scholarly journals Accelerated Particle Separation in a DLD Device at Re > 1 Investigated by Means of µPIV

Micromachines ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 768 ◽  
Author(s):  
Jonathan Kottmeier ◽  
Maike Wullenweber ◽  
Sebastian Blahout ◽  
Jeanette Hussong ◽  
Ingo Kampen ◽  
...  

A pressure resistant and optically accessible deterministic lateral displacement (DLD) device was designed and microfabricated from silicon and glass for high-throughput fractionation of particles between 3.0 and 7.0 µm comprising array segments of varying tilt angles with a post size of 5 µm. The design was supported by computational fluid dynamic (CFD) simulations using OpenFOAM software. Simulations indicated a change in the critical particle diameter for fractionation at higher Reynolds numbers. This was experimentally confirmed by microparticle image velocimetry (µPIV) in the DLD device with tracer particles of 0.86 µm. At Reynolds numbers above 8 an asymmetric flow field pattern between posts could be observed. Furthermore, the new DLD device allowed successful fractionation of 2 µm and 5 µm fluorescent polystyrene particles at Re = 0.5–25.

2016 ◽  
Vol 13 (116) ◽  
pp. 20160068 ◽  
Author(s):  
Gen Li ◽  
Ulrike K. Müller ◽  
Johan L. van Leeuwen ◽  
Hao Liu

Larvae of bony fish swim in the intermediate Reynolds number ( Re ) regime, using body- and caudal-fin undulation to propel themselves. They share a median fin fold that transforms into separate median fins as they grow into juveniles. The fin fold was suggested to be an adaption for locomotion in the intermediate Reynolds regime, but its fluid-dynamic role is still enigmatic. Using three-dimensional fluid-dynamic computations, we quantified the swimming trajectory from body-shape changes during cyclic swimming of larval fish. We predicted unsteady vortices around the upper and lower edges of the fin fold, and identified similar vortices around real larvae with particle image velocimetry. We show that thrust contributions on the body peak adjacent to the upper and lower edges of the fin fold where large left–right pressure differences occur in concert with the periodical generation and shedding of edge vortices. The fin fold enhances effective flow separation and drag-based thrust. Along the body, net thrust is generated in multiple zones posterior to the centre of mass. Counterfactual simulations exploring the effect of having a fin fold across a range of Reynolds numbers show that the fin fold helps larvae achieve high swimming speeds, yet requires high power. We conclude that propulsion in larval fish partly relies on unsteady high-intensity vortices along the upper and lower edges of the fin fold, providing a functional explanation for the omnipresence of the fin fold in bony-fish larvae.


2021 ◽  
Vol 385 ◽  
pp. 418-433
Author(s):  
S.R. Reinecke ◽  
S. Blahout ◽  
T. Rosemann ◽  
B. Kravets ◽  
M. Wullenweber ◽  
...  

Author(s):  
Brian Senf ◽  
Kawkab Ahasan ◽  
Jong-Hoon Kim

Abstract Deterministic Lateral Displacement (DLD) is an inertial size-based particle separation technique with great possibilities for use with biological sample preparation. Recently it has been shown that particle shift of a DLD is highly dependent on the Reynolds number. Additionally, particle trajectory has been characterized in a high throughput airfoil array DLD with varying Angle of Attack (AoA) in Deionized water. The AoA can be shifted negatively assisting in particle trajectory increases at low Reynolds numbers. With variations in fluid viscosity, particle trajectories compared to Reynolds value should theoretically have a constant and similar slope. In this work, various viscosities are tested in a DLD with a neutral and negative AoA to eventually characterize non-Newtonian fluids within a DLD. Due to higher viscosities increasing the internal pressure of the device, the negative AoA DLD shows promising results at higher range viscosities due to its ability to shift particles at lower Reynolds numbers.


Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 263
Author(s):  
Tianlong Zhang ◽  
Yigang Shen ◽  
Ryota Kiya ◽  
Dian Anggraini ◽  
Tao Tang ◽  
...  

Continuous microfluidic focusing of particles, both synthetic and biological, is significant for a wide range of applications in industry, biology and biomedicine. In this study, we demonstrate the focusing of particles in a microchannel embedded with glass grooves engraved by femtosecond pulse (fs) laser. Results showed that the laser-engraved microstructures were capable of directing polystyrene particles and mouse myoblast cells (C2C12) towards the center of the microchannel at low Reynolds numbers (Re < 1). Numerical simulation revealed that localized side-to-center secondary flows induced by grooves at the channel bottom play an essential role in particle lateral displacement. Additionally, the focusing performance proved to be dependent on the angle of grooves and the middle open space between the grooves based on both experiments and simulation. Particle sedimentation rate was found to critically influence the focusing of particles of different sizes. Taking advantage of the size-dependent particle lateral displacement, selective focusing of micrometer particles was demonstrated. This study systematically investigated continuous particle focusing in a groove-embedded microchannel. We expect that this device will be used for further applications, such as cell sensing and nanoparticle separation in biological and biomedical areas.


Author(s):  
Johannes Gradl ◽  
Florian Schwertfirm ◽  
Hans-Christoph Schwarzer ◽  
Hans-Joachim Schmid ◽  
Michael Manhart ◽  
...  

Mixing and consequently fluid dynamic is a key parameter to tailor the particle size distribution (PSD) in nanoparticle precipitation. Due to fast and intensive mixing a static T-mixer configuration is capable for synthesizing continuously nanoparticles. The flow and concentration field of the applied mixer is investigated experimentally at different flow rates by Particle Image Velocimetry (PIV) and Laser Induced Fluorescence (LIF). Due to the PIV measurements the flow field in the mixer was characterized qualitatively and the mixing process itself is quantified by the subsequent LIF-measurements. A special feature of the LIF set up is to detect structures in the flow field, which are smaller than the Batchelor length. Thereby a detailed insight into the mixing process in a static T-Mixer is given. In this study a CFD-based approach using Direct Numerical Simulation (DNS) in combination with the solid formation kinetics solving population balance equations (PBE) is applied, using barium sulfate as modeling material. A Lagrangian Particle Tracking strategy is used to couple the flow field information with a micro mixing model and with the classical theory of nucleation. We found that the DNS-PBE approach including macro and micro mixing, combined with the population balance is capable of predicting the full PSD in nanoparticle precipitation for different operating parameters. Additionally to the resulting PSD, this approach delivers a 3D-information about all running subprocesses in the mixer, i.e. supersaturation built-up or nucleation, which is visualized for different process variables.


Author(s):  
F. E. Ames ◽  
L. A. Dvorak

The objective of this research has been to experimentally investigate the fluid dynamics of pin fin arrays in order to clarify the physics of heat transfer enhancement and uncover problems in conventional turbulence models. The fluid dynamics of a staggered pin fin array have been studied using hot wire anemometry with both single and x-wire probes at array Reynolds numbers of 3000; 10,000; and 30,000. Velocity distributions off the endwall and pin surface have been acquired and analyzed to investigate turbulent transport in pin fin arrays. Well resolved 3-D calculations have been performed using a commercial code with conventional two-equation turbulence models. Predictive comparisons have been made with fluid dynamic data. In early rows where turbulence is low, the strength of shedding increases dramatically with increasing in Reynolds numbers. The laminar velocity profiles off the surface of pins show evidence of unsteady separation in early rows. In row three and beyond laminar boundary layers off pins are quite similar. Velocity profiles off endwalls are strongly affected by the proximity of pins and turbulent transport. At the low Reynolds numbers, the turbulent transport and acceleration keep boundary layers thin. Endwall boundary layers at higher Reynolds numbers exhibit very high levels of skin friction enhancement. Well resolved 3-D steady calculations were made with several two-equation turbulence models and compared with experimental fluid mechanic and heat transfer data. The quality of the predictive comparison was substantially affected by the turbulence model and near wall methodology.


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