scholarly journals Distribution and Appearance of Ki-67, IL-1α, IL-10, and PGP 9.5 in Reinke’s Oedema-Affected Larynx Tissue Compared with Control Tissue

Life ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1379
Author(s):  
Vita Konopecka ◽  
Mara Pilmane ◽  
Dins Sumerags ◽  
Gunta Sumeraga
Keyword(s):  
Patient Group ◽  
Vocal Fold ◽  
Laryngopharyngeal Reflux ◽  
Interleukin 1 ◽  
Tissue Response ◽  
Surface Epithelium ◽  
Control Group ◽  
Ki 67 ◽  
Pgp 9.5 ◽  
And Control

Smoking, laryngopharyngeal reflux, and vocal fold abuse can promote the development of Reinke’s oedema, leading to vocal fold dysfunction and injury. The aim of the work was to investigate the appearance and distribution of proliferation marker Ki-67 (Ki-67), interleukin 10 (IL-10), interleukin 1 alpha (IL-1α), and protein gene peptide 9.5 (PGP 9.5) in Reinke’s oedema-affected larynx tissue. Methods: A routine histological and immunohistochemical Reinke’s oedema and control group patient analysis was conducted. We used the biotin–streptavidin biochemical method to detect Ki-67, IL-10, IL-1α, and PGP 9.5 The semiquantitative grading method was used to evaluate immunoreactive cells' appearance and local distribution. A Mann–Whitney U test and Spearman’s rank coefficient were performed. Results: A low positive correlation between IL-1α epithelial and subepithelial immunoreactive cells in the patient group was found. Mann–Whitney U tests revealed significant patient and control group immunoreactive marker differences. All examined markers showed a higher number of immunoreactive structures in the patient group. Conclusions: Intensive proliferation of the surface epithelium was observed in patient tissues. The notable increase in IL-10 positive structures indicates the dominant anti-inflammatory tissue response. An increased number of IL-1α structures in the larynx epithelium and subepithelium in the patient group is linked to inflammation, proliferation, and tissue remodelling. The PGP 9.5 expression increase is involved in the morphopathogenesis of Reinke’s oedema.

scholarly journals Distribution and Appearance of Ki-67, IL-1α, IL-10 and PGP 9.5 in Reinke’s Oedema-affected Larynx Tissue Compared With Control Tissue

2020 ◽  
Author(s):  
Vita Konopecka ◽  
Mara Pilmane ◽  
Dins Sumerags ◽  
Gunta Sumeraga
Keyword(s):  
Vocal Fold ◽  
Laryngopharyngeal Reflux ◽  
Vocal Folds ◽  
Tissue Response ◽  
Surface Epithelium ◽  
Control Group ◽  
Ki 67 ◽  
High Positive Correlation ◽  
Pgp 9.5 ◽  
Control Tissue

Abstract Background: Reinke’s oedema (benign diffuse swelling of the vocal folds) induced various morphological changes occurs in the superficial lamina propria also known as the Reinke’s space. Smoking, laryngopharyngeal reflux and vocal fold abuse can promote the development of this condition as well as lead to vocal fold dysfunction and injury. Patients with Reinke’s oedema complain of vocal problems. 3,47/1000 is estimation of the general population prevalence of Reinke’s oedema. Investigation of the distribution and appearance of Ki-67, IL-1α, IL-10 and PGP 9.5 in Reinke’s oedema-affected larynx tissue compared with control tissue was the aim of the work.Methods: Routine histological and immunohistochemical analysis were applied for specimens with Reinke’s oedema and the control group. Biotin-streptavidin biochemical method was used for detection of Ki-67, PGP 9,5, IL-10 and IL-1α. Immunoreactive cells appearance local distribution were evaluated using semiquantitative grading method. Statistical analysis of the data was performed using nonparametric statistical methods (Mann Whitney U test and Spearman’s rank coefficient).Results: A high positive correlation was found between IL-1α and PGP 9.5 epithelial immunoreactive cells and between epithelial and subepithelial IL-10 cells in the control group. Mann-Whitney U tests revealed significant differences in immunoreactive markers between the patients and the control group. With exception of PGP 9.5 positive subepithelial nerves, all other examined markers revelated higher number of immunoreactive positive structures in the patient tissue.Conclusions: Intensive proliferation of the surface epithelium was observed in Reinke’s oedema affected larynx tissue. Increased expression of IL-1 α structures is linked to tissue remodulation, inflammation and remodulation. Notable increase in IL-10 positive structures is indicating to the dominant anti-inflammatory tissue response. PGP 9.5 expression increase is involved in the morphopathogenesis of Reinke’s oedema.Trial registration: This study was approved by the Ethical Committee of Riga Stradins University, issued on 31-10-2019, No 6-2/2/25.

A gender-specific association of interleukin 1 receptor antagonist polymorphism with schizophrenia susceptibility

2013 ◽  
Vol 25 (6) ◽  
pp. 349-355 ◽  
Author(s):  
Mouna Ben Nejma ◽  
Ines Zaabar ◽  
Ferid Zaafrane ◽  
Sihem Thabet ◽  
Anouar Mechri ◽  
...  
Keyword(s):  
Genetic Polymorphism ◽  
Receptor Antagonist ◽  
Patient Group ◽  
Interleukin 1 ◽  
Tunisian Population ◽  
Control Group ◽  
Altered Level ◽  
Interleukin 1 Receptor Antagonist ◽  
Increased Risk ◽  
Intron 2

ObjectiveRecent genetic studies have revealed that the interleukin (IL) 1 gene complex is associated with schizophrenia in the Caucasian population; however, data from the North African population are limited. To further assess the role of interleukin 1 receptor antagonist protein (IL1Ra) in schizophrenia, we examined a functional multiallelic polymorphism localised in intron 2 of this receptor gene associated with an altered level of IL1Ra.MethodsIn the present case–control study, we have analysed the (86 bp)npolymorphism of the interleukin 1 receptor antagonist (IL1RN) gene (RS 1794068) by polymerase chain reaction genotyping in 259 patients with schizophrenia and 178 healthy controls from the Tunisian population.ResultsWe showed that the frequencies of the IL1RN*2/2 genotype and allele 2 were higher in the patient group compared with the control group, and the difference was statistically significant [13.5% vs. 5.6%,p= 10−3, odds ratio (OR) = 3.2% and 32.8% vs. 21.9%,p= 3 × 10−4, OR = 1.76, respectively). When we evaluated the association between this genetic polymorphism and the clinical variables of schizophrenia, we found that the frequencies of the 2/2 genotype and allele 2 were significantly higher in the male patient group (p= 10−4and 10−5, respectively) compared with the male control group, indicating a substantially increased risk for sex-onset schizophrenia with inheritance of the IL1RN2 allele. When the association between the genotypes and outcome was evaluated by multiple logistic regression analysis, the adjusted OR for the IL1RN genotypes remained statistically significant [1.39; 95% confidence interval (CI) = 1.11–1.73;p= 0.003].ConclusionThe intron 2 polymorphism in IL1RN or a genetic polymorphism at proximity seems to be associated specifically with schizophrenia in the Tunisian male population.

scholarly journals Study of IL-33 and IL-1R4 in Iraqi Rheumatoid Arthritis Female Patient's with and without Dyslipidemia Prone to Atherosclerosis

2019 ◽  
Vol 32 (1) ◽  
pp. 47
Author(s):  
Lara Ali Nazar ◽  
Eiman AA.` Abass
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Interleukin 1 ◽  
Control Group ◽  
Control Groups ◽  
Interleukin 33 ◽  
Erythrocyte Sedimentation ◽  
And Control

   This study is planned to find relationship between interleukin-33 (IL-33) with its receptor interleukin-1 receptor 4 (IL-1R4), and assurance IL-33/IL-1R4 proportion as biomarker to atherosclerosisin rheumatoid arthritis (RA) Iraqi females patients with and without dyslipidemia. This study was attempted at Baghdad Teaching Hospital included 60 females patients with RA that were isolated into: 30 patients with dyslipidemia(G2), 30 patients without dyslipidemia(G3) and 30 individual as control group (G1) .Patients were experiencing treatment by methortexiene medication, analyzed by rheumatoid factor(RF) and erythrocyte sedimentation rate( ESR) tests. All patients and control groups age ranged from (30-55) years. The results show an increase in ESR, RF, IL-33, and IL-1R4 levels. In addition to decrease in IL-33/IL-1R4 ratio in the two patients groups when contrasted and control group. The momentum examine inferred that the level of ESR, and  IL-33 in RA Iraqi females  patients with dyslipidemia were higher than that in RA Iraqi females patients without dyslipidemia, while the level of IL-33/IL-1R4 ratio in RA Iraqi females patients with dyslipidemia was lower than that in RA Iraqi females patients without dyslipidemia patients; in this manner the IL-33/IL-1R4 ratio may be used as a biomarker in diagnostic early porn to atherosclerosis in RA females patients with dyslipidemia

The G allele in IL-10-1082 G/A may have a role in lowering the susceptibility to panic disorder in female patients

2016 ◽  
Vol 28 (6) ◽  
pp. 357-361 ◽  
Author(s):  
Han-Joon Kim ◽  
Yong-Ku Kim
Keyword(s):  
Panic Disorder ◽  
Patient Group ◽  
Control Group ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Female Patients ◽  
Immune System Activation ◽  
Tnf Α ◽  
System Activation ◽  
And Control

BackgroundImmune system activation is involved in the pathophysiology of panic disorder (PD). We investigated INF-γ+874 A/T, TNF-α-308 G/A, and IL-10-1082 G/A single nucleotide polymorphisms (SNPs) to determine their association with PD.MethodThis study enroled 135 PD patients and 135 healthy controls. INF-γ+874 A/T (rs2430561), TNF-α-308 G/A (rs1800629), and IL-10-1082 G/A (rs1800896) were genotyped.ResultsThere were no differences in genotypes or allele frequencies between the patient and control groups, regardless of accompanying agoraphobia. However, for female patients, the G allele frequency in IL-10 SNP was higher in the control group than in the patient group. Additionally, the female control group had a higher frequency of the A/G and G/G genotype in the IL-10 SNP than the female patient group.ConclusionWe suggest that the G allele in IL-10-1082 G/A might have a role in reducing the manifestations of PD in female patients. Further studies are needed to extend and confirm our findings.

scholarly journals Expression of matrix metalloproteinases (MMP-2 and MMP-9) in vocal fold polyps

Medicina ◽  
2008 ◽  
Vol 44 (4) ◽  
pp. 322 ◽  
Author(s):  
Vaiva Lesauskaitė ◽  
Virgilijus Uloza ◽  
Vykintas Liutkevičius ◽  
Dalia Pangonytė
Keyword(s):  
Matrix Metalloproteinases ◽  
Normal Tissue ◽  
Vocal Fold ◽  
Vocal Folds ◽  
Control Group ◽  
Significant Difference ◽  
Malignant Lesions ◽  
Benign Laryngeal Lesions ◽  
And Control ◽  
Epithelium Cells

Objective. Vocal fold polyps are the most common benign laryngeal lesions. Matrix metalloproteinases (MMP) play an important role in the physiological and pathological remodeling of tissues. The most important subgroup of MMP family consists of gelatinases A and B (MMP-2 and MMP-9). The objective of this study was investigation of the expression of MMP-2 and MMP-9 in vocal fold polyps and normal tissue of vocal folds. Material and methods. The immunohistochemical expression of MMP-2 and MMP-9 was investigated in specimens taken by endolaryngeal microsurgery from vocal fold polyps (n=30) and normal tissue of vocal fold (n=13, control group). Expression of MMP-2 and MMP-9, both in epithelium and stroma cells, was graded on a semiquantitative scale, ranging from 0 (no expression) to 6 points (high expression). Results. A statistically significant increase was observed in the expression of MMP-2 in stroma cells (P=0.0176) of vocal fold polyps compared to control vocal fold group, whereas no significant difference in the expression of MMP-2 was found in epithelium cells (P=0.1487). Comparison of expression of MMP-2 and MMP-9 in epithelium cells revealed a statistically significant increase in MMP-9 expression (P<0.01) in both groups. However, there was no statistically significant difference in the expression of MMP-9 between groups of vocal fold polyps and control vocal folds. Conclusion. Expression of MMP-2 in stroma was significantly higher in polyps than in normal tissue of vocal folds. Our data draw attention to the role of MMP-2 in the development of vocal fold polyps and necessity of further investigations to define its function in morphogenesis of laryngeal benign, premalignant, and malignant lesions.

scholarly journals Distinguishing and Biochemical Phenotype Analysis of Epilepsy Patients Using a Novel Serum Profiling Platform

2020 ◽  
Vol 10 (8) ◽  
pp. 504
Author(s):  
Jay S. Hanas ◽  
James R. S. Hocker ◽  
Christian Vannarath ◽  
Betcy Evangeline ◽  
Vasudevan Prabhakaran ◽  
...  
Keyword(s):  
Patient Group ◽  
Structural Changes ◽  
Control Group ◽  
Ionization Mass ◽  
Epilepsy Patient ◽  
Control Groups ◽  
Serum Profiling ◽  
Phenotype Analysis ◽  
Symptomatic Epilepsy ◽  
And Control

Diagnosis of non-symptomatic epilepsy includes a history of two or more seizures and brain imaging to rule out structural changes like trauma, tumor, infection. Such analysis can be problematic. It is important to develop capabilities to help identify non-symptomatic epilepsy in order to better monitor and understand the condition. This understanding could lead to improved diagnostics and therapeutics. Serum mass peak profiling was performed using electrospray ionization mass spectrometry (ESI-MS). A comparison of sera mass peaks between epilepsy and control groups was performed via leave one [serum sample] out cross-validation (LOOCV). MS/MS peptide analysis was performed on serum mass peaks to compare epilepsy patient and control groups. LOOCV identified significant differences between the epilepsy patient group and control group (p = 10−22). This value became non-significant (p = 0.10) when the samples were randomly allocated between the groups and reanalyzed by LOOCV. LOOCV was thus able to distinguish a non-symptomatic epilepsy patient group from a control group based on physiological differences and underlying phenotype. MS/MS was able to identify potential peptide/protein changes involved in this epilepsy versus control comparison, with 70% of the top 100 proteins indicating overall neurologic function. Specifically, peptide/protein sera changes suggested neuro-inflammatory, seizure, ion-channel, synapse, and autoimmune pathways changing between epilepsy patients and controls.

scholarly journals Sirolimus influence on hepatectomy-induced liver regeneration in rats

2014 ◽  
Vol 41 (3) ◽  
pp. 203-207 ◽  
Author(s):  
Edimar Leandro Toderke ◽  
Giorgio Alfredo Pedroso Baretta ◽  
Ozimo Pereira Gama Filho ◽  
Jorge Eduardo Fouto Matias
Keyword(s):  
Liver Regeneration ◽  
Negative Influence ◽  
Control Group ◽  
Cell Multiplication ◽  
Study Group ◽  
Adult Rats ◽  
Ki 67 ◽  
Immunohistochemical Markers ◽  
Significant Difference ◽  
And Control

OBJECTIVE: To evaluate the influence of sirolimus on liver regeneration triggered by resection of 70% of the liver of adult rats. METHODS: we used 40 Wistar rats randomly divided into two groups (study and control), each group was divided into two equal subgroups according to the day of death (24 hours and seven days). Sirolimus was administered at a dose of 1mg/kg in the study group and the control group was given 1 ml of saline. The solutions were administered daily since three days before hepatectomy till the rats death to removal of the regenerated liver, conducted in 24 hours or 7 days after hepatectomy. Liver regeneration was measured by the KWON formula, by thenumber of mitotic figures (hematoxylin-eosin staining) and by the immunohistochemical markers PCNA and Ki-67. RESULTS: there was a statistically significant difference between the 24h and the 7d groups. When comparing the study and control groups in the same period, there was a statistically significant variation only for Ki-67, in which there were increased numbers of hepatocytes in cell multiplication in the 7d study group compared with the 7d control group (p = 0.04). CONCLUSION: there was no negative influence of sirolimus in liver regeneration and there was a positive partial effect at immunohistochemistry with Ki-67.

Personality and Voice Disorders

2000 ◽  
Vol 43 (3) ◽  
pp. 749-768 ◽  
Author(s):  
Nelson Roy ◽  
Diane M. Bless ◽  
Dennis Heisey
Keyword(s):  
Vocal Fold ◽  
Voice Disorders ◽  
Personality Factors ◽  
Control Group ◽  
Personality Variables ◽  
Personality Differences ◽  
Functional Dysphonia ◽  
Vocal Nodules ◽  
Personality Features ◽  
And Control

To determine whether personality factors play causal, concomitant, or consequential roles in common voice disorders, a vocally normal control group and four groups with voice disorders—functional dysphonia (FD), vocal nodules (VN), spasmodic dysphonia (SD), and unilateral vocal fold paralysis (UVFP)—were compared on measures of personality and psychological adjustment. Superfactor group comparisons revealed that the majority of FD and VN subjects were classified as introverts and extraverts, respectively. Comparisons involving the SD, UVFP, and control subjects did not identify consistent personality differences. The disability hypothesis, which suggests that personality features and emotional maladjustment are solely a negative consequence of vocal disability, was not supported. Personality variables and their behavioral consequences may therefore contribute to FD and VN. Results are presented within the context of a dispositional theory offered by Roy and Bless (2000a).

Evaluation of the quality-of-life (QOL) and socio-demographic characteristics of patients with leukemia and lymphoma: Comparison with sibling and control group.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e22524-e22524
Author(s):  
Nurdan Tacyildiz ◽  
Tugba Karakose ◽  
Emel Cabi Unal ◽  
Handan Dincaslan ◽  
Gulsah Tanyildiz ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Patient Group ◽  
Active Treatment ◽  
Healthy Control Group ◽  
Control Group ◽  
Healthy Children ◽  
Healthy Control ◽  
And Control ◽  
Sibling Group

e22524 Background: Pediatric cancer patients have increased survival rates with intensive and effective treatment methods which causing some chronic health problems and lower quality of life in long term follow up. In our study, patients in their active treatment period compared to patients who have completed their treatment in recent 5 years and more than 5 years groups, besides compared with their own siblings and healthy control group,evaluated in terms of quality of life ( QOL). Methods: This study has been daone in the Department of Pediatric Hematology-Oncology, Ankara University School of Medicine. A total of 191 children were included in the study, including 36 sibling, 76 children with leukemia (n:31) and lymphoma (n:45) besides 79 healthy children. The Turkish translation of the PedsQL questionnaire was used to evaluate the patients' quality of life. IBM SPSS-25 package program was used for statistical analysis. Results: When the sociodemographic characteristics of the patient, sibling and control group were examined; we determined that the patients, siblings and control groups were similar in age, age and gender. However, we found that the education level for the patient group was statisticaly signicantly lower then the siblings and the control group. In addition, physical and mental functionning and friendship capasity scores were lower in patient group than the other two groups. According to timing of the treatment; patients and parents quality of life scores were significantly lower than the patients that finished their treatment in recent 5 years or more than 5 years. QOL scores were higest in survivors and parents group that were in more than 5 years after completed the treatment. The risk of lower QOL in patients under treatment was 7.48 times higher than the children who had ≥5 years of treatment (OR = 7,48; p < 0,05), and 2.64 times higher than those who were treated < 5 years (OR = 2,64; p > 0,05). In this study, there were no independent variables that had an impact on QOL of the sibling group. Conclusions: The QOLof patients diagnosed with leukemia and lymphoma is significantly lower than siblings and healthy group. No statistically significant difference was found between the sibling group and the healthy control group. After the end of active treatment, the QOL of the patients was reflecting important increase by the time .

Self-Descriptions of Patients with Coronary Disease

1968 ◽  
Vol 26 (3_suppl) ◽  
pp. 1099-1107 ◽  
Author(s):  
Helen P. Klein ◽  
Oscar A. Parsons
Keyword(s):  
Coronary Disease ◽  
Patient Group ◽  
Control Group ◽  
Coronary Patients ◽  
Mood Scale ◽  
Almost All ◽  
And Control ◽  
Comparable Period ◽  
Over Time

Current self-descriptions of coronary patients and control Ss were obtained on the Clyde Mood Scale. Comparison was made with retrospective self-descriptions, i.e., just prior to the coronary attack for the patients and a comparable period of time for the control group. In addition, spouses' descriptions of Ss for the same periods were obtained. In comparison to controls, the coronary Ss were more “depressed” and “anergic” for both periods. Spouses described patients as more “anergic” following the coronary. Significant correlations in the patient group between the two periods were found on positive subscales but not on “depressed,” “anergic” or “jittery.” Correlations in the control group between the two periods were significant for almost all subscales. It was concluded that the coronary attack has an impact on the patient which does not dissipate over time and that patients react to this impact differentially depending on their defensive systems.

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