Carotene as a hematopoietic agent Binet Strumza (Pg. Med. No. 3, 1932)

Carotene, a coloring matter of many plants (carrots, peaches, spinach, chestnuts, trees, etc.) and animal products (oil, egg yolk, etc.) is considered essential for the growth of animals.

Effects of Dietary Supplementation of gEGF on the Growth Performance and Immunity of Broilers

EGF has been shown to stimulate the growth of animals. In this study, the content of EGF in chicken embryos (gallus EGF, gEGF) aged from 1 to 20 days of incubation were determined by ELISA kit, and the 5-day-old chicken embryos with the highest content of 5593 pg/g were selected to make gEGF crude extracts. A total of 1500 1-day-old Xianju chickens were randomly divided into five groups with six replicates of 50 chickens each. The control group was fed a basal diet, and other treatment diets were supplemented with 4, 8, 16 and 32 ng/kg gEGF crude extract, respectively. The experiment lasted for 30 days. Chicks were harvested at the end of the experiment, and liver, spleen, thymus, bursa and serum samples were collected. Results showed that average daily gain (ADG) and average daily feed intake (ADFI) of 16 ng/kg group were higher than those in the control group (p < 0.05). The serum uric acid (UA) of the 16 ng/kg group was reduced (p < 0.01), and the serum alkaline phosphatase (AKP) of the 16 ng/kg group increased (p < 0.01). The gEGF extract also increased chick’s antioxidant capacity, decreased malondialdehyde (MDA) and increased catalase (CAT) in the liver and serum of 16 ng/kg groups in compared to the control group (p < 0.01). Furthermore, immunity was improved by the addition of gEGF to broiler diets. The serum immunoglobin A (IgA) content of 8 and 16 ng/kg groups and the serum immunoglobin M (IgM) content of 4 and 8 ng/kg groups were increased (p < 0.05) compared to the control group. The bursa index of each experimental group was higher than the control group (p < 0.01). These findings demonstrate that the crude extract of gEGF prepared in this experiment could improve the growth performance, antioxidant capacity and immunity of broilers.

DYNAMICS OF LIVE WEIGHT AND MEAT QUALITY OF PIGS OF DIFFERENT GENOTYPES WHEN FEEDING DRY BLOOD

Проблема и цель. Целью исследований явилось изучение в условиях промышленной технологии новых методов повышения продуктивных качеств свиней за счёт использования высокопротеинового корма из сухой крови свиней. Методология. Для экспериментов методом сбалансированных групп были составлены 8 групп свиней в возрасте 28 дней, по 30 голов каждая. В ходе исследований проводили оценку роста молодняка, изучали мясные качества свиней и физические свойства мяса. При этом нами учитывались такие показатели как абсолютный и среднесуточный приросты, живая масса, убойная масса, убойный выход, площадь «мышечного глазка», толщина шпига, длинна туши, влагоудерживающая способность и цветность мяса. Результаты. В конце опытного периода животные всех опытных групп, получавшие с рационом сухую кровь свиней, превосходили по массе свиней соответствующих контрольных групп. По породе йоркшир – на 2,1 кг, по породе ландрас – на 3,1 кг, по породе дюрок – на 4,3 кг, по гибридной группе – на 2,8 кг. Наиболее высокими показателями мясных качеств характеризовались животные опытных групп. Так, свиньи порды йоркшир превосходили животных контрольной группы той же породы на 0,6 %, по породе ландрас – на 1,1 %, по породе дюрок – на 0,6 %, по гибридам – на 0,8 %. Более высокая влагоудерживающая способность была отмечена в мясе животных породы ландрас и гибридной группы – 57,2 % и 56,8 %. Показатель рН мышечной ткани животных опытных групп находился в пределах нормы. Цветность мяса свиней разных генотипов в результате скармливания сухой крови свиней повышалась. Заключение. Таким образом, включение в рационы свиней сухой крови свиней оказывает положительное влияние на рост животных и улучшает мясные качества свиней и физические показатели мяса. Problem and goal. The purpose of the research was to study new methods of increasing the productive qualities of pigs by using high-protein feed from dry pig blood in the conditions of industrial technology. Methodology. For the experiments, 8 groups of pigs aged 28 days, 30 heads each, were made up by the method of balanced groups. In the course of the research, the growth of young animals was evaluated, the meat qualities of pigs and the physical properties of meat were studied. At the same time, we took into account such indicators as absolute and average daily gains, live weight, slaughter weight, slaughter yield, the area of the "muscle eye", the thickness of the ham, the length of the carcass, the moisture-retaining ability and the color of the meat. Results. At the end of the experimental period, the animals of all experimental groups that received dry pig blood with the diet were superior in weight to the pigs of the corresponding control groups. For the Yorkshire breed - by 2.1 kg, for the Landrace breed - by 3.1 kg, for the Duroc breed - by 4.3 kg, for the hybrid group - by 2.8 kg. The animals of the experimental groups were characterized by the highest indicators of meat qualities, so Yorkshire pigs outperformed the animals of the control group of the same breed by 0.6 %, for the Landrace breed-by 1.1 %, for the Duroc breed – by 0.6%, for hybrids – by 0.8 %. Higher water-holding capacity was observed in the meat of Landrace and hybrid group animals – 57.2 % and 56.8 %. The pH of the muscle tissue of the experimental animals was within the normal range. The color of pig meat of diferent genotypes increased as a result of feeding dry pig blood. Conclusion. Thus, the inclusion of dry pig blood in the diets of pigs has a positive efect on the growth of animals and improves the meat quality of pigs and the physical performance of meat.

Post-weaning growth of cavies (Cavia porcellus) according to the inclusion level of ginger (Zingiber officinale) meal in the diet as a feed additive

The use of phytobiotics in feeding monogastric herbivores has several interests, including the stabilization of the caecal flora and especially the improvement of the growth of animals thanks to a better exploitation of feed resources. Thus a trial was conducted with an objective to evaluate the feed intake and post weaning growth performances of cavy according to the inclusion level of ginger meal. A total of 40 guinea pigs of 3 weeks of age were divided into 4 lots, and subjected to rations consisting of Trypsacum laxum served ad libitum, associated with the compound feed containing 0; 0.5; 0.75 and 1% ginger powder (T0, T1, T2 and T3). This study showed that the total intake of DM, MO and CB from forage was comparable between rations T0, T1 and T2 and significantly higher than those of ration T3. At weaning, average animal weights of all rations were comparable. However, at 8 weeks of age, the T0, T1 and T3 rations yielded comparable average weights, but significantly higher than those of the T2 animals, i.e., 272 g; 298.80 g; 285.25 g and 221.83 g, respectively. The T0, T1 and T3 diets recorded total gains that were comparable but significantly higher than those obtained with animals in Lot T2. Ginger powder can be included up to 0.75% in the diet as a feed additive for improved ingestion and 0.5% for post-weaning growth. L’usage des phytobiotiques dans l’alimentation des herbivores monogastriques présente plusieurs intérêts parmi lesquels la stabilisation de la flore caecale et surtout l’amélioration de la croissance des animaux grâce à une meilleure valorisation des ressources alimentaire. C’est ainsi que des essais ont été conduit avec pour objectif d’évaluer l’ingestion er la croissance post-sevrage des cobayes en fonction du niveau de la poudre de gingembre. Un total de 40 cobayes de 21 jours d’âge était reparti en 4 lots, et soumis à des rations constituées de Trypsacum laxum servi ad libitum, associé à l’aliment composé contenant 0 ; 0,5 ; 0,75 et 1% de poudre de gingembre (T0, T1, T2 et T3). Il ressort de cette étude que les ingestions totales de la MS, de la MO et de la CB du fourrage, ont été comparables entre les rations T0, T1 et T2 et significativement plus élevées que celle de la ration T3. Au sevrage, les poids moyens des animaux étaient comparables pour toutes les rations. Cependant à 8 semaines d’âge, les rations T0, T1 et T3 ont permis d’obtenir les poids moyens comparables, mais significativement plus élevés que ceux des animaux lot T2, soit respectivement 272 g ; 298,80 g ; 285,25 g et 221,83 g. Les rations T0, T1 et T3 ont permis d’enregistrer les gains totaux comparables, mais significativement élevés par rapport à ceux obtenus avec les animaux du lot T2. La poudre de gingembre peut être incluse jusqu’à 0,75% dans la ration comme additif alimentaire pour l'amélioration de l’ingestion et à 0,5% pour la croissance post-sevrage.

Preparation of Sodium Lignosulfonate/Chitosan Adsorbent and Application of Pb2+ Treatment in Water

Industrial wastewater has brought huge disasters to water resources and soil and has seriously affected the growth of animals and plants. There is an urgent need for a green and efficient adsorbent to solve the problem of water pollution. Sodium lignosulfonate and chitosan undergo free radical polymerization to form a lignin/chitosan adsorbent, which is used to treat Pb2+ in water pollution. An orthogonal experiment was used to optimize the content of sodium lignosulfonate, chitosan, cross-linking agent and initiator to obtain the adsorbent with the best adsorption performance. The adsorbents were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), thermal analysis and zeta potentiometry. The influence of different conditions on the adsorption of heavy metal ions by lignosulfonate/chitosan adsorbent was explored, and a kinetic and isotherm model was established. The results showed that the adsorption capacity of Pb2+ was 345 mg g−1 when the adsorbent was 0.01 g, the concentration of heavy metal ions was 100 mg L−1 and pH was 7. The adsorption process of lignosulfonate/chitosan is a kind of spontaneous adsorption mode, which is mainly composed of electrostatic adsorption and chemical adsorption.

Study of the influence of the feeding type of calves during the milk fattening period on the subsequent growth and development of bulls up to 12 months of age

Research has been carried out on the intensive production of beef when raising dairy bulls up to 12 months of age drinking mainly dairy feed in the first 5 months of life (in group 1) and with the inclusion of a starter compound feed in the diet (in group 2). Different types of bull calves rearing in the first 5 months of life led to significant differences in the growth and development of the whole organism and all its individual organs and tissues, mainly in favour of bull calves raised with replacing part of the milk with vegetable feed, and subsequently had a decisive influence in the period from 6 up to 12 months of their life with the same type of feeding and maintenance. It has been established that the rearing system with a decrease in the drinking of whole milk and abstinence of the supply of roughage for the first 2 months of life, promotes the the growth of animals for a twelve-month period of life, leads to an increase in the yield of veal and beef meat, a decrease in feed consumption per unit of weight gain, and increasing the profitability of production by 8-9%, therefore it is recommended for production.

CHANGE IN BLOOD BIOCHEMICAL INDICATORS AND INTESTINAL MORPHOMETRIC INDICATORS BECAUSE OF PROPOLIS PREPARATION INCLUSION IN DIETS

The research results in a number of cases allow us to judge about the change in the functional activity of the studied organs after the use of the drug. The obtained factual material significantly complements the information on the biological properties of the aqueous-alcoholic propolis emulsion. In practical veterinary medicine, research results can be taken into account when prescribing propolis for therapeutic and prophylactic purposes, since histological analysis of intestinal sections did not reveal destructive or pathological changes in all compared groups. In the field of zootechnics, the obtained practical material makes it possible to concretize the stimulating effect of the preparation in terms of stimulating the growth of animals depending on the age of pigs. So, as a result of the study, it was found that the most characteristic histological changes in the structural components of the intestinal wall are observed in the ileum of piglets and pigs of the experimental groups. The height of the villi of this intestine in piglets of the experimental piglet is 280.97 ± 3.37 μm, and in the control piglet – 193.89 ± 2.66 μm (p <0.001). In pigs receiving the drug, the villi also have a greater height – 256.89 ± 1.62 μm versus 163.96 ± 0.61 μm in the control group. In piglets and pigs of the control groups, the shape of the villi of the ileum is finger-shaped or bottle-shaped, while in the animals of the experimental groups their forms are more differentiated – bottle-shaped, leaf-shaped and dome-shaped. This will allow young animals receiving propolis to better absorb the nutrients of the diet and gain weight faster. The biochemical parameters of the blood of all animals in the dynamics of the experiment were within the physiological norm. The only exception was a slight decrease below the norm in piglets in the control group with reserve alkalinity of 44.81 ± 0.98 vol. % CO2, observed at the beginning of the experiment. The research results are of interest to researchers developing oral propolis preparations.

A de novo frameshift mutation in ZEB2 causes polledness, abnormal skull shape, small body stature and subfertility in Fleckvieh cattle

Polledness in cattle is an autosomal dominant trait. Previous studies have revealed allelic heterogeneity at the polled locus and four different variants were identified, all in intergenic regions. In this study, we report a case of polled bull (FV-Polled1) born to horned parents, indicating a de novo origin of this polled condition. Using 50K genotyping and whole genome sequencing data, we identified on chromosome 2 an 11-bp deletion (AC_000159.1:g.52364063_52364073del; Del11) in the second exon of ZEB2 gene as the causal mutation for this de novo polled condition. We predicted that the deletion would shorten the protein product of ZEB2 by almost 91%. Moreover, we showed that all animals carrying Del11 mutation displayed symptoms similar to Mowat-Wilson syndrome (MWS) in humans, which is also associated with genetic variations in ZEB2. The symptoms in cattle include delayed maturity, small body stature and abnormal shape of skull. This is the first report of a de novo dominant mutation affecting only ZEB2 and associated with a genetic absence of horns. Therefore our results demonstrate undoubtedly that ZEB2 plays an important role in the process of horn ontogenesis as well as in the regulation of overall development and growth of animals.

Morphological indicaters of the gastrointestinal tract of young pigs during feeding of the PVMS “Efiprot”

The problem of increasing of livestock and poultry has led scientists to look for measures to improve productivity and reduce costs. One way to solve this problem is to use balancing supplements in the feeding. The study of feeding by protein-vitamin-mineral feed supplement “Efiprot” was carried out on three groups of young white pigs, 12 heads in each group. The experiment consisted of leveling of 15 and a basic period of 127 days. During the rearing period, the animals consumed the feed according to the growth phases, increasing the amount from 1 kg/head (8–15 kg of live weight), up to 1.5 kg/head per day (15–35 kg of live weight) and from 2 kg/head (35–65 kg of live weight) and up to 2.5 kg/head. per day (65–110 kg of live weight). While using in feeding of young pigs of PVMS “Efiprot”, a positive productive effect was obtained. Thus, when introduced to the diet of the tested yong pig the PVMS, the average daily growth of animals has increased by 33–58 g. Accordingly, has increased the final live weight of the animals by 4.05–7.18 kg against the control level. Due to the result of the studies, carrying out the control slaughter, it was found that feeding of the protein-vitamin-mineral supplement “Efiprot” for young pigs on rearing does not have a probable effect on the weight of the stomach, but causes changes in the thickness of the wall of the cardiac zone by 0.24 mm (2.75 %), and reducing the wall thickness relative to the control by 1.04 mm (11.91 %). In the fundus zone of the stomach, there is a tendency to thicken the walls due to the mucous and sere-muscular membranes by 0.36 mm (6.75 %) and 0.35 mm (6.56 %). In the study of the pyloric zone, the thickness of the walls was decreased by 0.46 mm (3.13 %) and 0.73 mm (4.97 %) compared to the control group.The reaction of the intestine to feeding of “Efiprot” to young pigs is caused by an increase in weight and length of 0.06 – 0.2 kg and 0.5 m – 0.83 m. Also it is shown a slight decrease in the thickness of the hollow shell by 0.5 mm (18.3 %) in the second group of animals 0.21 mm (7.69 %). A significant difference was found in the structure of the colon of experimental animals, where there was an increase in wall thickness in the first group by 0.13 mm (5.75 %) and a decrease in the second group by 0.16 mm (7.07 %).

Supplementation levels for suckling female calves under grazing: productive and nutritional performance and metabolic profile

The objective in this study was to evaluate the effects of supplementation levels on productive and nutritional performance and metabolic profile in suckling female calves under grazing. Forty female calves (averaging 3.5±0.06 months and 127.3±2.68 kg), and their respective dams were distributed in a completely randomized design with two treatments and twenty replicates. The treatments were 1) 4 g kg-1 body weight (BW) of supplement or 2) 6 g kg-1 BW of supplement. Forage and organic matter (OM) intake did not affect (P > 0.05) by levels of supplement, though crude protein and non-fibrous carbohydrates intake were greater (P < 0.05) by increasing supplementation level. There was no effect (P > 0.05) the supplementation levels on OM and CP digestibility. The metabolic profile of the animals was not affected (P > 0.05) by supplementation level. Average daily gain, longissimus dorsi area, fat thickness over rump of the animals did not affect (P > 0.05) by levels of supplement. However, there was trend of increasing (P=0.074) in fat thickness over loin by increase the supplementation level. Although the body growth of animals was similar (P > 0.05) between treatments, there was observed a trend of increase (P=0.064) in ratio BW:Height at the withers by increasing supplementation levels. In conclusion, increasing the supplementation level of 4 to 6 g kg-1 of BW, not improve the productive and nutritional performance and metabolic status in female calves under grazing on creep-feeding system.