scholarly journals Suppressive Effects of EGCG on Cervical Cancer

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2334 ◽  
Author(s):  
Ying-Qi Wang ◽  
Jian-Liang Lu ◽  
Yue-Rong Liang ◽  
Qing-Sheng Li

Cervical cancer is the fourth most common gynecological cancer worldwide. Although prophylactic vaccination presents the most effective method for cervical cancer prevention, chemotherapy is still the primary invasive intervention. It is urgent to exploit low-toxic natural anticancer drugs on account of high cytotoxicity and side-effects of conventional agents. As a natural product, (-)-epigallocatechingallate (EGCG) has abilities in anti-proliferation, anti-metastasis and pro-apoptosis of cervical cancer cells. Moreover, EGCG also has pharmaceutical synergistic effects with conventional agents such as cisplatin (CDDP) and bleomycin (BLM). The underlying mechanisms of EGCG suppressive effects on cervical cancer are reviewed in this article. Further research directions and ambiguous results are also discussed.

2017 ◽  
Author(s):  
Carolyn D. Runowicz ◽  
Andrew Quinn

With the advent of HPV DNA testing and the availability of HPV vaccinations, the recommendations and rationale for screening and prevention of cervical cancer and its precursors have undergone revision, reflecting this new knowledge and understanding of cervical intra-epithelial neoplasia and the role of HPV. This review incorporates the new guidelines and rationale for current screening guidelines for cervical cancer and in the management of patients with atypical or unsatisfactory cervical cytology. This review contains 4 figures, 2 tables, and 69 references. Key words: Cervical cancer, Gynecological cancer, HPV, HPV testing, HPV vaccine, Pap smear, HPV DNA, Human papillomavirus


2019 ◽  
Vol 53 (4) ◽  
pp. 443-452 ◽  
Author(s):  
Qing-Qing Chang ◽  
Chun-Yan Chen ◽  
Zhao Chen ◽  
Shuai Chang

Abstract Background Cervical cancer is one of the most frequent malignancies among females worldwide. Increasing evidence have indicated the participation of long noncoding RNAs (lncRNAs) in the progression and metastasis of cervical cancer. Our present study was conducted to explore the effects of lncRNA plasmacytoma variant translocation 1 (PVT1) on the progression of cervical cancer and the underlying mechanisms. Materials and methods Expressions of PVT1, miR-140-5p and Smad3 in cervical cancer cell lines were detected by qRT-PCR and western blotting. Bioinformatics analysis and luciferase assays were used to elucidate the potential correlations between PVT1, miR-140-5p and Smad3. The roles of PVT1 on the progression of cervical cancer cells were determined by transfecting sh-RNA through series function assays such as colony formation assay, wound healing assay, transwell assay. Results PVT1 and Smad3 were upregulated, and miR-140-5p was downregulated in cervical cancer cells. PVT1 could bind directly with miR-140-5p, and Smad3 was a downstream target of miR-140-5p. Inhibition of PVT1 could enhance expression of miR-140-5p, inhibit the expression of Smad3, significantly inhibited the proliferation, migration, invasion in cervical cancer cells. While transfection of miR-140-5p inhibitor could partially reverse the above changes in cervical cancer cells. Conclusions The results revealed that PVT1 could promote the proliferation and metastasis via increasing the Smad3 expression by sponging miR-140-5p, which might be a promising prognostic and therapeutic target for cervical cancer.


2019 ◽  
Vol 4 (4) ◽  
pp. 125-130
Author(s):  
Chigozie Gloria Anene-Okeke ◽  
Deborah O Aluh ◽  
Uzoamaka Henrietta Okorie

Background: Cervical cancer is the second most commonly diagnosed gynecological cancer affecting women worldwide. It is a preventable disease but still remains a leading cause of cancer deaths in developing countries like Nigeria despite the availability of preventive strategies. Undergraduate pharmacy and medical students are future health care providers who can help raise awareness and improve the knowledge of the public towards cervical cancer, its screening, prevention and treatment. The study sought to assess the knowledge, attitudes and practice of cervical cancer screening and prevention. Materials and methods: This is a cross sectional survey using questionnaire conducted in the University of Nigeria and Nnamdi Azikiwe University both in South-eastern Nigeria. Participants were conveniently sampled and the self-administered questionnaire was given to 335 consenting female pharmacy and medicine undergraduate students. Results: 335 out of the 350 undergraduate students approached responded (95.7 % response rate). The result of the study shows that the students generally had a good level of awareness of cervical cancer. The students surveyed had a mean knowledge score of 75.9062±20.265. More than a third (36.1%, n = 214) of the students had a negative attitude towards cervical cancer. Only about one in eight students reported to have had a pap smear (12.54%, n = 42). Conclusion: The students generally had a good awareness and knowledge of cervical cancer which was not translated into practice. There is a need to include public health principles into the curriculum of Pharmacy and Medical schools in Nigeria since they are future health advisors.


2018 ◽  
Author(s):  
Andrew Quinn ◽  
Carolyn D Runowicz

With the advent of HPV DNA testing and the availability of HPV vaccinations, the recommendations and rationale for screening and prevention of cervical cancer and its precursors have undergone revision, reflecting this new knowledge and understanding of cervical intra-epithelial neoplasia and the role of HPV. This review incorporates the new guidelines and rationale for current screening guidelines for cervical cancer and in the management of patients with atypical or unsatisfactory cervical cytology. This review contains 4 tables and 64 references Key words: Cervical cancer, Gynecological cancer, HPV, HPV testing, HPV vaccine, Pap smear, HPV DNA, Human papillomavirus


2021 ◽  
Vol 11 ◽  
Author(s):  
Hui Hou ◽  
Rong Yu ◽  
Haiping Zhao ◽  
Hao Yang ◽  
Yuchong Hu ◽  
...  

Cervical cancer is one of the most common gynecological cancers. Cisplatin resistance remains a major hurdle in the successful treatment of cervical cancer. Aberrant expression of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are implicated in cisplatin resistance. However, the regulatory functions of lncRNAs and miRNAs in cervical cancer cisplatin resistance and the underlying mechanisms are still elusive. Our qRT-PCR assays verified that miR-206 levels were down-regulated in cisplatin-resistant cervical cancer cells. The introduction of miR-206 sensitized cisplatin-resistant cervical cancer cells to cisplatin. Our qRT-PCR and luciferase reporter assays showed that Cyclin D2 (CCND2) was the direct target for miR-206 in cervical cancer cells. The cisplatin-resistant cervical cancer cells expressed higher CCND2 expression than the parental cells, whereas inhibition of CCND2 could sensitize the resistant cells to cisplatin treatment. Furthermore, we demonstrated that lncRNA OTUD6B-AS1 was up-regulated in cisplatin-resistant cervical cancer cells, and knocking down OTUD6B-AS1 expression induced re-acquirement of chemosensitivity to cisplatin in cervical cancer cells. We also showed that OTUD6B-AS1 up-regulated the expression of CCND2 by sponging miR-206. Low miR-206 and high OTUD6B-AS1 expression were associated with significantly poorer overall survival. Taken together, these results suggest that OTUD6B-AS1-mediated down-regulation of miR-206 increases CCND2 expression, leading to cisplatin resistance. Modulation of these molecules may be a therapeutic approach for cisplatin-resistant cervical cancer.


2010 ◽  
Vol 1 (1) ◽  
pp. 54
Author(s):  
Rolando Enrique Canido ◽  
Geraldo Mota de Carvalho ◽  
Miriam Aparecida Barbosa Merighi ◽  
Alder Antônio Martins

RESUMOOs cânceres ginecológicos são responsáveis por alta morbidade e mortalidade de mulheres constituindo um grave problema de saúde pública no País. Este estudo teve como objetivo avaliar os resultados obtidos no Programa de Prevenção do Câncer de Colo de Útero e de Mama em Paranapanema, São Paulo, com vistas à sugestão de implantação de rotinas, condutas e normas que agilizassem esse atendimento, facilitando a coleta de exames, os encaminhamentos para resolubilidade das alterações apresentadas. Os resultados apontaram que o Município superou a meta estabelecida pela Direção Regional de Saúde, na campanha de prevenção de câncer ginecológico promovida pelo Ministério de Saúde de março a maio de 2002, a equipe mostrou-se bem treinada para o programa de prevenção do câncer ginecológico, os recursos físicos foram suficientes e o processo estava em conformidade com a recomendação do Ministério da Saúde, necessitando de algumas pequenas adaptações. Evidenciou-se que a ausência de um protocolo de atendimento dificulta um pouco a assistência e sua continuidade e poderá resultar no diagnóstico tardio do câncer. Palavras-chave: Saúde da mulher; Câncer ginecológico.ABSTRACTGynecological cancers are responsible for a dramatic morbidity and mortality rate among women, constituting a public health problem in the country. The goal of this study was to evaluate the results obtained in the Breast and Cervical Cancer Prevention Program at Paranapanema, Sao Paulo, suggesting the implantation of routines, conducts, and regulations to make this attendance faster, and to make examinations collection and the solution to alterations presented easier. The city accomplished the target set by the Health Regional Board in the campaign for gynecological cancer prevention promoted by the Health Ministry, from March to May, 2002. The staff proved well-trained for the Gynecological Cancer Prevention Program. Physical resources were sufficient and the process complied with the recommendation from the Health Ministry, yet in need of a few minor adaptations. The lack of an attendance protocol makes providing assistance and tracking changes a little difficult, in ways that may result in the late diagnosis of cancer. Keywords: Woman’s health; Gynecological cancer.RESUMENEl canceres ginecológicos son responsables por alta morbidad y mortalidad de mujeres constituyendo un problema de salud publica en el País. Este estudio tuve como objetivo avaluar los resultados obtenidos en el Programa de Prevención del Cáncer Cervical y del Pecho en Paranapanema, Sao Paulo, con vistas a la sugestión de implantación de rutinas, conductas y normas que agilizasen ese atendimiento, facilitando la coleta de examen, los encaminamientos para resolubilidad de las alteraciones presentadas. El municipio superó la meta establecida por la Dirección Regional de Salud, en la campaña de prevención del cáncer ginecológico promovida por el Ministerio de la Salud de marzo a mayo de 2002. El equipo de salud se mostró bien trenado para el programa de prevención del cáncer ginecológico. Los recursos físicos fueran suficientes y el proceso estaba en conformidad con la recomendación del Ministerio de la Salud, necesitando apenas algunas adaptaciones. La ausencia de uno protocolo de atendimiento dificultó un poco la asistencia y su continuidad y podrá resultar en el diagnóstico tardío del cáncer. Palabras clave: Salud de la mujer; Cáncer ginecológico. 


2022 ◽  
Vol 7 (1) ◽  
pp. 238146832110710
Author(s):  
Allison Portnoy ◽  
Mari Nygård ◽  
Lill Trogstad ◽  
Jane J. Kim ◽  
Emily A. Burger

Introduction. Delayed implementation of evidence-driven interventions has consequences that can be formally evaluated. In Norway, programs to prevent cervical cancer (CC)—screening and treatment of precancerous lesions and prophylactic vaccination against human papillomavirus (HPV) infection—have been implemented, but each encountered delays in policy implementation. To examine the effect of these delays, we project the outcomes that would have been achieved with timely implementation of two policy changes compared with the de facto delays in implementation (in Norway). Methods. We used a multimodeling approach that combined HPV transmission and cervical carcinogenesis to estimate the health outcomes and timeline for CC elimination associated with the implementation of two CC prevention policy decisions: a multicohort vaccination program of women up to age 26 years with bivalent vaccine in 2009 compared with actual “delayed” implementation in 2016, and a switch from cytology to primary HPV-based testing in 2015 compared with “delayed” rollout in 2020. Results. Timely implementation of two policy changes compared with current Norwegian prevention policy timeline could have averted approximately 970 additional cases (range of top 10 sets: 830–1060) and accelerated the CC elimination timeline by around 4 years (from 2039 to 2035). Conclusions. If delaying implementation of effective and cost-effective interventions is being considered, the decision-making process should include quantitative analyses on the effects of delays.


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