Ultrasound-Assisted Extraction of Total Flavonoids from Pteris cretica L.: Process Optimization, HPLC Analysis, and Evaluation of Antioxidant Activity

In the present work, the ultrasonic-assisted extraction (UAE) of total flavonoids (TF) from Pteris cretica L. was optimized by response surface methodology (RSM) on the basis of a single-factor experiment. The optimized UAE parameters were as follows: Ethanol concentration 56.74%, extraction time 45.94 min, extraction temperature 74.27 °C, and liquid/solid ratio 33.69 mL/g. Under the optimized conditions, the total flavonoids yield (TFY) was 4.71 ± 0.04%, which was higher than that obtained by heat reflux extraction (HRE). The extracts were further analyzed by HPLC, and five major flavonoids, including rutin, quercitrin, luteolin, apigenin, and luteolin-7-O-glucoside, were identified and quantified. Furthermore, the results of the antioxidant test showed that the TF extract obtained under optimized UAE conditions exhibited good 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS+•), nitric oxide radical (NO•) scavenging activities, and ferrous ion (Fe2+) chelating capacity, with IC50 values of 74.49, 82.92, 89.12, and 713.41 µg/mL, respectively. Results indicated that the UAE technique developed in this work was an efficient, rapid, and simple approach for the extraction of flavonoids with antioxidant activity from P. cretica.

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Response Surface Optimized Ultrasonic Assisted Extraction of Total Flavonoids from QingLi Cao and Antioxidant Activities

The Open Biotechnology Journal ◽

10.2174/1874070701509010134 ◽

2015 ◽

Vol 9 (1) ◽

pp. 134-142 ◽

Cited By ~ 2

Author(s):

Xudong Jiang ◽

YaoLing Liao ◽

GuiXi Lu ◽

Zhike Xiao

Keyword(s):

Response Surface ◽

Antioxidant Activities ◽

Reducing Power ◽

Total Flavonoids ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

An ultrasound-assisted extraction technique was used to extract the total flavonoids from QingLi Cao. The optimal conditions were ethanol concentration 59.20%, liquid-to-solid ratio 31.15 mL/g, extraction time 57.42 min and extraction temperature 58.57°C, which were determined using response surface methodology. The antioxidant activities including reducing power, ABTS+, DPPH, superoxide anion and hydroxyl radical were evaluated, which suggested significant antioxidant activities.

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PENGARUH KONSENTRASI ETANOL TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN RAMBUSA (Passiflora foetida L.) MENGGUNAKAN METODE ULTRASONIC ASSISTED EXTRACTION (UAE)

Jurnal Ilmu dan Teknologi Pangan (ITEPA) ◽

10.24843/itepa.2020.v09.i03.p05 ◽

2020 ◽

Vol 9 (3) ◽

pp. 291

Author(s):

I Made Ananta Divasti Guna ◽

I Nengah Kencana Putra ◽

AA. Sri Wiadnyani

Keyword(s):

Antioxidant Activity ◽

Ethanol Concentration ◽

Total Phenol ◽

Total Flavonoids ◽

Randomized Design ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Ic50 Values ◽

Effect On Yield ◽

Passiflora Foetida

This study was conducted to determine the effect of ethanol concentration on antioxidant activity and to obtain the highest antioxidant activity in rambusa leaves extract using the UAE method. The experimental design used in this study was a completely randomized design (CRD) with an ethanol concentration treatment consisting of five levels, namely 50%, 60%, 70%, 80%, and 90%. All treatments were repeated three times to obtain 15 experimental units. The data obtained were analyzed by the analysis of variance (ANOVA) and if the treatment had a significant effect, then followed by the Duncan test. The results showed that ethanol concentration had a significant effect on yield, total phenol, total flavonoids, total tannins, and antioxidant activity. The results showed that the treatment of 70% ethanol concentration was the best. The treatment produced the extract with a yield of 25,14%, total phenol of 40,94 mg GAE/g extract, total flavonoids of 38,95 mg QE/g, total tannin of 33,49 mg TAE/g, the antioxidant activity based on free radical inhibition percentage of 70,58 %, and IC50 values of 328.20 mg/L.

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Ultrasonic-Assisted Extraction and Natural Deep Eutectic Solvents Combination: A Green Strategy to Improve the Recovery of Phenolic Compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco

Antioxidants ◽

10.3390/antiox10040582 ◽

2021 ◽

Vol 10 (4) ◽

pp. 582

Author(s):

Inês Mansinhos ◽

Sandra Gonçalves ◽

Raquel Rodríguez-Solana ◽

José Luis Ordóñez-Díaz ◽

José Manuel Moreno-Rojas ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Deep Eutectic Solvents ◽

Industrial Applications ◽

Total Phenolic ◽

Ultrasound Assisted Extraction ◽

Phenolic Contents ◽

Assisted Extraction ◽

Natural Deep Eutectic Solvents ◽

Ultrasonic Assisted

The present study aimed at evaluating the effectiveness of different natural deep eutectic solvents (NADES) on the extraction of phenolic compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco, on the antioxidant activity, and acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (Tyr) inhibitory capacities. Ten different NADES were used in this research and compared with conventional solvents. Ultrasound-assisted extraction (UAE) for 60 min proved to be the best extraction condition, and proline:lactic acid (1:1) and choline chloride:urea (1:2) extracts showed the highest total phenolic contents (56.00 ± 0.77 mgGAE/gdw) and antioxidant activity [64.35 ± 1.74 mgTE/gdw and 72.13 ± 0.97 mgTE/gdw in 2.2-diphenyl-1-picrylhydrazyl (DPPH) and 2.2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods, respectively]. These extracts also exhibited enzymes inhibitory capacity particularly against Tyr and AChE. Even so, organic acid-based NADES showed to be the best extractants producing extracts with considerable ability to inhibit enzymes. Twenty-four phenolic compounds were identified by HPLC-HRMS, being rosmarinic acid, ferulic acid and salvianolic acid B the major compounds. The results confirmed that the combination of UAE and NADES provide an excellent alternative to organic solvents for sustainable and green extraction, and have huge potential for use in industrial applications involving the extraction of bioactive compounds from plants.

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Optimization of Ultrasonic-Assisted Extraction of Polysaccharides from Ottelia acuminata (Gagnep.) Dandy Using RSM

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.396-398.292 ◽

2011 ◽

Vol 396-398 ◽

pp. 292-296

Author(s):

Ai Shi Zhu

Keyword(s):

Processing Parameters ◽

Extraction Time ◽

Extraction Temperature ◽

Independent Variables ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasonic Technology ◽

Ultrasonic Assisted ◽

Experimental Yield ◽

Ultrasonic Assisted Extraction

Ultrasonic technology was applied to polysaccharides extraction from Ottelia acuminata (Gagnep.) Dandy and Response Surface Methodology (RSM) was used to optimize the effects of processing parameters on polysaccharides yields. Three independent variables such as liquid-solid ratio (ml/g, X1), extraction temperature (°C, X2) and extraction time (hour, X3) were investigated respectively. The statistical analysis indicated that the three variables and the quadratic of X1 and X3 had significant effects on the yields and followed by the significant interaction effects between the variables of X1 and X3, X2 and X3 (p<0.05). A mathematical model with high determination coefficient was gained. The optimal extraction conditions of polysaccharides were determined as follows: liquid-solid ratio 43 ml/g, extraction temperature 90 °C and extraction time 3.45 hours. Under these conditions, the experimental yield of polysaccharides was 107.44 mg/g, which was agreed closely with the predicted value 108.71 mg/g.

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Antioxidant Capacity and Antigenotoxic Effect of Hibiscus sabdariffa L. Extracts Obtained with Ultrasound-Assisted Extraction Process

Applied Sciences ◽

10.3390/app10020560 ◽

2020 ◽

Vol 10 (2) ◽

pp. 560

Author(s):

Gregorio Iván Peredo Pozos ◽

Mario Alberto Ruiz-López ◽

Juan Francisco Zamora Nátera ◽

Carlos Álvarez Moya ◽

Lucia Barrientos Ramírez ◽

...

Keyword(s):

Antioxidant Activity ◽

Human Lymphocytes ◽

Dry Weight ◽

Extraction Process ◽

Hibiscus Sabdariffa ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Antigenotoxic Effect

Hibiscus sabdariffa (Roselle) is in high demand worldwide due to its beneficial health properties owing to the polyphenols content, mainly in the flower calyx. The objective of this study was to find the best conditions (time and liquid: solid ratio) to extract polyphenols from Roselle using Ultrasound-Assisted Extraction (UAE) (40 kHz, 180 W), with ethanol how solvent; as well as determine the yield of phenols, anthocyanin, flavonoids, tannins, antioxidant activity (DPPH) and antigenotoxic effect (comet assay). A traditional solid-liquid extraction was applied as a reference. Extraction times of 40 and 60 min resulted in the highest polyphenols (13.019 mg GAE/g dry weight (dw)), flavonoids (4.981 CE/g dw), anthocyanins (1.855 mg Cya3GE/g dw), and tannins (0.745 CE/g dw) recoveries and an antioxidant activity (DPPH) of 74.58%. Extracts from white calyces contained similar amounts of phenols and flavonoids, but very little condensed tannins (0.049 CE/g dw) and practically no anthocyanins. Extracts from red and white calyces, showed antigenotoxic activity and repaired capacity of damage caused by mutagens in human lymphocytes.

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Optimization of Ultrasound-Assisted Extraction of Antioxidant Polyphenols from the Seed Coats of Red Sword Bean (Canavalia gladiate (Jacq.) DC.)

Antioxidants ◽

10.3390/antiox8070200 ◽

2019 ◽

Vol 8 (7) ◽

pp. 200 ◽

Cited By ~ 5

Author(s):

Yue Zhou ◽

Xiao-Yu Xu ◽

Ren-You Gan ◽

Jie Zheng ◽

Ya Li ◽

...

Keyword(s):

Extraction Method ◽

Natural Antioxidants ◽

Extraction Time ◽

Antioxidant Compounds ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Seed Coats

The seed coat of red sword bean (Canavalia gladiata (Jacq.) DC.) is rich in antioxidant polyphenols. It is often discarded as a byproduct with the consumption of red sword bean, since it is very thick and not consumed by people. The aim of this study was to develop an ultrasound-assisted extraction method to extract natural antioxidants from the seed coats. The extraction process was optimized by using response surface methodology. After the single-factor experiments, three key factors, including ethanol concentration, liquid/solid ratio, and extraction time, were selected and their interactions were studied using a central composite design. The optimal extraction condition was 60.2% hydroethanol, a liquid/solid ratio of 29.3 mL/g, an extraction time of 18.4 min, an extraction temperature of 50 °C, and ultrasound power of 400 W. Under the optimal conditions, antioxidant activity of the extract was 755.98 ± 10.23 μmol Trolox/g dry weight (DW), much higher than that from maceration (558.77 ± 14.42 μmol Trolox/g DW) or Soxhlet extraction (479.81 ± 12.75 μmol Trolox/g DW). In addition, the main antioxidant compounds in the extract were identified and quantified by high-performance liquid chromatography–diode array detection–tandem mass spectrometry (HPLC–DAD–MS/MS). The concentrations of digalloyl hexoside, methyl gallate, gallic acid, trigalloyl hexoside, and digallic acid were 15.30 ± 0.98, 8.85 ± 0.51, 8.76 ± 0.36, 4.27 ± 0.21, and 2.89 ± 0.13 mg/g DW. This study provides an efficient and green extraction method for the extraction of natural antioxidants from the bean coat of red sword bean. The extract of antioxidants might be added into functional foods or nutraceuticals with potential beneficial functions.

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Ultrasound and Microwave Assisted Extraction of Opuntia Fruit Peels Biocompounds: Optimization and Comparison Using RSM-CCD

Molecules ◽

10.3390/molecules24193618 ◽

2019 ◽

Vol 24 (19) ◽

pp. 3618 ◽

Cited By ~ 5

Author(s):

Bruno Melgar ◽

Maria Inês Dias ◽

Lillian Barros ◽

Isabel C.F.R. Ferreira ◽

Antonio D. Rodriguez-Lopez ◽

...

Keyword(s):

Antioxidant Activity ◽

Bioactive Compounds ◽

Bioactive Molecules ◽

Specific Response ◽

Microwave Assisted Extraction ◽

Ultrasound Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction ◽

Ic50 Values ◽

Effective Models

Ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) of bioactive compounds, peels from Opuntia engelmannii cultivar (cv.) Valencia were optimized by response surface methodology. Randomized extraction runs were performed for each of the technologies employed in order to build effective models with maximum (bioactive molecules content and yield) and minimum (antioxidant activity) responses. A 5-level, 4-factor central composite design was used to obtain target responses as a function of extraction time (t), solid to liquid ratio (S/L), methanol concentration (metOH), and temperature (T). Specific response optimization for each technology was analyzed, discussed, and general optimization from all the responses together was also gather. The optimum values for each factor were: t = 2.5 and 1.4 min, S/L = 5 and 5 g/L, metOH = 34.6 and 0% of methanol and T = 30 and 36.6 °C, achieving maximum responses of 201.6 and 132.9 mg of betalains/g, 13.9 and 8.0 mg of phenolic acids/g, 2.4 and 1.5 mg of flavonoids/g, 71.8% and 79.1% of extractable solid and IC50 values for the antioxidant activity of 2.9 and 3.6, for UAE and MAE, respectively. The present study suggested UAE as the best extraction system, in order to maximize recovery of bioactive compounds with a high antioxidant activity.

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Ultrasonic assisted extraction to obtain bioactive, antioxidant and antimicrobial compounds from marcela

Ciência Rural ◽

10.1590/0103-8478cr20170772 ◽

2018 ◽

Vol 48 (6) ◽

Cited By ~ 2

Author(s):

Caroline Pagnossim Boeira ◽

Natiéli Piovesan ◽

Marcela Bromberger Soquetta ◽

Déborah Cristina Barcelos Flores ◽

Bruna Nichelle Lucas ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Bioactive Compounds ◽

Extraction Method ◽

Ultrasonic Method ◽

Ultrasound Assisted Extraction ◽

Ultrasound Method ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Conventional Extraction

ABSTRACT: The aim of this study was to evaluate the effect of extraction conditions on bioactive compounds, as well as on antioxidant activity, and the antimicrobial activity of the extracts with the highest antioxidant characteristics. The extracts were obtained by conventional method and ultrasound-assisted extraction at various temperatures (20°C, 40°C and 60°C). Total phenolics, total flavonoids, antimicrobial activity and antioxidant activity were quantified by the methods of DPPH, FRAP, and ORAC, respectively. The conventional extraction method and ultrasound method influenced the phenolic content at all the tested temperatures. Flavonoids were not influenced by extraction methods. The antioxidant activity (DPPH) was highest in the ultrasonic method at temperatures of 40°C and 60°C; however, in the case of the FRAP method the best results were for the conventional extraction method. The conventional and ultrasonic methods did not influence the IC50 at temperatures of 20°C and 40°C, but using ORAC the antioxidant activity was influenced by the methods at all temperatures. The extract obtained at 60°C by the ultrasound method had high antimicrobial action in relation to the strains of Salmonella sp., Escherichia coli, and Staphylococcus aureus. Extraction ultrasonic-assisted can be considered adequate to obtain extracts of marcela, which are rich in bioactive compounds with high antioxidant activity.

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Antioxidant Activity of Extract from Ultrasonic-Assisted Extraction of Durian Peels

Journal of Applied Food Technology ◽

10.17728/jaft.3309 ◽

2018 ◽

Vol 5 (2) ◽

Cited By ~ 1

Author(s):

Bambang Kunarto ◽

Ely Yuniarti Sani

Keyword(s):

Antioxidant Activity ◽

Total Phenolics ◽

Extraction Process ◽

Extraction Time ◽

Total Flavonoids ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Total Flavonoids Content ◽

Phenolics Content ◽

Ultrasonic Assisted Extraction

The increase in durian production results in the accumulation of durian peel waste. The bioactive component of durian peel has the potential to be used as an antioxidant. Thus, there is a need to carry out an extraction process to obtain bioactive compounds from durian peel. However, conventional extraction methods cause damage to phenolic compounds due to oxidation, hydrolysis and ionization reactions during the extraction process. Therefore, durian peel extraction was carried out using ultrasonic assisted extraction method (UAE) in this study. The purpose of this study was to investigate the effect of varying ratios of durian peel to ethanol solvents and extraction time on the yield, total phenolics content, total flavonoids content and antioxidant activity. The results of the research data were analyzed using a two-factor completely randomized design, which included variations of the ratio of durian peel to ethanol solvents and extraction time The Duncan’s New Multiple Range Test (DNMRT) was carried out as a follow up test to determine the differences in each treatment at a significance level of 0.05. The results showed that the best treatment for peel extraction using ultrasonic assisted extraction was a 1: 9 ratio of durian peel to ethanol at an extraction time of 20 minutes. The extraction of durian peel under this condition gave the highest yield of 12.77 ± 0.16%, antioxidant activity (IC50) of 38.33 ± 0.12 ppm, total phenolic content of 63.30 ± 0.08 mgGAE / g and total flavonoids content of 47.53 ± 0.48 mgQE / g. In addition, total phenolics content and total flavonoid content showed a strong correlation to the antioxidant activity of durian peel extract.

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Optimization of Ultrasound-Assisted Extraction on Antioxidative Activity of Malus toringoides Using Response Surface Methodology

Processes ◽

10.3390/pr7050270 ◽

2019 ◽

Vol 7 (5) ◽

pp. 270 ◽

Cited By ~ 1

Author(s):

Qiang Ye ◽

Li Guo ◽

Hongmei Liu ◽

Yushi Liu ◽

Cunyan Zhang ◽

...

Keyword(s):

Oxidative Stress ◽

Response Surface Methodology ◽

Response Surface ◽

Umbilical Vein ◽

Extraction Temperature ◽

Least Squares Regression ◽

Protective Activity ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction

Ultrasonic-assisted extraction (UAE) was optimized using response surface methodology (RSM) to maintain the cyto-protective activity of M.toringoides against oxidative stress. The optimal conditions for UAE were a 58 mL/g liquid-solid ratio, a 38 °C extraction temperature, an 85% solvent concentration, and a 19-min extraction time, which resulted in a protection rate of 54.57% against hydrogen peroxide-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). These results were comparable to the predicted value of 53.75%. The extracts showed excellent antioxidant activity, and phlorizin was detected in the dried leaves of Malus.toringoides. The highest yield of phlorizin (101.239 mg/g) was also obtained using these conditions. Taken together, these results showed that the method successfully integrated RSM and partial least squares regression methods to optimize M.toringoides extraction to yield the highest cyto-protective activity and effectively increase the yield of phlorizin from M.toringoides.

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