Molecular identification and antimicrobial activities of some wild Egyptian mushrooms: Bjerkandera adusta as a promising source of bioactive antimicrobial phenolic compounds

Abstract Background The discovery of potential, new cost-effective drug resources in the form of bioactive compounds from mushrooms is one way to control the resistant pathogens. In the present research, the fruiting bodies of five wild mushrooms were collected from Egypt and identified using internal transcribed spacer region (ITS) of the rRNA encoding gene and their phylogenetic relationships, antimicrobial activities, and biochemical and phenolic compounds were evaluated. Results The sequences revealed identity to Bjerkandera adusta, Cyclocybe cylindracea, Agrocybe aegerita, Chlorophyllum molybdites, and Lentinus squarrosulus in which Cyclocybe cylindracea and Agrocybe aegerita were closely related, while Chlorophyllum molybdites was far distant. Cyclocybe cylindracea and Agrocybe aegerita showed 100% similarity based on the sequenced ITS-rDNA fragment and dissimilar antimicrobial activities and chemical composition were detected. Bjerkandera adusta and Cyclocybe cylindracea showed strong antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Micrococcus luteus, Streptococcus pneumoniae, and Candida albicans. This activity could be attributed to the detected phenolic and related compounds’ contents. Conclusion Our finding provides a quick and robust implement for mushroom identification that would facilitate mushroom domestication and characterization for human benefit.

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Comparative Evaluation of Chemical Composition, Phenolic Compounds, and Antioxidant and Antimicrobial Activities of Tropical Black Bolete Mushroom Using Different Preservation Methods

Foods ◽

10.3390/foods10040781 ◽

2021 ◽

Vol 10 (4) ◽

pp. 781

Author(s):

Jaturong Kumla ◽

Nakarin Suwannarach ◽

Keerati Tanruean ◽

Saisamorn Lumyong

Keyword(s):

Chemical Composition ◽

Phenolic Compounds ◽

Natural Antioxidants ◽

Antimicrobial Activities ◽

Fresh Sample ◽

Antioxidant Power ◽

Preservation Methods ◽

Ferric Reducing Antioxidant Power ◽

Nutritional Benefits ◽

Antioxidant And Antimicrobial Activities

Tropical black bolete, Phlebopus portentosus, provides various nutritional benefits and natural antioxidants to humans. In this study, the chemical composition, phenolic compounds, and antioxidant and antimicrobial activities of fresh mushroom samples and samples stored for a period of one year using different preservation methods (drying, brining, and frozen) were investigated. The results indicated that the brining method significantly reduced the protein and fat contents of the mushrooms. The polyphenol and flavonoid contents of the frozen sample were not significantly different from that of the fresh sample. The results revealed that an inhibition value of 50% (IC50) for the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay of the extract of the dried and frozen samples was not statistically different from that of the fresh sample. The IC50 value of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay and ferric reducing antioxidant power (FRAP) value in the extract of the frozen sample were not found to be significantly different from those of the fresh sample. Furthermore, the lowest degree of antioxidant activity was found in the extract of the brined sample. Additionally, the antimicrobial activities of the extracts of the fresh and frozen samples were not significantly different and both extracts could have inhibited the growth of all tested Gram-positive bacteria and Pseudomonas aeruginosa.

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Biological Properties of Essential Oils from Thymus algeriensis Boiss

Plants ◽

10.3390/plants10040786 ◽

2021 ◽

Vol 10 (4) ◽

pp. 786

Author(s):

Hamza Ouakouak ◽

Adel Benarfa ◽

Mohammed Messaoudi ◽

Samir Begaa ◽

Barbara Sawicka ◽

...

Keyword(s):

Essential Oils ◽

Micrococcus Luteus ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Cancer Cell Line ◽

Antimicrobial Activities ◽

Biological Properties ◽

Chemical Components ◽

Bornyl Acetate ◽

Thymus Algeriensis

This study describes the chemical composition, antitumor, antioxidant, and antimicrobial activities of the plant Thymus algeriensis Boiss. Essential oils (EOs) were collected in different periods (before, during, and after flowering stage) from the El-Guetfa region, M’sila, Algeria. The EOs extraction was achieved using three distinguishing techniques: hydro (Clevenger trap), steam, and microwave distillations, targeting different aerial parts of the plant (stems, flowers, and leaves). The EOs chemical components were estimated using GC-FID and GC-MS apparatuses. The resulting yield of the extracted oil was moderate and ranged between 0.84 and 1.53% (wt/vol). In total, eighty-five components were identified, in which the oxygenated monoterpenes family formed the main portion, starting from 40.56 up to 70.66%. The obtained essential oil was dominated by five major components that varied from low to quite moderate percentages: camphor (17.45–32.56%), borneol (11.16–22.2%), camphene (7.53–12.86%), 1.8-cineole (5.16–11.21%), and bornyl acetate (3.86–7.92%). The biological results of this oil pointed out that the EOs extracted from the leaves part exposed a weak radical scavenging activity afterward using two well-known antioxidant assays DPPH (IC50 = 8.37 mg/mL) and ABTS (10.84 mg/mL). Meanwhile, this oil presented strong inhibition activity against colon cancer cell line HCT116 (LC50 = 39.8 µg/mL) and a moderate inhibitory against hepatocellular cancer cells HePG2 (LC50 > 100 µg/mL). In addition, this oil antimicrobial activity was quite important against Micrococcus luteus (M. luteus), Staphylococcus aureus CIP 7625, Escherichia coli ATCC 10536, Saccharomyces cerevisiae ATCC 4226, Candida albicans IPA200, Candida tropicalis (Ct), and Candida glabrata (Cg) after using Amoxicillin and Itraconazole as references.

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Antioxidant properties, quantification and stability of betalains from pitaya (Hylocereus undatus) peel

Ciência Rural ◽

10.1590/0103-8478cr20140548 ◽

2014 ◽

Vol 45 (2) ◽

pp. 323-328 ◽

Cited By ~ 12

Author(s):

Fernanda Robert de Mello ◽

Claudia Bernardo ◽

Caroline Odebrecht Dias ◽

Luciano Gonzaga ◽

Edna Regina Amante ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Antioxidant Properties ◽

Raw Material ◽

High Concentration ◽

Natural Colorant ◽

Wide Ph Range ◽

Hylocereus Undatus ◽

Ph Range ◽

Promising Source

Pitaya peel can be used as a raw material for betalains extraction. The aim of this research was to quantify phenolic compounds, antioxidant activity and betalains on pitaya peel. Furthermore, evaluate the betalains stability against various pH conditions and exposure time of heating. The results showed that pitaya peel contains phenolic compounds and presented antioxidant activity. Moreover it showed high concentration of betalains (101.04mg equivalent to betanin. 100g-1) which were stable over a wide pH range (3.2 - 7.0) and were resistant to heating (100oC) up to 10 minutes at pH range from 3.7 to 5.5. Therefore, pitaya peel is a promising source of betalains which can be applied as a natural colorant for food.

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Antimicrobial Activities of Mefloquine and a Series of Related Compounds

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.44.4.848-852.2000 ◽

2000 ◽

Vol 44 (4) ◽

pp. 848-852 ◽

Cited By ~ 55

Author(s):

C. M. Kunin ◽

W. Y. Ellis

Keyword(s):

Staphylococcus Epidermidis ◽

Antimicrobial Activities ◽

Walter Reed Army Institute ◽

Gram Negative Bacteria ◽

Antimicrobial Drugs ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Resistant Strains ◽

Vancomycin Resistant ◽

Related Compounds

ABSTRACT Mefloquine was found to have bactericidal activity against methicillin- and fluoroquinolone-susceptible and -resistant strains ofStaphylococcus aureus and Staphylococcus epidermidis and gentamicin- and vancomycin-resistant strains ofEnterococcus faecalis and Enterococcus faecium. The MICs were 16 μg/ml, and the minimal bactericidal concentrations (MBCs) were 16 to 32 μg/ml. These concentrations cannot be achieved in serum. Mefloquine was active at a more achievable concentration against penicillin-susceptible and -resistant Streptococcus pneumoniae, with MICs of 0.2 to 1.5 μg/ml. Mefloquine was not active against gram-negative bacteria and yeasts. In an attempt to find more active derivatives, 400 mefloquine-related compounds were selected from the chemical inventory of The Walter Reed Army Institute of Research. We identified a series of compounds containing a piperidine methanol group attached to pyridine, quinoline, and benzylquinoline ring systems. These had activities similar to that of mefloquine against S. pneumoniae but were far more active against other gram-positive bacteria (MICs for staphylococci, 0.8 to 6.3 μg/ml). They had activities similar to that of amphotericin B againstCandida spp. and Cryptococcus neoformans. Combinations of the compounds with gentamicin and vancomycin were additive against staphylococci and pneumococci. The MIC and MBC of gentamicin were decreased by four- to eightfold when this drug was combined with limiting dilutions of the compounds. There was no antagonism with other antimicrobial drugs. The compounds were rapidly bactericidal. They appear to act by disrupting cell membranes. Combinations of the compounds with aminoglycoside antibiotics may have potential for therapeutic use.

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Enhanced Efficiency of the Removal of Cytostatic Anthracycline Drugs Using Immobilized Mycelium of Bjerkandera adusta CCBAS 930

Molecules ◽

10.3390/molecules26226842 ◽

2021 ◽

Vol 26 (22) ◽

pp. 6842

Author(s):

Kamila Rybczyńska-Tkaczyk

Keyword(s):

Superoxide Dismutase ◽

Free Radicals ◽

Phenolic Compounds ◽

Glucose Oxidase ◽

Biological Treatment ◽

Lepidium Sativum ◽

Bjerkandera Adusta ◽

Anthracycline Antibiotics ◽

Lepidium Sativum L

The aim of this study was to evaluate the bioremoval of anthracycline antibiotics (daunomycin-DNR, doxorubicin–DOX, and mitoxantrone-MTX) by immobilized mycelium of B. adusta CCBAS 930. The activity of oxidoreductases: versatile peroxidases (VP), superoxide dismutase (SOD), catalase (CAT), and glucose oxidase (GOX), and the levels of phenolic compounds (PhC) and free radicals (SOR) were determined during the biotransformation of anthracyclines by B. adusta strain CCBAS 930. Moreover, the phytotoxicity (Lepidium sativum L.), biotoxicity (MARA assay), and genotoxicity of anthracyclines were evaluated after biological treatment. After 120 h, more than 90% of anthracyclines were removed by the immobilized mycelium of B. adusta CCBAS 930. The effective biotransformation of anthracyclines was correlated with detoxification and reduced genotoxicity.

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Biochemical Analysis of Phenolics Compounds Accumulated During Developing Stages of Wheatgrass

International Journal for Research in Applied Sciences and Biotechnology ◽

10.31033/ijrasb.8.2.22 ◽

2021 ◽

Vol 8 (2) ◽

pp. 167-172

Author(s):

Nikita Nimsarkar ◽

Mayuri Kulkarni ◽

Dr. Faiyaz Shaikh

Keyword(s):

Phenolic Compounds ◽

Qualitative Analysis ◽

Biochemical Analysis ◽

Developmental Stages ◽

Methanol Extract ◽

Standard Condition ◽

Antimicrobial Activities ◽

Future Perspective ◽

Biochemical Tests ◽

Phenolics Compounds

The current study was carried out to evaluate the accumulation of phenolic compounds in distinct developmental stages of Wheatgrass (Tritium aestivum). The wheatgrass was grown in the container/tray in the standard condition in the laboratory space provided by the department. Overnight soaked seeds were then sowed in the soil containers for 25 days. The accumulation of phenolics compounds was qualitatively and quantitatively checked in the leaves at 5, 10,15,20,25 days. Phenolics compounds were sequentially extracted in methanol, n-hexane, and acetone. For qualitative analysis, various biochemical tests were carried out and phenolics compounds were quantitatively estimated by Folin-Ciocalteu reagent. Among three solvents methanol extract was found to accumulate phenolics compounds in increasing concentration at various developmental stages i.e. 5th days - 0.7135 mg/ml, 10th days 1.2614 mg/ml, 15th days- 1.1244 mg/ml; 20th days - 1.8093 mg/ml and 25th days - 4.0693 mg/ml. On the qualitative check, methanol extract was found to be positive for tannins, terpenoids, and glycosides. These phenolics could be analyzed for antidiabetic and antimicrobial activities are the future perspective of this study.

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Bacterial Remediation of Phenolic Compounds

Biotechnology ◽

10.4018/978-1-5225-8903-7.ch078 ◽

2019 ◽

pp. 1910-1943

Author(s):

Veena Gayathri Krishnaswamy

Keyword(s):

Phenolic Compounds ◽

Industrial Revolution ◽

Marine Ecosystem ◽

Research Work ◽

Cost Effective ◽

Real Problem ◽

Comprehensive Information ◽

Treatment Technologies ◽

Biological Methods ◽

Micro Organisms

Environmental pollution has been an irrefutable fact of life for many centuries; but it has become a real problem, since the start of the industrial revolution. Discharge of these toxic compounds without treatment results in serious health risks to humans and the marine ecosystem. Several physical, chemical and biological methods have been employed for the remediation of the phenolics. Bioremediation is identified as the most efficient, cost effective and eco-friendly ways for treatment of phenolic compounds. This article is a comprehensive review on the sources of phenolic compounds, their hazards, and their fate once released into the environment; the treatment technologies employed and bioremediation of these compounds using both non-extremophlic and extremophilic organisms. The review, throws light on the enzymes involved in the remediation of phenolic compounds, highlights the importance of extremophilic organisms and biological treatment of phenol containing industrial wastewaters. Such comprehensive information on the research work performed for the remediation of phenolic compounds provide ways to explore the role played by micro organisms in the remediation of phenolic compounds, which could be applied in the remediation of phenol /contaminated sites even under extreme conditions.

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Exogenous Production of Silver Nanoparticles by Tephrosia apollinea Living Plants under Drought Stress and Their Antimicrobial Activities

Nanomaterials ◽

10.3390/nano9121716 ◽

2019 ◽

Vol 9 (12) ◽

pp. 1716 ◽

Cited By ~ 1

Author(s):

Muna Ali ◽

Kareem Mosa ◽

Ali El-Keblawy ◽

Hussain Alawadhi

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Drought Stress ◽

Visible Spectrum ◽

Cost Effective ◽

Antimicrobial Activities ◽

Living Plant ◽

X Ray ◽

Toxicological Effect ◽

Tephrosia Apollinea

Nanoparticle (NP) synthesis by biological systems is more cost-effective, safe, and environmentally friendly when compared to currently used chemical and physical methods. Although many studies have utilized different plant extracts to synthesize NPs, few studies have incorporated living plants. In this study, silver nanoparticles (AgNPs) were synthesized exogenously by Tephrosia apollinea living plant system under the combined stresses of silver nitrate and different levels of drought stress simulated by Polyethylene glycol (PEG) (0, −0.1, −0.2, and −0.4 MPa for three and six days). Biomass, cell death, and H2O2 content were evaluated to determine the toxicological effect of the treatments on the plant. More severe effects were detected in day 6 plants compared to day 3 plants, and at higher drought levels. UV-visible spectrum, energy dispersive X-ray spectroscopy, X-ray diffraction, scanning electron microscope, and Fourier transform infrared spectroscopy were used to detect and characterize the T. apollinea synthesized NPs. The shapes of the NPs were spherical and cubic with different phytochemicals being the possible capping agents. Broth microdilution was used to determine the antimicrobial activity of the NPs against Escherichia coli and Staphylococcus aureus. In this case, antimicrobial activity increased at higher PEG concentrations. Bactericidal effects were observed against E. coli, while only bacteriostatic effects were detected against S. aureus.

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Biodecolorization of anthraquinone dyes using immobilised mycelium of Bjerkandera adusta CCBAS930

E3S Web of Conferences ◽

10.1051/e3sconf/202017101013 ◽

2020 ◽

Vol 171 ◽

pp. 01013 ◽

Cited By ~ 1

Author(s):

Kamila Rybczyńska-Tkaczyk ◽

Teresa Korniłłowicz-Kowalska

Keyword(s):

Phenolic Compounds ◽

Lepidium Sativum ◽

Carminic Acid ◽

Secondary Products ◽

Bjerkandera Adusta ◽

Anthraquinone Dyes ◽

Before And After ◽

Acid Blue 129 ◽

Acid Blue ◽

Acid Green 25

The aim of this study was to characterize the activity of oxidoreductases during biotransformation of 0.01% anthraquinone dyes: Alizarin Blue Black B (ABBB) and Acid Blue 129 (AB129), Carminic Acid (CA), Remazol Brilliant Blue R (RBBR), Acid Green 25 (AG25) and Poly R-478 by immobilized strain of Bjerkandera adusta CCBAS 930. Phenolic compounds, phytotoxicity (Lepidium sativum L.), biotoxicity were evaluated to determine the toxicity of anthraquinone dyes before and after the treatment with immobilized B. adusta CCBAS 930. More than 60% of CA and AB129 were removed by immobilized B. adusta CCBAS after 7 days. No secondary products toxic to plants and bacteria were formed during immobilized cultures of B. adusta CCBAS 930.

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Color for Life: Biosynthesis and Distribution of Phenolic Compounds in Pepper (Capsicum annuum)

Agriculture ◽

10.3390/agriculture9040081 ◽

2019 ◽

Vol 9 (4) ◽

pp. 81 ◽

Cited By ~ 5

Author(s):

Carvalho Lemos ◽

Reimer ◽

Wormit

Keyword(s):

Phenolic Compounds ◽

Capsicum Annuum ◽

Fruits And Vegetables ◽

Current Knowledge ◽

Industrial Applications ◽

Special Focus ◽

Residual Biomass ◽

New Concepts ◽

Health Related ◽

Related Compounds

Fruits and vegetables are an important supplier of biological active substances, such as vitamins and secondary metabolites (SM) for human nutrition, but also for further industrial applications. Pepper (Capsicum annuum) is grown and consumed all over the world as a fresh vegetable or dried as a spice. It is also used as a coloring agent, as well for medical purposes. Pepper fruits are considered as an attractive source of health-related compounds, but other organs like the leaves and stem also contain considerable amounts of antioxidants, e.g., phenolic compounds. This indicates potential for valorization of residual biomass from horticultural production by using innovative bioeconomic concepts. Herein, we present an overview about the biosynthesis of phenolic compounds, with a special focus on flavonoids and their regulation in pepper, the current knowledge of amounts and distribution of these valuable substances, as well as possible strategies for: (1) increasing flavonoid contents in pepper, (2) improving the nutritional value of fruits, and (3) new concepts for utilization of residual biomass from horticultural production.

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