scholarly journals Macroporous Calcium Phosphate/Chitosan Composites Prepared via Unidirectional Ice Segregation and Subsequent Freeze-Drying

Materials ◽  
2017 ◽  
Vol 10 (5) ◽  
pp. 516 ◽  
Author(s):  
Inmaculada Aranaz ◽  
Enrique Martínez-Campos ◽  
Carolina Moreno-Vicente ◽  
Ana Civantos ◽  
Sara García-Arguelles ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Freeze Drying ◽  
Ice Segregation

scholarly journals Carbon nanotube reinforced polyvinyl alcohol/biphasic calcium phosphate scaffold for bone tissue engineering

RSC Advances ◽  
2019 ◽  
Vol 9 (67) ◽  
pp. 38998-39010 ◽  
Author(s):  
Weiwei Lan ◽  
Xiumei Zhang ◽  
Mengjie Xu ◽  
Liqin Zhao ◽  
Di Huang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Calcium Phosphate ◽  
Polyvinyl Alcohol ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Carbon ◽  
Biphasic Calcium Phosphate ◽  
Freeze Drying ◽  
Drying Method

In this paper, a well-developed porous carbon nanotube (CNT) reinforced polyvinyl alcohol/biphasic calcium phosphate (PVA/BCP) scaffold was fabricated by a freeze-thawing and freeze-drying method.

Thermokinetic Investigation of the Drying Conditions on Amorphous Calcium Phosphate

2017 ◽  
Vol 758 ◽  
pp. 204-209
Author(s):  
Agnese Brangule ◽  
Līga Avotiņa ◽  
Artūrs Zariņš ◽  
Mihails Haļitovs ◽  
Kārlis Agris Gross ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Crystalline Structure ◽  
Amorphous Calcium Phosphate ◽  
Freeze Drying ◽  
Calcium Phosphates ◽  
Drying Condition ◽  
Drying Conditions

The present work investigated dried calcium phosphate powders which still retain an amorphous or poorly crystalline structure under a variety of conditions. In previous studies, freeze-drying was found to be the optimum drying condition. However, several publications, as well as our previous studies, have shown that calcium phosphate amorphous, or a poorly crystalline structure, can retain their structure even if the samples are dried immediately after synthesis up to 200°C. In our study, we used the thermokinetic studies FTIR and XRD and showed that the samples are amorphous, or poorly crystalline, but were unable to answer the questions: Is there a difference between the differently dried amorphous calcium phosphates? What are the optimum drying conditions under which the amorphous calcium phosphate (ACP) structure loses the physically bounded water, but still retains the chemically bounded water?

Preparation of various calcium-phosphate powders by ultrasonic spray freeze-drying technique

2000 ◽  
Vol 35 (4) ◽  
pp. 575-585 ◽  
Author(s):  
Kiyoshi Itatani ◽  
Kengo Iwafune ◽  
F.Scott Howell ◽  
Mamoru Aizawa
Keyword(s):  
Calcium Phosphate ◽  
Freeze Drying ◽  
Ultrasonic Spray ◽  
Spray Freeze Drying

scholarly journals MICROSTRUCTURES OF SPHERICAL CALCIUM-PHOSPHATE AGGLOMERATES PREPARED BY SPRAY-PYROLYSIS AND FREEZE-DRYING TECHNIQUES

2006 ◽  
Vol 20 ◽  
pp. 47-60 ◽  
Author(s):  
Kiyoshi Itatani ◽  
Tomohiro Umeda ◽  
Yoshiro Musha ◽  
Ian J. Davies
Keyword(s):  
Calcium Phosphate ◽  
Spray Pyrolysis ◽  
Freeze Drying ◽  
Drying Techniques

scholarly journals Preparation of Scaffolds of Amorphous Calcium Phosphate and Bacterial Cellulose for Use in Tissue Regeneration by Freeze-Drying Process

2020 ◽  
Vol 11 (1) ◽  
pp. 7357-7367
Keyword(s):  
Calcium Phosphate ◽  
Bacterial Cellulose ◽  
Tissue Regeneration ◽  
Amorphous Calcium Phosphate ◽  
Freeze Drying ◽  
Calcium Phosphates ◽  
Synthetic Polymers ◽  
Drying Process ◽  
Cytotoxicity Studies ◽  
The One

The elaboration of scaffolds for use in tissue regeneration processes plays an important role in the area of biomaterials. Natural and synthetic polymers, together with calcium phosphates, form suitable compounds for these studies because their combinations favor the union of the properties of both materials, such as their biocompatibility, biofunctionality, shape, porosity, and mechanical properties. The objective of this work was to develop a scaffold of amorphous calcium phosphate and bacterial cellulose, applying a freeze-drying process. The results demonstrated the feasibility of scaffolds elaboration applying the freeze-drying methodology. The formulation that presented the best results was the one that contained amorphous calcium phosphate (50%), bacterial cellulose gel (20%), and sodium alginate (30%). Cytotoxicity studies showed that the studied formulation did not present cytotoxicity, promoting cell viability.

Ca-Histochemistry in slime mold plasmodia

1978 ◽  
Vol 36 (2) ◽  
pp. 130-131
Author(s):  
Ulrich Dierkes
Keyword(s):  
Physarum Polycephalum ◽  
Carbon Coating ◽  
Freeze Drying ◽  
Freeze Fracture ◽  
Freeze Substitution ◽  
Uranyl Acetate ◽  
Slime Mold ◽  
Staining Procedure ◽  
Protoplasmic Streaming ◽  
Intracellular Ca

Calcium is supposed to play an important role in the control of protoplasmic streaming in slime mold plasmodia. The motive force for protoplasmic streaming is generated by the interaction of actin and myosin. This contraction is supposed to be controlled by intracellular Ca-fluxes similar to the triggering system in skeleton muscle. The histochemical localisation of calcium however is problematic because of the possible diffusion artifacts especially in aquous media.To evaluate this problem calcium localisation was studied in small pieces of shock frozen (liquid propane at -189°C) plasmodial strands of Physarum polycephalum, which were further processed with 3 different methods: 1) freeze substitution in ethanol at -75°C, staining in 100% ethanol with 1% uranyl acetate, and embedding in styrene-methacrylate. For comparison the staining procedure was omitted in some preparations. 2)Freeze drying at about -95°C, followed by immersion with 100% ethanol containing 1% uranyl acetate, and embedding. 3) Freeze fracture, carbon coating and SEM investigation at temperatures below -100° C.

The Critical Point Drying Method Applied to Scanning Electron Microscopy of L-929 Cells

1970 ◽  
Vol 28 ◽  
pp. 278-279
Author(s):  
Charles TurnbiLL ◽  
Delbert E. Philpott
Keyword(s):  
Electron Microscopy ◽  
Carbon Dioxide ◽  
Surface Tension ◽  
Critical Point ◽  
Freeze Drying ◽  
Attractive Alternative ◽  
Crystal Formation ◽  
Critical Point Drying ◽  
Time Required ◽  
Scanning Electron

The advent of the scanning electron microscope (SCEM) has renewed interest in preparing specimens by avoiding the forces of surface tension. The present method of freeze drying by Boyde and Barger (1969) and Small and Marszalek (1969) does prevent surface tension but ice crystal formation and time required for pumping out the specimen to dryness has discouraged us. We believe an attractive alternative to freeze drying is the critical point method originated by Anderson (1951; for electron microscopy. He avoided surface tension effects during drying by first exchanging the specimen water with alcohol, amy L acetate and then with carbon dioxide. He then selected a specific temperature (36.5°C) and pressure (72 Atm.) at which carbon dioxide would pass from the liquid to the gaseous phase without the effect of surface tension This combination of temperature and, pressure is known as the "critical point" of the Liquid.

Preservation of Plant Cell Surfaces For Scanning Electron Microscopy

1973 ◽  
Vol 31 ◽  
pp. 472-473
Author(s):  
Linda M. Sicko ◽  
Thomas E. Jensen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Critical Point ◽  
Filter Paper ◽  
Freeze Drying ◽  
Cell Surfaces ◽  
Air Drying ◽  
Popular Method ◽  
Critical Point Drying ◽  
Scanning Electron

The use of critical point drying is rapidly becoming a popular method of preparing biological samples for scanning electron microscopy. The procedure is rapid, and produces consistent results with a variety of samples. The preservation of surface details is much greater than that of air drying, and the procedure is less complicated than that of freeze drying. This paper will present results comparing conventional air-drying of plant specimens to critical point drying, both of fixed and unfixed material. The preservation of delicate structures which are easily damaged in processing and the use of filter paper as a vehicle for drying will be discussed.

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