scholarly journals Antioxidant and Hepatoprotective Activity of Veronica ciliata Fisch. Extracts Against Carbon Tetrachloride-Induced Liver Injury in Mice

Molecules ◽  
2014 ◽  
Vol 19 (6) ◽  
pp. 7223-7236 ◽  
Author(s):  
Li Yin ◽  
Lei Wei ◽  
Rao Fu ◽  
Lisheng Ding ◽  
Yiran Guo ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Hepatoprotective Activity

scholarly journals Hepatoprotective Effect of the Fruits of Polygonum orientale L. Against Carbon Tetrachloride-Induced Liver Fibrosis in Mice

2020 ◽  
Vol 15 (11) ◽  
pp. 1934578X2097150
Author(s):  
Yung-Jia Chiu ◽  
Kun-Chang Wu ◽  
Jen-Chieh Tsai ◽  
Chun-Pin Kao ◽  
Jung Chao ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Growth Factor ◽  
Liver Injury ◽  
Transforming Growth Factor ◽  
Smooth Muscle Actin ◽  
Transforming Growth Factor Β ◽  
Serum Levels ◽  
Hepatoprotective Activity ◽  
Tissue Growth ◽  
Factor Α

The aim of this study was to evaluate the hepatoprotective effects of the fruits of Polygonum orientale L. (POE) against fibrosis in carbon tetrachloride (CCl4)-induced liver injury. Bioactive components of POE were identified using liquid chromatography (LC)-mass spectrometry (MS)/MS by comparison with standards. Treatment with either silymarin (200 mg/kg) or POE (0.5 and 1.0 g/kg) caused significant decreases in the serum levels of enzymes and reduced the extent of liver lesions and fibrosis in histological analysis. POE (0.5 and 1.0 g/kg) decreased the levels of malondialdehyde, nitric oxide, proinflammatory cytokines (ie, tumor necrosis factor-α, interleukin [IL]-1β, and IL-6), an inflammatory cytokine (ie, cyclooxygenase-2), a profibrotic cytokine (ie, transforming growth factor-β), and fibrosis-related proteins (ie, connective tissue growth factor and α-smooth muscle actin) in the liver and enhanced the activities of the antioxidative enzymes superoxide dismutase, glutathione peroxidase, glutathione reductase, and catalase. Quantitative analysis of the active constituents in POE revealed an extract composition of 3.4 mg/g of protocatechuic acid, 20.8 mg/g of taxifolin, and 5.6 mg/g of quercetin. We have demonstrated that the hepatoprotective mechanisms of POE are likely to be associated with the decrease in inflammatory cytokines by increasing the activities of antioxidant enzymes. Our findings provide evidence that POE possesses a hepatoprotective activity to ameliorate chronic liver injury.

Effects and Mechanisms of Acremoniumterricola milleretal mycelium on Liver Fibrosis Induced by Carbon Tetrachloride in Rats

2011 ◽  
Vol 39 (03) ◽  
pp. 537-550 ◽  
Author(s):  
Xiao-Peng Tian ◽  
Yan-Yan Yin ◽  
Xia Li
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Fibrosis ◽  
Liver Injury ◽  
Chinese Herb ◽  
Immunoblot Analysis ◽  
Hepatoprotective Activity ◽  
Protective Effects ◽  
Sprague Dawley ◽  
Procollagen Type ◽  
Tgf Β1

Acremoniumterricola milleretal mycelium (AMM) is one of the most precious traditional Chinese medicines. It has numerous protective effects on organs, and has been used in Chinese herb prescription to treat refractory diseases. Our preliminary studies demonstrated that AMM had hepatoprotective activity in acute liver injury. We further investigated the effects of AMM on liver fibrosis in rats induced by carbon tetrachloride ( CCl 4) and explore its possible mechanisms. The animal model was established by injection with 50% CCl 4 subcutaneously in male Sprague-Dawley rats twice a week for eight weeks. Meanwhile, AMM (175, 350 and 700 mg/kg) was administered intragastrically per day until sacrifice. We found that treatment with AMM (175, 350 and 700 mg/kg) decreased CCl 4-induced elevation of serum transaminase activities, hyaluronic acid, laminin and procollagen type III levels, and contents of hydroxyproline in liver tissues. It also restored the decreased SOD and GSH-Px activities and inhibited the formation of lipid peroxidative products during CCl 4 treatment. Moreover, AMM (350 and 700 mg/kg) decreased the elevation of TGF-β1 by 19.6% and 34.3%, respectively. In the pathological study, liver injury and the formation of liver fibrosis in rates treated by AMM were improved significantly. Immunoblot analysis showed that AMM (175, 350 and 700 mg/kg) inhibited Smad 2/3 phosphorylation, and elevated inhibitor Smad 7 expression. These results suggested that AMM could protect liver damage and inhibit the progression of hepatic fibrosis induced by CCl 4, and its mechanisms might be associated with its ability to scavenge free radicals, decrease the level of TGF-β1 and block TGF-β/Smad signaling pathway.

scholarly journals Hepatoprotective activity of Chhit-Chan-Than extract powder against carbon tetrachloride-induced liver injury in rats

2014 ◽  
Vol 22 (2) ◽  
pp. 220-229 ◽  
Author(s):  
Yi-Chun Lin ◽  
Kuei-Mei Cheng ◽  
Hsin-Yu Huang ◽  
Pei-Yu Chao ◽  
Jin-Ming Hwang ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Hepatoprotective Activity

scholarly journals Hepatoprotective effects of Calotropis gigantea extract against carbon tetrachloride induced liver injury in rats

2009 ◽  
Vol 59 (1) ◽  
pp. 89-96 ◽  
Author(s):  
Gaurav Lodhi ◽  
Hemant Singh ◽  
Kamlesh Pant ◽  
Zeashan Hussain
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Lipid Peroxide ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Standard Drug ◽  
Calotropis Gigantea ◽  
Male Wistar Rats ◽  
Antioxidant Parameters ◽  
Hepatoprotective Effects

Hepatoprotective effects ofCalotropis giganteaextract against carbon tetrachloride induced liver injury in ratsEthanolic extract (50 %) of stems ofCalotropis giganteaR. Br. (Asclepiadaceae) at doses of 250 and 500 mg kg-1were studied for hepatoprotective activity in male Wistar rats with liver damage induced using carbon tetrachloride, 2 mL kg-1twice a week. The protective effect ofC. giganteaextract was compared with the standard drug silymarin. Various biochemical parameters such as aspartate amino transferase (AST), alanine amino transferase (ALT), glutathione (GSH), lipid peroxide (LPO), superoxidedismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were evaluated. The results revealed that theC. giganteaextract significantly decreased AST, ALT (p< 0.001) and lipid peroxide (p< 0.01) levels. The antioxidant parameters GSH, GPx, SOD and catalase levels were increased considerably compared to their levels in groups not treated withC. giganteaextract.

scholarly journals DEVELOPMENT OF CARBON TETRACHLORIDE-INDUCED CHRONIC HEPATOTOXICITY MODEL IN RATS AND ITS APPLICATION IN EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF SILYMARIN

2017 ◽  
Vol 10 (8) ◽  
pp. 274
Author(s):  
Gopi H Shah ◽  
Bharat G Patel ◽  
Gaurang B Shah
Keyword(s):  
Carbon Tetrachloride ◽  
Treatment Group ◽  
Liver Injury ◽  
Protein Oxidation ◽  
Liver Function Tests ◽  
Hepatoprotective Activity ◽  
Direct Bilirubin ◽  
Disease Induction ◽  
Histopathological Studies ◽  
Serum Glutamate

Objective: The objective of the present work is to develop carbon tetrachloride (CCl4)-induced chronic hepatotoxicity model in rats and its applicationin evaluation of hepatoprotective activity of silymarin.Methods: Animals were divided into four groups. Three groups were the disease induction group and 4th was the treatment group. In disease inductiongroups, chronic liver injury was induced by administration of CCl4 through intraperitoneal route (1 ml/kg) for 7-8 weeks. For treatment Group, 1 mlsilymarin suspension (orally) and CCl4 was given for 7-8 weeks. During disease induction and treatment period (7-8 weeks), blood samples werecollected and serum was separated which in turn used to analyze liver function tests such as serum glutamate oxaloacetate transaminase (SGOT),serum glutamate pyruvate transaminase (SGPT), alkaline phosphate (ALP), direct bilirubin, total protein (TP), and albumin (Alb) levels. Along withliver functional tests, tests to check cholesterol, glucose, and malondialdehyde (MDA) were also performed. Liver fibrosis and cirrhosis was quantifiedby histopathological studies of small portion of the excised liver. Model was validated by repetition of the experiment. Intermediate dissection wascarried out to measure an extent of liver damage.Result: Serum SGOT, SGPT, ALP, and direct bilirubin were found to be significantly higher in CCl4 intoxicated rats. TP and Alb were decreased, andMDA was found to be significantly higher in CCl4 intoxicated rats, which is the main end product of lipid peroxidation. Whereas in the treatment groupsilymarin improved the liver functions in CCl4 toxicated drug.Conclusion: We conclude that protein oxidation may play a role in the pathogenesis of CCl4 induced liver injury. The accumulation of oxidized proteinsmay be an early indication of CCl4 induced liver damage and silymarin found to be effective in liver injury by inhibiting protein oxidation.

Hepatoprotective Effect ofShidagonglaoon Acute Liver Injury Induced by Carbon Tetrachloride

2009 ◽  
Vol 37 (06) ◽  
pp. 1085-1097 ◽  
Author(s):  
Jung Chao ◽  
Meng-Shiou Lee ◽  
Sakae Amagaya ◽  
Jiunn-Wang Liao ◽  
Jin-Bin Wu ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Acute Liver Injury ◽  
Ethanol Extract ◽  
Neutrophil Infiltration ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Hepatoprotective Effect ◽  
Oxidative Enzymes ◽  
Tnf Α

This study investigates the hepatoprotective activity of ethanol extract from Shidagonglao roots (SDGLEtOH). The hepatoprotective effect of SDGLEtOH(20, 100 and 500 mg/kg) was analyzed on carbon tetrachloride ( CCl4)-induced acute liver injury. Rats pretreated orally with SDGLEtOH(100 and 500 mg/kg) and silymarin (200 mg/kg) for 3 consecutive days prior to the administration of a single dose of 50% CCl4(0.10 ml/100 g of bw, ip) significantly prevented the increases in the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in CCl4-treated rats. Histological analysis also showed that SDGLEtOH(100 and 500 mg/kg) and silymarin reduced the incidence of liver lesions including vacuole formation, neutrophil infiltration and necrosis of hepatocytes induced by CCl4in rats. Moreover, the SDGLEtOH(100 and 500 mg/kg) increased the activities of anti-oxidative enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRd) and decreased malondialdehyde (MDA) level in liver, as compared to those in the CCl4-treated group. Furthermore, SDGLEtOH(100 and 500 mg/kg) and silymarin attenuated the increased levels of tumor necrosis factor-α (TNF-α) in serum and nitric oxide ( NO ) in liver as compared to the CCl4-treated group. The hepatoprotective mechanisms of SDGLEtOHare likely related to inhibition of TNF-α, MDA and NO productions via increasing the activities of antioxidant enzymes (SOD, GPx and GRd). These experimental results suggest that SDGLEtOHcan attenuate CCl4-induced acute liver injury in rats.

EXPERIMENTAL EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF OXYMETHYLURACIL IN COMBINATION WITH KNOWN MEDICINES (MEXIDOL, TONAROL, ACETYLCYSTEINE)

2021 ◽  
pp. 14-20
Author(s):  
Е. F. Repina ◽  
D. O. Karimov ◽  
G. V. Timasheva ◽  
N. Yu. Khusnutdinova ◽  
L. A. Musina ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Experimental Evaluation ◽  
Acute Liver Injury ◽  
Morphological Changes ◽  
Hepatoprotective Activity ◽  
Medical Products ◽  
Corrective Effect ◽  
Different Types

The article presents the results of morphological changes in the liver of rats after acute liver injury induced by carbon tetrachloride and prophylactic correction of injuries by combinations of oxymethyluracil with different types of medical products: «Mexidol», «Tonarol» and Acetylcysteine. The best corrective effect was established after applying a combination of oxymethyluracil with «Tonarol». Thus, according to the results obtained, the studied drugs combinations can be arranged in the following sequence in accordance with decreasing of hepatoprotective activity: OMU / «Tonarol» > OMU / Acetylcysteine> OMU / «Mexidol».

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