New Bactericide Orthodonthic Archwire: NiTi with Silver Nanoparticles

A potential new bactericide treatment for NiTi orthodontic archwires based in the electrodeposition of silver nanoparticles on the surface was studied. Twenty-five archwires were treated by electrodeposition, obtaining nanoparticles of silver embedded on the archwire surface. These were evaluated in order to investigate the possible changes on the superelastic characteristics (critical temperatures and stresses), the nickel ion release, and the bacteria culture behavior. The chemical composition was analyzed by Energy Dispersive X-Ray Spectroscopy-microanalysis; the singular temperatures of the martensitic transformation were obtained by a flow calorimeter. Induced martensitic transformation stresses were obtained by mechanical testing apparatus. Nickel ion release was analyzed by inductively coupled plasma-mass spectrometry (ICP-MS) equipment using artificial saliva solution at 37 °C. Bacterial tests were studied with the most used oral bacterial strains: Streptococcus sanguinis and Lactobacillus salivarius. NiTi samples were immersed in bacterial suspensions for 2 h at 37 °C. Adhered bacteria were separated and seeded on agar plates: Tood-Hewitt (TH) and Man-Rogosa-Sharpe (MRS) for S. sanguinis and for L.salivarius, respectively. These were then incubated at 37 °C for 1 day and the colonies were analyzed. The results showed that the transformation temperatures and the critical stresses have not statistically significant differences. Likewise, nickel ion release at different immersion times in saliva at 37 °C does not present changes between the original and treated with silver nanoparticles archwires. Bacteria culture results showed that the reduction of the bacteria due to the presence to the nanoparticles of silver is higher than 90%. Consequently, the new treatment with nanoparticles of silver could be a good candidate as bactericidic orthodontic archwire.

Download Full-text

Pengaruh Material Konservasi Kolostrum terhadap Pelepasan Ion Ni

Jurnal Teknosains ◽

10.22146/teknosains.37727 ◽

2019 ◽

Vol 9 (1) ◽

pp. 12

Author(s):

Grahita Aditya ◽

Hikmah Nuraini ◽

Whinahyu Aji Sekarini

Keyword(s):

Sodium Fluoride ◽

Inductively Coupled Plasma ◽

Artificial Saliva ◽

Optical Emission ◽

Ion Release ◽

Nickel Ion ◽

Orthodontic Bracket ◽

Nickel Ions ◽

Type Iv ◽

Significant Difference

Nickel in orthodontic bracket will be released immediately in the oral cavity. The release of Ni ion may trigger type IV hypersensitivity reaction. Colostrum has a high content of lactoferrin which can inhibit T cells proliferation after nickel sensitization, and decrease oxidative stress. Adding potassium sorbat 0.5%, or fermentation of colostrum may extend colostrum storage. The purpose of this research is to know the difference of nickel ion release in colostrum with 0.5% potassium sorbat addition and fermented colostrum. Methods of the study were laboratory experimental with four groups, colostrum and 0.5% potassium sorbat, fermented colostrum, sodium fluoride and artificial saliva. The sample was incubated in 40°C for 7 days. Nickel ions was measured using an inductively coupled plasma-optical emission spectrophotometry (ICP-OES Perlun Elmer Optima 8300®). Ni ions release differences were tested using the Kruskall-Wallis and Mann Whitney test.The lowest rate of Ni ion release wasfound in the colostrum group with 0.5% potassium sorbat followed by the fermented colostrum group. Kruskal-Wallis test no significant difference (p> 0,05) in each study group. The Mann Whitney test found significant differences between colostrum solution with 0.5% potassium sorbat and sodium fluoride, and also artificial saliva. The results concluded that colostrum with 0.5% potassium sorbat inhibit the release of Ni ions.

Download Full-text

Synthesis, characterization and application of green seaweed mediated silver nanoparticles (AgNPs) as antibacterial agents for water disinfection

Water Science & Technology ◽

10.2166/wst.2018.292 ◽

2018 ◽

Vol 78 (1) ◽

pp. 235-246 ◽

Cited By ~ 7

Author(s):

D. Dixit ◽

D. Gangadharan ◽

K. M. Popat ◽

C. R. K. Reddy ◽

M. Trivedi ◽

...

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Inductively Coupled Plasma ◽

Antibacterial Agents ◽

Bacterial Count ◽

Water Disinfection ◽

Bacterial Strains ◽

Vis Spectroscopy ◽

Green Seaweed

Abstract A simple and eco-friendly method for the synthesis of hybrid bead silver nanoparticles (AgNPs) employing the aqueous extract derived from natural and renewable source namely tropical benthic green seaweed Ulva flexuosa was developed. This route involves the reduction of Ag+ ions anchored onto macro porous methacrylic acid copolymer beads to AgNPs for employing them as antibacterial agents for in vitro water disinfection. The seaweed extract itself acts as a reducing and stabilizing agent and requires no additional surfactant or capping agent for forming the AgNPs. The nanoparticles were analyzed using high-resolution transmission electron microscopy, UV–Vis spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray analysis and inductively coupled plasma optical emission spectroscopy. The study elucidates that such biologically synthesized AgNPs exhibit potential antibacterial activity against two Gram positive (Bacillus subtilis, Staphylococcus aureus) and two Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacterial strains tested. The bacterial count in treated water was reduced to zero for all the strains. Atomic force microscopy was performed to confirm the pre- and post-state of the bacteria with reference to their treatment with AgNPs. Attributes like facile environment-friendly procedure, stability and high antibacterial potency propel the consideration of these AgNPs as promising antibacterial entities.

Download Full-text

Metal ion release from silver soldering and laser welding caused by different types of mouthwash

The Angle Orthodontist ◽

10.2319/050914-335.1 ◽

2014 ◽

Vol 85 (4) ◽

pp. 665-672 ◽

Cited By ~ 4

Author(s):

Ayse Tuygun Erdogan ◽

Didem Nalbantgil ◽

Feyza Ulkur ◽

Fikrettin Sahin

Keyword(s):

Laser Welding ◽

Metal Ions ◽

Sodium Fluoride ◽

Inductively Coupled Plasma ◽

Metal Ion ◽

Artificial Saliva ◽

Ion Release ◽

Sem Images ◽

Metal Ion Release ◽

Group Comparisons

ABSTRACT Objective: To compare metal ion release from samples welded with silver soldering and laser welding when immersed into mouthwashes with different ingredients. Materials and Methods: A total of 72 samples were prepared: 36 laser welded and 36 silver soldered. Four samples were chosen from each subgroup to study the morphologic changes on their surfaces via scanning electron microscopy (SEM). Each group was further divided into four groups where the samples were submerged into mouthwash containing sodium fluoride (NaF), mouthwash containing sodium fluoride + alcohol (NaF + alcohol), mouthwash containing chlorhexidine (CHX), or artificial saliva (AS) for 24 hours and removed thereafter. Subsequently, the metal ion release from the samples was measured with inductively coupled plasma mass spectrometry (ICP-MS). The metal ion release among the solutions and the welding methods were compared. The Kruskal-Wallis and analysis of variance (ANOVA) tests were used for the group comparisons, and post hoc Dunn multiple comparison test was utilized for the two group comparisons. Results: The level of metal ion release from samples of silver soldering was higher than from samples of laser welding. Furthermore, greater amounts of nickel, chrome, and iron were released from silver soldering. With regard to the mouthwash solutions, the lowest amounts of metal ions were released in CHX, and the highest amounts of metal ions were released in NaF + alcohol. SEM images were in accord with these findings. Conclusions: The laser welding should be preferred over silver soldering. CHX can be recommended for patients who have welded appliances for orthodontic reasons.

Download Full-text

Nickel ion release from dental alloys in two different mouthwashes

Journal of Dental Research Dental Clinics Dental Prospects ◽

10.15171/joddd.2019.003 ◽

2019 ◽

Vol 13 (1) ◽

pp. 19-23 ◽

Cited By ~ 2

Author(s):

Karim Jafari ◽

Saeed Rahimzadeh ◽

Somayeh Hekmatfar

Keyword(s):

Inductively Coupled Plasma ◽

Control Group ◽

Distilled Water ◽

Dental Alloys ◽

Ion Release ◽

Nickel Ion ◽

Inductively Coupled ◽

Nickel Chromium ◽

Significant Difference ◽

History Of

Background. Mouthwashes are widely used as adjuncts to mechanical oral hygiene procedures. Nonetheless, there is little information regarding the effect of various mouthwashes on the amount of ions released from the nickel-chromium (Ni‒Cr) alloys used in the fabrication of fixed prostheses. Therefore, the present study was conducted to evaluate the effect of two types of mouthwash on the release of Ni ions from dental alloys. Methods. Forty-two disk-shaped specimens were prepared with a diameter of 10 mm and a height of 2 mm. Two mouthwashes were examined in this study: Oral B and Listerine. A control group was also considered using distilled water. Each Ni‒Cr disk was immersed in the mouthwashes and distilled water in polypropylene test tubes, and then incubated at 37°C to simulate the oral temperature. After 45 days of incubation, the samples were tested for Ni ions using inductively coupled plasma mass spectroscopy. Data were analyzed using ANOVA. Results. In the Halita group subjects exhibited 2.04±0.65 reduction in OLS. OLS reduction in the chlorhexidine group was 1.95±0.74. Statistical analysis showed no significant difference between the two groups (P>0.05). Conclusion. As the results indicated, the amount of ion release was within the safe limits in the two experimental groups. However, it is recommended that prescribe Listerine mouthwash should not be prescribed for the patients with a history of Ni allergy.

Download Full-text

Metallic ion release from biocompatible cobalt-based alloy

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq130813039d ◽

2014 ◽

Vol 20 (4) ◽

pp. 571-577 ◽

Cited By ~ 5

Author(s):

Ivana Dimic ◽

Ivana Cvijovic-Alagic ◽

Ivana Kostic ◽

Aleksandra Peric-Grujic ◽

Marko Rakin ◽

...

Keyword(s):

Inductively Coupled Plasma ◽

Artificial Saliva ◽

High Strength ◽

Metallic Ions ◽

Ion Release ◽

Inductively Coupled ◽

Ph Values ◽

Metallic Biomaterials ◽

Icp Ms ◽

Research Findings

Metallic biomaterials, which are mainly used for the damaged hard tissue replacements, are materials with high strength, excellent toughness and good wear resistance. The disadvantages of metals as implant materials are their susceptibility to corrosion, the elastic modulus mismatch between metals and human hard tissues, relatively high density and metallic ion release which can cause serious health problems. The aim of this study was to examine metallic ion release from Co-Cr-Mo alloy in artificial saliva. In that purpose, alloy samples were immersed into artificial saliva with different pH values (4.0, 5.5 and 7.5). After a certain immersion period (1, 3 and 6 weeks) the concentrations of released ions were determined using Inductively Coupled Plasma - Mass Spectrophotometer (ICP-MS). The research findings were used in order to define the dependence between the concentration of released metallic ions, artificial saliva pH values and immersion time. The determined released metallic ions concentrations were compared with literature data in order to describe and better understand the phenomenon of metallic ion release from the biocompatible cobalt-based alloy.

Download Full-text

Release of nickel ions and changes in surface microstructure of stainless steel archwire after immersion in tomato and orange juice

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/912/1/012018 ◽

2021 ◽

Vol 912 (1) ◽

pp. 012018

Author(s):

H F Lubis ◽

G H Simamora

Keyword(s):

Stainless Steel ◽

Orange Juice ◽

Artificial Saliva ◽

Tomato Juice ◽

Surface Microstructure ◽

Orthodontic Appliances ◽

Ion Release ◽

Nickel Ion ◽

Nickel Ions ◽

Significant Difference

Abstract Stainless steel archwire is an important component of orthodontic appliances that have the potential to corrode. Consumption of foods and beverages with a low pH, such as fruit-based juices, can trigger the release of nickel ions in stainless steel archwire. This study aimed to determine the difference in the amount of nickel ions release and the surface microstructure of stainless steel archwire after immersed in tomato and orange juice. The sample used is stainless steel archwire with a diameter of 0.016 inches and length of 5 cm immersed in 15 ml of solution and then stored at 37°C in an incubator for 24 hours. The samples were divided into three groups (immersed in tomato juice,orange juice and artificial saliva), each group consisted of 9 samples. The solution was tested using an Inductively Coupled Plasma Mass Spectrometer (ICP-MS) to determine the number of nickel ions released. The archwire surface microstructure was tested using a Scanning Electron Microscope (SEM). The results showed that the average amount of nickel ion release in orange juice is more than tomato juice. There was a significant difference between the amount of nickel ion released and surface microstructure on stainless steel archwire after being immersed in tomato and orange juice.

Download Full-text

Printable Resin Modified by Grafted Silver Nanoparticles for Preparation of Antifouling Microstructures with Antibacterial Effect

Polymers ◽

10.3390/polym13213838 ◽

2021 ◽

Vol 13 (21) ◽

pp. 3838

Author(s):

Hazem Idriss ◽

Roman Elashnikov ◽

Silvie Rimpelová ◽

Barbora Vokatá ◽

Petr Haušild ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Effect ◽

Low Cost ◽

Antibacterial Properties ◽

Silver Ions ◽

Polymerization Process ◽

Resin Matrix ◽

Bacterial Strains ◽

Ion Release ◽

3D Printed

The usage of three-dimensional (3D) printed materials in many bioapplications has been one of the fastest-growing sectors in the nanobiomaterial industry in the last couple of years. In this work, we present a chemical approach for grafting silver nanoparticles (AgNPs) into a resin matrix, which is convenient for 3D printing. In this way, the samples can be prepared and are able to release silver ions (Ag+) with excellent antibacterial effect against bacterial strains of E. coli and S. epidermidis. By the proposed process, the AgNPs are perfectly mixed and involved in the polymerization process and their distribution in the matrix is homogenous. It was also demonstrated that this approach does not affect the printing resolution and the resin is therefore suitable for the construction of microstructures enabling controlled silver ion release and antifouling properties. At the same time the physical properties of the material, such as viscosity and elasticity modulus are preserved. The described approach can be used for the fabrication of facile, low-cost 3D printed resin with antifouling-antibacterial properties with the possibility to control the release of Ag+ through microstructuring.

Download Full-text

“To Be Microbiocidal and Not to Be Cytotoxic at the Same Time…”—Silver Nanoparticles and Their Main Role on the Surface of Titanium Alloy Implants

Journal of Clinical Medicine ◽

10.3390/jcm8030334 ◽

2019 ◽

Vol 8 (3) ◽

pp. 334 ◽

Cited By ~ 8

Author(s):

Aleksandra Radtke ◽

Marlena Grodzicka ◽

Michalina Ehlert ◽

Tomasz Jędrzejewski ◽

Magdalena Wypij ◽

...

Keyword(s):

Silver Nanoparticles ◽

Titanium Alloy ◽

Inductively Coupled Plasma ◽

Chemical Vapor ◽

Biological Properties ◽

Metallic Silver ◽

Ionization Mass ◽

Main Role ◽

Silver Particles ◽

Ion Release

The chemical vapor deposition (CVD) method has been used to produce dispersed silver nanoparticles (AgNPs) on the surface of titanium alloy (Ti6Al4V) and nanotubular modified titanium alloys (Ti6Al4V/TNT5), leading to the formation of Ti6Al4V/AgNPs and Ti6Al4V/TNT5/AgNPs systems with different contents of metallic silver particles. Their surface morphology and silver particles arrangement were characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDS), and atomic force microscopy (AFM). The wettability and surface free energy of these materials were investigated on the basis of contact angle measurements. The degree of silver ion release from the surface of the studied systems immersed in phosphate buffered saline solution (PBS) was estimated using inductively coupled plasma ionization mass spectrometry (ICP-MS). The biocompatibility of the analyzed materials was estimated based on the fibroblasts and osteoblasts adhesion and proliferation, while their microbiocidal properties were determined against Gram-positive and Gram-negative bacteria, and yeasts. The results of our works proved the high antimicrobial activity and biocompatibility of all the studied systems. Among them, Ti6Al4V/TNT5/0.6AgNPs contained the lowest amount of AgNPs, but still revealed optimal biointegration properties and high biocidal properties. This is the biomaterial that possesses the desired biological properties, in which the potential toxicity is minimized by minimizing the number of silver nanoparticles.

Download Full-text

Release of nickel ion and surface microstructure of NiTi archwire after immersion in tomato and orange juice

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/912/1/012017 ◽

2021 ◽

Vol 912 (1) ◽

pp. 012017

Author(s):

H F Lubis ◽

Y A Purba

Keyword(s):

Orange Juice ◽

Inductively Coupled Plasma ◽

Surface Microstructure ◽

Ion Release ◽

Nickel Ion ◽

Inductively Coupled ◽

Significant Difference ◽

The Difference ◽

Group 2 ◽

Group 1

Abstract One of wires commonly used in orthodontic treatment is nickel-titanium (NiTi) archwire. NiTi archwire has the potential to release ions that can cause allergic and cytotoxic reactions. This study aimed to specify the difference in the amount of nickel ion release and surface microstructure of NiTi archwires after immersing in tomato and orange juice. NiTi archwire with a diameter of 0.016 inches and a length of 5 cm was used as the sample, which was immersed in 15 ml of solution and then stored at 37°C in an incubator for 24 hours. The samples were divided into two tretment groups (immersed in tomato and orange juice), each with nine samples. The immersion solution was tested for ion release using an Inductively Coupled Plasma–Mass Spectrometer. The microstructure of the wire surface was examined using a scanning electron microscope (SEM). The results revealed that group 1 has higher average amount of nickel ion release than group 2 and control. SEM result showed that the surface microstructure of the NiTi archwire in group 1 is roughest. There was a significant difference between the amount of nickel ion released and surface microstructure on NiTi archwire after being immersed in tomato and orange juice.

Download Full-text

Reduction of Nickel Ion Release on a TiO2 Coated onto an Orthodontic Wire

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.789.204 ◽

2013 ◽

Vol 789 ◽

pp. 204-209

Author(s):

Sungging Pintowantoro ◽

Yuli Setiyorini

Keyword(s):

Corrosion Resistance ◽

Inductively Coupled Plasma ◽

Advanced Oxidation Process ◽

The Body ◽

Wire Surface ◽

Ion Release ◽

Nickel Ion ◽

Inductively Coupled ◽

Orthodontic Wire ◽

Increase Corrosion Resistance

NiTi is alloy that has unique properties include shape memory dan superelasticity. However, it has disadvantages which cause allergic and toxicity to the body from the Ni release. In order to reduce Ni release and increase corrosion resistance an Electropolishing Pretreatment-Photoelectrocatalytic Oxidation (EP-PEO) and Advanced Oxidation Process (AOP) coating were synthesized in order to produce TiO2. Scanning Electron Microscope (SEM) was depicted various morphologies of NiTi orthodontic wire surface. Ni release experiment in various saliva by using Inductively Coupled Plasma (ICP) was shown reducing Ni release significantly both in saliva pH 3 dan pH 6.25 in AOP coating for 2 months monitoring. This results confirmed that the Ni release was evidently decreased and the corrosion resistance significantly improved after coated with AOP.

Download Full-text