scholarly journals Chemical Profile, In Vitro Biological Activity and Comparison of Essential Oils from Fresh and Dried Flowers of Lavandula angustifolia L.

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5317
Author(s):  
Claudio Caprari ◽  
Francesca Fantasma ◽  
Fabio Divino ◽  
Antonio Bucci ◽  
Maria Iorizzi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Fruits And Vegetables ◽  
Central Italy ◽  
Radical Scavenging ◽  
Sclerotium Rolfsii ◽  
Bactericidal Effect ◽  
Phytopathogenic Fungus ◽  
Lavandula Angustifolia

The chemical composition of essential oils (EOs) from dried and fresh flowers of Lavandula angustifolia L. (lavender), named LA 2019 and LA 2020, respectively, grown in central Italy was analyzed and compared by GC and GC-MS. For both samples, 61 compounds were identified, corresponding to 97.9% and 98.1% of the total essential oils. Explorative data analysis, performed to compare the statistical composition of the samples, resulted in a high level of global similarity (around 93%). The compositions of both samples were characterized by 10 major compounds, with a predominance of Linalool (35.3–36.0%), Borneol (15.6–19.4%) and 1,8-Cineole (11.0–9.0%). The in vitro antibacterial activity assay by disk diffusion tests against Bacillus subtilis PY79 and Escherichia coli DH5α showed inhibition of growth in both indicator strains. In addition, plate counts revealed a bactericidal effect on E. coli, which was particularly noticeable when using oil from the fresh lavender flowers at the highest concentrations. An in vitro antifungal assay showed that the EOs inhibited the growth of Sclerotium rolfsii, a phytopathogenic fungus that causes post-harvest diseases in many fruits and vegetables. The antioxidant activity was also assessed using the ABTS free radical scavenging assay, which showed a different antioxidant activity in both EOs. In addition, the potential application of EOs as a green method to control biodeterioration phenomena on an artistic wood painting (XIX century) was evaluated.

scholarly journals Antioxidant and antimicrobial activities of Lavandula angustifolia Mill. field-grown and propagated in vitro

2017 ◽  
Vol 29 (2) ◽  
pp. 161-180 ◽  
Author(s):  
Dominika Andrys ◽  
Danuta Kulpa ◽  
Monika Grzeszczuk ◽  
Magdalena Bihun ◽  
Agnieszka Dobrowolska
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oils ◽  
Root System ◽  
Antimicrobial Activities ◽  
Positive Influence ◽  
Ms Medium ◽  
Lavandula Angustifolia ◽  
Number Of Shoots

AbstractIn the study, micropropagation of three varieties of Lavandula angustifolia was developed, and the appearance of trichomes, antioxidant activity of extracts and antimicrobial activity of essential oils isolated from plants growing in field conditions and in vitro cultures were compared. The study evaluated the number of shoots, and the height and weight of the plants grown on media with additions of BAP, KIN and 2iP. The greatest height was attained by the lavenders growing on MS medium with the addition of 1 mg dm-32iP - ‘Ellagance Purple’. The greatest number of shoots was developed by the ‘Ellagance Purple’ and ‘Munstead’ plants growing on the medium with 2 mg dm-3BAP. The highest weight was attained by the plants growing on the medium with the highest concentration of BAP - 3 and 5 mg dm-3. Moreover, the present study determined the influence of media with the addition of different concentrations of IBA and media with a variable mineral composition (½, ¼, and complete composition of MS medium) and with the addition of IBA or NAA for rooting. The majority of the media used had a positive influence on the development of the root system. The longest root system was observed in ‘Ellagance Purple’ growing on the medium composed of ¼ MS with 0.2 mg dm-3NAA. All the examined oils exhibited activity towards S. aureus, S. epidermidis, P. aeruginosa, E. coli and C. albicans. The majority of the essential oils isolated from the plants propagated in vitro exhibited stronger antimicrobial activity than the field-grown plants. The plants propagated under in vitro conditions demonstrated considerably higher antioxidant activity as compared with the field-grown plants, which was determined using the DPPH, FRAP and ABTS assay.

scholarly journals Prunus spinosa Fresh Fruit Juice: Antioxidant Activity in Cell-free and Cellular Systems

2009 ◽  
Vol 4 (12) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Daniele Fraternale ◽  
Laura Giamperi ◽  
Anahi Bucchini ◽  
Piero Sestili ◽  
Marco Paolillo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Fruit Juice ◽  
Central Italy ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Fresh Fruit ◽  
Cellular Systems ◽  
Anthocyanin Content ◽  
Prunus Spinosa

The antioxidant activity was assessed of fresh juice from Prunus spinosa L. fruit (Rosaceae) growing wild in Urbino (central Italy) by using different cell-free in vitro analytical methods: 5-lipoxygenase test, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, and oxygen radical absorbance capacity (ORAC). Trolox was used as the reference antioxidant compound. In the 5-lipoxygenase and DPPH tests the fresh fruit juice of P. spinosa showed good antioxidant activity when compared with Trolox, while the ORAC value was 36.0 μmol eq. Trolox /g of fruit. These values are in accord with data reported in the literature for small fruits such as Vaccinium, Rubus and Ribes. The antioxidant capacity in cell-free systems of P. spinosa juice has been compared with its cytoprotective – bona fide antioxidant activity in cultured human promonocytes (U937 cells) exposed to hydrogen peroxide. The antioxidant activity of red berries has been correlated with their anthocyanin content. The results of this study indicate that the three most representative anthocyanins in P.spinosa fruit juice (cyanidin-3-rutinoside, peonidin-3-rutinoside and cyanidin-3-glucoside) are likely to play an important role in its antioxidant properties.

scholarly journals Chemical Composition and Antioxidant Properties of Essential Oils from Peppermint, Native Spearmint and Scotch Spearmint

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2825 ◽  
Author(s):  
Zhaohai Wu ◽  
Bie Tan ◽  
Yanhong Liu ◽  
James Dunn ◽  
Patricia Martorell Guerola ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
C Elegans ◽  
Reducing Power Assay

Natural antioxidants have drawn growing interest for use in animal feed and the food industry. In the current study, essential oils (EOs) obtained from hydrodistillation of three mentha species, including Mentha piperita (peppermint), Mentha spicata (native spearmint) and Mentha gracilis (Scotch spearmint), harvested in the Midwest region in the United States, were analyzed for their chemical composition using gas chromatography-mass spectrometry, and their antioxidant properties were assessed through chemical assays, in vitro cell culture modeling and in Caenorhabditis elegans (C. elegans). The activity of ferric iron reduction and free-radical scavenging capacity were assessed through chemical-based assays, including the reducing power assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, and Trolox equivalent antioxidant capacity assay (TEAC). Subsequently, the capacity of EOs to mitigate lipid peroxidation was analyzed at various doses using fresh liver homogenates from pigs. A porcine jejunum epithelial cell line (IPEC-J2) was employed as in vitro model to study the cellular antioxidant activity of the mint EOs. Finally, the effectiveness of mint EOs to alleviate acute systemic oxidative damage were evaluated in vivo using C. elegans. Data were analyzed by the MIXED procedure of SAS. Contrast statement was performed to assess linear or quadratic effects of mint EOs given at various doses. All three EOs are mostly composed of monoterpenes and their derivatives (76–90%), but differed in the major compounds, which are menthol and menthone (50%) in peppermint EO and carvone (70%) in spearmint EOs. Three mint EOs demonstrated prominent radical scavenging and Fe3+ reducing activity in chemical-based assays. In comparison with native and Scotch spearmint EOs, peppermint EO had the lowest (p < 0.05) half maximal effective concentration (EC50) in DPPH and TEAC assays and higher efficacy in the reducing power assay. All three EOs exhibited equivalent activity in mitigation of chemical-induced lipid peroxidation in liver tissues in a dose-dependent manner (linear, p < 0.001). The maximal cellular antioxidant activity (CAA) was observed at 5 µg/mL for peppermint, and 100 µg/mL for native and Scotch spearmint EOs. The addition of 25 µg/mL of both spearmint EOs increased (p < 0.05) cellular concentrations of glutathione in H2O2-treated IPEC-J2 cells, suggesting enhanced endogenous antioxidant defense. Supplementation of 100 µg/mL of peppermint or Scotch spearmint EO significantly increased (p < 0.05) the survival rate of C. elegans in response to H2O2-induced oxidative stress. The protective effect is comparable to that of supplementation of 10 µg/mL of ascorbic acid. However native spearmint EO failed to reduce the death rate within the same supplementation dose (10–200 μg/mL).

scholarly journals ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC CONTENT OF ESSENTIAL OILS, AND EXTRACT DETERMINED FROM NATURAL LEAVES, IN-VITRO LEAVES, AND CALLUS SOURCES OF MELALEUCA ALTERNIFOLIA – A COMPARATIVE STUDY

2021 ◽  
pp. 141-143
Author(s):  
JEYAKANI M ◽  
RAJALAKSHMI M
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Melaleuca Alternifolia ◽  
Biological Potential

Objectives: Essential oil and their compounds are getting increasing interest due to their multipurpose functional as alternatives to artificial preservatives. The aim of this study was focused to comparative analyses on hydrodistillation, total phenolic content (TPC), and antioxidant activity of essential oil derived from Melaleuca alternifolia (M. alternifolia) leaves procured from natural leaves, in-vitro leaves, and callus sources. Methods: The essential oil was extracted using hydrodistillation. The Folin-Ciocalteu method was used to determine the TPC equivalent and antioxidant activity of essential oils. Results: TPC of essential oil from in-vitro sources of leaves contained 14.79 mg gallic acid equivalent/g dry plant material and its IC50 value was found to be 70% in 1-diphenyl 2-picrylhyorazyl assay. Thus, this source of essential oil showed good free radical scavenging activity. Conclusion: The estimated biological potential was obtained in the essential oil from in-vitro sources of leaves M. alternifolia.

scholarly journals Phytochemical and Antioxidant Activity of the Essential Oils of Satureja briquetii L. from Morocco

2018 ◽  
Vol 10 (02) ◽  
Author(s):  
Zineb Benziane Ouaritini ◽  
Smahane Boukhira ◽  
El houssaine Derwich
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Aerial Part ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Ic50 Value ◽  
Chromatographic Profile ◽  
Fresh Aerial Part ◽  
Dpph Radical Scavenging

Satureja briquetii L. (Labiatae) species are a well-known aromatic plant which is used to produce essential oils and aromatic water in the mountain regions of Sefrou part of Morocco. In our study, it was aimed to determine phytochemical and antioxidant activities of Satureja briquetii L. essential oils in vitro. Antioxidant activities of the oils at differents concentrations were evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging. The extraction of essential oils from aerial part of this plant is carried out by hydrodistillation, and yields are 1.75% for dry aerial part and 1.05% for fresh aerial part. The analysis of the essential oils of dry aerial part from Satureja briquetii L. by GC/MS identified 57 principles compounds including Spathulenol (9.81%), Verbenone (4.35%), Camphene (3.56%), Himachalene (3,2%) and Cedrene (2,12%). The chromatographic profile of essential oils from fresh aerial part of Satureja briquetii L. has 49 constituents representing 96.25% of the essential oil, where the Menth-8-ene (14.99%), Cymene (4.97%) and Carene (4.97%) are major compounds. The Menth-1,4(8)-diene, (1,06%), Cubenol and Longifolene (3,44%) are in minority. Furthermore, the antioxidant activity of the essential art of Satureja briquetii L. was evaluated by the method of DPPH, and showed a significant efficiency in radical DPPH reducing with an IC50 value of of 31.027 ± 0.586 µg/ml from essential oils of dry aerial and 35.034 ± 0.0432 µg/ml in essential oils from fresh aerial part.

Curcumin-based antioxidant and glycohydrolase inhibitor compounds: Synthesis and in vitro appraisal of the dual activity against diabetes

2020 ◽  
Vol 16 ◽  
Author(s):  
Sajjad Esmaeili ◽  
Nazanin Ghobadi ◽  
Donya Nazari ◽  
Alireza Pourhossein ◽  
Hassan Rasouli ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Enzyme Inhibitor ◽  
Radical Scavenging Activity ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Inhibitory Effect ◽  
Curcumin Derivatives ◽  
Reducing Power Assay

Background: Curcumin, as the substantial constituent of the turmeric plant (Curcuma longa), plays a significant role in the prevention of various diseases, including diabetes. It possesses ideal structure features as enzyme inhibitor, including a flexible backbone, hydrophobic nature, and several available hydrogen bond (H-bond) donors and acceptors. Objective: The present study aimed at synthesizing several novel curcumin derivatives and further evaluation of these compounds for possible antioxidant and anti-diabetic properties along with inhibitory effect against two carbohydrate-hydrolyzing enzymes, α-amylase and α-glucosidase, as these enzymes are therapeutic targets for attenuation of postprandial hyperglycemia. Methods: Therefore, curcumin-based pyrido[2,3-d]pyrimidine derivatives were synthesized and identified using an instrumental technique like NMR spectroscopy and then screened for antioxidant and enzyme inhibitory potential. Total antioxidant activity, reducing power assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH• ) radical scavenging activity were done to appraisal the antioxidant potential of these compounds in vitro. Results: Compounds L6-L9 showed higher antioxidant activity while L4, L9, L12 and especially L8 exhibited the best selectivity index (lowest α-amylase/α-glucosidase inhibition ratio). Conclusion: These antioxidant inhibitors may be potential anti-diabetic drugs, not only to reduce glycemic index but also to limit the activity of the major reactive oxygen species (ROS) producing pathways.

Synthesis and antioxidant activity of new selenium-containing quinolines

2020 ◽  
Vol 16 ◽  
Author(s):  
Benedetta Bocchini ◽  
Bruna Goldani ◽  
Fernanda S.S. Sousa ◽  
Paloma T. Birmann ◽  
Cesar A. Brüning ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Radical Scavenging ◽  
Building Blocks ◽  
Antioxidant Potential ◽  
In Vitro Assays ◽  
Pharmacological Activities ◽  
Antioxidant Power ◽  
Antioxidant Agents

Background: Quinoline derivatives have been attracted much attention in drug discovery and synthetic derivatives of these scaffolds present a range of pharmacological activities. Therefore, organoselenium compounds are valuable scaffolds in organic synthesis because their pharmacological activities and their use as versatile building blocks for regio-, chemio-and stereoselective reactions. Thus, the synthesis of selenium-containing quinolines has great significance, and their applicability range from simple antioxidant agents, to selective DNA-binding and photocleaving agents. Objective: In the present study we describe the synthesis and antioxidant activity in vitro of new 7-chloroN(arylselanyl)quinolin-4-amines 5 by the reaction of 4,7-dichloroquinoline 4 with (arylselanyl)-amines 3. Methods: For the synthesis of 7-chloro-N(arylselanyl)quinolin-4-amines 5, we performed the reaction of (arylselanyl)- amines 3 with 4,7-dichloroquinoline 4 in the presence of Et3N at 120 °C in a sealed tube. The antioxidant activities of the compounds 5 were evaluated by the following in vitro assays: 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric ion reducing antioxidant power (FRAP), nitric oxide (NO) scavenging and superoxide dismutase-like activity (SOD-Like). Results: 7-Chloro-N(arylselanyl)quinolin-4-amines 5a-d has been synthesized in yields ranging from 68% to 82% by the reaction of 4,7-dichloroquinoline 4 with arylselanyl-amines 3a-d using Et3N as base, at 120 °C, in a sealed tube for 24 hours and tolerates different substituents, such as -OMe and -Cl, in the arylselanyl moiety. The obtained compounds 5a-d presented significant results with respect to the antioxidant potential, which had effect in the tests of inhibition of radical’s DPPH, ABTS+ and NO, as well as in the test that evaluates the capacity (FRAP) and in the superoxide dismutase-like activity assay (SOD-Like). It is worth mentioning that 7-chloro-N(arylselanyl)quinolin-4-amine 5b presented excellent results, demonstrating a better antioxidant capacity when compared to the others. Conclusion: According to the obtained results 7-chloro-N(arylselanyl)quinolin-4-amines 5 were synthesized in good yields by the reaction of 4,7-dichloroquinoline with arylselanyl-amines and tolerates different substituents in the arylselanyl moiety. The tested compounds presented significant antioxidant potential in the tests of inhibition of DPPH, ABTS+ and NO radicals, as well as in the FRAP and superoxide dismutase-like activity assays (SOD-Like).

scholarly journals Microwave-Assisted Synthesis, Characterization, Antimicrobial and Antioxidant Activity of Some New Isatin Derivatives

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
G. Kiran ◽  
T. Maneshwar ◽  
Y. Rajeshwar ◽  
M. Sarangapani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Microwave Assisted Synthesis ◽  
Stable Free Radical ◽  
Ft Ir ◽  
Antimicrobial And Antioxidant Activity ◽  
New Compounds ◽  
Isatin Derivatives

A series of β-Isatin aldehyde-N,N′-thiocarbohydrazone derivatives were synthesized and assayed for theirin vitroantimicrobial and antioxidant activity. The new compounds were characterized based on spectral (FT-IR, NMR, MS) analyses. All the test compounds possessed a broad spectrum of activity having MIC values rangeing from 12.5 to 400 μg/ml against the tested microorganisms. Among the compounds3e,3jand3nshow highest significant antimicrobial activity. The free radical scavenging effects of the test compounds against stable free radical DPPH (α,α-diphenyl-β-picryl hydrazyl) and H2O2were measured spectrophotometrically. Compounds3j,3n,3l, and3e, respectively, had the most effective antioxidant activity against DPPH and H2O2scavenging activity.

scholarly journals Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1081
Author(s):  
Matilda Rădulescu ◽  
Călin Jianu ◽  
Alexandra Teodora Lukinich-Gruia ◽  
Marius Mioc ◽  
Alexandra Mioc ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
In Silico ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Melissa Officinalis ◽  
Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

scholarly journals Phytochemical Fingerprinting and In Vitro Antimicrobial and Antioxidant Activity of the Aerial Parts of Thymus marschallianus Willd. and Thymus seravschanicus Klokov Growing Widely in Southern Kazakhstan

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3193
Author(s):  
Bagda Sagynaikyzy Zhumakanova ◽  
Izabela Korona-Głowniak ◽  
Krystyna Skalicka-Woźniak ◽  
Agnieszka Ludwiczuk ◽  
Tomasz Baj ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protocatechuic Acid ◽  
Antioxidant Activities ◽  
Methyl Esters ◽  
Bactericidal Effect ◽  
Ethyl Esters ◽  
Volatile Fraction ◽  
Aerial Parts ◽  
Antimicrobial And Antioxidant Activity

The chemical composition of the hydroethanolic extracts (60% v/v) from the aerial parts of Thymus marschallianus Willd (TM) and Thymus seravschanicus Klokov (TS) from Southern Kazakhstan flora was analyzed together with their hexane fractions. Determination of antibacterial, antifungal and antioxidant activities of both extracts was also performed. RP-HPLC/PDA and HPLC/ESI-QTOF-MS showed that there were some differences between the composition of both extracts. The most characteristic components of TM were rosmarinic acid, protocatechuic acid, luteolin 7-O-glucoside, and apigenin 7-O-glucuronide, while protocatechuic acid, luteolin 7-O-glucoside, luteolin 7-O-glucuronide, and eriodictyol predominated in TS. The content of polyplenols was higher in TS than in TM. The GC-MS analysis of the volatile fraction of both examined extracts revealed the presence of thymol and carvacrol. Additionally, sesquiterpenoids, fatty acids, and their ethyl esters were found in TM, and fatty acid methyl esters in TS. The antioxidant activity of both extracts was similar. The antibacterial activity of TS extract was somewhat higher than TM, while antifungal activity was the same. TS extract was the most active against Helicobacter pylori ATCC 43504 with MIC (minimal inhibitory concentration) = 0.625 mg/mL, exerting a bactericidal effect. The obtained data provide novel information about the phytochemistry of both thyme species and suggest new potential application of TS as a source of bioactive compounds, especially with anti-H. pylori activity.

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