scholarly journals Cross sectional analysis of vaginal Lactobacillus in asymptomatic women of reproductive age in Mumbai, India

2018 ◽  
Vol 12 (12) ◽  
pp. 1096-1104 ◽  
Author(s):  
Rinku Pramanick ◽  
Shraddha Parab ◽  
Niranjan Mayadeo ◽  
Himangi Warke ◽  
Clara Aranha
Keyword(s):  
Lactic Acid ◽  
Race And Ethnicity ◽  
Premenopausal Women ◽  
Reproductive Age ◽  
Lactobacillus Species ◽  
H2o2 Production ◽  
Cross Sectional ◽  
Vaginal Microflora ◽  
Vaginal Lactobacilli ◽  
Normal Women

Introduction: Lactobacillus dominated vaginal microenvironment is associated with lower risk of genital infections. Numerous studies have reported geographic and ethnic variations in vaginal microbiome structure between healthy individuals from different race and ethnicity. India has a great diversity, so it is intriguing to find out if such divergences exist in vaginal lactobacilli. The present study aimed to investigate predominant Lactobacillus species in vaginas of healthy Indian women and screen isolates for lactic acid and H2O2 production. Methodology: 203 premenopausal women asymptomatic for any vaginal complaints were recruited. The lactobacilli isolates on MRS agar were identified by Multiplex-PCR and 16sRNA gene sequencing. RAPD was used to differentiate strains of same species. H2O2 and lactic acid was evaluated on TMB-HRP MRS agar and BCP-MRS agar respectively. Results: Lactobacilli were recovered from 107/109 (98.2%) women with normal microflora. L. iners 64.7% (68), L. crispatus 26.7% (28), L. reuteri 21.9% (23), L. jensenii 16.2% (17) and L. gasseri 15.2% (16) were the most frequently occurring vaginal lactobacilli in normal women. The vaginal microflora was dominated by either by a single (80%, n = 84) or a combination (20%, n = 21) of Lactobacillus species. Though most frequently identified, L. iners, coexisted only with other Lactobacillus species. All isolates were acid producers but H2O2 was produced by 94.2% isolates. Conclusions: Our study reports prevalent vaginal lactobacilli which could be explored as probiotics. Presence of heterogeneous Lactobacillus population highlights the cumulative effects of different lactobacilli maintaining vaginal health. Contrasting observations about L. iners reiterates its puzzling role in vaginal immunity, advocating further research.

Anti-Müllerian Hormone Concentrations Are Inversely Associated With Subclinical Atherosclerosis in Premenopausal Women

Angiology ◽  
2020 ◽  
Vol 71 (6) ◽  
pp. 552-558 ◽  
Author(s):  
Irene Lambrinoudaki ◽  
Stefanos Stergiotis ◽  
Panagiota Chatzivasileiou ◽  
Areti Augoulea ◽  
Panagiotis Anagnostis ◽  
...  
Keyword(s):  
Risk Factors ◽  
Ovarian Reserve ◽  
Augmentation Index ◽  
Subclinical Atherosclerosis ◽  
Premenopausal Women ◽  
Reproductive Age ◽  
Inverse Association ◽  
Cross Sectional ◽  
Carotid Imt ◽  
Negatively Associated

Anti-Müllerian hormone (AMH), which is secreted by granulosa cells of late preantral and small antral follicles, is a marker of ovarian reserve. The association of ovarian reserve with subclinical atherosclerosis in women of reproductive age is currently unknown. We primary investigated whether AMH levels are associated with markers of subclinical atherosclerosis in healthy, normally menstruating women. In this cross-sectional study, vascular structure and function were assessed by measurement of carotid and femoral intima–media thickness (IMT), flow-mediated dilation, carotid–femoral pulse wave velocity and augmentation index. Lipid profile and serum AMH concentrations were also measured. Seventy premenopausal women, aged 32.7 ± 6.5 years, were included. Mean AMH levels were lower in smokers than in non-smokers and negatively associated with total cholesterol (TC) levels. An inverse association between mean AMH concentrations and femoral and carotid IMT in all segments was observed. No correlation with other markers of subclinical atherosclerosis or established cardiovascular (CV) risk factors was found. After multivariable adjustment, the association between AMH concentrations and combined carotid IMT or carotid bulb IMT remained significant. In conclusion, in healthy, normally ovulating women, AMH concentrations are negatively associated with subclinical atherosclerosis indices and TC levels, independently of established CV risk factors.

Bone Mineral Content of the Lumbar Spine in Normal and Osteoporotic Women: Cross-Sectional and Longitudinal Studies

1982 ◽  
Vol 62 (3) ◽  
pp. 329-336 ◽  
Author(s):  
B. Krølner ◽  
S. Pors Nielsen
Keyword(s):  
Bone Loss ◽  
Femoral Neck ◽  
Bone Mineral Content ◽  
Bone Mineral ◽  
Mineral Content ◽  
Premenopausal Women ◽  
Femoral Neck Fractures ◽  
Cross Sectional ◽  
Compression Fractures ◽  
Normal Women

1. Bone mineral content of the second, third and fourth lumbar vertebrae was determined in normal women and women with clinical osteoporosis by using dual-photon (153Gd) absorptiometry. 2. A cross-sectional study of 70 normal women (aged 19–88 years) showed a bone loss of 44% from the age of around 34 years throughout life. 3. Longitudinal data from 59 normal women confirmed that the vertebral bone loss started before the menopause. An accelerated bone loss amounting to nearly 6% per year was seen immediately after the menopause. The bone loss of older women was slower. 4. Mean lumbar bone mineral content of 36 women (aged 48–93 years) with recent fractures of their femoral neck after minor trauma equalled that of age-matched normal women. Lumbar bone mineral content of the women with intratrochanteric femoral neck fractures was lower than that of the women with medial femoral neck fractures. 5. Mean lumbar bone mineral content of 72 women (aged 58–89 years) with primary osteoporosis was 41% lower than that of normal premenopausal women and 18% lower than that of age-matched controls. A weak inverse relationship between lumbar bone mineral content and the number of compression fractures was found. A weak inverse relationship between lumbar bone mineral content and the number of compression fractures was found. 6. Women with lumbar bone mineral content values below the 95% confidence limits for normal premenopausal women are at risk of future vertebral compression fractures, the fracture risk being inversely related to lumbar bone mineral content.

scholarly journals Current views on vaginal lactobacilli in women of reproductive age

2016 ◽  
Vol 65 (4) ◽  
pp. 34-43 ◽  
Author(s):  
Olga V. Budilovskaya
Keyword(s):  
Species Diversity ◽  
Current Data ◽  
Vaginal Microbiota ◽  
Controversial Issue ◽  
Reproductive Age ◽  
Lactobacillus Species ◽  
Women Of Reproductive Age ◽  
Vaginal Lactobacilli ◽  
Antimicrobial Protection

The paper presents the current data on the species diversity of lactobacilli in women of reproductive age and their role in maintaining the normal vaginal microbiocenosis. Attention was paid to genotypic features of some lactobacillus species and the mechanisms providing antimicrobial protection of the vaginal biotope. The controversial issue of the ability of Lactobacillus iners to ensure stability of the normal vaginal microbiota.

scholarly journals Species diversity of vaginal lactobacilli in norm and in dysbiotic states

2017 ◽  
Vol 66 (2) ◽  
pp. 24-32
Author(s):  
Olga V Budilovskaya ◽  
Elena V Shipitsyna ◽  
Ekaterina N Gerasimova ◽  
Margarita M Safronova ◽  
Alevtina M Savicheva
Keyword(s):  
Species Diversity ◽  
Reproductive Age ◽  
Quantitative Composition ◽  
Opportunistic Pathogens ◽  
Quantitative Real Time Pcr ◽  
Women Of Reproductive Age ◽  
Healthy Women ◽  
Vaginal Microflora ◽  
Vaginal Lactobacilli ◽  
Lactobacillus Jensenii

Introduction. In healthy women of reproductive age, the vaginal microflora is represented mainly by lactobacilli. They provide a barrier function, preventing the propagation of opportunistic pathogens and the colonization of the vagina by pathogenic microorganisms. It is shown that the lactobacillary microflora of the vagina is very diverse, but not all species of lactobacilli can provide reliable protection of the female organism. Objective: to characterize the species and quantitative composition of vaginal lactobacilli of women of reproductive age in norm and in dysbiosis. Material and methods. The study involved 123 patients from polyclinic departments of the D.O. Ott Reasearch Institute of Obstetrics, Gynecology and Reproductology. For analysis of clinical material (vaginal discharge) for lactobacilli and other microorganisms, quantitative real-time PCR was used. Results. The most common types of lactobacilli are Lactobacillus jensenii, L. Iners, L. crispatus, L. vaginalis and L. gasseri. Species diversity of lactobacilli (detection of ≥2 species) is observed much more often in women with physiological microbiocenosis than in women with vaginal dysbiosis. Our study confirms that L. crispatus is the dominant species of the vaginal biotope of healthy women, while in dysbiosis the species L. gasseri and L. Iners are most often identified.

scholarly journals Distinct Functional Traits of Lactobacilli from Women with Asymptomatic Bacterial Vaginosis and Normal Microbiota

2020 ◽  
Vol 8 (12) ◽  
pp. 1949
Author(s):  
Rinku Pramanick ◽  
Clara Aranha
Keyword(s):  
Lactic Acid ◽  
Bacterial Vaginosis ◽  
Functional Properties ◽  
Reproductive Age ◽  
E Coli ◽  
Vaginal Lactobacilli ◽  
Normal Microbiota ◽  
High Incidence ◽  
Culture Supernatants ◽  
Asymptomatic Women

Asymptomatic bacterial vaginosis (BV) in reproductive-age women has serious obstetric and gynecological consequences. Despite its high incidence, the behavior of vaginal lactobacilli in asymptomatic BV is unknown. We analyzed the functional properties of previously isolated vaginal lactobacilli from asymptomatic women with normal, intermediate, and BV microbiota. Lactic acid and antimicrobial activity against seven urogenital pathogens were evaluated from lactobacilli cell-free culture supernatants (CFCs) (n = 207) after 48 h incubation in MRS. Lactobacilli isolates were used to evaluate H2O2, autoaggregation and coaggregation with C. albicans. Lactobacilli from normal microbiota produced more d-lactate than lactobacilli from intermediate and asymptomatic BV (p = 0.007). L. plantarum, L. fermentum and L. reuteri produced greater d-lactate whereas L. rhamnosus, L. crispatus, L. johnsonii were greater producers of l-lactate. Interspecies positive correlation was observed in the lactic acid contents of CFCs. Distribution of H2O2-producing lactobacilli did not vary significantly among the groups. When lactic acid isomers were considered, species from intermediate and BV microbiota clustered together with each other and distinctly from species of normal microbiota. Broad-spectrum antagonism (≥90% inhibition) against E. coli, C. albicans, S. aureus, P. aeruginosa, G. vaginalis, N. gonorrhoeae, S. agalactiae were displayed by 46.86% (97) of isolates. Our study highlights the differential functional properties of vaginal lactobacilli from women with normal microbiota and asymptomatic BV.

Homocysteine Levels Show Significant Differences among Mediterranean Dietary Quality Index Variables Compared to Folate and Vitamin B12 Status in Women

2015 ◽  
Vol 85 (3-4) ◽  
pp. 202-210 ◽  
Author(s):  
Ivona Višekruna ◽  
Ivana Rumbak ◽  
Ivana Rumora Samarin ◽  
Irena Keser ◽  
Jasmina Ranilović
Keyword(s):  
Mediterranean Diet ◽  
Diet Quality ◽  
Quality Index ◽  
Fruits And Vegetables ◽  
Reproductive Age ◽  
Dietary Quality ◽  
Vitamin B ◽  
Cross Sectional ◽  
Poor Diet ◽  
The Mediterranean

Abstract. Results of epidemiologic studies and clinical trials have shown that subjects following the Mediterranean diet had lower inflammatory markers such as homocysteine (Hcy). Therefore, the aim of this cross-sectional study was to assess female diet quality with the Mediterranean diet quality index (MDQI) and to determine the correlation between MDQI, homocysteine, folate and vitamin B12 levels in the blood. The study participants were 237 apparently healthy women (96 of reproductive age and 141 postmenopausal) between 25 and 93 years. For each participant, 24-hour dietary recalls for 3 days were collected, MDQI was calculated, and plasma Hcy, serum and erythrocyte folate and vitamin B12 levels were analysed. Total MDQI ranged from 8 to 10 points, which represented a medium-poor diet for the subjects. The strength of correlation using biomarkers, regardless of group type, age, gender and other measured parameters, was ranked from best (0.11) to worst (0.52) for olive oil, fish, fruits and vegetables, grains, and meat, in this order. Hcy levels showed the best response among all markers across all groups and food types. Our study shows significant differences between variables of the MDQI and Hcy levels compared to levels of folate and vitamin B12 in participants with medium-poor diet quality, as evaluated according to MDQI scores.

Factor Related With the Anemia Incidence Of Adolescent Girl in Private High School Santa Lusia Medan, 2019

2020 ◽  
Vol 4 (2) ◽  
pp. 33
Author(s):  
Risda Mariana Manik ◽  
Hetty Gustina Simamora
Keyword(s):  
High School ◽  
Adolescent Girl ◽  
Sampling Technique ◽  
Consumption Patterns ◽  
Reproductive Age ◽  
Digital Data ◽  
Deficiency Anemia ◽  
Consumption Pattern ◽  
Cross Sectional ◽  
Private High School

According to data Basic Healt Research (Riskesdas) in 2016, as many as 22,7% women of reproductive age more than 15 years indicate anemia. Anemia that often occurs is iron deficiency anemia, the incidence reaches 50% of the total anemia. There is a significant relationship between nutritional status and incidence of anemia in adolescent.This study was an observational analytic with a cross sectional approach. This study was conducted in private high school Santa Lusia Medan. The population of this study were adolescent girl with a total sample 74 using total sampling technique. The research material in the form scales and height measurements to measure boddy mass index, quesioner for iron consumption patterns and consumption habits of Fe tablet and hemoglobin levels were examined using haemometer digital. Data analysis used chi square test (α=0,005).The results of the research are variabels related to anemia incidence are body mass index (sig=0,019), iron consumption patterns (sig=0,017), Fe tablet consumption habit (sig=0,045). Conclusion this study is factor causing anemia in adolescent girls is the pattern of iron consumption. Adolescent girl who have irreguler iron consumption pattern have an oppurtunity to experience anemia of 4,250 compared to adolescent girl who have reguler iron consumption patterns.

Spectrum of Pathological Lesions in Uterine Specimens with Focus on Gestational Trophoblastic Disease in Karachi

2020 ◽  
Keyword(s):  
Hydatidiform Mole ◽  
Endometrial Stromal Sarcoma ◽  
Gestational Trophoblastic Disease ◽  
Cross Sectional Study ◽  
Reproductive Age ◽  
P Value ◽  
Cross Sectional ◽  
Endometrial Polyps ◽  
Trophoblastic Disease ◽  
Proliferative Endometrium

Background: The most common benign pathological lesion in women of reproductive age is uterine leiomyoma. Gestational trophoblastic disease includes tumors and tumor like lesions originating from trophoblastic tissue. The aim of this study was to find the spectrum of molar pregnancy and uterine pathologies focusing on gestational trophoblastic disease as no study has been done in the past few years. Methods: Endometrial and uterine specimens of patients (n=436) between the ages of 15-65 years were collected from a private hospital in Karachi from December 2018 to December 2019. This cross-sectional study was carried out by pathological diagnosis of patients’ samples under light microscopy using hematoxylin and eosin staining. Stratification was done about age and nature of specimen to control the effect modifiers. The post stratification Chi square test was applied and p value <0.05 was considered significant. Results: Mean age of the patients was 36.1 years ±7.8. Total 436 uterine biopsies included 260(59.6%) hysterectomies, 56(12.8%) endometrial curetting’s, 117(26.8%) evacuation specimens and 3(0.7%) polypectomies. Common pathologies included 124(28.4%) leiomyomas, 61(14%) proliferative endometrium, 52(11.9%) adenomyosis and 32(7.3%) endometrial polyps. Gestational trophoblastic disease was seen in 9(2.06%). Seven (87.5%) were partial hydatidiform moles, one (12.5%) exaggerated placental site reaction and one choriocarcinoma. Mole was common between 26-30 years with mean age of 27.2 years and prevalence was 6/100 abortions. Conclusion: Leiomyoma was the commonest (28.4%) uterine pathology followed by proliferative endometrium (14.5%). However, endometrial stromal sarcoma and endometriosis were found 0.2% each. High prevalence of mole was seen in this study. Partial mole was most common and choriocarcinoma was least common. Keywords: Hydatidiform Mole; Pathology; Prevalence.

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