scholarly journals Bilayered Fibrin-Based Electrospun-Sprayed Scaffold Loaded with Platelet Lysate Enhances Wound Healing in a Diabetic Mouse Model

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2128
Author(s):  
Paola Losi ◽  
Tamer Al Kayal ◽  
Marianna Buscemi ◽  
Ilenia Foffa ◽  
Aida Cavallo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Growth Factor ◽  
Phase Inversion ◽  
Diabetic Mouse ◽  
Platelet Lysate ◽  
Inversion Method ◽  
Post Surgery ◽  
Phase Inversion Method

The present study examined the effects of a bilayered fibrin/poly(ether)urethane scaffold loaded with platelet lysate by a combination of electrospinning and spray, phase-inversion method for wound healing. In particular, the poly(ether)urethane layer was obtained using by a spray phase-inversion method and the fibrin fibers network were loaded with platelet lysate by electrospinning. The kinetics release and the bioactivity of growth factors released from platelet lysate-scaffold were investigated by ELISA and cell proliferation test using mouse fibroblasts, respectively. The in-vitro experiments demonstrated that a bilayered fibrin/poly(ether)urethane scaffold loaded with platelet lysate provides a sustained release of bioactive platelet-derived growth factors. The effect of a bilayered fibrin/poly(ether)urethane scaffold loaded with platelet lysate on wound healing in diabetic mouse (db/db) was also investigated. The application of the scaffold on full-thickness skin wounds significantly accelerated wound closure at day 14 post-surgery when compared to scaffold without platelet lysates or commercially available polyurethane film, and at the same level of growth factor-loaded scaffold. Histological analysis demonstrated an increased re-epithelialization and collagen deposition in platelet lysate and growth factor loaded scaffolds. The ability of bilayered fibrin/poly(ether)urethane scaffold loaded with platelet lysate to promote in-vivo wound healing suggests its usefulness in clinical treatment of diabetic ulcers.

scholarly journals PEG grafted chitosan scaffold for dual growth factor delivery for enhanced wound healing

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Amritha Vijayan ◽  
Sabareeswaran A. ◽  
G. S. Vinod Kumar
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Growth Factor ◽  
Treated Group ◽  
Growth Factor Delivery ◽  
Chitosan Scaffold ◽  
Collagen Formation ◽  
Dual Growth Factor Delivery

AbstractApplication of growth factors at wound site has improved the efficiency and quality of healing. Basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) induce proliferation of various cells in wound healing. Delivery of growth factor from controlled release systems protect it from degradation and also result in sustained delivery of it at the site of injury. The goal of the study was to develop a Polyethylene glycol (PEG) cross-linked cotton-like chitosan scaffold (CS-PEG-H) by freeze-drying method and chemically conjugate heparin to the scaffold to which the growth factors can be electrostatically bound and evaluate its wound healing properties in vitro and in vivo. The growth factor containing scaffolds induced increased proliferation of HaCaT cells, increased neovascularization and collagen formation seen by H and E and Masson’s trichrome staining. Immunohistochemistry was performed using the Ki67 marker which increased proliferation of cells in growth factor containing scaffold treated group. Frequent dressing changes are a major deterrent to proper wound healing. Our system was found to release both VEGF and bFGF in a continuous manner and attained stability after 7 days. Thus our system can maintain therapeutic levels of growth factor at the wound bed thereby avoiding the need for daily applications and frequent dressing changes. Thus, it can be a promising candidate for wound healing.

Angiogenic Growth Factors: Their Effects and Potential in Soft Tissue Wound Healing

1992 ◽  
Vol 101 (4) ◽  
pp. 349-354 ◽  
Author(s):  
David B. Hom ◽  
Robert H. Maisel
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Growth Factor ◽  
Soft Tissue ◽  
Transforming Growth Factor Beta ◽  
Animal Studies ◽  
Transforming Growth Factor ◽  
Therapeutic Potential ◽  
Angiogenic Growth Factors

Since their discovery 30 years ago, angiogenic growth factors have been demonstrated to stimulate neovascularization in vitro and in animal studies. Over the last decade, knowledge gained in the field of angiogenic growth factors has grown immensely. These angiogenic growth factors exist in four major families: fibroblast growth factor (FGF), transforming growth factor beta (TGF-β), platelet-derived growth factor (PDGF), and epidermal growth factor (EGF). Each has the ability to induce soft tissue vascularization in microgram quantities. In animal models, FGF, TGF-β, PDGF, and EGF have been shown to enhance soft tissue wound healing. In human clinical trials, EGF and a mixture of PDGFs have been demonstrated to accelerate epidermal regeneration in cutaneous wounds. These factors have considerable therapeutic potential in the areas of soft tissue wound healing and otolaryngology. This article reviews important aspects of angiogenic growth factors and discusses their future potential in soft tissue wound healing.

scholarly journals Preparation, Characterization, and In Vitro Hemocompatibility of Glutaraldehyde-Crosslinked Chitosan/Carboxymethylcellulose as Hemodialysis Membrane

2021 ◽  
Vol 21 (5) ◽  
pp. 1120
Author(s):  
Khabibi Khabibi ◽  
Dwi Siswanta ◽  
Mudasir Mudasir
Keyword(s):  
Mechanical Strength ◽  
Protein Adsorption ◽  
Partial Thromboplastin Time ◽  
Carboxymethyl Cellulose ◽  
Phase Inversion ◽  
Inversion Method ◽  
Crosslinked Chitosan ◽  
Phase Inversion Method ◽  
Hemodialysis Membrane

This study aims to examine the manufacture, characterization, and in vitro hemocompatibility of glutaraldehyde-crosslinked chitosan/carboxymethyl cellulose (CS/CMC-GA) as a hemodialysis membrane. The CS/CMC-GA membrane was prepared using the phase inversion method with 1.5% CS and 0.1% CMC. The chitosan was crosslinked with glutaraldehyde in various monomers ratios, and the membranes formed were characterized by FTIR, SEM, and TGA. Furthermore, the hydrophilicity, swelling, porosity, mechanical strength, and dialysis performance of the membranes against urea and creatinine were systematically examined, and their in-vitro hemocompatibility tests were also conducted. The results showed that the CS/CMC-GA membranes have higher hydrophilicity, swelling, porosity, mechanical strength, and better dialysis performance against urea and creatinine than chitosan without modification. In addition, the hemocompatibility test indicated that the CS/CMC-GA membranes have lower values of protein adsorption, thrombocyte attachment, hemolysis ratio, and partial thromboplastin time (PTT) than that of pristine chitosan. Based on these results, the CC/CMC-GA membranes have better hemocompatibility and the potential to be used as hemodialysis membranes.

scholarly journals Laminin heparin-binding peptides promiscuously bind growth factors and enhance diabetic wound healing

2018 ◽  
Author(s):  
Jun Ishihara ◽  
Ako Ishihara ◽  
Kazuto Fukunaga ◽  
Priscilla S. Briquez ◽  
Jeffrey A. Hubbell
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Growth Factor ◽  
Vascular Endothelial Cell ◽  
Diabetic Mouse ◽  
Heparin Binding ◽  
Surface Receptors ◽  
Binding Domains ◽  
Skin Healing

AbstractLaminin, as a key component of the basement membrane extracellular matrix (ECM), regulates tissue morphogenesis. We show that multiple laminin isoforms promiscuously bind to growth factors (GFs) with high affinity, through their heparin binding domains (HBDs) located in the a chain LG domains. Interestingly, these domains also bind to syndecan cell-surface receptors, promoting attachment of fibroblasts and endothelial cells. We next explore application of these multifunctional laminin HBDs in skin healing in the type 2 diabetic mouse. We demonstrate that covalent incorporation of laminin HBDs into fibrin matrix enables the slow-release of GFs. Incorporation of the α33043-3067 laminin HBD significantly enhances in vivo wound-healing efficacy of vascular endothelial cell growth factor (VEGF)-A165 and platelet-derived growth factor (PDGF)-BB, under conditions where the GFs alone in fibrin are inefficacious. This laminin HBD peptide may be clinically useful by improving biomaterials as both GF reservoirs and cell scaffolds, leading to effective tissue regeneration.

A straight, open and macro-porous fuel electrode-supported protonic ceramic electrochemical cell

2021 ◽  
Author(s):  
Yuxin Pan ◽  
Kai Pei ◽  
Yucun Zhou ◽  
Tong Liu ◽  
Meilin Liu ◽  
...  
Keyword(s):  
Phase Inversion ◽  
Electrochemical Cell ◽  
Inversion Method ◽  
Phase Inversion Method

A straight, open and macro-porous Ni–BaZr0.1Ce0.7Y0.1Yb0.1O3 fuel electrode-supported protonic ceramic electrochemical cell has been fabricated by a modified phase-inversion method.

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