scholarly journals HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6797
Author(s):  
Aniceta A. Mikulska ◽  
Dorota Filipowicz ◽  
Franciszek K. Główka ◽  
Ewelina Szczepanek-Parulska ◽  
Marek Ruchała ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Biological Fluids ◽  
Sodium Thiosulfate ◽  
Urinary Iodine ◽  
Iodine Concentration ◽  
Ion Pair ◽  
Clinical Samples ◽  
Urinary Iodine Concentration ◽  
Dihydrogen Phosphate ◽  
Validation Parameters

Iodine is an essential component for fetal neurodevelopment and maternal thyroid function. Urine iodine is the most widely used indicator of iodine status. In this study, a novel validated ion-pair HPLC–UV method was developed to measure iodine concentration in clinical samples. A sodium thiosulfate solution was added to the urine sample to convert the total free iodine to iodide. Chromatographic separation was achieved in a Pursuit XRs C8 column. The mobile phase consisted of acetonitrile and a water phase containing 18-crown-6-ether, octylamine and sodium dihydrogen phosphate. Validation parameters, such as accuracy, precision, limits of detection and quantification, linearity and stability, were determined. Urinary samples from pregnant women were used to complete the validation and confirm the method’s applicability. In the studied population of 93 pregnant women, the median UIC was lower in the group without iodine supplementation (117 µg/L, confidence interval (%CI): 95; 138) than in the supplement group (133 µg/L, %CI: 109; 157). In conclusion, the newly established ion-pair HPLC–UV method was adequately precise, accurate and fulfilled validation the criteria for analyzing compounds in biological fluids. The method is less complicated and expensive than other frequently used assays and permits the identification of the iodine-deficient subjects.

Evaluation of the urinary iodine concentration (UIC) in pregnant women using ion-pair HPLC-UV method

2021 ◽  
Author(s):  
Aniceta Mikulska ◽  
Dorota Filipowicz ◽  
Franciszek Głowka ◽  
Ewelina Szczepanek-Parulska ◽  
Marek Ruchała ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Urinary Iodine ◽  
Iodine Concentration ◽  
Ion Pair ◽  
Urinary Iodine Concentration

scholarly journals High Iodine Deficiency among Pregnant Women in Periurban Ghana: A Hospital-Based Longitudinal Study

2018 ◽  
Vol 2018 ◽  
pp. 1-5
Author(s):  
David Larbi Simpong ◽  
Yaw Asante Awuku ◽  
Kenneth Kwame Kye-Amoah ◽  
Martin Tangnaa Morna ◽  
Prince Adoba ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Pregnant Women ◽  
Iodine Deficiency ◽  
Urinary Iodine ◽  
Clinical Information ◽  
Iodine Concentration ◽  
Iodized Salt ◽  
Urinary Iodine Concentration ◽  
Study Cohort ◽  
Iodine Sufficiency

Background. Iodine deficiency causes maternal hypothyroidism which can lead to growth, cognitive, and psychomotor deficit in neonates, infants, and children. This study examined the iodine status of pregnant women in a periurban setting in Ghana. Methods. This longitudinal study recruited 125 pregnant women by purposeful convenience sampling from the antenatal clinic of the Sefwi Wiawso municipal hospital in Ghana. Urinary iodine concentration (UIC) was estimated by the ammonium persulfate method at an estimated gestational age (EGA) of 11, 20, and 32 weeks. Demographic information, iodized salt usage, and other clinical information were collected using a questionnaire. Results. The prevalence of iodine deficiency among the pregnant women was 47.2% at EGA 11 and 60.8% at both EGA of 20 and 32, whereas only 0.8% of participants not using iodized salt had iodine sufficiency at EGA 32. 18.4%, 20%, and 24% of participants using iodized salt had iodine sufficiency at EGA 11, 20, and 32, respectively. Conclusion. A high prevalence of iodine deficiency was observed among our study cohort.

scholarly journals Development and validation of an iodine-specific FFQ to estimate iodine intake in Australian pregnant women

2015 ◽  
Vol 113 (6) ◽  
pp. 944-952 ◽  
Author(s):  
Dominique Condo ◽  
Maria Makrides ◽  
Sheila Skeaff ◽  
Shao J. Zhou
Keyword(s):  
Pregnant Women ◽  
Urinary Iodine ◽  
Optimal Growth ◽  
Iodine Concentration ◽  
Iodine Intake ◽  
Urinary Iodine Excretion ◽  
Urinary Iodine Concentration ◽  
Food Record ◽  
Total Iodine ◽  
Supplement Use

Adequate iodine is important during pregnancy to ensure optimal growth and development of the offspring. We validated an iodine-specific FFQ (I-FFQ) for use in Australian pregnant women. A forty-four-item I-FFQ was developed to assess iodine intake from food and was administered to 122 pregnant women at 28 weeks gestation. Iodine supplement use was captured separately at 28 weeks gestation. Correlation between iodine intake from food estimated using the I-FFQ and a 4 d weighed food record as well as correlation between total iodine intake and 24 h urinary iodine excretion (UIE), 24 h urinary iodine concentration (UIC), spot UIC and thyroid function were assessed at 28 weeks gestation. A moderate correlation between the two dietary methods was shown (r0·349,P< 0·001), and it was strengthened with the addition of iodine supplements (r0·876,P< 0·001). There was a fair agreement (k= 0·28,P< 0·001) between the two dietary measures in the classification of women as receiving adequate ( ≥ 160 μg/d) or inadequate ( < 160 μg/d) iodine intake from food, but the limits of agreement from the Bland–Altman plot were large. Total iodine intake was associated with 24 h UIE (β = 0·488,P< 0·001) but not with spot UIC. Iodine intake from food using the I-FFQ was assessed at study entry ( < 20 weeks gestation) in addition to 28 weeks gestation, and there was a strong correlation in iodine intake at the two time points (r0·622,P< 0·001), which indicated good reproducibility. In conclusion, the I-FFQ provides a valid tool for estimating iodine intake in pregnant women and can be used to screen women who are at risk of inadequate intake.

scholarly journals Indicators of Iodine Status in Pregnancy and Postpartum in a Group of Pregnant Women from Perimarine Area of Romania

2020 ◽  
Vol 26 (2) ◽  
pp. 63-69
Author(s):  
Scrinic Olesea ◽  
Delia Corina Elena ◽  
Toma Geanina Mirela ◽  
Circo Eduard
Keyword(s):  
Pregnant Women ◽  
Iodine Deficiency ◽  
Urinary Iodine ◽  
Iodine Concentration ◽  
Iodine Intake ◽  
Iodized Salt ◽  
Urinary Iodine Concentration ◽  
Urinary Creatinine ◽  
Iodine Status ◽  
In Pregnancy

Abstract Objective: Assessment of iodine nutritional status in pregnant women in the perimarine area of Romania, a region without iodine deficiency. Adequate iodine intake is the main source for normal thyroid function, ensuring the need for maternal thyroid hormones during pregnancy, but also for the development and growth of children in the fetal and postpartum period. Material and method: Prospective study performed on 74 pregnant women in the first 2 trimesters of pregnancy, originating from the perimarin area. The following indicators of iodine status were analyzed: urinary iodine concentration (UIC), the ratio between urinary iodine concentration and urinary creatinine (UIC/UCr), the prevalence of maternal goiter and the value of neonatal TSH (thyroid stimulating hormone). Results: The mean gestational age was 11 weeks. The ways of iodine intake are: iodized salt - 59.4%, iodized salt and iodine supplements- 23%, only iodine supplements -10.8% and 6.8% consume only non-iodized salt. The median of UIC was 133.03 mcg/l considered insufficient iodine intake (normal in pregnancy UIC >150 mcg/l), but the adjustment of UIC to urinary creatinine reveals a median of 152.83 mcg/g, a value that reflects an adequate iodine intake. The prevalence of goiter was 25.6% characteristic for a moderate iodine deficiency. The prevalence of neonatal TSH >5 mIU/L was registered in 18.8% characteristic of mild iodine deficiency. Conclusions: Monitoring of the iodine nutritional status is recommended for the prevention of disorders due to iodine deficiency under the conditions of universal salt iodization. Perimarine areas considered sufficient in iodine may show variations in iodine status in subpopulations under certain physiological conditions, such as pregnancy. An indicator of iodine status of the population is UIC, but the UIC/UCr ratio may be a more optimal indicator for pregnant women, to avoid possible overestimated results of iodine deficiency in pregnancy.

scholarly journals Women Remain at Risk of Iodine Deficiency during Pregnancy: The Importance of Iodine Supplementation before Conception and Throughout Gestation

Nutrients ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 172 ◽  
Author(s):  
Kristen Hynes ◽  
Judy Seal ◽  
Petr Otahal ◽  
Wendy Oddy ◽  
John Burgess
Keyword(s):  
At Risk ◽  
Pregnant Women ◽  
Iodine Deficiency ◽  
Urinary Iodine ◽  
Iodine Concentration ◽  
Urinary Iodine Concentration ◽  
Iodine Nutrition ◽  
Supplement Use ◽  
Iodine Supplementation ◽  
The Impact

In Australia, pregnant women are advised to take an iodine supplement (I-supp) (150 µg/day) to reduce risks to the foetus associated with iodine deficiency (ID). To examine the impact of this recommendation on iodine status, and to identify factors that contribute to adequacy during gestation, supplement use and Urinary Iodine Concentration (UIC) was measured in 255 pregnant women (gestation range 6 to 41 weeks) in Tasmania. The median UIC (MUIC) of 133 µg/L (Inter-quartile range 82–233) was indicative of ID, being below the 150–249 µg/L range for adequacy during pregnancy. Women taking an iodine-containing-supplement (I-supp) had a significantly higher MUIC (155 µg/L) (n = 171) compared to the combined MUIC (112.5 µg/L) (n = 84) of those who had never (120 µg/L) (n = 61) or were no longer taking an I-supp (90 µg/L) (n = 23) (p = 0.017). Among women reporting I-supp use, the MUIC of those commencing the recommended 150 µg/day prior to conception was significantly higher than those starting supplementation following pregnancy confirmation: 196 (98–315) µg/L (n = 45) versus 137.5 (82.5–233.5) µg/L (n = 124), p = 0.032. Despite recommendations for iodine supplementation pregnant Tasmanian women remain at risk of ID. Commencing an I-supp of 150 µg/day prior to conception and continuing throughout pregnancy is required to ensure adequacy. Timely advice regarding the importance of adequate iodine nutrition, including supplementation is needed to reduce the risk of irreversible in utero neurocognitive damage to the foetus.

scholarly journals Iodine nutrition status in clinically euthyroid pregnant women attending in BSMMU

2016 ◽  
Vol 9 (1) ◽  
pp. 38
Author(s):  
Md. Anowar Hossain ◽  
M. Abul Hasanat ◽  
Murshed Ahamed Khan ◽  
Jobaida Naznin ◽  
Kazi Ashraful Alam ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Urinary Iodine ◽  
Iodine Content ◽  
Iodine Concentration ◽  
Nutrition Status ◽  
Urinary Iodine Concentration ◽  
Digestion Method ◽  
Iodine Nutrition ◽  
Cross Sectional ◽  
Nutritional Problem

<p><strong>Background:</strong> Iodine deficiency disorders (IDD) are common nutritional problem globally. All groups of people are affected by it, but the pregnant women and their neonates are most vulnerable.</p><p><strong>Objectives:</strong> This study was carried out to see the iodine status of pregnant women using median urinary iodine concentration (MUI) as a measure of outcome. Methods: This cross sectional observational study assessed the MUI in casual urine samples from 225 pregnant women (75 pregnants from each trimester) and 75 non-pregnant healthy controls. The urinary iodine content was estimated in urine sample using the method of Dunn et al. with the modification of Sandell &amp; Kolthoff (wet digestion method).</p><p><strong>Results:</strong> Median value of urinary iodine in pregnant women was 48.21Ug/L compared to 52.27</p>

Hypertension and Salt-Restrictive Diet Promotes Low Urinary Iodine Concentration in High-Risk Pregnant Women: Results from a Cross-Sectional Study Conducted After Salt Iodination Reduction in Brazil

2020 ◽  
Vol 197 (2) ◽  
pp. 445-453 ◽  
Author(s):  
Luciana Sant’Ana Leone de Souza ◽  
Renata de Oliveira Campos ◽  
Vitor dos Santos Alves ◽  
Taíse Lima Oliveira Cerqueira ◽  
Thiago Magalhães da Silva ◽  
...  
Keyword(s):  
High Risk ◽  
Pregnant Women ◽  
Urinary Iodine ◽  
Iodine Concentration ◽  
Cross Sectional Study ◽  
Urinary Iodine Concentration ◽  
Sectional Study ◽  
Cross Sectional ◽  
Restrictive Diet

scholarly journals The impact of iodised salt or iodine supplements on iodine status during pregnancy, lactation and infancy

2007 ◽  
Vol 10 (12A) ◽  
pp. 1584-1595 ◽  
Author(s):  
Michael B Zimmermann
Keyword(s):  
Pregnant Women ◽  
Urinary Iodine ◽  
Iodine Concentration ◽  
Iodine Intake ◽  
Urinary Iodine Concentration ◽  
Life Stages ◽  
Lactating Women ◽  
Iodine Status ◽  
Iodised Salt ◽  
The Impact

AbstractObjectives:Monitoring of iodine status during pregnancy, lactation and infancy is difficult as there are no established reference criteria for urinary iodine concentration (UI) for these groups; so it is uncertain whether iodized salt programs meet the needs of these life stages.Design and Subjects:The method used in this paper was: 1) to estimate the median UI concentration that reflects adequate iodine intake during these life stages; and 2) to use these estimates in a review of the literature to assess whether salt iodisation can control iodine deficiency in pregnant and lactating women, and their infants.Results:For pregnancy, recommended mean daily iodine intakes of 220-250 μg were estimated to correspond to a median UI concentration of about 150 μg l− 1, and larger surveys from the iodine sufficient countries have reported a median UI in pregnant women ≥ 140 μg l− 1. Iodine supplementation in pregnant women who are mild-to-moderately iodine deficient is beneficial, but there is no clear affect on maternal or newborn thyroid hormone levels. In countries where the iodine intake is sufficient, most mothers have median breast milk iodine concentration (BMIC) greater than the concentration (100-120 μg l− 1) required to meet an infant's needs. The median UI concentration during infancy that indicates optimal iodine nutrition is estimated to be ≥ 100 μg l− 1. In iodine-sufficient countries, the median UI concentration in infants ranges from 90-170 μg l− 1, suggesting adequate iodine intake in infancy.Conclusions:These findings suggest pregnant and lactating women and their infants in countries with successful sustained iodised salt programs have adequate iodine status.

Sign in / Sign up

Export Citation Format

Share Document