scholarly journals Effects of Zn-Doped Mesoporous Bioactive Glass Nanoparticles in Etch-and-Rinse Adhesive on the Microtensile Bond Strength

Nanomaterials ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1943 ◽  
Author(s):  
Yeonju Choi ◽  
Woogyeong Sun ◽  
Yeon Kim ◽  
In-Ryoung Kim ◽  
Mi-Kyung Gong ◽  
...  
Keyword(s):  
Bond Strength ◽  
Bioactive Glass ◽  
Biological Effects ◽  
Biological Properties ◽  
Microtensile Bond Strength ◽  
Alizarin Red ◽  
Etch And Rinse ◽  
Before And After ◽  
The Matrix ◽  
Mesoporous Bioactive Glass

The purpose of this study was to assess the effects in the dentin bond strength of dental adhesives (DAs) and biological effects using zinc (Zn)-doped mesoporous bioactive glass nanoparticles (MBN-Zn). Synthesized MBN and MBN-Zn were characterized by scanning electron microscopy (SEM), X-ray diffraction and the Brunauer, Emmett and Teller (BET) method. The matrix metalloproteinases (MMP) inhibition effects of DA-MBN and DA-MBN-Zn were analyzed. The microtensile bond strength (MTBS) test was conducted before and after thermocycling to investigate the effects of MBN and MBN-Zn on the MTBS of DAs. The biological properties of DA-MBN and DA-MBN-Zn were analyzed with human dental pulp stem cells (hDPSCs). Compared with the DA, only the DA-1.0% MBN and DA-1.0% MBN-Zn exhibited a statistically significant decrease in MMP activity. The MTBS values after thermocycling were significantly increased in DA-1.0% MBN and DA-1.0% MBN-Zn compared with the DA (p < 0.05). It was confirmed via the MTT assay that there was no cytotoxicity for hDPSCs at 50% extract. In addition, significant increases in the alkaline phosphatase activity and Alizarin Red S staining were observed only in DA-1.0%MBN-Zn. These data suggest the 1.0% MBN and 1.0% MBN-Zn enhance the remineralization capability of DAs and stabilize the long-term MTBS of DAs by inhibiting MMPs.

scholarly journals Adhesives Doped with Bioactive Niobophosphate Micro-Filler: Degree of Conversion and Microtensile Bond Strength

2016 ◽  
Vol 27 (6) ◽  
pp. 705-711 ◽  
Author(s):  
Karina Kato Carneiro ◽  
Marcia Margarete Meier ◽  
Clenilton Costa dos Santos ◽  
Adeilton Pereira Maciel ◽  
Ceci Nunes Carvalho ◽  
...  
Keyword(s):  
Bond Strength ◽  
Bioactive Glass ◽  
Adhesive System ◽  
Degree Of Conversion ◽  
Adhesive Systems ◽  
Microtensile Bond Strength ◽  
Glass Addition ◽  
Etch And Rinse ◽  
One Step ◽  
Niobium Phosphate

Abstract To evaluate the effect of incorporating niobium phosphate bioactive glass (NbG) into commercial etch-and-rinse adhesive systems, with and without silane, on their degree of conversion (DC) (%) and microtensile bond strength (μTBS). The NbG micro-filler was added to two etch-and-rinse adhesive systems: One Step (OS) and Prime & Bond (PB) at 40% concentration. The following groups were formed: control without glass addition OS; addition of unsilanized NbG (OSNbG); addition of silanized NbG (OSNbGS); control without glass PB; addition of unsilanized NbG (PBNbG); addition of silanized NbG (PBNbGS). The DC was determined using total Fourier spectroscopy reflection (FTIR/ATR). For μTBS testing, 48 human third molars (n=8) were restored and sliced to obtain specimens (0.8 mm2) and they were tested at two different time intervals: immediately and after 6 months. The fracture mode was evaluated with a stereoscopic loupe (40×) and by scanning electron microscopy (SEM). The data were subjected to ANOVA and Tukey tests (a=0.05). NbG addition did not compromise the adhesive system DC values (p>0.05). Furthermore, the NbG added to the adhesive systems did not affect μTBS values (p>0.05). Fracture occurred predominantly at the dentin-adhesive interface. NbG bioactive glass did not affect the DC or microtensile bond strength results.

scholarly journals Anti-Microbial and Remineralizing Properties of Self-Adhesive Orthodontic Resin Containing Mesoporous Bioactive Glass

Materials ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3550
Author(s):  
Aerin Choi ◽  
Kyung-Hyeon Yoo ◽  
Seog-Young Yoon ◽  
Bong-Soo Park ◽  
In-Ryoung Kim ◽  
...  
Keyword(s):  
Bond Strength ◽  
Physical Properties ◽  
Bioactive Glass ◽  
Shear Bond Strength ◽  
Antibacterial Effect ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Significant Difference ◽  
Mesoporous Bioactive Glass

Self-adhesive resins (SARs) contain adhesives, which simplify the procedures of resin application, and primers, which provide sufficient bonding ability. In this study, mesoporous bioactive glass nanoparticles (MBN) were added to a SAR to easily improve the physical properties and remineralization ability. The experimental resins comprised 1%, 3%, and 5% MBN mixed in Ortho Connect Flow (GC Corp, Tokyo, Japan). As the MBN content in the SAR increased, the microhardness increased, and a statistically significant difference was observed between the cases of 1% and 5% MBN addition. Shear bond strength increased for 1% and 3% MBN samples and decreased for 5% MBN. The addition of MBN indicated a statistically significant antibacterial effect on both gram-negative and gram-positive bacteria. The anti-demineralization experiment showed that the remineralization length increased with the MBN content of the sample. Through the above results, we found that SAR containing MBN has antibacterial and remineralization effects. Thus, by adding MBN to the SAR, we investigated the possibility of orthodontic resin development, wherein the strength is enhanced and the drawbacks of the conventional SAR addressed.

scholarly journals Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems

Marine Drugs ◽  
2020 ◽  
Vol 18 (5) ◽  
pp. 263 ◽  
Author(s):  
Eugenia Baena ◽  
Sandra R Cunha ◽  
Tatjana Maravić ◽  
Allegra Comba ◽  
Federica Paganelli ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Bond Strength ◽  
Adhesive Systems ◽  
Microtensile Bond Strength ◽  
Bond Stability ◽  
Etch And Rinse ◽  
Mechanical Durability ◽  
Human Dentine ◽  
Self Etch ◽  
Metalloproteinase Activity

The aim of the present study was to evaluate the effect of 0.1% chitosan (Ch) solution as an additional primer on the mechanical durability and enzymatic activity on dentine using an etch-and-rinse (E&R) adhesive and a universal self-etch (SE) adhesive. Microtensile bond strength and interfacial nanoleakage expression of the bonded interfaces for all adhesives (with or without pretreatment with 0.1% Ch solution for 1 min and air-dried for 5 s) were analyzed immediately and after 10,000 thermocycles. Zymograms of protein extracts from human dentine powder incubated with Optibond FL and Scotchbond Universal on untreated or Ch-treated dentine were obtained to examine dentine matrix metalloproteinase (MMP) activities. The use of 0.1% Ch solution as an additional primer in conjunction with the E&R or SE adhesive did not appear to have influenced the immediate bond strength (T0) or bond strength after thermocycling (T1). Zymography showed a reduction in MMP activities only for mineralized and demineralized dentine powder after the application of Ch. Application of 0.1% Ch solution does not increase the longevity of resin–dentine bonds. Nonetheless, the procedure appears to be proficient in reducing dentine MMP activities within groups without adhesive treatments. Further studies are required to comprehend the cross-linking of Ch with dentine collagen.

Bond Strength of Two-Step Etch-and-Rinse Adhesive Systems to the Dentin of Primary and Permanent Teeth

2010 ◽  
Vol 35 (2) ◽  
pp. 163-168 ◽  
Author(s):  
Hérica Adad Ricci ◽  
Mariane Emi Sanabe ◽  
Carlos Alberto de Souza Costa ◽  
Josimeri Hebling
Keyword(s):  
Bond Strength ◽  
Adhesive System ◽  
Permanent Tooth ◽  
Permanent Teeth ◽  
Adhesive Systems ◽  
Microtensile Bond Strength ◽  
Human Teeth ◽  
Cross Sectional ◽  
Etch And Rinse ◽  
The Mean

Objective: The purpose of this study was to compare the immediate microtensile bond strength (µTBS) of two-step etch-and-rinse adhesive systems to the dentin of primary and permanent teeth. Study Design: Non-carious human teeth (12 primary molars and 12 premolars) were assigned to 3 groups according to the adhesive system. The adhesive systems were applied to flat superficial coronal dentin surfaces etched with phosphoric acid and composite resin blocks were built up. The teeth were sectioned to produce beam-shaped specimens with 0.81 mm2 cross-sectional area subjected to µTBS testing. µTBS data were analyzed statistically by ANOVA and Tukey’s test (a= 0.05). Results: The adhesive systems produced statistically similar mean µTBS to each other (p&gt;0.05) and no significant differences (p&gt;0.05) were found when the same material was applied to primary or permanent tooth dentin. The mean µTBS values (MPa) obtained were: Prime& Bond NT: 41.7±14.4 (permanent) and 40.8±13.4 (primary); Single Bond: 42.9±8.6 (permanent) and 41.4±11.9 (primary); Excite DSC: 46.3±11.3 (permanent teeth) and 43.4±12.0 (primary). Conclusion:There was no difference in the immediate µTBS of two-step etch-and-rinse adhesive systems when applied to the dentin of primary and permanent teeth.

scholarly journals Effect of Two Antioxidants Agents on Microtensile Bond Strength to Bleached Enamel

2016 ◽  
Vol 27 (5) ◽  
pp. 532-536 ◽  
Author(s):  
Marina Studart Alencar ◽  
◽  
Juliana Fraga Soares Bombonatti ◽  
Rafael Massunari Maenosono ◽  
Ana Flávia Soares ◽  
...  
Keyword(s):  
Bond Strength ◽  
Sodium Bicarbonate ◽  
Artificial Saliva ◽  
Sodium Bicarbonate Solution ◽  
Sodium Ascorbate ◽  
Scanning Electron Microscopy Data ◽  
Microtensile Bond Strength ◽  
Etch And Rinse ◽  
Bovine Enamel

Abstract This in vitro study evaluated the effect of sodium bicarbonate and sodium ascorbate on the microtensile bond strength of an etch-and-rinse system to bleached bovine enamel. Sixty bovine enamel blocks (4x4 mm) were flattened and randomly allocated into 5 groups: G1 (negative control): without treatment; G2 (positive control): bleached with 35% hydrogen peroxide (HP); G3: bleached and stored for 7 days in artificial saliva before restorative procedures; G4: bleached and treated with 10% sodium bicarbonate solution for 5 min; G5: bleached and treated with 10% sodium ascorbate hydrogel for 15 min. HP gel was applied twice (20 min each, except in G1) and the adhesive restorations were performed. After 24 h, the specimens were sectioned into sticks and submitted to microtensile bond strength testing with a crosshead speed of 0.5 mm/min (n=12). As a complementary visual observation, the enamel surfaces of the G1 and G2 specimens were evaluated with scanning electron microscopy. Data were analyzed by one-way ANOVA (p<0.05). The means (standard deviation) were: G1: 24.22±7.74; G2: 18.29±5.88; G3: 40.88±7.95; G4: 19.95±5.67 and G5: 24.43±6.43. Adhesive failures were predominant in all groups. The comparison between the treatments indicates that waiting 7 days after bleaching is still the most effective approach. When this waiting period is not possible, application of sodium ascorbate or sodium bicarbonate seems to be a good alternative. Therefore, the practicality of obtaining sodium bicarbonate in the bleaching kits and its higher stability enables its clinical use.

scholarly journals Chitosan in different concentrations added to a two-step etch-and-rinse adhesive system: influence on bond strength to dentin

2017 ◽  
Vol 20 (4) ◽  
pp. 55
Author(s):  
Rafael Avellar de Carvalho Nunes ◽  
Flávia Lucisano Botelho do Amaral ◽  
Fabiana Mantovani Gomes França ◽  
Cecilia Pedroso Turssi ◽  
Roberta Tarkany Basting
Keyword(s):  
Bond Strength ◽  
Failure Mode ◽  
Failure Modes ◽  
Resin Composite ◽  
Adhesive System ◽  
Fracture Pattern ◽  
Test Machine ◽  
Microtensile Bond Strength ◽  
Etch And Rinse ◽  
Strength Tests

<p class="Corpo"><strong>Objective</strong>: the aim of the present study was to evaluate the influence of adding different concentrations of chitosan to an experimental two-step etch-and-rinse adhesive system on the bond strength and failure mode to dentin. <strong>Material</strong> <strong>and</strong> <strong>Methods</strong>: thirty-two flat dentin surfaces were obtained from extracted human third molars and divided into four groups  (n=8) for application of the adhesive systems: AD - conventional two-step adhesive system (Adper Single Bond 2); EXP – experimental two-step etch-and-rinse adhesive system; Chi0.2% - EXP with addition of 0.2% Chitosan; Chi0.5% - EXP with addition of 0.5% Chitosan. Resin composite build-ups were made and the composite/dentin specimens were sectioned to obtain rectangular beams with a bond area of approximately 1mm<sup>2</sup>. After 24 hours, the sticks were submitted to microtensile bond strength tests in a universal test machine. The fracture pattern was evaluated under a stereoscopic loupe at 40X magnification. <strong>Results</strong>: one-way analysis of variance showed that the type of adhesive system had no significant effect on the bond strength values (p = 0.142), showing the mean bond strength values (standard deviation), in MPa, for the groups as follows: AD=20.1 (5.4); EXP=16.6 (2.3); Chi0.2%=16.1 (2.8); Chi0.5%=16.9 (2.3). In all the groups there was predominance of cohesive fractures in dentin, representing 68 to 82% of the failure modes. <strong>Conclusion</strong>: the addition of 0.2 or 0.5% of chitosan had no influence on the bond strength and failure mode of an experimental two-step etch-and-rinse adhesive system to dentin.</p><p class="Corpo"><strong>Keywords</strong></p><p class="Corpo">Chitosan; Dental Adhesives; Failure Mode; Microtensile Bond Strength.</p>

scholarly journals The Effect of Mesoporous Bioactive Glass Nanoparticles/Graphene Oxide Composites on the Differentiation and Mineralization of Human Dental Pulp Stem Cells

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 620 ◽  
Author(s):  
Jae Hwa Ahn ◽  
In-Ryoung Kim ◽  
Yeon Kim ◽  
Dong-Hyun Kim ◽  
Soo-Byung Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Bioactive Glass ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Mrna Levels ◽  
Alizarin Red ◽  
Differentiation Potential ◽  
Human Dental Pulp ◽  
Mesoporous Bioactive Glass

The purpose of this study was to investigate the effects of mesoporous bioactive glass nanoparticle (MBN)/graphene oxide (GO) composites on the mineralization ability and differentiation potential of human dental pulp stem cells (hDPSCs). MBN/GO composites were synthesized using the sol-gel method and colloidal processing to enhance the bioactivity and mechanical properties of MBN. Characterization using FESEM, XRD, FTIR, and Raman spectrometry showed that the composites were successfully synthesized. hDPSCs were then cultured directly on the MBN/GO (40:1 and 20:1) composites in vitro. MBN/GO promoted the proliferation and alkaline phosphatase (ALP) activity of hDPSCs. In addition, qRT-PCR showed that MBN/GO regulated the mRNA levels of odontogenic markers (dentin sialophosphoprotein (DSPP), dentine matrix protein 1 (DMP-1), ALP, matrix extracellular phosphoglycoprotein (MEPE), bone morphogenetic protein 2 (BMP-2), and runt-related transcription factor 2 (RUNX-2)). The mRNA levels of DSPP and DMP-1, two odontogenesis-specific markers, were considerably upregulated in hDPSCs in response to growth on the MBN/GO composites. Western blot analysis revealed similar results. Alizarin red S staining was subsequently performed to further investigate MBN/GO-induced mineralization of hDPSCs. It was revealed that MBN/GO composites promote odontogenic differentiation via the Wnt/β-catenin signaling pathway. Collectively, the results of the present study suggest that MBN/GO composites may promote the differentiation of hDPSCs into odontoblast-like cells, and potentially induce dentin formation.

Effect of Different Adhesive Strategies and Time on Microtensile Bond Strength of a CAD/CAM Composite to Dentin

2019 ◽  
Vol 44 (3) ◽  
pp. 262-272
Author(s):  
EM Meda ◽  
RN Rached ◽  
SA Ignácio ◽  
IA Fornazari ◽  
EM Souza
Keyword(s):  
Bond Strength ◽  
Computer Aided Design ◽  
Optical Microscope ◽  
Single Bond ◽  
Microtensile Bond Strength ◽  
Resin Cements ◽  
Bonding Agents ◽  
Etch And Rinse ◽  
Computer Aided ◽  
Cad Cam

SUMMARY Purpose: The aim of this study was to evaluate the effect of adhesive strategy and time on the microtensile bond strength of a computer-aided design/computer-aided manufacturing (CAD/CAM) composite to dentin. Methods and Materials: Sixty CAD/CAM composite blocks were bonded to human dentin with simplified bonding agents using etch-and-rinse and self-etching approaches and amine-based and amine-free resin cements, with and without the application of a dual-cure activator (DCA; n=10): SBP-ARC (Adper Single Bond Plus + RelyX ARC), SBP-RXU (Adper Single Bond Plus + RelyX Ultimate), SBP-DCA-RXU (Adper Single Bond Plus + DCA + RelyX Ultimate), SBU-ARC (Scotchbond Universal + RelyX ARC), SBU-RXU (Scotchbond Universal + RelyX Ultimate), and SBU-DCA-ARC (Scotchbond Universal + DCA + RelyX ARC). Each specimen was light cured for 40 seconds under load and stored in distilled water at 37°C for seven days. Stick-shaped specimens (1.0 mm2) were obtained. Half of the specimens underwent microtensile bond strength testing, and the other half were subjected to the same tests after six months of storage. Failure mode was determined using an optical microscope (40×). The data were analyzed by a two-way analysis of variance followed by the Games-Howell test and Student t-test (preset alpha of 0.05). Results: After seven days, SBU-RXU presented the highest mean bond strength, statistically different from only SBU-ARC (p&lt;0.05). Most of the groups exhibited a statistically significant reduction in bond strength after 6 months (p&lt;0.05), except SBP-RXU and SBU-ARC (p&gt;0.05). Conclusion: The adhesive strategy, with different associations between adhesive systems and resin cements, as well as the use of a DCA, affected the bond strength of both amine-free and amine-based resin cements to a CAD/CAM composite.

Sign in / Sign up

Export Citation Format

Share Document