scholarly journals Kinetic Studies on Radical Scavenging Activity of Kaempferol Decreased by Sn(II) Binding

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1975 ◽  
Author(s):  
Zhi-Yin Yang ◽  
Ling-Ling Qian ◽  
Yi Xu ◽  
Meng-Ting Song ◽  
Chao Liu ◽  
...  
Keyword(s):  
Fruits And Vegetables ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Kinetic Studies ◽  
Oxidation Potential ◽  
Scavenging Activity ◽  
Antioxidant Efficiency ◽  
Dpph Scavenging ◽  
Scavenging Efficiency ◽  
Package Materials

Sn(II) binds to kaempferol (HKaem, 3,4′,5,7-tetrahydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one) at the 3,4-site forming [Sn(II)(Kaem)2] complex in ethanol. DPPH• scavenging efficiency of HKaem is dramatically decreased by SnCl2 coordination due to formation of acid inhibiting deprotonation of HKaem as ligands and thus reduces the radical scavenging activity of the complex via a sequential proton-loss electron transfer (SPLET) mechanism. Moderate decreases in the radical scavenging of HKaem are observed by Sn(CH3COO)2 coordination and by contact between Sn and HKaem, in agreement with the increase in the oxidation potential of the complex compared to HKaem, leading to a decrease in antioxidant efficiency for fruits and vegetables with Sn as package materials.

scholarly journals ANTIOXIDANT ACTIVITY TEST OF ANDROGRAPHOLIDE IN BITTER HERBS USING DPPH SCAVENGING

2017 ◽  
Vol 1 (1) ◽  
pp. 9
Author(s):  
Ni Made Pitri Susanti ◽  
Ni Kadek Warditian ◽  
I Made Agus Gelgel Wirasuta
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Pharmacological Activities ◽  
Inhibition Effect ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Scavenging ◽  
Anti Inflammation

Abstract Bitter herbs (Sambiloto) have many pharmacological activities including antioxidant, antidiabetic, anticancer, antihyperlipidemic and anti-inflammation. Andrographolide is a diterpene compounds contained in bitter herbs. It is known that andrographolide compound responsible for the pharmacological activity of the bitter herbs. This study investigated DPPH free radical scavenging activity from andrographolide diterpene lactone. This study was initiated with the isolation of andrographolide compound from bitter herbs and then testing their DPPH free radical scavenging. The results suggested that andrographolide had IC50 value of 5.45 mg. This means andrographolide has 50% DPPH inhibition effect, i.e 5.45 mg.

scholarly journals Evaluation of the DPPH Radical Scavenging Activity, Total Phenolic Content and Total Flavanoid Content of Different Solvent Extracts of Catunaregam tomentosa (Blume ex DC) Tirveng Leaves

2021 ◽  
Vol 12 (2) ◽  
pp. 1-7
Author(s):  
Wan Amalina Wan Mamat ◽  
Syed Ahmad Tajudin Tuan Johari ◽  
Muhammad Yusran Abdul Aziz ◽  
Ahmad Syibli Othman ◽  
Abdul Manaf Ali
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
North East ◽  
The North ◽  
East Region ◽  
Dpph Scavenging

Catunaregam tomentosa (Blume ex DC) Tirveng is commonly known as Khet in Thailand and Bisa Ular or Badang in Malaysia. The tree is widely distributed in the north-east region of Thailand while in Malaysia the tree usually grows in the open waterfront area at Terengganu. This plant belongs to the Rubiaceae family, and the genus catunaregam has interesting pharmacological activities such as anti-inflammatory, antispasmodic, antidysenteric, antifertility and immunomodulatory. In this study, the leaves were extracted using dichloromethane, ethyl acetate and ethanol. Total phenolic was determined by Folin-Ciocalteau method while total flavonoid was determined by the aluminium chloride calorimetric method. Meanwhile, its antioxidant activity was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The ethanolic extract was found to have the highest percentages of phenolic and flavonoid content. Interestingly, ethanolic extract also demonstrated strong DPPH scavenging activity with IC50 at 20.07 ± 0.51µg/mL.

scholarly journals DEVELOPMENT AND EVALUATION OF HERBAL ANTIAGING CREAM FORMULATION CONTAINING ANNONA SQUAMOSA LEAF EXTRACT

2019 ◽  
pp. 210-214
Author(s):  
VANDANA MAHAWAR ◽  
KALPANA PATIDAR ◽  
NEELAM JOSHI
Keyword(s):  
Ascorbic Acid ◽  
Free Radical ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Annona Squamosa ◽  
Dpph Method ◽  
Dpph Scavenging

Objective: The aim of this research article is to develop and evaluate herbal antiaging cream using Annona squamosa leaf extract because of its antioxidant potential. Method: Free radical scavenging activity of Annona squamosa aqueous was determined by DPPH method. 0.5 ml of each solution of concentration of sample was added to 3 ml of 0.004% ethanolic DPPH free radical solution. After 30 minutes the absorbance of the preparations were taken at 517 nm by a UV spectrophotometer which was compared with the corresponding absorbance of standard ascorbic acid. The qualitative phytochemical analysis of Annona squamosa (custard apple) leaf extract shows the presence of flavonoids, tannins, alkaloids and phenols and absence of terpenoids and steroids Results: Formulation A3 shows good DPPH scavenging activity as compare to formulation A1 and A2 and ascorbic acid. Formulation A3 is stable for 3 months. Conclusion: Due to high antioxidant values of Annona squamosa it is concluded that it is possible to develop anti-aging cream using aqueous extract of Annona squamosa leaf.

scholarly journals Effect of grinding and extraction conditions on the determination of antioxidant activity and phenolic acids in barley

2021 ◽  
Author(s):  
Bing Zhou ◽  
Zhao Jin ◽  
Paul B. Schwarz ◽  
Yin Li
Keyword(s):  
Phenolic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Scavenging

AbstractIn the present study, the objective was to evaluate the effects of barley kernel size, grind level, and extract solvent on the antioxidant activities associated with total phenolic content and phenolic acid compositions. Three barley varieties (Kindred, Azure, and Tradition), were used and the results showed that with the exception of superoxide anion radical scavenging activity, thin kernel size fraction (2.0 mm) showed much higher antioxidant activities and total phenolic content than those in the 2.4 mm and 2.8 mm fractions when averaged across barley cultivar, grind level, and extract solvent. A similar trend was found for individual phenolic acid compositions among kernel sizes. Simple correlation analysis revealed that total phenolic content showed strong correlation with DPPH· scavenging activity, ABTS + scavenging activity, and reducing power. Generally, fine grind and 80% acetone extract showed averaged highest values in antioxidant activities, total phenolic content, and individual phenolic acid compositions. Stepwise linear regression showed that extract solvent was the most important factor for DPPH· scavenging activity, ABTS + scavenging activity, reducing power, and iron chelating activity. The sum of syringic acid and caffeic acid exhibited a dominant role in explaining the major variation in antioxidant activities except for superoxide anion radical scavenging activity.

scholarly journals Complexes of 1,3-Diisobutyl Thiourea with Copper(I), Zinc(II) and Mercury(II): Their Antioxidant and Antibacterial Evaluation

Crystals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 989
Author(s):  
Adnan Shahzad ◽  
Ezzat Khan ◽  
Muhammad Said ◽  
Gul Shazada Khan ◽  
Mian Gul Syed ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Data Set ◽  
Standard Drug ◽  
E Coli ◽  
Scavenging Efficiency

The reaction of 1,3-Diisobutyl thiourea (Tu) with metal salts, {[CuX (X = Cl, I)], [ZnCl2] and [HgI2] in an appropriate stoichiometric ratio afforded the corresponding metal complexes [Tu2CuCl] (1), [Tu3CuI] (2), [Tu2ZnCl2] (3) and [Tu2HgI2] (4) in good yields. The FT-IR data show typically broad signals (3278–3288 cm−1) attributed to the involvement of NH bonds in extensive hydrogen bonding. The structures of complexes were proposed based on a spectroscopic data set. Compounds 1 and 2 were additionally characterized by single-crystal X-ray analysis. Complexes 1–4 were tested for their free radical scavenging efficiency using 2,2-diphenyl-1-picrylhydrazyl free radical (hereafter abbreviated as DPPH). The free radical scavenging activity was a function of decrease in the resultant absorption of DPPH solution after the mixing of an appropriate concentration of the respective complex. The activity of complexes was determined to be dose dependent and increased concentration of the complex resulted in improved antioxidant activity. Compound 1 was found to be the most efficient, with 79.9% free radical scavenging activity. Complexes were also tested for their efficiency against selected strains of bacteria (E. coli, S. flexneri, S. typhi, and P. aeruginosa) and the activities were compared to commercially available standard drug cephradine. Compound 1 was more active against P.aeruginosa (ZI 13.25), while compound 4 was found to be more active against E. coli (ZI 11.0), S. flexneri (ZI 11.2), and S. typhi (ZI 10.5).

scholarly journals Effect Of Five Proteases Including Alcalase, Flavourzyme, Papain, Proteinase K And Trypsin On Antioxidative Activities Of Casein Hydrolysate From Goat Milk

2015 ◽  
Vol 19 (2) ◽  
pp. 65-74 ◽  
Author(s):  
Guowei Shu ◽  
Qian Zhang ◽  
He Chen ◽  
Hongchang Wan ◽  
Hong Li
Keyword(s):  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Casein Hydrolysate ◽  
Goat Milk ◽  
The Body ◽  
Proteinase K ◽  
Scavenging Activity ◽  
Dpph Scavenging ◽  
Casein Hydrolysates

Abstract Oxidation was related to the pathogenesis of human diseases. Adequate intake of antioxidant activity of food can reduce the levels of free radicals, prevent lipid peroxidation, and help the body against diseases. In the paper, casein from goat milk was hydrolyzed by five commercial proteases, namely, Alcalase, flavourzyme, papain, proteinase K and trypsin. The antioxidant activities of casein hydrolysates were assessed by evaluating hydrolysis degree, DPPH radical-scavenging activity, metal-chelating activity and superoxide radical scavenging activity. The results showed as follows: the DH of proteinase K, Alcalase, and trypsin were higher significantly than those of papain and flavourzyme. The Fe2+-chelation activity and superoxide radical scavenging activity of casein hydrolysates from goat milk by Alcalase was higher than the others, the DPPH scavenging activities of casein hydrolysates by Alcalase and papain was higher than the others and the DPPH scavenging activities by Alcalase and papain had no significant diffierence (p<0.05), so the optimal proteinase for hydrolysis casein from goat milk to produce antioxidant peptide was Alcalase.

Preparation, Purification and Identification of Antioxidant Peptides with Bienzyme Hydrolysis from Rice Bran Protein

2012 ◽  
Vol 610-613 ◽  
pp. 72-80 ◽  
Author(s):  
De Jun Mei ◽  
Guo Ping Yu ◽  
An Min Sun
Keyword(s):  
Rice Bran ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Antioxidant Peptides ◽  
Rice Bran Protein ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Dpph Radical Scavenging

The rice bran protein (RBP) was then hydrolyzed with various proteases (papain, flavorzyme, neutrase, protamex, and trypsin) to prepare antioxidant peptides. The rice bran protein hydrolysates (RBPH) were assessed using method of DPPH radical scavenging ability. Hydrolysate prepared with papain and flavorzyme (activity ratio 1:1) was found to have the highest antioxidant activity (IC50=6.778±0.21 mg/ml). This hydrolysate was purified using ultrafiltration, RBPH-III (Mw＜3KDa) had the highest DPPH and hydroxyl radical scavenging activity (IC50 value of 6.56±0.28, 5.43±0.22, respectively) and highest reducing power activity (1.02±0.18 at 4 mg/mL). Later, RBPH-III was fractionated by SP-SephadexC-25 cation-exchange column into six fractions (A–F), fraction F with the highest DPPH scavenging activity, was then separated by size exclusion chromatography on a SephadexG-25 into three major fractions (F1–F3). Fraction F2 exhibited the highest DPPH scavenging activity was choose to fractionate by reversed-phase high performance liquid chromatography (RP-HPLC), seven antioxidant peptides were isolated, The F2-5 peptide displayed the highest DPPH radical-scavenging activity (58.2±1.63%; at 250 μg/ml) among these peptides, the amino acids composition of F2-5 was determined, which might play an important role on its antioxidant activity. In addition, purified peptide did show remarkable inhibition rate on SGC-7901 cells proliferation, and it also revealed the dose-dependent relationship. The results of this study suggest that rice bran protein hydrolysates are good source of natural antioxidants.

scholarly journals Polyphenol Contents and Radical-Scavenging Activity of Extracts from Fruits and Vegetables in Cultivated in Okinawa, Japan

2005 ◽  
Vol 52 (10) ◽  
pp. 462-471 ◽  
Author(s):  
Ikuo Suda ◽  
Tomoyuki Oki ◽  
Yoichi Nishiba ◽  
Mami Masuda ◽  
Mio Kobayashi ◽  
...  
Keyword(s):  
Fruits And Vegetables ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity

scholarly journals Peroxyl radical scavenging activity of soluble high molecular weight fraction from coffee brews

2004 ◽  
Vol 22 (SI - Chem. Reactions in Foods V) ◽  
pp. S212-S214
Author(s):  
C. Delgado-Andrade ◽  
F. J Morales
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Peroxyl Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
High Molecular Weight Fraction ◽  
Scavenging Activity ◽  
Antioxidant Efficiency ◽  
Coffee Brew ◽  
Coffee Beans

Roasting process of coffee beans affects the final composition of coffee brew. While the presence of some of its antioxidant compounds (phenolic acids) decrease throughout the treatment, melanoidins are formed during the thermal process. Recent research into nutritional, physiological and functional properties of melanoidins has suggested that they have antioxidant activity. Instant coffees produced from roasted coffee beans were obtained from a company coffee in three different roasting degrees: light (CTn 110), medium (CTn 85) and dark (CTn 60). Melanoidins of high molecular weight (&gt; 10 000 Da) were obtained from each coffee, as well as pure melanoidins by incubation in 2M ClNa and ultrafiltration. CGA contained in extractes of different melanoidins (1 mg/ml) was measured by HPLC. Peroxyl radical scavenging activity of melanoidins and pure melanoidins were tested using a procedure to inhibite the peroxidation of linoleic acid. Antioxidant efficiency (AE<sub>50</sub>, ml/min/mg) was calculated as the slope of the time of inhibition at 50% (min) against concentration (mg/ml). No significant differences were found between melanoidins and pure melanoidins in the different roasting degrees. Peroxyl radical scavenging activity of melanoidins from coffee brew is mainly due to the chelated compounds by ionic binds.

scholarly journals A Study on Organic Tomatoes: Effect of a Biostimulator on Phytochemical and Antioxidant Activities

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Varinder Sidhu ◽  
Dilip Nandwani ◽  
Li Wang ◽  
Ying Wu
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Treatment Groups ◽  
Significant Difference ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The objective of the present study is to investigate nutritional and antioxidant activity of four types of organic tomato cultivars. The differences in tomato quality are also tested between groups with or without treatment using an organic biostimulator, Stimplex. Total phenolic compounds (TPC), lycopene,β-carotene, DPPH free radical scavenging activity, reducing power, and color parameters were investigated in the current study. The results showed that there was no significant difference in TPC among cultivars regardless of Stimplex treatment. Higher lycopene andβ-carotene were obtained in Stimplex treated tomatoes. Lycopene andβ-carotene contents were significantly different among cultivars (P<0.05). DPPH scavenging activity in controlled group was significantly higher than that in the Stimplex treated tomatoes (P<0.05). No significant difference in reducing power was detected among cultivars treatment groups. The study showed that the darker the tomato color, the higher the lycopene andβ-carotene contents and the stronger the reducing power.

Sign in / Sign up

Export Citation Format

Share Document