Evaluation of Light-Emitting Diodes’ Effects on the Expression Level of P53 and EGFR in the Gingival Tissues of Albino Rats

Background and objectives: The light-curing unit is considered an essential piece of equipment in every dental office. This study was conducted to evaluate the effect of Light-Emitting Diodes (LEDs) by the light cure (LC) device on gingival tissues of albino rats histologically and by regarding the expression of P53 and epidermal growth factor receptor (EGFR). Materials and methods: Gingival tissues of the rats were exposed to LEDs for 30 s with an interval of 30 s for periods of 2 and 5 min and were examined after two and four weeks of light exposure. After the set time, histological sections were studied and the P53 and EGFR expressions were evaluated immunohistochemically and by molecular methods. Results: Mild hyperplasia and mild inflammatory response were detected in higher rates after two weeks of exposure when compared to 4 weeks postexposure. Whereas fibrosis was found at a higher rate after four weeks than that found after two weeks postexposure, parakeratosis was seen only in the group that was exposed for 5 min to LC and when biopsies were taken after 2 weeks. We found that the immunohistochemical expression of P53 was not changed. Similarly, the alteration of EGFR expression was statistically nonsignificant (p > 0.05) when compared to the control group. The data obtained from the qRT-PCR reaction was analyzed using the comparative CT (2-ΔΔCT) method. Statistically, there was no significant difference in the expression of EGER and P53 gene transcripts. Conclusions: LED causes no serious alteration in P53 and EGFR expression, and only trivial histopathological changes occurred, most of which recovered after a 4-week interval.

Download Full-text

The Antioxidant Effect of Curcumin and Rutin on Oxidative Stress Biomarkers in Experimentally Induced Periodontitis in Hyperglycemic Wistar Rats

Molecules ◽

10.3390/molecules26051332 ◽

2021 ◽

Vol 26 (5) ◽

pp. 1332

Author(s):

Gilda M. Iova ◽

Horia Calniceanu ◽

Adelina Popa ◽

Camelia A. Szuhanek ◽

Olivia Marcu ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Diabetic Rats ◽

Gingival Tissue ◽

Control Group ◽

Albino Rats ◽

Oxidative Stress Biomarkers ◽

Significant Difference ◽

The Impact ◽

Experimentally Induced

Background: There is a growing interest in the correlation between antioxidants and periodontal disease. In this study, we aimed to investigate the effect of oxidative stress and the impact of two antioxidants, curcumin and rutin, respectively, in the etiopathology of experimentally induced periodontitis in diabetic rats. Methods: Fifty Wistar albino rats were randomly divided into five groups and were induced with diabetes mellitus and periodontitis: (1) (CONTROL)—control group, (2) (DPP)—experimentally induced diabetes mellitus and periodontitis, (3) (DPC)—experimentally induced diabetes mellitus and periodontitis treated with curcumin (C), (4) (DPR)—experimentally induced diabetes mellitus and periodontitis treated with rutin (R) and (5) (DPCR)—experimentally induced diabetes mellitus and periodontitis treated with C and R. We evaluated malondialdehyde (MDA) as a biomarker of oxidative stress and reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG and catalase (CAT) as biomarkers of the antioxidant capacity in blood harvested from the animals we tested. The MDA levels and CAT activities were also evaluated in the gingival tissue. Results: The control group effect was statistically significantly different from any other groups, regardless of whether or not the treatment was applied. There was also a significant difference between the untreated group and the three treatment groups for variables MDA, GSH, GSSG, GSH/GSSG and CAT. There was no significant difference in the mean effect for the MDA, GSH, GSSG, GSH/GSSG and CAT variables in the treated groups of rats with curcumin, rutin and the combination of curcumin and rutin. Conclusions: The oral administration of curcumin and rutin, single or combined, could reduce the oxidative stress and enhance the antioxidant status in hyperglycemic periodontitis rats.

Download Full-text

Evaluation of electroacupuncture for symptom modification in a rodent model of spontaneous osteoarthritis

Acupuncture in Medicine ◽

10.1177/09645284211020755 ◽

2021 ◽

pp. 096452842110207

Author(s):

Alexa P Spittler ◽

Maryam F Afzali ◽

Richard B Martinez ◽

Lauren A Culver ◽

Sarah E Leavell ◽

...

Keyword(s):

Rodent Model ◽

Knee Joints ◽

Control Group ◽

Disease Modification ◽

Inflammatory Biomarker ◽

Knee Oa ◽

Significant Difference ◽

Gene Transcripts ◽

Restricted Mobility ◽

Movement Parameters

Objective: Faced with the frustration of chronic discomfort and restricted mobility due to osteoarthritis (OA), many individuals have turned to acupuncture for relief. However, the efficacy of acupuncture for OA is uncertain, as much of the evidence is inconclusive. The purpose of this study was to evaluate electroacupuncture (EA) in a rodent model of OA such that conclusions regarding its effectiveness for symptom or disease modification could be drawn. Methods: Ten 12-month-old male Hartley guinea pigs—which characteristically have moderate to advanced OA at this age—were randomly assigned to receive EA for knee OA (n = 5) or anesthesia only (control group, n = 5). Treatments were performed three times weekly for 3 weeks, followed by euthanasia 2 weeks later. Gait analysis and enclosure monitoring were performed weekly to evaluate changes in movement. Serum was collected for inflammatory biomarker testing. Knee joints were collected for histology and gene expression. Results: Animals receiving EA had significantly greater changes in movement parameters compared to those receiving anesthesia only. There was a tendency toward decreased serum protein concentrations of complement component 3 (C3) in the EA group compared to the control group. Structural and antioxidant gene transcripts in articular cartilage were increased by EA. There was no significant difference in total joint histology scores between groups. Conclusion: This study provides evidence that EA has a positive effect on symptom, but not disease, modification in a rodent model of OA. Further investigations into mechanistic pathways that may explain the efficacy of EA in this animal model are needed.

Download Full-text

Histological Study On Using Ileal Submucosa In Repairing Urinary Bladder Defect In Adult Albino Rat

QJM ◽

10.1093/qjmed/hcab099.004 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

Eman Gomaa El Saeed ◽

Manal H Moussa ◽

Gehad A Hammouda ◽

Sahar M. M Omar

Keyword(s):

Urinary Bladder ◽

Histological Study ◽

Squamous Metaplasia ◽

Muscle Layer ◽

Control Group ◽

Albino Rats ◽

Adult Rats ◽

Group I ◽

Significant Difference ◽

Graft Area

Abstract Background Repairing urinary bladder (UB) defect by enterocystoplasty remains the gold standard surgical bladder reconstruction procedure to increase the capacity and compliance of dysfunctional bladders. However, many complications were recorded. Aim of the work This work aimed to compare the consequences of reconstruction of urinary bladder defect using untreated small intestinal submucosal (SIS) matrix versus seeded and unseeded decellularized SIS matrix. Material and Methods Fifty female albino rats were used in this study. The animals were divided into three groups: Group I (Control) included ten adult rats from which ileal tissue was obtained. Group II included ten adult rats in which their UB defect was repaired by untreated cellular SIS. Group III included twenty adult rats that were subdivided into two subgroups, 10 rats each; Subgroup IIIA where rats had their UB defect repaired by acellular SIS and subgroup IIIb where rats had their UB defect repaired by acellular SIS seeded with adipose mesenchymal stem cells (AMSCs).Ten young rats were used for preparation of AMSCs. Morphometric and statistical analysis were also performed. Results In rats where UB defect was repaired by untreated cellular SIS, the graft area showed loss of epithelial polarity, presence of intraepithelial cysts and occasional extension of urothelium to the outer surface forming fistula. There were areas of metaplasia with the appearance PAS positive cells. In the lamina propria, there was areas of lymphocytic infiltration together with significant increase in the collagen fiber deposition (p < 0.05). There was a significant decrease thickness of muscle layer as compared to control (p < 0.05). In rats where UB defect was repaired by acellular SIS, urothelium in the graft area showed occasional squamous metaplasia and often the urothelium extended to the deeper layers forming Brunn's nest. There was minimal muscle regeneration in the graft area. However, in rats where UB defect was repaired by acellular SIS seeded with AMSCs, the urothelium in the graft area was nearly similar to control group with uniform urothelium thickness, minimal collagen fibers deposition and thick muscle layer that showed no significant difference from the control (p > 0.05). Conclusion Acellular SIS seeded with AMSCs showed better results compared to non-seeded and cellular SIS in reconstructing urinary bladder defects.

Download Full-text

Effect of Tramadol on Sperm Profile of Male Albino Rats

Asian Journal of Research in Biochemistry ◽

10.9734/ajrb/2018/v3i429844 ◽

2019 ◽

pp. 1-6

Author(s):

I. S. Esua ◽

U. U. Uno ◽

U. B. Ekaluo

Keyword(s):

Sperm Motility ◽

Sperm Count ◽

Sperm Head ◽

Male Rats ◽

Control Group ◽

Albino Rats ◽

Dependent Manner ◽

Sperm Viability ◽

Albino Rat ◽

Significant Difference

Background and Aim: Tramadol is a potent analgesic effective in the treatment of mild to severe pains. However, the use of the drug can pose a threat to other organs and systems. Therefore, this study evaluated the effect of graded doses of tramadol on sperm profile of male albino rats. Materials and Methods: Eighteen male rats were divided into three groups (A, B and C) using completely randomized design (CRD) with six rats in each group. Rats in group A served as the control group and were given just food and water while groups B and C were given tramadol at 50 and 100 mg/kg body weight (BW) respectively, daily for the period of 65 days. The treatment was administered via oral gavage and at the end of the treatments, the rats were sacrificed. Immediately after sacrifice, a puncture was made in the epididymis with a sterile pin and examined for semen pH. The epididymes were processed for epididymal sperm motility, viability, count and sperm head abnormality. Results: There was no significant difference in the weight of testes and semen pH. Sperm viability, sperm motility, sperm count and weight of epididymes significantly reduced (p<0.05) in tramadol treated animals when compared with the control. Results also indicated statistically significant (p<0.05) increase in sperm head abnormalities in rats treated with tramadol when compared with the control. Conclusion: The results obtained from this study reveal that tramadol has negative effects on weight of epididymes, sperm count, sperm viability, sperm motility and sperm head abnormalities in male albino rat as mammalian models in a dose dependent manner.

Download Full-text

Growth of Olfactory Ensheathing Cells on Silk Fibroin Nanofibers

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.175-176.230 ◽

2011 ◽

Vol 175-176 ◽

pp. 230-235

Author(s):

Yi Xin Shen ◽

Peng Wu ◽

Zhi Hai Fan ◽

Feng Zhang ◽

Zheng Feng Lu ◽

...

Keyword(s):

Silk Fibroin ◽

Growth Factor Receptor ◽

Olfactory Ensheathing Cells ◽

Control Group ◽

Significant Difference ◽

Contrast Microscopy ◽

Ensheathing Cells ◽

Silk Fibroin Nanofibers ◽

Experimental Group ◽

Original Characteristic

Objective: To evaluate the growth of olfactory ensheathing cells (OECs) on the silk fibroin (SF) nanofibers scaffold. Methods: The purified OECs were cultured with poly-L-lysine (control group) and 1200 nm SF nanofibers (experimental group). The morphological features and growth characteristics of which were analyzed by phase contrast microscopy. Nerve growth factor receptor (NGFR) p75 were applied to identify OECs by immunostaining. SEM was used to observe the adherence and spreading of OECs on different substrates. MTT assay was performed to evaluate the proliferation activity of OECs both on the control and experimental scaffolds. Results: The isolated OECs reached confluence after 4-5 days of culture, which were stained for antibody NGFRp75(+). The morphology of OECs on the 1200 nm SF nanofibers was similar to that on the control group. The SEM clearly revealed the close interaction between the OECs and the nanofbers. The OECs on SF nanofibers still maintain its original characteristic phenotypes. The MTT showed that the most obvious proliferation was reached over 10 days. The differences of OD values between 1200 nm SF and PLL were significant at day 5, 7 (p < 0.05). However, there was no significant difference at day 10. Conclusion: SF nanofibers scaffold could support the growth of OECs, and may be a promising tissue-engineered scaffold for the repair of SCI.

Download Full-text

The Effects of Hyperbaric Oxygen Therapy on Experimental Colon Anastomosis After Preoperative Chemoradiotherapy

International Surgery ◽

10.9738/cc130.2 ◽

2013 ◽

Vol 98 (1) ◽

pp. 33-42 ◽

Cited By ~ 7

Author(s):

Ramazan Yildiz ◽

Mehmet Fatih Can ◽

Gokhan Yagci ◽

Taner Ozgurtas ◽

Metin Guden ◽

...

Keyword(s):

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Therapy ◽

Oxygen Therapy ◽

Preoperative Chemoradiotherapy ◽

Colon Resection ◽

Burst Pressure ◽

Control Group ◽

Albino Rats ◽

Colon Anastomosis ◽

Significant Difference

Abstract The aim of the present study was to investigate the effect of hyperbaric oxygen therapy (HBOT) on colon anastomosis after chemoradiotherapy (CRT). Sixty female Wistar-Albino rats were divided into 5 groups and underwent left colon resection and end-to-end anastomosis. CRT simulation was performed on 2 sham groups before the anastomosis, and 1 of these groups was administered additional postoperative HBOT. Two groups were administered CRT before the anastomosis, and 1 of them received additional postoperative HBOT. On postoperative day 5, all groups underwent relaparotomy; burst pressure was measured and samples were obtained for histopathologic and biochemical analysis. There was a significant weight loss in the CRT groups and postoperative HBOT had an improving effect. Significantly decreased burst pressure values increased up to the levels of the controls after HBOT. Hydroxyproline levels were elevated in all groups compared to the control group. Hydroxyproline levels decreased with HBOT after CRT. No significant difference was observed between the groups regarding fibrosis formation at the anastomosis site. However, regression was observed in fibrosis in the group receiving HBOT after CRT. Preoperative CRT affected anastomosis and wound healing unfavorably. These unfavorable effects were alleviated by postoperative HBOT. HBOT improved the mechanical and biochemical parameters of colon anastomosis in rats.

Download Full-text

Protective effect of N-acetylcysteine, caffeic acid and vitamin E on doxorubicin hepatotoxicity

Human & Experimental Toxicology ◽

10.1177/0960327107076885 ◽

2007 ◽

Vol 26 (6) ◽

pp. 519-525 ◽

Cited By ~ 36

Author(s):

A. Gokcimen ◽

A. Cim ◽

H.T. Tola ◽

D. Bayram ◽

A. Kocak ◽

...

Keyword(s):

Vitamin E ◽

Caffeic Acid ◽

Control Group ◽

Albino Rats ◽

Central Vein ◽

Protective Effects ◽

Injection Group ◽

Hepatocellular Damage ◽

Group I ◽

Significant Difference

The aim of this study was to compare the possible protective effects of N-acetylcysteine (NAC), caffeic acid (CAPE) and vitamin E (Vit-E) on doxorubicin-induced hepatotoxicity. Thirty-two male Wistar albino rats, weighing between 250 and 350 g were supplied and randomly divided into five groups. Animals in study groups were pretreated with a single dose of doxorubicin (Dox), which was administered intraperitoneally (i.p.). Control group (Group I) was treated with intraperitoneal saline injection. Group II did not received any antioxidant agent after the injection. Group III and Group IV were given CAPE and intraperitoneal vitamin E injection for eight days, respectively. Group V received NAC for eight days. The study was finished after 10 days. Tissue samples were collected from all animals and histopathological examination was performed. There was statistically significant difference between the experiment groups and controls by means of mononuclear cell infiltration and diameters of hepatic sinusoid, terminal hepatic venule (central vein) and portal area (portal canal). Changes related with hepatocellular damage were more prominent, whereas there was no significant difference between Dox and NAC given groups histopathologically. It was observed that structural changes were regressed after CAPE administration. However, this recovery was more prominent in vitamin E given group. These findings suggest that Dox induced liver damage could be efficiently reversed by vitamin E administration. It has been found that CAPE, but not NAC has protective effects on Dox-induced hepatocellular damage. Human & Experimental Toxicology (2007) 26, 519—525

Download Full-text

Wear of Resin Composites Polymerized by Conventional Halogen Light Curing and Light Emitting Diodes Curing Units

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.288-289.645 ◽

2005 ◽

Vol 288-289 ◽

pp. 645-648

Author(s):

Hwan Kim ◽

Sung Ho Park ◽

I.Y. Jung ◽

S.B. Jeon ◽

Kwon Yong Lee

Keyword(s):

Light Emitting Diodes ◽

Composite Resins ◽

Dental Composite ◽

Zirconia Ceramic ◽

Resin Composites ◽

Light Emitting ◽

Dental Composite Resins ◽

Halogen Light ◽

Significant Difference ◽

Light Curing

In this study, the wear characteristics of five different dental composite resins cured by conventional halogen light and LED light sources were investigated. Five different dental composite resins of Surefil, Z100, Dyract AP, Fuji II LC and Compoglass were worn against a zirconia ceramic ball using a pin-on-disk type wear tester with 15 N contact force in a reciprocal sliding motion of sliding distance of 10 mm/cycle at 1Hz under the room temperature dry condition. The wear variations of dental composite resins were linearly increased as the number of cycles increased. It was observed that the wear resistances of these specimens were in the order of Dyract AP > Surefil > Compoglass > Z100 > Fuji ı LC. On the morphological observations by SEM, the large crack formation on the sliding track of Fuji ıLC specimen was the greatest among all resin composites. Dyract AP showed less wear with few surface damage. There is no significant difference in wear performance between conventional halogen light curing and light emitting diodes curing sources. It indicates that a light emitting diodes (LED) source can replace a halogen light source as curing unit for composite resin restorations.

Download Full-text

Evaluation of the bond strength of orthodontic brackets and the degree of polymerisation and microhardness of an orthodontic adhesive using polyvawe light curing unit and varying light densities

Brazilian Dental Science ◽

10.14295/bds.2020.v23i4.2044 ◽

2020 ◽

Vol 23 (4) ◽

pp. 9p

Author(s):

Nuri Mert Topbasi ◽

Yasin Atakan Benkli

Keyword(s):

Bond Strength ◽

Light Emitting Diode ◽

Control Group ◽

Bond Failure ◽

Adhesive Resin ◽

Mean Values ◽

Light Emitting ◽

Significant Difference ◽

Light Curing

Objective: This study evaluates the shear bond strength (SBS) and Vickers micro-hardness (VHN) of the orthodontic adhesive resin Transbond XT using thermocycled samples. The study also measures the degree of cure by poliwave light curing unit Valo. Materials and Methods: 135 brackets were bonded for the SBS test, and 90 disc-shaped orthodontic adhesive resins were prepared for the VHN test. The measurements were taken either immediately or after 500 or 10.0000 thermocycling. The SBS and VHN test data were statistically evaluated using a Two-way ANOVA and Tukey multiple comparison tests. The degree of conversion (DC) was measured by FTIR. Results: The 10-sec polymerized control group (57.08) and the 10.000 termocycled samples (55.96) had the highest VHN scores. On the other hand, the 3 second polymerized and the 10.000 termocycled samples (39.22) had the lowest VHN scores. There was no significant difference in the bracket SBS values between the termocycled and immediate groups. An evaluation of the bond failure surfaces revealed that the ARI scores did not differ between the immediate and the thermo-cycled groups according to the power modes. The FTIR results revealed that the lowest DC was seen in the 3 second light-cured sample. Conclusion: Transbond XT provided sufficient bonding in terms of microhardness and DC under in vitro bracket bonding conditions after 10 and 8 seconds of Poliwave LED curing. However, low mean values were seen in the groups that received Poliwave LED curing for only 3 seconds. KEYWORDSARI score; FTIR; Light-Emitting Diode; Orthodontics; Thermocycling.

Download Full-text

Effect of single clove garlic on clinical symptoms of photoaging in wistar rats exposed with UV-B

Bali Anatomy Journal ◽

10.36675/baj.v2i2.28 ◽

2019 ◽

Vol 2 (2) ◽

pp. 40-44

Author(s):

Nyoman Pratiwi Hapsari Dewi ◽

I Gusti Ayu Dewi Ratnayanti ◽

I Gusti Kamasan Nyoman Arijana ◽

Ni Made Linawati

Keyword(s):

Clinical Symptom ◽

Wistar Rats ◽

Clinical Symptoms ◽

Light Exposure ◽

Premature Aging ◽

The Other ◽

Control Group ◽

Control Group Design ◽

Significant Difference ◽

Male Wistar Rats

Background: Photoaging is a premature aging that occurs on skin due to the ultraviolet light exposure that causes the emergence of clinical symptoms, one of which is wrinkle. One kind of material that can prevent photoaging is antioxidant. A single clove garlic has the highest antioxidant activity compared to the other materials. Aim: This research aimed at investigating the effectivity of single clove garlic in inhibiting the clinical symptom of photoaging. Method: This research was an experimental research, utilizing pre-post-test control group design. The used sample were 30 male wistar rats which were divided into 6 experimental groups. All groups were exposed to UV-B light with the amount of 840 mJ/cm2. Control group (P1) was only exposed to UV-B while the other groups were treated with placebo cream (P2), sunblock (P3), 5% garlic cream (P4), 10% garlic cream (P5) and 20% garlic cream (P6) respectively. The clinical symptom in the form of wrinkle was observed by using dermascope and the observations were categorized based on Glogau Scale. The statistical analysis utilized Wilcoxon and Kruskal Wallis test which was followed by Mann Whitney test. Result: The result of the research showed that there was significant difference on the apparent wrinkle on P1 group and P3, P5 and P6. Meanwhile the comparison between P1, P2 and P4 was not significant. Conclusion: The single clove garlic extract can prevent photoaging and has the similar protective effect for the skin as of sunblock.

Download Full-text