scholarly journals Focusing of Particles in a Microchannel with Laser Engraved Groove Arrays

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 263
Author(s):  
Tianlong Zhang ◽  
Yigang Shen ◽  
Ryota Kiya ◽  
Dian Anggraini ◽  
Tao Tang ◽  
...  
Keyword(s):  
Open Space ◽  
Lateral Displacement ◽  
Reynolds Numbers ◽  
Secondary Flows ◽  
Particle Sedimentation ◽  
Nanoparticle Separation ◽  
Wide Range ◽  
Fs Laser ◽  
Channel Bottom ◽  
Myoblast Cells

Continuous microfluidic focusing of particles, both synthetic and biological, is significant for a wide range of applications in industry, biology and biomedicine. In this study, we demonstrate the focusing of particles in a microchannel embedded with glass grooves engraved by femtosecond pulse (fs) laser. Results showed that the laser-engraved microstructures were capable of directing polystyrene particles and mouse myoblast cells (C2C12) towards the center of the microchannel at low Reynolds numbers (Re < 1). Numerical simulation revealed that localized side-to-center secondary flows induced by grooves at the channel bottom play an essential role in particle lateral displacement. Additionally, the focusing performance proved to be dependent on the angle of grooves and the middle open space between the grooves based on both experiments and simulation. Particle sedimentation rate was found to critically influence the focusing of particles of different sizes. Taking advantage of the size-dependent particle lateral displacement, selective focusing of micrometer particles was demonstrated. This study systematically investigated continuous particle focusing in a groove-embedded microchannel. We expect that this device will be used for further applications, such as cell sensing and nanoparticle separation in biological and biomedical areas.

NUMERICAL CHARACTERIZATION OF THE FLOW FIELD IN A FOUR-GENERATION AIRWAY

2008 ◽  
Vol 08 (01) ◽  
pp. 55-74 ◽  
Author(s):  
T. C. LAI ◽  
Y. S. MORSI ◽  
M. SINGH
Keyword(s):  
Reynolds Numbers ◽  
Bronchial Tree ◽  
Secondary Flows ◽  
Fourth Generation ◽  
Numerical Characterization ◽  
Wide Range ◽  
Symmetrical Model ◽  
Computational Fluid Dynamics Cfd ◽  
Insight Into

In this paper, various aspects of respiratory airflow generated from the branching network of tubes that make up the tracheal-bronchial tree are numerically analyzed using the computational fluid dynamics (CFD) package CFX. The model used is a four-generation airway that is geometrically similar to Weibel's symmetrical model. In the present analysis, two different models (in-plane and off-plane) are examined for a wide range of Reynolds numbers that correspond to human breathing conditions. The findings indicate that the secondary flow patterns generated become more significant as the flow passes from the trachea to the fourth-generation airway. Moreover, comparison between in-plane and off-plane models shows that the skewed velocity profiles and secondary flows for the in-plane model are more prominent than those for the off-plane one. In general, the model developed in this study is capable of providing an overall insight into the effect of fluid flow in multiple generations of the human upper respiratory airways.

scholarly journals Hydrodynamic particle focusing enhanced by femtosecond laser deep grooving at low Reynolds numbers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tianlong Zhang ◽  
Misuzu Namoto ◽  
Kazunori Okano ◽  
Eri Akita ◽  
Norihiro Teranishi ◽  
...  
Keyword(s):  
Flow Rate ◽  
Reynolds Numbers ◽  
Secondary Flows ◽  
Low Flow ◽  
Live Cells ◽  
Low Reynolds Numbers ◽  
Particle Focusing ◽  
Human T Cell ◽  
Fs Laser ◽  
Low Reynolds

AbstractMicrofluidic focusing of particles (both synthetic and biological), which enables precise control over the positions of particles in a tightly focused stream, is a prerequisite step for the downstream processing, such as detection, trapping and separation. In this study, we propose a novel hydrodynamic focusing method by taking advantage of open v-shaped microstructures on a glass substrate engraved by femtosecond pulse (fs) laser. The fs laser engraved microstructures were capable of focusing polystyrene particles and live cells in rectangular microchannels at relatively low Reynolds numbers (Re). Numerical simulations were performed to explain the mechanisms of particle focusing and experiments were carried out to investigate the effects of groove depth, groove number and flow rate on the performance of the groove-embedded microchannel for particle focusing. We found out that 10-µm polystyrene particles are directed toward the channel center under the effects of the groove-induced secondary flows in low-Re flows, e.g. Re < 1. Moreover, we achieved continuous focusing of live cells with different sizes ranging from 10 to 15 µm, i.e. human T-cell lymphoma Jurkat cells, rat adrenal pheochromocytoma PC12 cells and dog kidney MDCK cells. The glass grooves fabricated by fs laser are expected to be integrated with on-chip detection components, such as contact imaging and fluorescence lifetime-resolved imaging, for various biological and biomedical applications, where particle focusing at a relatively low flow rate is desirable.

Turbulent three-dimensional dielectric electrohydrodynamic convection between two plates

2012 ◽  
Vol 696 ◽  
pp. 228-262 ◽  
Author(s):  
A. Kourmatzis ◽  
J. S. Shrimpton
Keyword(s):  
Spatial Data ◽  
Three Dimensional ◽  
Reynolds Numbers ◽  
Two Dimensions ◽  
Three Dimensions ◽  
Energy Budgets ◽  
Turbulent Regime ◽  
Scalar Flux ◽  
Wide Range ◽  
Scalar Variance

AbstractThe fundamental mechanisms responsible for the creation of electrohydrodynamically driven roll structures in free electroconvection between two plates are analysed with reference to traditional Rayleigh–Bénard convection (RBC). Previously available knowledge limited to two dimensions is extended to three-dimensions, and a wide range of electric Reynolds numbers is analysed, extending into a fully inherently three-dimensional turbulent regime. Results reveal that structures appearing in three-dimensional electrohydrodynamics (EHD) are similar to those observed for RBC, and while two-dimensional EHD results bear some similarities with the three-dimensional results there are distinct differences. Analysis of two-point correlations and integral length scales show that full three-dimensional electroconvection is more chaotic than in two dimensions and this is also noted by qualitatively observing the roll structures that arise for both low (${\mathit{Re}}_{E} = 1$) and high electric Reynolds numbers (up to ${\mathit{Re}}_{E} = 120$). Furthermore, calculations of mean profiles and second-order moments along with energy budgets and spectra have examined the validity of neglecting the fluctuating electric field ${ E}_{i}^{\ensuremath{\prime} } $ in the Reynolds-averaged EHD equations and provide insight into the generation and transport mechanisms of turbulent EHD. Spectral and spatial data clearly indicate how fluctuating energy is transferred from electrical to hydrodynamic forms, on moving through the domain away from the charging electrode. It is shown that ${ E}_{i}^{\ensuremath{\prime} } $ is not negligible close to the walls and terms acting as sources and sinks in the turbulent kinetic energy, turbulent scalar flux and turbulent scalar variance equations are examined. Profiles of hydrodynamic terms in the budgets resemble those in the literature for RBC; however there are terms specific to EHD that are significant, indicating that the transfer of energy in EHD is also attributed to further electrodynamic terms and a strong coupling exists between the charge flux and variance, due to the ionic drift term.

scholarly journals Steady flow separation patterns in a 45 degree junction

2000 ◽  
Vol 411 ◽  
pp. 1-38 ◽  
Author(s):  
C. ROSS ETHIER ◽  
SUJATA PRAKASH ◽  
DAVID A. STEINMAN ◽  
RICHARD L. LEASK ◽  
GREGORY G. COUCH ◽  
...  
Keyword(s):  
Flow Separation ◽  
Stokes Equations ◽  
Bypass Graft ◽  
Three Dimensional ◽  
Symmetry Plane ◽  
Axial Flow ◽  
Reynolds Numbers ◽  
Secondary Flows ◽  
Measured Velocity ◽  
Curved Tube

Numerical and experimental techniques were used to study the physics of flow separation for steady internal flow in a 45° junction geometry, such as that observed between two pipes or between the downstream end of a bypass graft and an artery. The three-dimensional Navier–Stokes equations were solved using a validated finite element code, and complementary experiments were performed using the photochromic dye tracer technique. Inlet Reynolds numbers in the range 250 to 1650 were considered. An adaptive mesh refinement approach was adopted to ensure grid-independent solutions. Good agreement was observed between the numerical results and the experimentally measured velocity fields; however, the wall shear stress agreement was less satisfactory. Just distal to the ‘toe’ of the junction, axial flow separation was observed for all Reynolds numbers greater than 250. Further downstream (approximately 1.3 diameters from the toe), the axial flow again separated for Re [ges ] 450. The location and structure of axial flow separation in this geometry is controlled by secondary flows, which at sufficiently high Re create free stagnation points on the model symmetry plane. In fact, separation in this flow is best explained by a secondary flow boundary layer collision model, analogous to that proposed for flow in the entry region of a curved tube. Novel features of this flow include axial flow separation at modest Re (as compared to flow in a curved tube, where separation occurs only at much higher Re), and the existence and interaction of two distinct three-dimensional separation zones.

The Effect of Pulsed Blowing on the Boundary Layer of a Highly Loaded Low Pressure Turbine Blade

2013 ◽  
Author(s):  
Marion Mack ◽  
Roland Brachmanski ◽  
Reinhard Niehuis
Keyword(s):  
Boundary Layer ◽  
Reynolds Number ◽  
Mach Number ◽  
Suction Side ◽  
Reynolds Numbers ◽  
Low Pressure ◽  
Trailing Edge ◽  
Low Pressure Turbine ◽  
Wide Range ◽  
Highly Loaded

The performance of the low pressure turbine (LPT) can vary appreciably, because this component operates under a wide range of Reynolds numbers. At higher Reynolds numbers, mid and aft loaded profiles have the advantage that transition of suction side boundary layer happens further downstream than at front loaded profiles, resulting in lower profile loss. At lower Reynolds numbers, aft loading of the blade can mean that if a suction side separation exists, it may remain open up to the trailing edge. This is especially the case when blade lift is increased via increased pitch to chord ratio. There is a trend in research towards exploring the effect of coupling boundary layer control with highly loaded turbine blades, in order to maximize performance over the full relevant Reynolds number range. In an earlier work, pulsed blowing with fluidic oscillators was shown to be effective in reducing the extent of the separated flow region and to significantly decrease the profile losses caused by separation over a wide range of Reynolds numbers. These experiments were carried out in the High-Speed Cascade Wind Tunnel of the German Federal Armed Forces University Munich, Germany, which allows to capture the effects of pulsed blowing at engine relevant conditions. The assumed control mechanism was the triggering of boundary layer transition by excitation of the Tollmien-Schlichting waves. The current work aims to gain further insight into the effects of pulsed blowing. It investigates the effect of a highly efficient configuration of pulsed blowing at a frequency of 9.5 kHz on the boundary layer at a Reynolds number of 70000 and exit Mach number of 0.6. The boundary layer profiles were measured at five positions between peak Mach number and the trailing edge with hot wire anemometry and pneumatic probes. Experiments were conducted with and without actuation under steady as well as periodically unsteady inflow conditions. The results show the development of the boundary layer and its interaction with incoming wakes. It is shown that pulsed blowing accelerates transition over the separation bubble and drastically reduces the boundary layer thickness.

Predictions of the Effect of Roughness on Heat Transfer From Turbine Airfoils

1998 ◽  
Author(s):  
Anil K. Tolpadi ◽  
Michael E. Crawford
Keyword(s):  
Heat Transfer ◽  
Side Surface ◽  
Suction Side ◽  
Reynolds Numbers ◽  
Surface Finishing ◽  
Transfer Coefficients ◽  
Average Roughness ◽  
Wide Range ◽  
Turbine Airfoils ◽  
Good Agreement

The heat transfer and aerodynamic performance of turbine airfoils are greatly influenced by the gas side surface finish. In order to operate at higher efficiencies and to have reduced cooling requirements, airfoil designs require better surface finishing processes to create smoother surfaces. In this paper, three different cast airfoils were analyzed: the first airfoil was grit blasted and codep coated, the second airfoil was tumbled and aluminide coated, and the third airfoil was polished further. Each of these airfoils had different levels of roughness. The TEXSTAN boundary layer code was used to make predictions of the heat transfer along both the pressure and suction sides of all three airfoils. These predictions have been compared to corresponding heat transfer data reported earlier by Abuaf et al. (1997). The data were obtained over a wide range of Reynolds numbers simulating typical aircraft engine conditions. A three-parameter full-cone based roughness model was implemented in TEXSTAN and used for the predictions. The three parameters were the centerline average roughness, the cone height and the cone-to-cone pitch. The heat transfer coefficient predictions indicated good agreement with the data over most Reynolds numbers and for all airfoils-both pressure and suction sides. The transition location on the pressure side was well predicted for all airfoils; on the suction side, transition was well predicted at the higher Reynolds numbers but was computed to be somewhat early at the lower Reynolds numbers. Also, at lower Reynolds numbers, the heat transfer coefficients were not in very good agreement with the data on the suction side.

CFD Simulation of Fluid Flow Through Porous Media: Application to Decomposed Gases Flow Through Foam Pattern in Lost Foam Casting

Materials ◽  
2003 ◽  
Author(s):  
Sayavur I. Bakhtiyarov ◽  
Ruel A. Overfelt
Keyword(s):  
Cfd Simulation ◽  
Reynolds Numbers ◽  
Phase Flow ◽  
Flow Through Porous Media ◽  
Foam Material ◽  
Element Analysis ◽  
Wide Range ◽  
Flow Through ◽  
Foam Pattern ◽  
Lost Foam

Numerical simulation of decomposed gases through foam pattern was conducted using finite element analysis. A new kinetic model is proposed for gaseos phase flow between molten metal and foam material. The computations were performed for a wide range of Reynolds numbers. The results of the simulations are compared with the experiemental data obtained in this study.

Calculation of the primary trajectories of seeds and other particles in strong winds

1983 ◽  
Vol 219 (1215) ◽  
pp. 217-217
Keyword(s):  
Equations Of Motion ◽  
Aerodynamic Resistance ◽  
Reynolds Numbers ◽  
Spherical Particles ◽  
Spores And Pollen ◽  
General Terms ◽  
Wide Range ◽  
Strong Winds ◽  
Dispersal Distances ◽  
Enormous Number

The movement of variously dense spherical particles representing a variety of seeds, fruits, spores and pollen, and released from rest into arbitrary winds and a gravitational field is discussed in general terms that account in detail for changes in the quasi-static aerodynamic resistance to motion experienced by such particles during aerial flight. A hybrid analytical-empirical law is established which describes this resistance fairly accurately for particle Reynolds numbers in the range 0—60 000 and that allows for the numerical integration of the equations of motion so as to cover a very wide range of flight conditions. This makes possible the provision of a set of four-parameter universal range tables from which the dispersal distances for an enormous number of practical cases may be estimated. One particular case of particle movement in a region of pseudo-thermal convection is also discussed and this shows how a marked degree of deposition concentration may be induced in some circumstances by such a flow. Botanists and ecologists concerned with seed and particle dispersal in the environment may find the universal range tables of particular interest and use. This is because the tables obviate the need for the integration of the equations of motion when dealing with individual cases and permit an estimation of range purely on the basis of the specified quantities of particle size, density and altitude of release, atmospheric wind speed, density and viscosity, and the acceleration due to gravity.

The viscous secondary flow ahead of an infinite cylinder in a uniform parallel shear flow

1960 ◽  
Vol 7 (1) ◽  
pp. 145-155 ◽  
Author(s):  
Alar Toomre
Keyword(s):  
Shear Flow ◽  
Lateral Displacement ◽  
Reynolds Numbers ◽  
Infinite Cylinder ◽  
List Type ◽  
Displacement Effect ◽  
Simple Method ◽  
Viscous Shear ◽  
Parallel Shear Flow ◽  
Viscous Shear Flow

A simple method is presented in this paper for calculating the secondary velocities, andthe lateral displacement of total pressure surfaces (i.e. the ‘displacement effect’) in the plane of symmetry ahead of an infinitely long cylinder situated normal to a steady, incompressible, slightly viscous shear flow; the cylinder is also perpendicular to the vorticity, which is assumed uniform but small. The method is based on lateral gradients of pressure, these being calculated from the primary flow alone. Profiles of the secondary velocities are obtained at several Reynolds numbers ahead of two specific cylindrical shapes: a circular cylinder, and a flat plate normal to the flow. The displacement effect is derived and, rathe surprisingly, is found to be virtually independent of the Reynolds number.

Experimental Investigation of Subsonic Turbulent Flow Over Single and Double Backward Facing Steps

1962 ◽  
Vol 84 (3) ◽  
pp. 317-325 ◽  
Author(s):  
D. E. Abbott ◽  
S. J. Kline
Keyword(s):  
Experimental Investigation ◽  
Velocity Profile ◽  
Flow Pattern ◽  
Reynolds Numbers ◽  
Area Ratio ◽  
Single Step ◽  
Reattachment Length ◽  
Double Step ◽  
Wide Range ◽  
Geometric Variables

Results are presented for flow patterns over backward facing steps covering a wide range of geometric variables. Velocity profile measurements are given for both single and double steps. The stall region is shown to consist of a complex pattern involving three distinct regions. The double step contains an assymmetry for large expansions, but approaches the single-step configuration with symmetric stall regions for small values of area ratio. No effect on flow pattern or reattachment length is found for a wide range of Reynolds numbers and turbulence intensities, provided the flow is fully turbulent before the step.

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