scholarly journals Peppermint Essential Oil-Doped Hydroxyapatite Nanoparticles with Antimicrobial Properties

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2169 ◽  
Author(s):  
Monica Luminita Badea ◽  
Simona Liliana Iconaru ◽  
Andreea Groza ◽  
Mariana Carmen Chifiriuc ◽  
Mircea Beuran ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Candida Parapsilosis ◽  
Antimicrobial Properties ◽  
Gram Negative Bacteria ◽  
Hydroxyapatite Nanoparticles ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Ellipsoidal Shape ◽  
Ir Bands

This study aimed at developing an antimicrobial material based on hydroxyapatite (HAp) and peppermint essential oil (P-EO) in order to stimulate the antimicrobial activity of hydroxyapatite. The molecular spectral features and morphology of the P-EO, HAp and hydroxyapatite coated with peppermint essential oil (HAp-P) were analyzed using Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The coating of the HAp with the P-EO did not affect the ellipsoidal shape of the nanoparticles. The overlapping of IR bands of P-EO and HAp in the HAp-P spectrum determined the formation of the broad molecular bands that were observed in the spectral regions of 400–1000 cm−1 and 1000–1200 cm−1. The antibacterial activity of the P-EO, HAp and HAp-P were also tested against different Gram-positive bacteria (methicillin-resistant Staphylococcus aureus (MRSA) 388, S. aureus ATCC 25923, S. aureus ATCC 6538, E. faecium DSM 13590), Gram-negative bacteria (Escherichia coli ATCC 25922, E. coli C5, P. aeruginosa ATCC 27853, P. aeruginosa ATCC 9027) and a fungal strain of Candida parapsilosis. The results of the present study revealed that the antimicrobial activity of HAp-P increased significantly over that of HAp.

Disinfection of Fruits with Activated Water

2019 ◽  
Vol 10 ◽  
pp. 1864-1872
Author(s):  
Prof. Teodora P. Popova
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Solutions ◽  
Antimicrobial Properties ◽  
The Other ◽  
Gram Negative Bacteria ◽  
High Antimicrobial Activity ◽  
Gram Negative ◽  
E Coli ◽  
Number Of Microorganisms ◽  
Lower Effect

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.

scholarly journals Essential Oil Composition and Antimicrobial Activity of Vismia macrophylla Leaves and Fruits Collected in Táchira-Venezuela

2015 ◽  
Vol 10 (2) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Alexis Buitrago ◽  
Janne Rojas ◽  
Luis Rojas ◽  
Judith Velasco ◽  
Antonio Morales ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oil Composition ◽  
Gram Negative Bacteria ◽  
Oil Composition ◽  
Gram Positive ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Germacrene D ◽  
Leaf Oil ◽  
Fruit Oil

Hydrodistillation of Vismia macrophylla Kunth (Hypericaceae) leaves (L) and fruits (F) yielded 1.3%, v/w, and 5.6%, v/w, of essential oil, respectively. GC and GC-MS analyses showed the presence of twenty-four (96.4%, L) and thirty-one (96.6%, F) components, respectively. Major compounds identified in the leaf oil were γ-bisabolene (44.4%) and β-bisabolol (14.9%), while those in the fruit oil were germacrene-D (12.1%), δ-cadinene (10.7%) and γ-bisabolene (22.3 %). Oil obtained from the fruits of V. macrophylla showed antibacterial activity against Gram-positive (S. aureus ATCC 25923 and E. faecalis ATCC 29212) as well as Gram-negative bacteria (E. coli ATCC 25922), with MIC values ranging from 150 μL/mL to 740 μLmL. Oil obtained from leaves were active only on the Gram-positive bacteria S. aureus (100 μLmL) and E. faecalis (500 μLmL), but also showed antiyeast activity against Candida albicans CDC-B385 and C krusei ATCC 6258 (600 μL/mL, each).

Chemical Composition and Antimicrobial Activity of Teucrium Capitatum L. Subsp. Lusitanicum (Schreb.) T. Navarro & Rosua Essential Oil

2020 ◽  
Author(s):  
Haïfa Debbabi ◽  
Ridha El Mokni ◽  
Ibrahim Jlassi ◽  
Rajesh K Joshi ◽  
Saoussen Hammami
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Significant Activity ◽  
Bacterial Strains ◽  
Gram Positive Bacteria ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D

Abstract Chemical composition and antimicrobial activity of Teucrium capitatum L. subsp. lusitanicum essential oil was investigated for the first time in the present study. Qualitative and quantitative analyses of the chemical composition by gas chromatography and mass spectrometry (GC–FID and GC–MS) revealed the presence of 60 compounds representing 97.6% of the whole constituents. The main compounds were germacrene D (47.1%), spathulenol (5.8%), α-selinene (5.3%), germacrene A (2.9%), δ-cadinene (2.8%) and cubenol (2.7%). In vitro, the antimicrobial activity was investigated against five bacterial strains along with the yeast Candida albicans using broth microdilution assay. T. capitatum subsp. lusitanicum essential oil showed significant activity against the gram-positive bacteria Staphylococcus aureus (MIC = MBC = 78 μg mL−1), Bacillus subtilis (MIC = MBC = 156 μg mL−1) and the yeast C. albicans (MIC = MFC = 156 μg mL−1). The great potential of antimicrobial effects is most likely due to the very high percentage of sesquiterpene hydrocarbons particularly to germacrene D, for which the antimicrobial properties have been previously reported.

scholarly journals Composition and Antimicrobial Analysis of the Essential Oil of Litsea Laevigata Nees. (Lauraceae)

2008 ◽  
Vol 3 (7) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Arif M Muhammed ◽  
Raj M Subbu ◽  
Leopold Jirovetz ◽  
Shafi P Mohamed
Keyword(s):  
Antimicrobial Activity ◽  
Aspergillus Niger ◽  
Essential Oil ◽  
Western Ghats ◽  
Klebsiella Aerogenes ◽  
Gram Negative Bacteria ◽  
Proteus Vulgaris ◽  
High Antimicrobial Activity ◽  
Gram Positive ◽  
Gram Positive Bacteria

The essential oil of the berries of Litsea laevigata Nees., growing wild in Western Ghats, Kerala, India, was obtained by hydrodistillation and was fractionated by passage through a column using n-pentane and diethyl ether as eluents. The essential oil and its fractions were analysed by GC and GC-MS. Twenty seven compounds representing 99.2% of the original oil were identified. The major components are the terpene hydrocarbons trans-α-bergamotene(26.7%), α-pinene (25%) and β-pinene (8.2%). The antimicrobial activity of the essential oil and its fractions against four Gram-positive and four Gram-negative bacteria ( Staphylococcus aureus, Bacillus subtilis, Streptococcus faecalis, Staphylococcus albus, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella aerogenes), as well as two fungi ( Candida albicans, Aspergillus niger) was studied. The bioassay showed that the oil exhibited moderate to high antimicrobial activity, being very active against gram-positive bacteria, such as Streptococcus albus, and the fungus Aspergillus niger.

scholarly journals Chemical Composition and in vitro Antimicrobial Activity of the Essential Oil of the Flowers of Tridax procumbens

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Rajesh K. Joshi ◽  
Vijaylaxmi Badakar
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Flame Ionization ◽  
Tridax Procumbens

The essential oil of the flowers of Tridax procumbens L. was obtained by hydro-distillation and analyzed by gas chromatography equipped with a flame ionization detector (GC-FID) and gas chromatography coupled with mass spectrometry (GC/MS). Twenty-six compounds were identified, which comprised 90.6% of the total constituents. The most abundant compound was ( Z)-falcarinol (25.9%), followed by α-selinene (15.3%), limonene (8.3%) and zerumbone (4.3%). Antimicrobial activity was tested against six Gram-positive and eight Gram-negative bacteria, and three fungi. The oil was active against the tested Gram-positive bacteria at a concentration range of 0.14 ± 0.03 - 0.57±0.05 mg/mL, while 0.67 ± 0.12 - 4.58 ± 0.41 mg/mL was effective against the studied Gram-negative bacteria. Remarkable antifungal activity was found against the tested fungi at a concentration range of 0.06 ± 0.008 - 0.10 ± 0.01 mg/mL.

scholarly journals Essential oils of Thymus pulegioides and Thymus glabrescens from Romania: Chemical composition and antimicrobial activity

2010 ◽  
Vol 75 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Mariana Pavel ◽  
Mihailo Ristic ◽  
Tatjana Stevic
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Micrococcus Luteus ◽  
Antimicrobial Properties ◽  
Herbal Drug ◽  
Qualitative And Quantitative Analysis ◽  
Gram Positive Bacteria ◽  
Main Components

The aim of this work was to analyse the chemical composition and antimicrobial properties of essential oils isolated from two wild-growing species of thyme (Thymus pulegioides L. and T. glabrescens Willd.) originating from different locations in Romania. The yield of essential oil was determined according to European Pharmacopoeia standards. Qualitative and quantitative analysis of the oils was performed using GC and GC/MS. The antimicrobial activity was tested by the microdilution technique against Gram-negative and Gram-positive bacteria (Escherichia coli, Salmonella typhimurium, S. enteritisdis, Enterobacter cloacae, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, S. epidermidis, Streptococcus faecalis, Bacillus subtilis, Micrococcus luteus, M. flavus and Listeria monocytogenes) and human pathogen yeast Candida albicans. The essential oil of Thymus pulegioides was obtained in a yield of 0.7-1 % (v/d.w. herbal drug) and the main components were carvacrol (50.5-62.6 %), ?-terpinene (9.8-9.9 %) and p-cymene (5.8-7.1 %). The essential oil of T. glabrescens was obtained in a yield of 0.7 (v/d.w. herbal drug) and the main components were geraniol (55.5 %), neryl acetate (11.1 %) and ?-bisabolene (6.7 %). The essential oils inhibited microbial growth at concentrations of 10.8-27 ?l/ml.

scholarly journals Evaluation of Antimicrobial Activity of Acacia etbaica Water Extract Leafs against Some Pathogenic Microorganisms

2021 ◽  
Vol 2 (11) ◽  
pp. 1132-1135
Author(s):  
Abdulmageed B Abdullah ◽  
Abdulbaki Al-zaemey ◽  
Rasheed Hasan Mudhesh Al-Husami ◽  
Mofeed Al-Nowihi
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Water Extract ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Inhibition Zones

The antimicrobial activity about water extract of Acacia etbaica was examined by using agar well diffusion methods against five gram-positive and negative bacteria[Staphylococcus aureus (ATCC653-8), Pseudomonas aeruginosa MTCC2453, Bacillus cereus (ATCC6633), Escherichia coli MTCC739, and one local isolate (Staphylococcus epidermides)] in addition to Candida albicans (ATCC2019). this results designated that the water extract of Acacia etbaica possess antimicrobial efficacy against all tested microorganisms either (gram-positive and negative bacteria) or fungi (Candida albicans).Whereas the gram-positive bacteria (S. aureus, S. epidermides and B. cereus) with inhibition zones (21, 19.5 and 16.5) respectively was more sensitive than gram-negative bacteria (P. aeruginosa and E. coli) and C. albicans with inhibition zones 16 and 14.5 respectively. The antimicrobial effect was directly proportional with concentration of water extract where the highest inhibition zone at higher concentration 30% equal 21mm against S.aureus and the lower inhibition zone at lower concentration 5% equal 10mm against C. albicans...

scholarly journals Study on Antibacterial Activity of Common Spices

2012 ◽  
Vol 12 ◽  
pp. 312-317 ◽  
Author(s):  
Dinesh Maharjan ◽  
Anjana Singh ◽  
Binod Lekhak ◽  
Shaila Basnyat ◽  
Lekhnath S Gautam
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Minimal Bactericidal Concentration ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Methanol Extracts ◽  
Gram Positive Bacteria ◽  
Medicinal Value ◽  
Common Spices

Spices have been shown to possess medicinal value, in particular, antimicrobial activity. They are used as householdmedicines as well as preservatives of food materials. This study compares the sensitivity of some human pathogenicbacteria to various spice extracts viz. essential oils, acetone and methanol extracts by agar well diffusion method.Of the different spices tested clove, ajowan and cinnamon were found to possess relatively higher antimicrobialactivities. Essential oil of cinnamon showed broad spectrum of inhibition against all tested bacteria while essentialoil of ajowan and clove inhibited 90% and 70% bacteria respectively. Acetone and methanol extracts of cloveshowed better antibacterial activity among the spices. The MBC value ranged from 0.39 to 25mg/ml. The lowestMBC (minimal bactericidal concentration) value was given by essential oil of cinnamon against E.coli, S. aureusand S. Typhi. Gram positive bacteria were found to be more sensitive to spices than Gram negative bacteria. Spices might have a great potential to be used as antimicrobial agents.DOI: http://dx.doi.org/10.3126/njst.v12i0.6518 Nepal Journal of Science and Technology 12 (2011) 312-317 

scholarly journals Comparison of Antimicrobial Activity of Chitosan Nanoparticles against Bacteria and Fungi

Coatings ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 769
Author(s):  
Yage Xing ◽  
Xiaomin Wang ◽  
Xunlian Guo ◽  
Ping Yang ◽  
Jinze Yu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Fungal Pathogens ◽  
Chitosan Nanoparticles ◽  
Gram Negative Bacteria ◽  
Damping Off ◽  
Absorption Intensity ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Antifungal Effects ◽  
Bacteria And Fungi

Chitosan nanoparticles (CSNPs) have attracted wide interest; however, there has been no substantial information about a direct comparison of the antimicrobial activity of CSNPs on bacteria and fungi. Thus, in this study, simple, economically feasible CSNPs were synthesized and assessed for their antimicrobial activity. This investigation indicated that the coordination inducing effect of CSNPs could dissociate the tryptophan (Trp) and tyrosine (Tyr) residue groups on the peptide chain of the bovine serum albumin (BSA) molecule, thereby increasing the absorption intensity. The growth of E. coli and S. aureus could be completely inhibited when the concentration of CSNPs in the solution was higher than 0.6 mg/mL. The CSNPs showed more potent antibacterial activity against Gram-negative bacteria (E. coli) than against Gram-positive bacteria (S. aureus). In addition, the CSNPs were effective at initiating cellular leakage of fungal mycelia and damping off fungal pathogens, and their antifungal effects were stronger on P. steckii than on A. oryzae. Furthermore, the antimicrobial activity of the CSNPs was found to be more effective against bacteria than against fungi. This study thus ascertained the antimicrobial activity of synthesized CSNPs against different microorganisms, as well as their different degrees of inhibition.

scholarly journals Antimicrobial Activity of Selected Essential Oils against Selected Pathogenic Bacteria: In Vitro Study

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 546
Author(s):  
Nikola Puvača ◽  
Jovana Milenković ◽  
Tamara Galonja Galonja Coghill ◽  
Vojislava Bursić ◽  
Aleksandra Petrović ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Tea Tree ◽  
E Coli

The worldwide problem of infectious diseases has appeared in recent years, and antimicrobial agents are crucial in reducing disease emergence. Nevertheless, the development and distribution of multidrug-resistant (MDR) strains in pathogenic bacteria, such as Escherichia coli, Staphylococcus aureus, Salmonella Typhi and Citrobacter koseri, has become a major society health hazard. Essential oils could serve as a promising tool as a natural drug in fighting the problem with these bacteria. The current study aimed to investigate the antimicrobial effectiveness of tea tree (Melaleuca alternifolia (Maiden and Betche) Cheel), rosemary (Rosmarinus officinalis L.), eucalyptus (Eucalyptus obliqua L’Hér.), and lavender (Lavandula angustifolia Mill) essential oils. The antimicrobial properties of essential oils were screened against four pathogenic bacteria, E. coli, S. aureus, S. Tyhpi, and C. koseri, and two reference bacterial strains, while for the testing, the agar well diffusion method was used. Gas chromatography (GC) and gas chromatography–mass spectrometric (GC–MSD) analyses were performed on essential oils. The obtained results showed that M. alternifolia essential oil is the richest in terpinen-4-ol, R. officinalis and E. oblique essential oils in 1,8-cineole, and L. angustifolia essential oil in α-terpinyl acetate. In addition, the main bioactive compounds present in the essential oil of tea tree are rich in α-pinene (18.38%), limonene (7.55%) and γ-terpinene (14.01%). The essential oil of rosemary is rich in α-pinene (8.38%) and limonene (11.86%); eucalyptus essential oil has significant concentrations of α-pinene (12.60%), p-cymene (3.24%), limonene (3.87%), and γ-terpinene (7.37%), while the essential oil of lavender is rich in linalool (10.71%), linalool acetate (9.60%), α-terpinyl acetate (10.93%), and carbitol (13.05%) bioactive compounds, respectively. The obtained results from the in vitro study revealed that most of the essential oils exhibited antimicrobial properties. Among the tested essential oils, tea tree was discovered to demonstrate the strongest antimicrobial activity. The recorded MIC of S. Typhi was 6.2 mg/mL, 3.4 mg/mL of C. koseri, 3.1 mg/mL of E. coli, and 2.7 mg/mL of E. Coli ATCC 25922, compared to M. alternifolia. Similarly, only S. aureus ATCC 25923 showed antimicrobial activity towards R. officinalis (1.4 mg/mL), E. oblique (2.9 mg/mL), and L. angustifolia (2.1 mg/mL). Based on the obtained results, it is possible to conclude that tea tree essential oil might be used as an ecological antimicrobial in treating infectious diseases caused by the tested pathogens.

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