scholarly journals Microwave-Assisted Brine Extraction for Enhancement of the Quantity and Quality of Lipid Production from Microalgae Nannochloropsis sp.

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3581 ◽  
Author(s):  
Zghaibi ◽  
Omar ◽  
Kamal ◽  
Biak ◽  
Harun
Keyword(s):  
Fatty Acids ◽  
Low Cost ◽  
Methyl Esters ◽  
Lipid Production ◽  
Lipid Extraction ◽  
Soxhlet Extraction ◽  
Solid Loading ◽  
Omega 3 ◽  
Microwave Assisted

Toward attaining a sustainability and eco-friendly process, a green and low-cost solvent—brine (NaCl solution) is proposed, as microwave-assisted extraction (MAE) technique solvent to extract lipids from microalgae Nannochloropsis sp. The effect of NaCl concentration on the quantity and quality of the extracted lipid was assessed, while MAE parameters were optimized using response surface methodology (RSM). The content of fatty acid methyl esters (FAMEs) in the lipid was analyzed by using a gas chromatography—flame ionization detector (GC/FID). The highest lipid yield (16.1%) was obtained using 10% (w/v) brine at optimum extraction parameters of 5% (w/v) solid loading, 100 °C, and 30 min. The lipid extraction yield via optimized MAE-brine technique was thrice better than that Soxhlet extraction did and only 2% less than Bligh and Dyer (B&D) lipid extraction, which utilized harmful solvents. The proposed MAE-brine technique offered better quality lipids containing the highest amount of polyunsaturated fatty acids (PUFA) (44.5%) and omega-3 fatty acids (FAs) (43%). Hence, the MAE-brine solvent technique appears to be a promising extraction method for cheaper, greener, and faster extraction of a high-quality lipid for specialty food applications.

scholarly journals Chemical, Nutritional and Antioxidant Characteristics of Different Food Seeds

2020 ◽  
Vol 10 (5) ◽  
pp. 1589 ◽  
Author(s):  
Lacrimioara Senila ◽  
Emilia Neag ◽  
Oana Cadar ◽  
Melinda Haydee Kovacs ◽  
Anca Becze ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Protein Content ◽  
Methyl Esters ◽  
Lipid Extraction ◽  
Omega 3 ◽  
Hemp Seed ◽  
Chromatography Analysis ◽  
Total Fatty Acids ◽  
Antioxidant Characteristics

The objective of this study was to determine the chemical composition of five different food seeds (sunflower, poppy, hemp, flax and sesame) regarding fatty acid, mineral (Fe, Cu, Zn, Na, Mg, K, Ca, Al) and protein content. In addition, the total antioxidant capacity of the seeds was evaluated using the photochemiluminescent assay. The food seeds were subjected to lipid extraction and converted into fatty acid methyl esters before the gas chromatography analysis. In all food seeds, the saturated (SFAs), monounsaturated (MUFAs) and polyunsaturated fatty acids (PUFAs) were identified, respectively. PUFAs were the most abundant fatty acids (61.2% ± 0.07% and 84.8% ± 0.08% of total fatty acids), with the highest content in flax and hemp seed oil. Also, high amounts of omega-3 from PUFAs were determined in flax and hempseed oil. Based on the obtained results the sunflower, sesame and poppy seeds are good sources of omega-6, while flax and hemp seeds are good sources of omega-3. All samples are rich in minerals (Na, K, Ca, Mg) and have more than 20% protein content.

scholarly journals Comparison of Ex-Situ and In-Situ Transesterification for the Production of Microbial Biodiesel

2021 ◽  
Vol 16 (4) ◽  
pp. 733-743
Author(s):  
Alia Tasnim Hazmi ◽  
Farah B. Ahmad ◽  
Ahdyat Zain Athoillah ◽  
Ahmad Tariq Jameel
Keyword(s):  
Reaction Time ◽  
Fungal Biomass ◽  
Methyl Esters ◽  
Lipid Production ◽  
Lipid Extraction ◽  
Soxhlet Extraction ◽  
Ex Situ ◽  
Catalyst Loading ◽  
In Situ Transesterification

Microbial biodiesel is converted from microbial lipids via transesterification process. Most microbial biodiesel studies are focusing on the use of microalgal lipids as feedstock. Apart from using microalgae for lipid biosynthesis, lipids can also be extracted from other oleaginous microorganisms like fungi and yeast. However, there are gaps in the studies of lipid production from filamentous fungi, especially in-situ transesterification process. The aim of this project is to compare in-situ with the ex-situ transesterification of fungal biomass from Aspergillus oryzae. In ex-situ transesterification, two methods of lipid extraction, the Soxhlet extraction and the Bligh and Dyer extraction, were performed. For in-situ transesterification, two methods using different catalysts were investigated. Base-catalyzed in-situ transesterification of fungal biomass resulted on the highest Fatty Acid Methyl Esters (FAME) yield. The base-catalyzed in-situ transesterification was further optimized via Central Composite Design (CCD) of Response Surface Methodology (RSM). The parameters investigated were the catalyst loading, methanol to biomass ratio and reaction time. The optimization showed that the highest FAME yield was at 25.1% (w/w) with 10 minutes reaction time, 5% catalyst and 360:1 of the ratio of the methanol to biomass. Based on Analysis of Variance (ANOVA), the model was found to be significant according to the value of “Prob >F” of 0.0028. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0). 

scholarly journals Effect of dietary supplementation with omega-3 and -6 on fresh and frozen/thawed sperm quality of dogs

2017 ◽  
Vol 38 (5) ◽  
pp. 3069 ◽  
Author(s):  
Ana Carolina Rodrigues ◽  
Camila Montanari Ruiz ◽  
Carla Daniela Dan De Nardo ◽  
Gabriele Barros Mothé ◽  
Fabiano Martinez Rossi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Dietary Supplementation ◽  
Reproductive Efficiency ◽  
Omega 3 ◽  
Oral Supplementation ◽  
Animal Reproduction ◽  
Fresh Semen

For years, fatty acids have been recommended as a dietary supplement to improve canine hair. For animal reproduction, supplementation with omegas has been used to increase the reproductive efficiency and conception rate, but few studies have been conducted in dogs. The aim of this study was to evaluate the effects of daily dietary supplementation with omega-3 and -6 on the quality of fresh and frozen/thawed semen in canines. Semen was collected from seven dogs and evaluated for sperm motility, vigor, concentration, and morphology. The 17-week study included 119 ejaculates and was divided according to oral supplementation with omega-3 and -6: M1 (1st-5th week) or pre-supplementation; M2 (6th-9th week) and M3 (10th-13th week) or during supplementation; and M4 (14th-17th week) or post-supplementation. After analysis, the semen was frozen and then revaluated both immediately and 30 minutes (at 37° C) after thawing. Supplementation with omegas increased sperm motility, vigor, and concentration; however, supplementation had no influence on semen freezability. In addition, there was no improvement in sperm motility after supplementation when the thawed cells were maintained at 37° C for 30 minutes. We concluded that dietary supplementation with omega-3 and -6 for 4 to 8 weeks can improve the quality of fresh semen, although it has no effect on the freezability of canine semen.

scholarly journals Associations of Erythrocyte Polyunsaturated Fatty Acids with Inflammation and Quality of Life in Post-Menopausal Women with Obesity Completing a Pilot Dietary Intervention

Nutrients ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1589
Author(s):  
Kylie M. Johnson ◽  
Kellie R. Weinhold ◽  
Rebecca Andridge ◽  
Kristen Arnold ◽  
Panchita P. Chu ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Dietary Intervention ◽  
Short Form ◽  
Omega 3 ◽  
Markers Of Inflammation ◽  
Menopausal Women ◽  
Post Menopausal

Study objectives were to determine if erythrocyte omega-3 polyunsaturated fatty acids (n-3 PUFAs) increased in women participating in a dietary intervention that reduced inflammation and body weight and examine PUFA associations with markers of inflammation and quality of life (QOL). An experimental pre-post test, single group design was used. Fifteen post-menopausal women with obesity were enrolled in a 12-week pilot intervention focusing on lowering added sugars and increasing fiber and fish rich in n-3 PUFAs. Measurements included fasting blood samples, anthropometric, lifestyle and dietary data collected at baseline, end of intervention (Week 12) and follow-up (Week 24). Primary outcomes were change in erythrocyte PUFAs and associations between erythrocyte PUFAs, QOL (Short Form 12), and inflammatory markers (interleukin-6, tumor necrosis factor-α-receptor 2, and high sensitivity C-reactive protein (CRP)). Fourteen women completed all intervention visits. Mean erythrocyte docosahexaenoic acid and arachidonic acid (AA) increased at Week 12 and Week 24 (p < 0.001 for both), while eicosapentaenoic acid increased at Week 24 (p < 0.01). After adjustment for percent weight change, week 12 QOL related to physical function was significantly associated with erythrocyte linoleic acid (p < 0.05) and trended toward significant association with EPA (p = 0.051); week 24 CRP was directly associated with erythrocyte AA (p < 0.05). Erythrocyte n-3 PUFAs were not associated with inflammation.

IMPROVEMENT OF THE BIOLOGICAL EFFECTIVENESS OF FAT FROM THE LIVER OF THE BLACK SEA SHARK-KATRAN

2021 ◽  
Author(s):  
Е.Е. ИВАНОВА ◽  
Е.А. ДУБИНЕЦ ◽  
А.Л. БОЧАРОВА-ЛЕСКИНА
Keyword(s):  
Fatty Acids ◽  
Iodine Number ◽  
Black Sea ◽  
Liquid Fraction ◽  
Saturated Fatty Acids ◽  
Biologically Active ◽  
The Black Sea ◽  
Omega 3 ◽  
Biological Effectiveness

Для стабилизации показателя биологической эффективности исследована возможность повышения количества полиненасыщенных жирных кислот (ПНЖК) за счет снижения количества насыщенных жирных кислот (НЖК) в жирнокислотном составе жира из печени черноморской акулы-катрана в летний период вылова (с мая по октябрь). С целью повышения содержания омега-3 ПНЖК в продукте применен способ винтеризации. Кристаллизацию жира из печени акулы-катрана проводили в специальном охладителе при температуре от 0 до –5°С. Во время охлаждения жир медленно перемешивали (скорость вращения мешалки 20–25 об/мин) для равномерного охлаждения по всему объему. Полная кристаллизация НЖК при этих условиях проходила за 5–6 ч. Охлажденный жир немедленно направляли на осадительную центрифугу. В качестве параметров оптимизации процесса были выбраны: качество жира – прозрачность при температуре 15°С и степень ненасыщенности – йодное число жира. Установлено, что факторами, существенно влияющими на йодное число и органолептические показатели жира, являются: температура охлажденного жира, продолжительность центрифугирования, число оборотов барабана центрифуги. Для моделирования значений йодного числа и органолептического показателя в области изменения указанных факторов применена схема полного факторного двухуровневого эксперимента, в результате которого определены натуральные значения факторов, отвечающие наилучшему значению показателя качества жира: температура охлаждения жира –4°С, продолжительность центрифугирования 30 мин, частота вращения барабана центрифуги 6000 об/мин. Общая ненасыщенность жидкой фракции возросла с 122,2–138,0 до 131,0–145,0% йодного числа жира. Полученный очищенный жир был прозрачен, имел светло-желтый цвет, низкие значения кислотного и перекисного чисел. Содержание ПНЖК в полученном продукте увеличилось на 10–30%. Содержание неомыляемых веществ в нем, в том числе биологически активных веществ алкилглицеролов, при этом не снизилось. Биологическая эффективность жира увеличилась с 0,6 до 0,9. To stabilize the increased biological effectiveness the possibility of increasing the amount of polyunsaturated fatty acids (PUFA) by reducing the amount of saturated fatty acids (SFA) in the fatty acid composition of fat from the liver of the black sea shark-katran in the summer fishing period (May – October) is investigated. With the aim of increasing the content of omega-3 PUFA in the product method of winterization was applied. Crystallization of fat from the liver of sharks-katran dogfish was conducted in a special chiller with temperature from 0 to –5°C. During cooling, the fat is slowly stirred (speed of rotation of the mixer 20–25 rpm) for uniform cooling throughout. Full crystallization of SFA under these conditions was held for 5–6 h. The cooled fat was immediately sent to precipitating in the centrifuge. Quality of fat – transparency at a temperature of 15°C and unsaturation – iodine number of fat was selected as the optimization parameters of the process. It is established that: the temperature of the refrigerated fat, the duration of centrifugation, the number of drum rotations of the centrifuge are factors that significantly affect the iodine number and the organoleptic indicators of fat. Scheme two-level full factorial experiment was applied for modeling the values of iodine number and organoleptic indicator in changing these factors. Natural values of factors corresponding to the best value of indicator of the quality of the fat: temperature of cooling fat –4°C, duration of centrifugation 30 min, and the speed of rotation of the drum centrifuge 6000 rpm were determined in the experiment. The result of this treatment, the total unsaturation of the liquid fraction increased from 122,2–138,0 up to 131,0–145,0% of iodine number. The purified obtained fat was transparent, had a light yellow color, low acid value and peroxide values. In the obtained product the content of PUFA increased by 10–30%. The content of unsaponifiables in it, including biologically active substances alkylglycerols, was not decreased. The biological effectiveness of fat increased from 0,6 to 0,9.

scholarly journals The Dark Side of Microalgae Biotechnology: A Heterotrophic Biorefinery Platform Directed to ω-3 Rich Lipid Production

2019 ◽  
Vol 7 (12) ◽  
pp. 670 ◽  
Author(s):  
Teresa Lopes da Silva ◽  
Patrícia Moniz ◽  
Carla Silva ◽  
Alberto Reis
Keyword(s):  
Low Cost ◽  
Lipid Production ◽  
Arable Land ◽  
Integrated System ◽  
Dark Side ◽  
Omega 3 ◽  
Niche Markets ◽  
Microalgal Biomass ◽  
Oleaginous Microalgae ◽  
Heterotrophic Microalgae

Microbial oils have been considered a renewable feedstock for bioenergy not competing with food crops for arable land, freshwater and biodiverse natural landscapes. Microalgal oils may also have other purposes (niche markets) besides biofuels production such as pharmaceutical, nutraceutical, cosmetic and food industries. The polyunsaturated fatty acids (PUFAs) obtained from oleaginous microalgae show benefits over other PUFAs sources such as fish oils, being odorless, and non-dependent on fish stocks. Heterotrophic microalgae can use low-cost substrates such as organic wastes/residues containing carbon, simultaneously producing PUFAs together with other lipids that can be further converted into bioenergy, for combined heat and power (CHP), or liquid biofuels, to be integrated in the transportation system. This review analyses the different strategies that have been recently used to cultivate and further process heterotrophic microalgae for lipids, with emphasis on omega-3 rich compounds. It also highlights the importance of studying an integrated process approach based on the use of low-cost substrates associated to the microalgal biomass biorefinery, identifying the best sustainability methodology to be applied to the whole integrated system.

First Use of Handheld Raman Spectroscopy to Analyze Omega-3 Fatty Acids in Intact Fish Oil Capsules

2019 ◽  
Vol 74 (3) ◽  
pp. 365-371 ◽  
Author(s):  
Daniel Patrick Killeen ◽  
Asli Card ◽  
Keith Christopher Gordon ◽  
Nigel Brian Perry
Keyword(s):  
Fatty Acids ◽  
Fish Oil ◽  
Methyl Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Omega 3 Fatty Acids ◽  
Least Squares Regression ◽  
Omega 3 ◽  
Raman Spectrometer ◽  
Mean Square Errors ◽  
Ft Raman

Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are commercially important omega-3 fatty acids found in fish oils. Here we demonstrate that a handheld Raman spectrometer can be used to quantitate these compounds in intact fish oil capsules, avoiding oxidizing risk. Partial least squares regression models were prepared by relating Raman spectral variance to EPA and DHA concentrations determined using gas chromatography–mass spectrometry (GC-MS) analysis of fatty acid methyl esters in 15 commercial samples containing 145–473 mg g−1 EPA and 101–260 mg·g−1 DHA. Handheld Fourier transform (FT)-Raman models had root mean square errors of cross-validation of 38 mg g−1, 24 mg g−1, and 32 mg·g−1 for EPA, DHA, and EPA+DHA, respectively. Models generated from a benchtop FT-Raman spectrometer had corresponding errors of 32 mg·g−1, 22 mg·g−1, and 26 mg·g−1. By comparison, average standard deviations from triplicate GC-MS analyses were 11 mg·g−1 for EPA and 9 mg·g−1 for DHA.

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