scholarly journals Exogenous Probiotics Improve Fermentation Quality, Microflora Phenotypes, and Trophic Modes of Fermented Vegetable Waste for Animal Feed

2021 ◽  
Vol 9 (3) ◽  
pp. 644
Author(s):  
Guilin Du ◽  
Jiping Shi ◽  
Jingxian Zhang ◽  
Zhiguo Ma ◽  
Xiangcen Liu ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Crude Protein ◽  
Animal Feed ◽  
Anaerobic Fermentation ◽  
Vegetable Production ◽  
Vegetable Waste ◽  
Promising Strategy ◽  
Fermented Feed ◽  
Fermentation Quality ◽  
Leaf Vegetable

The fermentation of leaf vegetable waste to produce animal feed reduces the environmental impact of vegetable production and transforms leaf vegetable waste into a commodity. We investigated the effect of exogenous probiotics and lignocellulose enzymes on the quality and microbial community of fermented feed (FF) produced from cabbage waste. The addition of exogenous probiotics resulted in increased crude protein (CP) content (p < 0.05), better odor (moderate organic acid and ethanol, with low ammonia-N, p < 0.05), and a lower relative abundance (RA) of pathogens (below 0.4%, p < 0.05) in FF, compared to without. With the addition of exogenous probiotics, only Pediococcus and Saccharomyces were enriched and symbiotic in FF; these were the keystone taxa to reduce the abundance of aerobic, form-biofilms, and pathogenic microorganisms, resulting in an efficient anaerobic fermentation system characterized by facultative anaerobic and Gram-positive bacterial communities, and undefined saprotroph fungal communities. Thus, inoculation of vegetable waste fermentation with exogenous probiotics is a promising strategy to enhance the biotransformation of vegetable waste into animal feed.

scholarly journals Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing

2020 ◽  
Author(s):  
Zongfu Hu ◽  
Deying Ma ◽  
huaxin Niu ◽  
Jie Chang ◽  
Jianhua Yu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Bacterial Community ◽  
High Throughput ◽  
Bacterial Communities ◽  
Crude Protein ◽  
High Throughput Sequencing ◽  
Ammonium N ◽  
Enzyme Combination ◽  
Fermentation Quality ◽  
Dominant Bacteria

Abstract This study aimed to evaluate the effects of enzymes (cellulase combined with galactosidase),, and the combination of these enzymes with Lactobacillus plantarum (LP) on bacterial diversity using high-throughput sequencing. Alfalfa forages were treated without or with cellulase + ɑ-galactosidase (CEGA), cellulase + LP (CELP), ɑ-galactosidase + LP (GALP). After 56 days of ensiling, All the treated silages exhibited improved fermentation quality as reflecting by decreased pH, ammonium-N and increased lactic acid levels compared to the control silage. Enzymatic treatment improved nutrients value by increased the level of crude protein and decreased the neutral detergent fibre (NDF) level. Treatment of the silage significantly changed the bacterial community, as determined by the PCoA test. LAB dominated the bacterial community of the treated silage after ensiling. The dominant bacteria from Garciella, Enterococcus, Lactobacillus and Pediococcus in control silage changed to Lactobacillus and Pediococcus in CEGA silage, and Lactobacillus in CELP and GALP silages. Collectively, enzymes and enzyme in combination with inoculants both greatly increased the abundance of LAB, with Enterococcus, Lactobacillus and Pediococcus in enzymes only silge (CEGA) and Lactobacillus in enzyme combination with inoculants silage (CELP and GALP).

scholarly journals Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zongfu Hu ◽  
Deying Ma ◽  
Huaxin Niu ◽  
Jie Chang ◽  
Jianhua Yu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Crude Protein ◽  
High Throughput Sequencing ◽  
Protein Levels ◽  
Nutrient Value ◽  
Ammonium N ◽  
Fermentation Quality ◽  
Dominant Bacteria

AbstractThe goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA), cellulase + LP (CELP), or ɑ-galactosidase + LP (GALP). After 56 days of ensiling, all treated silages exhibited improved fermentation quality, as reflected by decreased pH, ammonium-N and increased lactic acid levels compared to the control silage (P < 0.05). Enzymatic treatment improved nutrient value by increasing crude protein levels and decreasing neutral detergent fibre (NDF) levels (P < 0.05). Silage treatment significantly altered the bacterial community, as determined by PCoA (P < 0.05). Lactic acid bacteria (LAB) dominated the bacterial community of the treated silage after ensiling. The dominant bacteria changed from Garciella, Enterococcus, Lactobacillus and Pediococcus in the control silage to Lactobacillus and Pediococcus in the CEGA silage and Lactobacillus in the CELP and GALP silages. Collectively, these results suggest that treatment with both enzymes alone and in combination with inoculants greatly increased the abundance of LAB, with Enterococcus, Lactobacillus and Pediococcus observed in the silage treated with enzymes alone (CEGA) and Lactobacillus observed in the silage treated with a combination of enzymes and inoculants (CELP and GALP).

scholarly journals Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing

2020 ◽  
Author(s):  
Zongfu hu ◽  
Deying Ma ◽  
Huaxin Niu ◽  
Jie Chang ◽  
Jianhua Yu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Bacterial Community ◽  
High Throughput ◽  
Bacterial Communities ◽  
Crude Protein ◽  
High Throughput Sequencing ◽  
Nutrient Value ◽  
Ammonium N ◽  
Fermentation Quality ◽  
Dominant Bacteria

Abstract This study aimed to evaluate the effects of enzymes (cellulase combined with galactosidase), and the combination of these enzymes with Lactobacillus plantarum (LP) on bacterial diversity using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA), cellulase + LP (CELP), or ɑ-galactosidase + LP (GALP). After 56 days of ensiling, all the treated silages exhibited improved fermentation quality, as reflected by decreased pH, ammonium-N and increased lactic acid levels compared to the control silage. Enzymatic treatment improved nutrient value by increasing the level of crude protein and decreasing the neutral detergent fibre (NDF) level. Treatment of the silage significantly changed the bacterial community, as determined by the PCoA test. LAB dominated the bacterial community of the treated silage after ensiling. The dominant bacteria from Garciella, Enterococcus, Lactobacillus and Pediococcus in the control silage changed to Lactobacillus and Pediococcus in the CEGA silage, and Lactobacillus in the CELP and GALP silages. Collectively, enzymes and enzymes in combination with inoculants both greatly increased the abundance of LAB, with Enterococcus, Lactobacillus and Pediococcus in the silage with enzymes only (CEGA) and Lactobacillus in the silage with a combination of enzymes and inoculants (CELP and GALP).

scholarly journals Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing

2020 ◽  
Author(s):  
zongfu hu ◽  
Deying Ma ◽  
huaxin Niu ◽  
Jie Chang ◽  
Jianhua Yu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Crude Protein ◽  
High Throughput Sequencing ◽  
Protein Levels ◽  
Nutrient Value ◽  
Ammonium N ◽  
Fermentation Quality ◽  
Dominant Bacteria

Abstract The goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA), cellulase + LP (CELP), or ɑ-galactosidase + LP (GALP). After 56 days of ensiling, all treated silages exhibited improved fermentation quality, as reflected by decreased pH, ammonium-N and increased lactic acid levels compared to the control silage (P < 0.05). Enzymatic treatment improved nutrient value by increasing crude protein levels and decreasing neutral detergent fibre (NDF) levels (P < 0.05). Silage treatment significantly altered the bacterial community, as determined by PCoA (P < 0.05). Lactic acid bacteria (LAB) dominated the bacterial community of the treated silage after ensiling. The dominant bacteria changed from Garciella, Enterococcus, Lactobacillus and Pediococcus in the control silage to Lactobacillus and Pediococcus in the CEGA silage and Lactobacillus in the CELP and GALP silages. Collectively, these results suggest that treatment with both enzymes alone and in combination with inoculants greatly increased the abundance of LAB, with Enterococcus, Lactobacillus and Pediococcus observed in the silage treated with enzymes alone (CEGA) and Lactobacillus observed in the silage treated with a combination of enzymes and inoculants (CELP and GALP).

scholarly journals Co-Occurrence of Regulated and Emerging Mycotoxins in Corn Silage: Relationships with Fermentation Quality and Bacterial Communities

Toxins ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 232
Author(s):  
Antonio Gallo ◽  
Francesca Ghilardelli ◽  
Alberto Stanislao Atzori ◽  
Severino Zara ◽  
Barbara Novak ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Bacterial Communities ◽  
Dry Matter ◽  
Kojic Acid ◽  
Corn Silage ◽  
Fungal Metabolites ◽  
Fusarium Mycotoxins ◽  
Low Concentrations ◽  
Fermentation Quality ◽  
Low Levels

Sixty-four corn silages were characterized for chemicals, bacterial community, and concentrations of several fungal metabolites. Silages were grouped in five clusters, based on detected mycotoxins, and they were characterized for being contaminated by (1) low levels of Aspergillus- and Penicillium-mycotoxins; (2) low levels of fumonisins and other Fusarium-mycotoxins; (3) high levels of Aspergillus-mycotoxins; (4) high levels of non-regulated Fusarium-mycotoxins; (5) high levels of fumonisins and their metabolites. Altersetin was detected in clusters 1, 3, and 5. Rugulusovin or brevianamide F were detected in several samples, with the highest concentration in cluster 3. Emodin was detected in more than 50.0% of samples of clusters 1, 3 and 5, respectively. Kojic acid occurred mainly in clusters 1 and 2 at very low concentrations. Regarding Fusarium mycotoxins, high occurrences were observed for FB3, FB4, FA1, whereas the average concentrations of FB6 and FA2 were lower than 12.4 µg/kg dry matter. Emerging Fusarium-produced mycotoxins, such as siccanol, moniliformin, equisetin, epiequisetin and bikaverin were detected in the majority of analyzed corn silages. Pestalotin, oxaline, phenopirrozin and questiomycin A were detected at high incidences. Concluding, this work highlighted that corn silages could be contaminated by a high number of regulated and emerging mycotoxins.

scholarly journals Chemical Composition of Earthworm (Eisenia foetida) Co-Dried with Vegetable Meals as an Animal Feed

2019 ◽  
Vol 16 (2) ◽  
pp. 79-92
Author(s):  
Kirenia Pérez-Corría ◽  
Aroldo Botello-León ◽  
Abril Karina Mauro-Félix ◽  
Franklin Rivera-Pineda ◽  
María Teresa Viana ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Dry Matter ◽  
Animal Species ◽  
Animal Feed ◽  
Eisenia Foetida ◽  
Corn Meal ◽  
Feed Ingredient ◽  
Nitrogen Free Extract ◽  
Market Requirement

To evaluate the chemical composition of the earthworm (Eisenia foetida) co-dried (EW) with vegetable meals (VM) as animal feed ingredient, the blends were mixed with wheat bran (WB), rice powder (RP), corn meal (CM) and soy cake meal (SCM) in proportions of 85:15; 75:25 and 65:35. The dry matter (DM), crude protein (CP), crude fat (CFA), crude fiber (CF), ashes and nitrogen-free extract (NFE) of the ingredients and final mixtures were determined. All the mixtures resulted with a high content of DM (≥90.00 %). No significant differences among the proportions were revealed (P>0.05). In addition, the higher inclusion of the earthworm in the proportions (85:15) increased (P<0.05) the CP (54.70 %), CFA (7.28 %), and ashes (10.20 %), mainly when mixed with SCM, CM, and RP, respectively. However, the use of vegetable meals proportionally increased the CF (7.31 %), and NFE (52.62 %), mainly with the proportion of 65:35 and with RP and CM, respectively (P<0.05). The results showed that the vegetable meals (WB, RP, CM, and SCM) are useful to co-dry the earthworm to be use for animal feed. It is concluded that the most appropriate proportion (VM:EW) will depend on the animal species, productive stage and market requirement.

scholarly journals Utilization of Local Plant as Fermented Feed and Supplements for Livestock in Sendang Village, Wonogiri District

2018 ◽  
Vol 4 (1) ◽  
pp. 61
Author(s):  
Yeni Farida ◽  
Heru Sasongko ◽  
. Sugiyarto
Keyword(s):  
Herbal Medicine ◽  
Medicinal Plants ◽  
Animal Feed ◽  
Dry Season ◽  
Feed Cost ◽  
Fermented Feed ◽  
Local Plant ◽  
Three Stages ◽  
The Cost ◽  
Growth Of Animals

Raising livestock has been used as sidejob by farmers in Sendang because of availability of feed is problem, especially in the dry season. The utilization of local plant is expected to reduce the proportion of the cost for animal feed cost. In addition, supplementation of feed or herbal medicine is expected to accelerate the growth of animals to increase farmers' profits. This program aimed to provided solutions for feed endurance as well as provided knowledge to farmers about the use of medicinal plants to accelerate the growth of livestock. This program consists of three stages as followed: socialization and counseling, fermentation and herbal weightlifting ration formulation training and then evaluation. This program conducted for 45 days. The result was farmer awareness of local feed potency in Sendang village, increasing of farmer understanding about fermentation and supplement ration, ration formulation utilized directly by participant. To support the sustainability of the program, team created modules, supervised and coordinated with the head of the farmer group.

scholarly journals Kecernaan Nutrien pada Babi Lokal Periode Pertumbuhan yang Diberi Ransum Mengandung Biji Asam Biokonversi Spontan (NUTRIENTS DIGESTIBILITY IN GROWING LOCAL PIG FED WITH DIET COMPOSED OF SPONTENOUS BIOCONVERTED-TAMARIND SEED)

2018 ◽  
Vol 18 (4) ◽  
pp. 610
Author(s):  
Redempta Wea ◽  
I Gusti Komang Oka Wirawan ◽  
Bernadete Barek Koten
Keyword(s):  
Body Weight ◽  
Crude Protein ◽  
Dry Matter ◽  
Animal Feed ◽  
Block Design ◽  
Nutrient Digestibility ◽  
Tamarind Seed ◽  
Randomized Block Design ◽  
Range Test ◽  
Nutrients Digestibility

A research aimed to evaluate nutrients digestibility in growing local male pig fed spontaneous bioconverted-tamarind seed has been done in animal feed technology laboratory of Kupang State Agricultural Polytechnic.The research used 25 local male grower pigs with body weight around 3-6 kg. The study used a randomized block design with five treatments, i.e. R0 = ration using tamarind seeds without bioconversion, R1 = ration using spontaneous bioconversion tamarinds for 24 hours, R2 = rations using spontaneous bioconversion tamarinds for 48 hours, R3 = rations using bioconversion tamarinds spontaneous for 72 hours, and R4 = rations using spontaneous bioconversion tamarinds for 96 hours. Each treatment was repeated five times in which each of them used five pigs. Parameters observed were nutrient digestibility (dry matter, crude protein, crude fat, crude fiber, and ash). The data were analyzed by using variance and continued by Duncan’s multiple range test to determine the differences between treatments. The results showed that the time of spontaneous bioconversion of tamarind seeds increased the digestibility of tamarind seed nutrients and it was concluded that the best time of spontaneous bioconversion was for 72 hours.

scholarly journals Effects of Replacing Groundnut Cake with Blood Vegetable Waste Meal in the Diets of Weaner Rabbits

2012 ◽  
Vol 2012 ◽  
pp. 1-4
Author(s):  
Adedayo Abiodun Adeniji
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Feed Intake ◽  
Crude Protein ◽  
Body Weight Gain ◽  
Control Diet ◽  
Vegetable Waste ◽  
Gain Ratio ◽  
Groundnut Cake ◽  
Ad Libitum

A total of seventy-two weaner rabbits of eight weeks of age were used to assess the effects of replacing groundnut cake (GNC) with blood vegetable waste meal (BVWM) in the diets of rabbits. The BVWM was fed to replace dietary GNC at 0, 15, 30, and 45%, with GNC being 15% in the control diet. The four experimental diets were fed ad libitum for a period of eight weeks. BVWM was analyzed to contain a crude protein value of 62.35%. There were comparable feed intake values by rabbits on all the diets although the rabbits in the higher replacement levels of BVWM tended to have consumed more of the feed. There were significant increases (P<0.05) in body weight gain by rabbits as the BVWM level increased in the diet. Similarly, the feed to gain ratio improved and nitrogen digestibility increased (P<0.05) with higher levels of BVWM in the diet. This study shows that rabbits can tolerate the 45% BVWM replacement of groundnut cake effectively.

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