scholarly journals Biological Activity of Essential Oils of Four Juniper Species and Their Potential as Biopesticides

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6358
Author(s):  
Ivanka Semerdjieva ◽  
Valtcho D. Zheljazkov ◽  
Tzenka Radoukova ◽  
Ivayla Dincheva ◽  
Neshka Piperkova ◽  
...  

The objective of this study was to assess the biological activity of essential oils (EOs) of four Juniperus species obtained via two different distillation methods and their potential as biopesticides. The studied factors were juniper species (Juniperus communis L., J. oxyсedrus L., J. pygmaea C. Koch., and J. sibirica Burgsd), plant sex (male (M) and female (F)), and distillation method (hydrodistillation via a standard Clevenger apparatus (ClevA) and semi-commercial (SCom) steam distillation). The hypothesis was that the EO will have differential antioxidant, antimicrobial, and insecticidal activities as a function of plant species, plant sex, and distillation method. The two distillation methods resulted in similar EO composition within a given species. However, there were differences in the EO content (yield) due to the sex of the plant, and also differences in the proportions of some EO components. The concentration of α-pinene, β-caryophyllene, δ-cadinene and δ-cadinol was dissimilar between the EO of M and F plants within all four species. Additionally, M and F plants of J. pygmaea, and J. sibirica had significantly different concentrations of sabinene within the respective species. The EOs obtained via ClevA extraction showed higher antioxidant capacity within a species compared with those from SCom extraction. All of the tested EOs had significant repellent and insecticidal activity against the two aphid species Rhopalosiphum padi (bird cherry-oat aphid) and Sitobion avenae (English grain aphid) at concentrations of the EO in the solution of 1%, 2.5%, and 5%. The tested EOs demonstrated moderate activity against selected pathogens Fusarium spp., Botrytis cinerea, Colletotrichum spp., Rhizoctonia solani and Cylindrocarpon pauciseptatum. The results demonstrate that the standard ClevA would provide comparable EO content and composition in comparison with SCom steam distillation; however, even slight differences in the EO composition may translate into differential bioactivity.

BioResources ◽  
2007 ◽  
Vol 2 (2) ◽  
pp. 265-269 ◽  
Author(s):  
M. Hakki Alma ◽  
Murat Ertaş ◽  
Siegfrie Nitz ◽  
Hubert Kollmannsberger

In this study, clove bud oil, which was cultivated in the Mediterranean region of Turkey, was provided from a private essential oil company in Turkey. Essential oil from clove (Syzygium aromaticum L.) was obtained from steam-distillation method, and its chemical composition was analyzed by GC and GC-MS. The results showed that the essential oils mainly contained about 87.00% eugenol, 8.01% eugenyl acetate and 3.56% β-Caryophyllene. The chemical composition of the Turkish clove bud oil was comparable to those of trees naturally grown in their native regions.


Author(s):  
N. A. Kovalenko ◽  
G. N. Supichenko ◽  
V. N. Leontiev ◽  
A. G. Shutova

The steam distillation method was used to obtain samples of essential oil of some species of genus Agastache L. introduced in Belarus. More than 20 components have been identified and determined in the essential oils of Agastache pallidiflora ssp.neomexicana L., Agastache aurantiaca L., Agastache mexicana L., Agastache foeniculum L. by gas liquid chromatography.The quantitative composition of the samples of essential oils depends on the type of plant material. The main its components are limonene, 1.8-cineol, mentone, isomentone, methyl chavicol, methyeugenol. The main component of essential oils Agastache pallidiflora ssp. neomexicana L. и Agastache aurantiaca L is mentone (40–65 %). Essential oil Agastache foeniculum L. is enriched with pulegone and isomentone. Methyl chavicol and methyleugenol dominate in Agastache mexicana essential oil. Distribution of the enantiomers of limonene, menthone and pulegone are shown. Regardless of the plant species, the samples are optically pure in the (+)-pulegone. The nature of the distribution of the enantiomers of limonene and menthone is an individual characteristic of the studied Agastache L. essential oils.


2018 ◽  
pp. 77
Author(s):  
I Made Suardhika

This study aims to determine the effect of drying time on the yield of essential oils from orange peel (Citrus sinensis) and identify the presence of linalool compounds from essential oils. 300 grams of orange skin samples were dried at 40ºC using an oven with a drying treatment of 0 hours (fresh), 24 hours, and 48 hours. The extraction process is carried out by steam distillation method with attainment of 500 ml of distillate. Essential oils are separated from water using liquidliquid extraction (LLE), so that the essential oils are maximally obtained. The highest yield of essential oil obtained in the sample with a drying treatment for 48 hours is 0.5% (MA3), 24-hour drying obtained a yield of 0.4% (MA2) and the sample without drying provides the smallest yield of 0.2% (MA1). Identification of linalool compounds using TLC-Spectrophotodensitometry was carried out to determine the amount of linalool content seen from the AUC (Area Under Curve). AUC MA3 sample is 39475, MA2 is 35889, and MA1 is 8980. Based on the data obtained can be concluded that the drying duration of the sample affects the yield of essential oils and linalool compounds that obtained.


2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Anna Kędzia ◽  
Elżbieta Hołderna-Kędzia

Introduction. Silver fir (Abies alba Mill.) grows in mountainous areas all over Europe. The tree reaches a height of 40 to 60 m. Essential oils obtained from the needles, cones and fir wood by steam distillation differing in composition. The oil obtained from the needles mainly contains L-α-pinene, L-limonene, santene, L-bornyl acetate and laurel aldehyde. In the oil obtained from cones L-limonene, L-α-pinene, borneol and L-bornyl acetate dominated. Fir oil has antioxidant, disinfecting and antiseptic properties. Aim. The aim of the research was to determine the activity of fir oil against yeast-like fungi. Material and methods. 61 strains of yeast-like fungi isolated from the oral cavity and 9 reference strains were used for the study. Susceptibility of yeast-like fungi to fir oil was determined by serial dilution in Sabouraud agar. The inoculum containing 105 CFU/drop was transferred with a Steers replicator to agar plates. The concentrations of the tested oil (Semifarm, Gdańsk) were 20.0, 15.0, 10.0, 7.5, 5.0 and 2.5 mg/ml. The oil-free agar was used to control the growth of the strains. Incubation was carried out in aerobic conditions at 37°C for 24-48 hours. The lowest concentration of oil that completely inhibited the growth of yeast-like fungi was assumed as the MIC. Results. The results of the research carried out indicate that fir oil showed moderate activity against fungi tested. Concentrations in the range ≤ 5.0-7.5 mg/ml inhibited the growth of 10 (16.3%) strains. However, 7.5-15.0 mg/ml of the oil was active against 48 (78.7%) strains. Only 3 (5%) strains were sensitive to 20.0 mg/ml oil or more. The C. utilis strains turned out to be the most sensitive (MIC = 2.5 mg/ml). Higher concentrations (≤ 2.5-10.0 mg/ml) inhibited the growth of C. glabrata and C. parapsilosis. C. krusei and C. kefyr strains were sensitive to 5.0-15.0 mg/ml of oil, C. guilliermondii to 7.5-10.0 mg/ml, C. lusitaniae to 7.5-≥ 20.0 mg/ml and C. humicola to 15.0 mg/ml. The oil was less active against C. tropicalis (MIC ≤ 2.5-≥ 20.0 mg/ml). Conclusions. Fir oil showed the greatest activity against strains of yeast-like fungi of the C. utilis species. C. glabrata strains were characterized by moderate susceptibility. The oil showed moderate activity against C. albicans, which is often involved in infections.


2019 ◽  
Vol 8 (1) ◽  
pp. 8-13
Author(s):  
Sophia G Sipahelut

The demand for essential oils has increased recently due to the growing of parfume, cosmetic, pharacentical, food and beverage, natural flavourings, medicines, aroma therapy, as well as non-food industries. Nutmeg fruit flesh is one of the potential sources of essential oils which can be obtained by both water distilation and water-steam distillation. Gas Chromatography-Mass Spectometry (GC-MS) is a dynamic analytical method to separate and to detect volatile compounds in a mixture. This study was aimed to compare active components of essential oils from cabinet dryer-dried nutmeg fruit flesh by water distillation and water-steam distillation. Results showed that 28 compounds were detected in essential oils of nutmeg flesh by water distillation, while 31 were identified in oil by water-steam distillation. Compounds identified with higher intensity in oil by water distillation were α-pinene (15.8%), β-pinene (12.0%), limonene (7.5%), δ-terpinene (8.7%), terpinene-4 -ol (14.4%), α-terpineol (4.9%), and myristicin (15.6%). The similar compounds were also detected in oil by water-steam distillation but in different quantities such as 13,3%, 8,8%, 7,2%, 8,7%, 15,6%, 7,7%, 13,5%, respectively. Keywords: distillation method, essential oil component, GC-MS, nutmeg fruit flesh   ABSTRAK Perkembangan industri parfum, kosmetik, farmasi, makanan dan minuman, penyedap alami, obat-obatan, aroma terapi, maupun industri bukan makanan semakin tahun semakin meningkat yang berakibat meningkatnya kebutuhan minyak atsiri. Daging buah pala menjadi salah satu potensi sumber minyak atsiri yang dapat diperoleh melalui metode distilasi (distilasi air dan distilasi air-uap). Kromatografi Gas Spectrometer Massa (GC-MS) merupakan metode yang dinamis untuk pemisahan dan deteksi senyawa-senyawa yang mudah menguap dalam suatu campuran. Tujuan penelitian ini adalah membandingkan komponen senyawa aktif minyak atsiri dari daging buah pala kering cabinet dryer yang diperoleh dari distilasi air dan distilasi air-uap menggunakan GC-MS. Hasil analisis GC-MS minyak atsiri daging buah pala yang diperoleh melalui distilasi air terdapat 28 senyawa, sedangkan dengan distilasi air-uap diperoleh 31 senyawa. Intensitas tertinggi pada metode distilasi air teridentifikasi sebagai senyawa α-pinene (15,8%), β-pinene (12,0%), limonene (7,5%), δ-terpinene (8,7%), Terpinene-4-ol (14,4%), α-terpineol (4,9%), dan myristicin (15,6%), sedangkan intensitas tertinggi pada metode distilasi air-uap teridentifikasi sebagai senyawa α-pinene (13,3%), β-pinene (8,8%), Limonene (7,2%), δ-terpinene (8,7%), terpinene-4-ol (15,6%), α-terpineol (7,7%), dan myristicin (13,5%). Kata kunci: daging buah pala, GC-MS, komponen minyak atsiri, metode distilasi


2019 ◽  
pp. 191-199 ◽  
Author(s):  
Наталья (Natal'ya) Александровна (Aleksandrovna) Коваленко (Kovalenko) ◽  
Татьяна (Tat'yana) Игоревна (Igorevna) Ахрамович (Ahramovich) ◽  
Галина (Galina) Николаевна (Nikolaevna) Супиченко (Supichenko) ◽  
Татьяна (Tat'yana) Владимировна (Vladimirovna) Сачивко (Sachivko) ◽  
Виктор (Viktor) Николаевич (Nikolaevich) Босак (Bosak)

The steam distillation method was used to obtain samples of essential oil of plants of three varieties Hyssopus officinalis L., cultivated in the conditions of the Republic of Belarus. More than 20 components were identified and determined in the essential oils of plants of the varieties Lazurit, Rozotsvetkovy, Zaveya by gas-liquid chromatography. It has been established that the studied varieties of Hyssopus officinalis belong to the pinocamphone chemotype. The main components are pinocamphone (67.6–76.0%), β-pinene, eugenol, limonene, camphene, sabinene, linalool, 1,8-cineole, α-terpineol, α-pinene, γ-terpinene and pinocampheol. The distribution of the enantiomers of a-and b-pinenes, camphene, limonene and linalool was established in the studied samples. Antimicrobial activity of essential oils and optical isomers of pure β-pinene was investigated on Staphylococcus aureus, Salmonella alony, Bacillus subtilis, Clostridium sp., Escherichia coli Hfr H, Pseudomonas aeruosa by disc method. It is shown that (+)-β-pinene showed higher antibacterial activity to (-)-β-pinene. The increased content of (+)-β-pinene in the varieties of Hyssopus officinalis with pink (Rosacea variety) and white (Zaveya variety) aureoles of flowers caused a higher antibacterial activity of their essential oils against Gram-negative and Gram-positive bacteria.


Food Research ◽  
2021 ◽  
Vol 5 (3) ◽  
pp. 29-35
Author(s):  
E.E. Mohmad Kahar ◽  
B.A. Talip ◽  
N.A. Mohd Fauzi ◽  
S.N. Kamarulzaman ◽  
F. Zakaria ◽  
...  

Agarwood is resinous valuable heartwood of Aquilaria tree resulting from self–defence mechanism towards injuries or damages exerted on the tree. Agarwood essential oil is usually extracted through a process of hydrodistillation or steam distillation which is beneficial for commercial purposes such as perfumeries and cosmetics. Hydrosol, a byproduct of the process produced in abundance and underutilized particularly in the agarwood industry. Hydrosol of agarwood is an aromatic compound that is believed to have chemical properties similar to essential oils that consist of positive antioxidant activity and positive biological activity for living cells. Several studies on properties of hydrosol of agarwood show that it contained zero nutrient but varied with minerals in the appropriate proportion that safe for human consumption. Thus, this review was aimed to discuss the agarwood hydrosol processing methods, characteristics, antioxidants properties and its comparisons to other plants hydrosol that have been commercially used as human consumption as in flavoured and non-flavoured beverages


2020 ◽  
Vol 9 (2) ◽  
pp. 54-70
Author(s):  
Milica Aćimović ◽  
Vele Tešević ◽  
Katarina Smiljanić ◽  
Mirjana Cvetković ◽  
Jovana Stanković ◽  
...  

Hydrolates, also referred to as hydrosols, floral or distillate waters, as well as aromatic waters, are produced in the same isolation process with essential oils by steam distillation. A small amount of essential oil constituents is dissolved in hydrolates providing specific organoleptic properties and flavor, as well as biological activity which makes them useful as raw material in many industries. Their popularity is still on the rise, especially in aromatherapy. The objective in this review is to analyze the chemical compositions of hydrolates and their corresponding essential oils, as well as biological activity of hydrolates (antimicrobial, antioxidant and antiinflamatory) and potential uses, not only in food industry for flavoring, and preservation of fresh-cut fruits and vegetables, but also as functional (soft) drinks. However, hydrolates can be used in aromatherapy and cosmetics, as well as in organic agriculture and aquaculture.


2020 ◽  
Vol 10 ◽  
Author(s):  
Ionara I. Dalcol ◽  
Alessandra O. Pereira ◽  
Luisa H. Paz ◽  
Gabriela Benetti ◽  
Fallon S. Siqueira ◽  
...  

Background: Aristolochia triangularis Cham., popularly known as the "cipó-mil-homens", "angelicó" and "ypê-mi", is applied for the treatment of wounds, skin diseases (6,7), digestive and circulatory system diseases as an antipyretic and for malaria fever. Objective: In this work we investigated the chemical composition, the antimicrobial and antimycobacterial activities of the essential oils (EOs) extracted from A. triangularis fresh stems and leaves collected in Southern Brazil. Methods: Fresh stems and fresh leaves of Aristolochia triangularis Cham. were separately subjected to hydrodistillation using a Clevenger-type apparatus. The chemical composition of the essential oils (EOs) were analyzed by gas chromatography/mass spectrometry (GC/MS). The oil samples were evaluated for their antimycobacterial, antibacterial and antifungal activities against twenty-four microorganisms. Results: Hydrodistillation of fresh stems and leaves of A. triangularis resulted in 0.16% (w/w) and 0.37% (w/w) respectively of light-yellow oils. Germacrene D is found in 13.2 - 13.5% in both EOs. The constituent most abundant in the stems EO (19.18%) was the oxygenated diterpene ent-Kaur-16-en-19-al (10), along with E-nerolidol (17.89%). The main constituents of the leaves EO were bicyclogermacrene (24.79%), β-elemene (11.30%), E-caryophyllene (10.40%) and germacrene A (9.42%), in addition to the previously mentioned germacrene D. The stems and leaves EOs showed capacity to inhibit the Gram-negative Enterobacter aerogenes and the stems EO capacity to inhibit Staphylococcus aureus, with MIC values of 31.2 µg/mL. S. aureus was moderately sensitive to leaves EO, while stems EO displayed moderate activity against Enterococcus faecalis and Salmonella typhimurium (MIC values of 62.5 µg/mL). Candida glabrata was highly susceptible to both EOs (MIC values < 3.9 µg/mL). The EOs showed moderate potential to inhibit the growth of Cryptococcus gatti and Cryptococcus neoformans (MICs of 62.5 μg/mL). Conclusion: The A. triangularis essential oils from stems and leaves displayed capacity to inhibit Enterobacter aerogenes (MIC values of 31.2 µg/mL) and high antifungal effect against Candida glabrata (MIC values of <3.9 µg/mL). Mycobacterium massiliense and M. abscessus were susceptible to the leaves EO, with MICs of 39.06 μg/mL. These results showed the A. triangularis essential oils potential as antifungal and antimycobacterial to be used in the development of new antibiotic.


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