were measured within 48 hours of birth. Data were considered significant when p < 0.01. RESULTS: The mean values for the mid-arm circumference were 10.76 cm (standard deviation, SD = 0.68) for females and 10.76 (SD = 0.81) for males. The mean value for the mid-arm/head circumference ratio was 0.31 (SD = 0.02) for both sexes. Mid-arm circumference values were significantly related to birth weight and gestational age, whereas mid-arm/head circumference ratio was related only to birth weight. CONCLUSIONS: Mid-arm circumference and mid-arm/head circumference ratio values were established for the studied population. It was possible to obtain curves for both mid-arm circumference and mid-arm/head circumference ratio in relation to birth weight. However, for mid-arm circumference, it was only possible to obtain curves in relation to gestational age. The use of the regression curves did not seem powerful enough to predict the mid-arm circumference and mid-arm/head circumference ratio in this population of term newborns. There were no gender differences for either of the measurements studied.

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A New Method for Ranking Interval Type-2 Fuzzy Numbers Based on Mellin Transform

International Journal of Uncertainty Fuzziness and Knowledge-Based Systems ◽

10.1142/s0218488520500257 ◽

2020 ◽

Vol 28 (04) ◽

pp. 591-611

Author(s):

Yanbing Gong ◽

Lin Xiang ◽

Shuxin Yang ◽

Hailiang Ma

Keyword(s):

Fuzzy Sets ◽

Degrees Of Freedom ◽

Mellin Transform ◽

Fuzzy Numbers ◽

Mean Value ◽

Distance Measures ◽

Mean Values ◽

Interval Type

Interval type-2 fuzzy sets provide us with additional degrees of freedom to represent the uncertainty and the fuzziness of the real word than traditional type-1 fuzzy sets. Interval type-2 fuzzy numbers ranking has an important role in the decision making analysis. In this paper, the probatilistic mean value and variance of interval type-2 fuzzy numbers are proposed based on the Mellin transform for type-1 fuzzy numbers. The interval type-2 fuzzy number with the higher mean is ranked higher. If the mean values are equal the one with the smaller variance is judged higher rank. On this basis, some new distance measures and possibility degree formula are proposed to comparing interval type-2 fuzzy numbers based on their Mellin mean value and variance. Some benchmarking numerical examples are given, and some interpretation issues are explained.

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