scholarly journals Distinct Functional Traits of Lactobacilli from Women with Asymptomatic Bacterial Vaginosis and Normal Microbiota

2020 ◽  
Vol 8 (12) ◽  
pp. 1949
Author(s):  
Rinku Pramanick ◽  
Clara Aranha
Keyword(s):  
Lactic Acid ◽  
Bacterial Vaginosis ◽  
Functional Properties ◽  
Reproductive Age ◽  
E Coli ◽  
Vaginal Lactobacilli ◽  
Normal Microbiota ◽  
High Incidence ◽  
Culture Supernatants ◽  
Asymptomatic Women

Asymptomatic bacterial vaginosis (BV) in reproductive-age women has serious obstetric and gynecological consequences. Despite its high incidence, the behavior of vaginal lactobacilli in asymptomatic BV is unknown. We analyzed the functional properties of previously isolated vaginal lactobacilli from asymptomatic women with normal, intermediate, and BV microbiota. Lactic acid and antimicrobial activity against seven urogenital pathogens were evaluated from lactobacilli cell-free culture supernatants (CFCs) (n = 207) after 48 h incubation in MRS. Lactobacilli isolates were used to evaluate H2O2, autoaggregation and coaggregation with C. albicans. Lactobacilli from normal microbiota produced more d-lactate than lactobacilli from intermediate and asymptomatic BV (p = 0.007). L. plantarum, L. fermentum and L. reuteri produced greater d-lactate whereas L. rhamnosus, L. crispatus, L. johnsonii were greater producers of l-lactate. Interspecies positive correlation was observed in the lactic acid contents of CFCs. Distribution of H2O2-producing lactobacilli did not vary significantly among the groups. When lactic acid isomers were considered, species from intermediate and BV microbiota clustered together with each other and distinctly from species of normal microbiota. Broad-spectrum antagonism (≥90% inhibition) against E. coli, C. albicans, S. aureus, P. aeruginosa, G. vaginalis, N. gonorrhoeae, S. agalactiae were displayed by 46.86% (97) of isolates. Our study highlights the differential functional properties of vaginal lactobacilli from women with normal microbiota and asymptomatic BV.

scholarly journals Cross sectional analysis of vaginal Lactobacillus in asymptomatic women of reproductive age in Mumbai, India

2018 ◽  
Vol 12 (12) ◽  
pp. 1096-1104 ◽  
Author(s):  
Rinku Pramanick ◽  
Shraddha Parab ◽  
Niranjan Mayadeo ◽  
Himangi Warke ◽  
Clara Aranha
Keyword(s):  
Lactic Acid ◽  
Race And Ethnicity ◽  
Premenopausal Women ◽  
Reproductive Age ◽  
Lactobacillus Species ◽  
H2o2 Production ◽  
Cross Sectional ◽  
Vaginal Microflora ◽  
Vaginal Lactobacilli ◽  
Normal Women

Introduction: Lactobacillus dominated vaginal microenvironment is associated with lower risk of genital infections. Numerous studies have reported geographic and ethnic variations in vaginal microbiome structure between healthy individuals from different race and ethnicity. India has a great diversity, so it is intriguing to find out if such divergences exist in vaginal lactobacilli. The present study aimed to investigate predominant Lactobacillus species in vaginas of healthy Indian women and screen isolates for lactic acid and H2O2 production. Methodology: 203 premenopausal women asymptomatic for any vaginal complaints were recruited. The lactobacilli isolates on MRS agar were identified by Multiplex-PCR and 16sRNA gene sequencing. RAPD was used to differentiate strains of same species. H2O2 and lactic acid was evaluated on TMB-HRP MRS agar and BCP-MRS agar respectively. Results: Lactobacilli were recovered from 107/109 (98.2%) women with normal microflora. L. iners 64.7% (68), L. crispatus 26.7% (28), L. reuteri 21.9% (23), L. jensenii 16.2% (17) and L. gasseri 15.2% (16) were the most frequently occurring vaginal lactobacilli in normal women. The vaginal microflora was dominated by either by a single (80%, n = 84) or a combination (20%, n = 21) of Lactobacillus species. Though most frequently identified, L. iners, coexisted only with other Lactobacillus species. All isolates were acid producers but H2O2 was produced by 94.2% isolates. Conclusions: Our study reports prevalent vaginal lactobacilli which could be explored as probiotics. Presence of heterogeneous Lactobacillus population highlights the cumulative effects of different lactobacilli maintaining vaginal health. Contrasting observations about L. iners reiterates its puzzling role in vaginal immunity, advocating further research.

scholarly journals Cationic amphiphiles against Gardnerella vaginalis resistant strains and bacterial vaginosis-associated pathogens

2019 ◽  
Vol 77 (8) ◽  
Author(s):  
Richard M Weeks ◽  
Alysha Moretti ◽  
Shuang Song ◽  
Kathryn E Uhrich ◽  
Andrey V Karlyshev ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Reproductive Age ◽  
Gardnerella Vaginalis ◽  
Cationic Amphiphiles ◽  
Commensal Microbiota ◽  
Head Groups ◽  
Vaginal Lactobacilli ◽  
Critical Issues ◽  
Resistant Strains ◽  
Infection Recurrence

ABSTRACT Antibiotic resistance and infection recurrence are critical issues in treating bacterial vaginosis, the most common vaginal disorder in women of reproductive age. Novel alternatives to traditional antibiotics, such as peptidomimetics, have the potential to address this challenge. Previously, two series of cationic amphiphiles (CAms) were developed with both hydrophilic head groups and non-polar domains, giving them the ability to self-assemble into supramolecular nanostructures with membrane-lytic properties. Those CAms were shown to be effective against biofilms of Gardnerella vaginalis while preserving the commensal microbiota. Two new series of CAms were designed with varying levels of flexibility between the hydrophilic head groups and the hydrophobic domains. Activities against the vaginal pathogen G. vaginalis ranged from 1.3 to 18.5 µM, while the tested vaginal lactobacilli were significantly more tolerant of CAms, with minimal inhibitory concentration values as high as 208 µM. Minimal biofilm bactericidal concentrations of the tested CAms ranged from 21.47 to <388.3 µM, and were lowest against resistant forms of G. vaginalis, while Lactobacillus biofilms were tolerant of concentrations ≥687 µM. Safety aspects of the CAms were also investigated, and they were found to be safe for use against vaginal ectocervical tissue.

scholarly journals Technological Characterization of Lactic Acid Bacteria Isolated from Different Sheep’s Milk

2021 ◽  
Author(s):  
L. Ketrouci ◽  
F. Dalache ◽  
D. Benabdelmoumene ◽  
A.A. Dahou ◽  
A. Homrani
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lipolytic Activity ◽  
Tween 80 ◽  
Antagonist Activity ◽  
E Coli ◽  
Acidification Capacity ◽  
Culture Supernatants ◽  
Exopolysaccharides Production

Background: Technological characterization of lactic acid bacteria isolated from sheep’s milk collected in 3 regions in northwestern Algeria.Methods: During the period from 2018 to 2019, fifty strains of Lactic acid bacteria isolated from samples sheep’s milk were evaluated for several technologically-relevant properties: diacetyl and exopolysaccharides production, acidification, proteolytic and lipolytic activity and their antagonist activity against Escherichia coli and Pseudomonas aeruginosa. Result: The results indicate that among all the isolates only 20% were distinguished by their production of EPS mainly the genus Leuconostoc. Diacetyl production was observed in 71% of Lactobacillus, 60% of Enterococcus and 25% in Leuconostoc. 94% isolates showed moderate proteolytic activity. 56% and 60% of the strains degraded tween 80 and olive oil respectively for lipolytic activity. Inhibition activity by the cultures LAB was about 82% and 78% against E. coli and P. aeruginosa respectively. No culture supernatants inhibit P. aeruginosa, however 18% of the Enterococcus trains inhibit E. coli. BME1.A2 and BME2.D4 showed their highest acidification capacity developing a very large quantity of lactic acid after 24 h of incubation, i.e., 7.6 and 8.4 g lactic acid/L respectively.

Advantages of personalized therapies in the treatment of bacterial vaginosis in women reproductive age

2016 ◽  
pp. 131-136
Author(s):  
V.O. Tovstanovskyy ◽  
◽  
A.E. Alatorskih ◽  
Faranak Parsal ◽  
◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Reproductive Age

scholarly journals PREVALENCE AND CORRELATES OF BACTERIAL VAGINOSIS AMONG YOUNG WOMEN OF REPRODUCTIVE AGE IN MYSORE, INDIA

2008 ◽  
Vol 26 (2) ◽  
pp. 132-137
Author(s):  
P Madhivanan ◽  
K Krupp ◽  
V Chandrasekaran ◽  
C Karat ◽  
A Arun ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Young Women ◽  
Reproductive Age ◽  
Women Of Reproductive Age

Techno-functional properties of HoPS from lactic acid bacteria of different origins as potential food additives

2021 ◽  
Vol 356 ◽  
pp. 129627
Author(s):  
René Emanuel Lobo ◽  
Theo Figueroa ◽  
Diego Navarro ◽  
María Inés Gómez ◽  
Graciela Font de Valdez ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Functional Properties ◽  
Food Additives ◽  
Different Origins ◽  
Potential Food

Acid resistance of E. coli O157:H7 and O26:H11 exposure to lactic acid revealed by transcriptomic analysis

LWT ◽  
2021 ◽  
Vol 136 ◽  
pp. 110352
Author(s):  
Lanlin Yu ◽  
Su Zhang ◽  
Yuanyuan Xu ◽  
Xiaoyu Mi ◽  
Tong Xing ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Resistance ◽  
Transcriptomic Analysis ◽  
E Coli

In Vitro Inhibition of the Growth of Salmonella typhimurium and Escherichia coli 0157:H7 by Bacteria Isolated from the Cecal Contents of Adult Chickens

1991 ◽  
Vol 54 (7) ◽  
pp. 496-501 ◽  
Author(s):  
ARTHUR HINTON ◽  
GEORGE E. SPATES ◽  
DONALD E. CORRIER ◽  
MICHAEL E. HUME ◽  
JOHN R. DELOACH ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Salmonella Typhimurium ◽  
Mixed Cultures ◽  
Enterococcus Durans ◽  
E Coli ◽  
Pure Cultures ◽  
In Vitro Inhibition ◽  
Lactic Acids

A Veillonella species and Enterococcus durans were isolated from the cecal contents of adult broilers. Mixed cultures of Veillonella and E. durans inhibited the growth of Salmonella typhimurium and Escherichia coli 0157:H7 on media containing 2.5% lactose (w/v). The growth of S. typhimurium or E. coli 0157:H7 was not inhibited by mixed cultures containing Veillonella and E. durans on media containing only 0.25% lactose or by pure cultures of Veillonella or E. durans on media containing either 0.25% or 2.5% lactose. The mixed cultures of Veillonella and E. durans produced significantly (P<0.05) more acetic, propionic, and lactic acids in media containing 2.5% lactose than in media containing 0.25% lactose. The inhibition of the enteropathogens was related to the production of lactic acid from lactose by the E. durans and the production of acetic and propionic acids from lactic acid by the Veillonella.

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