Biological Performance of Electrospun Polymer Fibres

The evaluation of biological responses to polymeric scaffolds are important, given that the ideal scaffold should be biocompatible, biodegradable, promote cell adhesion and aid cell proliferation. The primary goal of this research was to measure the biological responses of cells against various polymeric and collagen electrospun scaffolds (polycaprolactone (PCL) and polylactic acid (PLA) polymers: PCL–drug, PCL–collagen–drug, PLA–drug and PLA–collagen–drug); cell proliferation was measured with a cell adhesion assay and cell viability using 5-bromo-2′-deoxyuridine (BrdU) and resazurin assays. The results demonstrated that there is a distinct lack of growth of cells against any irgasan (IRG) loaded scaffolds and far greater adhesion of cells against levofloxacin (LEVO) loaded scaffolds. Fourteen-day studies revealed a significant increase in cell growth after a 7-day period. The addition of collagen in the formulations did not promote greater cell adhesion. Cell viability studies revealed the levels of IRG used in scaffolds were toxic to cells, with the concentration used 475 times higher than the EC50 value for IRG. It was concluded that the negatively charged carboxylic acid group found in LEVO is attracting positively charged fibronectin, which in turn is attracting the cell to adhere to the adsorbed proteins on the surface of the scaffold. Overall, the biological studies examined in this paper are valuable as preliminary data for potential further studies into more complex aspects of cell behaviour with polymeric scaffolds.

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Screening for novel cell adhesive regions in bovine Achilles tendon collagen peptides

Biochemistry and Cell Biology ◽

10.1139/bcb-2013-0026 ◽

2014 ◽

Vol 92 (1) ◽

pp. 9-22 ◽

Cited By ~ 6

Author(s):

Pradipta Banerjee ◽

Alka Mehta ◽

C. Shanthi

Keyword(s):

Cell Adhesion ◽

Docking Studies ◽

Exchange Chromatography ◽

Adhesion Assay ◽

Structural Protein ◽

Cell Adherence ◽

Collagen Peptides ◽

Adhesion Ability ◽

A Cell ◽

Hydrophilic Residues

Collagen, a major structural protein of the ECM, is known for its high cell adherence capacity. This study was conducted to identify regions in collagen that harbour such bioactivity. Collagen from tendon was hydrolysed and the peptides fractionated using ion-exchange chromatography (IEC). Isolated peptide fractions were coated onto disposable dishes and screened for cell adherence and proliferative abilities. Active IEC fractions were further purified by chromatography, and two peptides, C2 and E1 with cell adhesion ability, were isolated. A cell adhesion assay done with different amounts of C2 coated onto disposable dishes revealed the maximum adhesion to be 94.6%, compared with 80% for collagen coated dishes and an optimum peptide coating density of 0.507 nmoles per cm2 area of the dish. Growth of cells on C2, collagen, and E1 revealed a similar pattern and a reduction in the doubling time compared with cells grown on uncoated dishes. C2 had a mass of 2.046 kDa with 22 residues, and sequence analysis revealed a higher percentage occurrence of hydrophilic residues compared with other regions in collagen. Docking studies revealed GDDGEA in C2 as the probable site of interaction with integrins α2β1 and α1β1, and stability studies proved C2 to be mostly protease-resistant.

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Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay

Journal of Visualized Experiments ◽

10.3791/187 ◽

2007 ◽

Cited By ~ 1

Author(s):

Souichi Ogata ◽

Ken W.Y. Cho

Keyword(s):

Cell Adhesion ◽

Xenopus Laevis ◽

Adhesion Assay ◽

Animal Pole ◽

Cell Adhesion Assay ◽

A Cell ◽

Xenopus Laevis Embryos

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Nanofibrillar cellulose wound dressing supports the growth and characteristics of human mesenchymal stem/stromal cells without cell adhesion coatings

Stem Cell Research & Therapy ◽

10.1186/s13287-019-1394-7 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 5

Author(s):

Jasmi Kiiskinen ◽

Arto Merivaara ◽

Tiina Hakkarainen ◽

Minna Kääriäinen ◽

Susanna Miettinen ◽

...

Keyword(s):

Wound Healing ◽

Cell Adhesion ◽

Cell Viability ◽

Cell Survival ◽

Cell Transplantation ◽

Stromal Cells ◽

Wound Dressing ◽

Nanofibrillar Cellulose ◽

Cell Scaffold ◽

A Cell

Abstract Background In the field of regenerative medicine, delivery of human adipose-derived mesenchymal stem/stromal cells (hASCs) has shown great promise to promote wound healing. However, a hostile environment of the injured tissue has shown considerably to limit the survival rate of the transplanted cells, and thus, to improve the cell survival and retention towards successful cell transplantation, an optimal cell scaffold is required. The objective of this study was to evaluate the potential use of wood-derived nanofibrillar cellulose (NFC) wound dressing as a cell scaffold material for hASCs in order to develop a cell transplantation method free from animal-derived components for wound treatment. Methods Patient-derived hASCs were cultured on NFC wound dressing without cell adhesion coatings. Cell characteristics, including cell viability, morphology, cytoskeletal structure, proliferation potency, and mesenchymal cell and differentiation marker expression, were analyzed using cell viability assays, electron microscopy, immunocytochemistry, and quantitative or reverse transcriptase PCR. Student’s t test and one-way ANOVA followed by a Tukey honestly significant difference post hoc test were used to determine statistical significance. Results hASCs were able to adhere to NFC dressing and maintained high cell survival without cell adhesion coatings with a cell density-dependent manner for the studied period of 2 weeks. In addition, NFC dressing did not induce any remarkable cytotoxicity towards hASCs or alter the morphology, proliferation potency, filamentous actin structure, the expression of mesenchymal vimentin and extracellular matrix (ECM) proteins collagen I and fibronectin, or the undifferentiated state of hASCs. Conclusions As a result, NFC wound dressing offers a functional cell culture platform for hASCs to be used further for in vivo wound healing studies in the future.

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93. Mechanisms Implicated In Cell Proliferation and Cell Survival Induced By Recombinant Losac, A Cell Adhesion Molecule From Lonomia oblique

Toxicon ◽

10.1016/j.toxicon.2012.04.094 ◽

2012 ◽

Vol 60 (2) ◽

pp. 142-143

Author(s):

Miryam P. Alvarez-Flores ◽

Cesar M. Remuzgo ◽

Yara Cury ◽

Rosemary V. Bosch ◽

Beatriz B. Vaz-De-Lima ◽

...

Keyword(s):

Cell Proliferation ◽

Cell Adhesion ◽

Cell Survival ◽

Adhesion Molecule ◽

Cell Adhesion Molecule ◽

A Cell

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Pentamidine Is a Specific, Non-Peptide, GPIIb/llla Antagonist

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650305 ◽

1996 ◽

Vol 75 (03) ◽

pp. 503-509 ◽

Cited By ~ 12

Author(s):

Dermot Cox ◽

Toshiaki Aoki ◽

Jiro Seki ◽

Yukio Motoyama ◽

Keizo Yoshida

Keyword(s):

Monoclonal Antibody ◽

Cell Adhesion ◽

Endothelial Cell ◽

Intracellular Ph ◽

Platelet Adhesion ◽

Adhesion Assay ◽

Agonist Peptide ◽

A Cell ◽

Granule Release ◽

Endothelial Cell Adhesion

SummaryPentamidine was previously shown to act on glycoprotein (GP) Ilb/IIIa (Cox et al., Thromb Haemost 1992; 68: 731). In this paper we study the effect of pentamidine on other RGD-dependent receptors. In a cell adhesion assay, pentamidine was 500 times more potent than RGDS at inhibiting platelet adhesion to fibrinogen. While RGDS inhibited platelet adhesion to fibronectin, endothelial cell adhesion to vitronectin or fibronectin, 293 cell adhesion to vitronectin, IMR 32 cell adhesion to fibronectin and C32 cell adhesion to vitronectin; pentamidine failed to inhibit these interactions at doses as high as 1 mM. Resting platelets fixed in the presence of 1 mM RGDS had increased binding of fibrinogen, i.e., RGDS activated GPIMIIa, while pentamidine at 100 ΜM had no effect. Similarly, RGDS induced the binding of an anti-LIBS monoclonal antibody, while pentamidine had no effect. Pentamidine partially, but significantly, inhibited lysosome and a-granule release induced by the thrombin agonist peptide, while RGDS had no effect. Neither pentamidine nor RGDS affected ADP-induced Ca2+ influx. Pentamidine had no effect on ADP-induced intracellular pH changes while RGDS prevented the pH from returning to normal. Thus, pentamidine is a non-peptide GPIIb/IIIa antagonist that is non-activating and is specific for GPIIb/IIIa.

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Biochemical and molecular biological studies on a cell adhesion molecule involved in memory consolidation

Neurochemistry International ◽

10.1016/0197-0186(92)91736-g ◽

1992 ◽

Vol 21 ◽

pp. S6

Author(s):

R. Schmidt

Keyword(s):

Cell Adhesion ◽

Adhesion Molecule ◽

Memory Consolidation ◽

Cell Adhesion Molecule ◽

Biological Studies ◽

A Cell

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Efficient synthesis of novel RGD based peptides and the conjugation of the pyrazine moiety to their N-terminus

New Journal of Chemistry ◽

10.1039/c8nj04874f ◽

2019 ◽

Vol 43 (6) ◽

pp. 2702-2709

Author(s):

Fatima Hamdan ◽

Zahra Bigdeli ◽

Saeed Balalaie ◽

Norbert Sewald ◽

Carmela Michalek

Keyword(s):

Cell Adhesion ◽

Adhesion Assay ◽

Efficient Synthesis ◽

Cell Adhesion Assay ◽

N Terminus ◽

A Cell

Novel RGD based peptides (RGDFAKLF and RGDNGRG) were designed and synthesized and were later coupled to the pyrazine moiety at the N-terminus. The IC50 values from the in vitro study of the target peptides using a cell adhesion assay indicated the essential impact of the existence of the pyrazine moiety. Meanwhile, peptide 4 exhibited the best IC50 value.

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Synthesis and SAR of a Library of Cell-Permeable Biotin-R8ERY* Peptidomimetics Inhibiting α4β7 Integrin Mediated Adhesion of TK-1 Cells to MAdCAM-1-Fc

Australian Journal of Chemistry ◽

10.1071/ch12227 ◽

2012 ◽

Vol 65 (9) ◽

pp. 1349 ◽

Cited By ~ 1

Author(s):

Stefanie Papst ◽

Anaïs F. M. Noisier ◽

Margaret A. Brimble ◽

Yi Yang ◽

Yih-Chih Chan ◽

...

Keyword(s):

Cell Adhesion ◽

Disease Type ◽

Adhesion Assay ◽

Tyrosine Residue ◽

Regulatory Domain ◽

Lead Compound ◽

Disease States ◽

A Cell ◽

Inflammatory Bowel

The α4β7 integrin is a well‐known target for the development of drugs against various inflammatory disease states including inflammatory bowel disease, type 1 diabetes, and multiple sclerosis. The β7 subunit contains the cell adhesion regulatory domain (CARD) motif YDRREY within its cytoplasmic domain, which is an effective peptide agent for inhibiting T-cell adhesion. The synthesis of a library of cell-permeable β7 integrin inhibitors based on the shortened biotin-R8ERY (R8 = (l-arginine)8) motif is reported, wherein the tyrosine residue has been modified. The synthesised peptidomimetics were evaluated in a cell adhesion assay and shown to inhibit Mn2+-activated adhesion of mouse TK-1 T-cells to mouse MAdCAM-1. Several analogues exhibited improved activity to that of the tyrosine-containing lead compound 1 (biotin-R8ERY). Specifically, analogues 4, 10, and 22 bearing a 4-chloro, a 4-nitro, and a 3,3-diphenyl substituent showed an increase in activity of approximately two-fold compared with that of the initial lead compound. The six most active compounds of the tested series had IC50’s between 25 and 50 μM.

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Antioxidant-Rich Extracts of Terminalia ferdinandiana Interfere with Estimation of Cell Viability

Antioxidants ◽

10.3390/antiox8060191 ◽

2019 ◽

Vol 8 (6) ◽

pp. 191 ◽

Cited By ~ 3

Author(s):

Saleha Akter ◽

Rama Addepalli ◽

Michael E. Netzel ◽

Ujang Tinggi ◽

Mary T. Fletcher ◽

...

Keyword(s):

Cell Proliferation ◽

Cell Viability ◽

Plant Extracts ◽

Direct Reduction ◽

Proliferation Assay ◽

Free System ◽

Cell Proliferation Assay ◽

A Cell ◽

The Impact ◽

Terminalia Ferdinandiana

The impact of plant extracts and phytochemicals on in vitro cell viability is usually assessed by employing cell viability assays dependent upon the activity of dehydrogenase enzymes. The CellTiter 96® AQueous One Solution Cell Proliferation Assay (CellTiter) was used to measure cell viability in response to antioxidant-rich extracts of Terminalia ferdinandiana fruits. Conflicting results were obtained from this assay whereby higher concentrations of extracts significantly increased cell viability compared to lower concentrations. Intrinsic reductive potential was observed in a cell-free system when extracts were added directly to the CellTiter assay reagent. To confirm this effect in a similar cell proliferation assay, we employed the CellTiter-Blue® Cell Viability Assay and again observed increased viability with increased concentrations of the extracts and direct reduction of the assay reagent by the extracts in cell-free systems. In the search for a cell proliferation assay that would not be directly affected by the plant extracts, we identified the CyQUANT® NF Cell Proliferation Assay that is based on the estimation of DNA content in viable cells. Cell viability decreased with increasing concentrations of the extracts. Accordingly, the results of the present study indicated that cell viability assays reliant upon dehydrogenase activity may lead to false positive results when testing antioxidant-rich plant extracts with intrinsic reductive potential, and alternative cell viability assays should be used to measure the cell viability.

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