scholarly journals Preparation of Lignin Nanoparticles from Wood Waste for Wood Surface Treatment

Nanomaterials ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 281 ◽  
Author(s):  
Florian Zikeli ◽  
Vittorio Vinciguerra ◽  
Alessandro D’Annibale ◽  
Donatella Capitani ◽  
Manuela Romagnoli ◽  
...  
Keyword(s):  
Surface Treatment ◽  
Wood Surface ◽  
Dip Coating ◽  
Size Exclusion ◽  
Artificial Weathering ◽  
Before And After ◽  
Exclusion Chromatography ◽  
Heteronuclear Single Quantum Correlation ◽  
Protection Potential ◽  
Dip Coating Technique

Lignin was isolated from wood wastes comprising Iroko sawdust (IR) and mixed sawdust from Iroko and Norway spruce (IRNS), furnished by a local wood houses producer. The respective acidolysis lignin fractions were structurally characterized using pyrolysis (Py)-GCMS, two-dimensional heteronuclear single quantum correlation nuclear magnetic resonance (2D HSQC NMR), Fourier-transform infrared FTIR and ultraviolet-visible (UV-VIS) spectroscopies, size exclusion chromatography, and standard wet-chemistry methods for Klason lignin and polysaccharides determination. The isolated lignin fractions were subsequently used for the preparation of lignin nanoparticles (LNPs) using a non-solvent method. LNPs were then used for wood surface treatment using a dip-coating technique. The coated wood samples were analyzed by colorimetry and scanning electron microscopy (SEM) before and after artificial weathering experiments in a UV chamber to investigate the UV protection potential of the LNPs coatings. Wood samples dip-coated with LNPs showed promising surface modifications resembling a sort of film of fused LNPs. Coatings made from IR-LNPs and IRNS-LNPs performed significantly better in artificial weathering experiments than uncoated reference samples.

scholarly journals Identification of food-derived peptides in human blood after ingestion of corn and wheat gluten hydrolysates

2018 ◽  
Vol 2 ◽  
Author(s):  
Akika Ejima ◽  
Megumi Nakamura ◽  
Yasushi A. Suzuki ◽  
Kenji Sato
Keyword(s):  
Matrix Protein ◽  
Leucine Aminopeptidase ◽  
Wheat Gluten ◽  
Protein Hydrolysates ◽  
The Body ◽  
Size Exclusion ◽  
Extracellular Matrix Protein ◽  
Reverse Phase Hplc ◽  
Before And After ◽  
Exclusion Chromatography

Bioactive peptides in the body after ingestion of plant protein hydrolysates have been speculated but not identified. We aimed to establish an approach to identify small amounts of food-derived peptides in humans after ingestion of non-extracellular matrix protein hydrolysates. Corn and wheat gluten hydrolysates were digested using pancreatin and leucine aminopeptidase; the resultant peptides were identified via size-exclusion chromatography and reverse-phase HPLC-tandem mass spectrometry (MS/MS). Structures of indigestible peptides were confirmed via LC-MS/MS in multi-reaction monitoring mode. All indigestible peptides in the exopeptidase digest were diprolyl and di- and tripyroglutamyl peptides. Blood collected from healthy volunteers (n = 4) before and after ingestion of 9 g of the hydrolysates was assessed for the indigestible peptides via LC-MS/MS. Six peptides (Pro-Ala, Pro-Gly, Pro-Gln, pyroGlu-Pro, pyroGlu-Leu-Pro, and pyroGlu-Gln-Pro) significantly increased in human plasma up to 10–100 nM compared to the baseline. This may hence be a powerful tool for identifying foodderived peptides in blood.

In vitro digestibility study of thermal oxidized palm oleins Estudio de la digestibilidad in vitro de oleínas de palma termooxidadas

2000 ◽  
Vol 6 (6) ◽  
pp. 449-456 ◽  
Author(s):  
F.J. Sánchez-Muniz ◽  
R. Arroyo ◽  
J.M. Sánchez-Montero ◽  
C. Cuesta
Keyword(s):  
Pancreatic Lipase ◽  
High Performance ◽  
Palm Olein ◽  
Size Exclusion ◽  
In Vitro Digestibility ◽  
Moderate Degree ◽  
Before And After ◽  
Exclusion Chromatography ◽  
Hydrolysis Of

Information on digestibility and absorption of oils and fats used for frying is under debate. To get knowledge on this, unused palm olein (9.27 ± 0.10% w/w polar content), used frying palm olein with a moderate degree of alteration (14.81 ± 0.90% w/w polar content) and highly altered used frying palm olein (26.36 ± 0.30% w/w polar content) and their respective nonpolar and polar fractions were studied. Samples were analyzed by high-performance size-exclusion chromatography before and after a 20-min in vitro incubation with pancreatic lipase. Formation of monoacylglycerols and free fatty acids reflected no relevant differences between unused and moderately altered oleins, whereas the most altered olein was hydrolyzed to a much lesser degree. The presence of oligomers (dimers and polymers of triacylglycerols) negatively affected the hydrolysis of triacylglycerol monomers in whole oleins. The hydrolysis of these monomers in the isolated nonpolar and polar fractions ranked between 60.2% and 78.5%. Oligomers were efficiently hydrolyzed by pancreatic lipase in whole un used and moderately altered oleins but not in the most altered one. Polymers from isolated polar fractions were poorly hydrolyzed or not hydrolyzed at all. These data suggest that whole oleins contained some compounds that increase susceptibility of oligomers to enzymatic hydrolysis and that such compounds were not present in the polar fraction.

scholarly journals Sol-gel derived coatings for the conservation of steel

2015 ◽  
Vol 9 (2) ◽  
pp. 81-89 ◽  
Author(s):  
Erika Kiele ◽  
Jurate Senvaitiene ◽  
Asta Grigucevicienė ◽  
Rimantas Ramanauskas ◽  
Rimantas Raudonis ◽  
...  
Keyword(s):  
Sol Gel ◽  
Dip Coating ◽  
Processing Route ◽  
Force Microscopy ◽  
Angle Measurements ◽  
Before And After ◽  
Silica Sols ◽  
Commercial Polymers ◽  
Dip Coating Technique ◽  
Steel Substrates

In this paper, sol-gel processing route has been applied and investigated for the conservation of steel. Nanosilica coatings on steel surface have been prepared using tetraethylorthosilicate (TEOS) as a starting material. The methyl-modified silica sols were obtained by mixing of 3mas.% SiO2 sol solution with hexamethyldisilozane (HMDS). The surface of steel was coated by dip-coating technique. In order to compare the characteristics of coatings, the steel substrates were also coated with commercial polymers Paraloid B67, Cosmolloid H80 and Antik Patina. The surface morphology changes of the uncoated and coated specimens before and after photochemical ageing were investigated by scanning electron microscopy and atomic force microscopy. The structure of the prepared coatings was also investigated by FTIR spectroscopy. The hydrophobicity of surfaces was evaluated by contact angle measurements. Potentiodynamic measurements were obtained in order to compare corrosion parameters of the coatings.

Purification Method of Silver Nanoparticles (AgNPs) and its Identification Using UV-Vis Spectrophotometer

2020 ◽  
Vol 840 ◽  
pp. 484-491
Author(s):  
Umi Nur Sholikhah ◽  
Deni Pranowo ◽  
Rizky Ibnufaatih Arvianto ◽  
Endang Sarmini ◽  
Triani Widyaningrum
Keyword(s):  
Silver Nanoparticles ◽  
Size Exclusion Chromatography ◽  
Chemical Reduction ◽  
Size Exclusion ◽  
Chemical Reduction Method ◽  
Purification Method ◽  
Resonance Characteristic ◽  
Before And After ◽  
Exclusion Chromatography ◽  
Centrifugation Method

The development of nanotechnology applications is rapidly growing in many sectors. One of them is silver nanoparticles (AgNPs) which are metal nanoparticles that play an important role, especially in nanomedicine. The most effective method of purifying to obtain stable AgNPs is very important to study. Experiments on the separation of AgNPs have been carried out using the size exclusion chromatography and centrifugation methods to see the effectiveness of refining the two methods. This experiment begins with the synthesis of AgNPs using the chemical reduction method. Then, the synthesized AgNPs were purified by Size Exclusion Chromatography (SEC) and centrifugation method then analyzed using UV-Vis spectrophotometer to determine the maximum peaks before and after purification. The experimental results were obtained that centrifugation methods and SEC having the same effectiveness in refining AgNPs. The centrifugation method at various speed (0, 3000, 6000, 9000, 12000 and 15000 rpm) gave wavelength results 403, 404, 404, 405, 404, and 404 nm. The SEC method using Sephadex-25 column showed the 4th to 8th fractions gave the maximum wavelength 404, 404, 404, 405, and 404 nm, respectively. The maximum wavelength of both methods showed the surface plasmon resonance characteristic of AgNPs. However, centrifugation at 3000 rpm has better homogeneity than SEC method.

Influence of the homehold composting conditions on the structural changes of polylactide spun-bonded nonwovens during degradation

2016 ◽  
Vol 87 (20) ◽  
pp. 2541-2549 ◽  
Author(s):  
Michał Puchalski ◽  
Piotr Siwek ◽  
Tadeusz Biela ◽  
Sławomir Sztajnowski ◽  
Michał Chrzanowski ◽  
...  
Keyword(s):  
Supramolecular Structure ◽  
Structural Changes ◽  
Physical And Mechanical Properties ◽  
Size Exclusion ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
Before And After ◽  
Exclusion Chromatography ◽  
Chicken Litter ◽  
Multiangle Laser Light Scattering

In this study, the influence of homehold composting conditions on the molecular and supramolecular structure of polylactide (PLA) in the form of spun-bonded nonwovens was investigated. Nonwoven samples were studied using size-exclusion chromatography coupled with multiangle laser light scattering detection, wide-angle X-ray diffraction, differential scanning calorimetry and Fourier transform infrared spectroscopy. In addition, the physical and mechanical properties of the nonwovens before and after composting were determined. The results show the varying degree of influence of the prepared compost mixtures of soil with common horticultural additives, such as chalk, commercially available agents, cow manure and chicken litter, on the molecular and supramolecular structure of PLA and its degradation rate. The obtained experiment explained which popular homehold agent had the strongest affect on the PLA nonwoven dedicated for agriculture use in the first period of season composting (first 6 months).

Impact of extracted algogenic organic matter on coagulation performance

2015 ◽  
Vol 15 (3) ◽  
pp. 617-624 ◽  
Author(s):  
Linan Xing ◽  
Christopher W. K. Chow ◽  
Jiane Zuo ◽  
Dongsheng Wang ◽  
Rolando Fabris ◽  
...  
Keyword(s):  
Organic Matter ◽  
Algal Blooms ◽  
High Performance ◽  
Three Dimensional ◽  
Product Formation ◽  
Size Exclusion ◽  
Uv Absorbance ◽  
Fluorescence Excitation ◽  
Before And After ◽  
Exclusion Chromatography

Understanding coagulation behaviour and treatability of waters impacted by algogenic organic matter (AOM) is important for waters with frequent algal blooms. Physico­­–chemical characteristics of AOM spiked into a water sample, before and after coagulation, were investigated using high-performance size exclusion chromatography (HPSEC) with UV and fluorescence detection, three dimensional-fluorescence excitation emission matrix (3D-FEEM) measurement and resin fractionation in which three fractions were determined including very hydrophobic acid (VHA), slightly hydrophobic acid (SHA) and hydrophilic fractions. Release of AOM from algal cells with consequential increases in dissolved organic carbon and UV absorbance led to changes in 3D-FEEM spectra indicative of increased aromatic protein presence. Changes in disinfection by-product formation potential after the AOM spiking indicated possible interactions between natural organic matter and AOM. A study of the treatability of the AOM spiked water using two coagulants, alum and a polyaluminum composite coagulant, was conducted with the relative percentages of UV absorbance values of both the SHA and hydrophilic fractions higher in the post coagulated AOM spiked water than in the coagulated water, with corresponding reductions in the VHA proportion. It was found that the increased SHA and hydrophilic components in the AOM spiked natural water were recalcitrant to removal by both coagulants.

CHARACTERIZATION OF A NEW LCAT MUTATION CAUSING FAMILIAL LCAT DEFICIENCY (FLD) AND THE ROLE OF APOE AS A MODIFIER GENE OF THE FLD PHENOTYPE

2009 ◽  
Vol 32 (6S) ◽  
pp. 3
Author(s):  
A Baass ◽  
H Wassef ◽  
M Tremblay ◽  
L Bernier ◽  
R Dufour ◽  
...  
Keyword(s):  
Modifier Gene ◽  
Size Exclusion ◽  
Plasma Lipoproteins ◽  
Lipoprotein Profile ◽  
Exclusion Chromatography ◽  
Low Hdl ◽  
Lcat Deficiency ◽  
Apoe Genotypes

Introduction: LCAT (lecithin:cholesterol acyltransferase ) is an enzyme which plays an essential role in cholesterol esterification and reverse cholesterol transport. Familial LCAT deficiency (FLD) is a disease characterized by a defect in LCAT resulting in extremely low HDL-C, premature corneal opacities, anemia as well as proteinuria and renal failure. Method: We have identified two brothers presenting characteristics of familial LCAT deficiency. We sequenced the LCAT gene, measured the lipid profile as well as the LCAT activity in 15 members of this kindred. We also characterized the plasma lipoproteins by agarose gel electrophoresis and size exclusion chromatography and sequenced several candidate genes related to dysbetalipoproteinemia in this family. Results: We have identified the first French Canadian kindred with familial LCAT deficiency. Two brothers affected by FLD, were homozygous for a novel LCAT mutation. This c.102delG mutation occurs at the codon for His35 causing a frameshift that stops transcription at codon 61 abolishing LCAT enzymatic activity both in vivo and in vitro. It has a dramatic effect on the lipoprotein profile, with an important reduction of HDL-C in both heterozygotes (22%) and homozygotes (88%) and a significant decrease in LDL-C in heterozygotes (35%) as well as homozygotes (58%). Furthermore, the lipoprotein profile differed markedly between the two affected brothers who had different APOE genotypes. We propose that APOE could be an important modifier gene explaining heterogeneity in lipoprotein profiles observed among FLD patients. Our results suggest that a LCAT-/- genotype associated with an APOE ?2 allele could be a novel mechanism leading to dysbetalipoproteinemia.

Structure of a shear-thickening polysaccharide extracted from the New Zealand black tree fern, Cyathea medullaris

2020 ◽  
Author(s):  
M Wee ◽  
M Mastrangelo ◽  
Susan Carnachan ◽  
Ian Sims ◽  
K Goh
Keyword(s):  
New Zealand ◽  
Uronic Acid ◽  
Average Molecular Weight ◽  
Shear Thickening ◽  
Water Soluble ◽  
Size Exclusion ◽  
Resonance Spectroscopy ◽  
Tree Fern ◽  
13C Nuclear Magnetic Resonance ◽  
Exclusion Chromatography

A shear-thickening water-soluble polysaccharide was purified from mucilage extracted from the fronds of the New Zealand black tree fern (Cyathea medullaris or 'mamaku' in Māori) and its structure characterised. Constituent sugar analysis by three complementary methods, combined with linkage analysis (of carboxyl reduced samples) and 1H and 13C nuclear magnetic resonance spectroscopy (NMR) revealed a glucuronomannan comprising a backbone of 4-linked methylesterified glucopyranosyl uronic acid and 2-linked mannopyranosyl residues, branched at O-3 of 45% and at both O-3 and O-4 of 53% of the mannopyranosyl residues with side chains likely comprising terminal xylopyranosyl, terminal galactopyranosyl, non-methylesterified terminal glucopyranosyl uronic acid and 3-linked glucopyranosyl uronic acid residues. The weight-average molecular weight of the purified polysaccharide was ~1.9×106Da as determined by size-exclusion chromatography coupled with multi-angle laser light scattering (SEC-MALLS). The distinctive rheological properties of this polysaccharide are discussed in relation to its structure. © 2014 Elsevier B.V.

Sign in / Sign up

Export Citation Format

Share Document