scholarly journals Stabilization of Vitamin D in Pea Protein Isolate Nanoemulsions Increases Its Bioefficacy in Rats

Nutrients ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 75 ◽  
Author(s):  
Ali M. Almajwal ◽  
Mahmoud M. A. Abulmeaty ◽  
Hao Feng ◽  
Nawaf W. Alruwaili ◽  
Astrid Dominguez-Uscanga ◽  
...  
Keyword(s):  
Vitamin D ◽  
Protein Isolate ◽  
Normal Diet ◽  
Control Group ◽  
Albino Rats ◽  
Deficient Diet ◽  
Pea Protein ◽  
Hydroxyvitamin D ◽  
Serum Pth ◽  
Pea Protein Isolate

Micronutrient delivery formulations based on nanoemulsions can enhance the absorption of nutrients and bioactives, and thus, are of great potential for food fortification and supplementation strategies. The aim was to evaluate the bioefficacy of vitamin D (VitD) encapsulated in nanoemulsions developed by sonication and pH-shifting of pea protein isolate (PPI) in restoring VitD status in VitD-deficient rats. Weaned male albino rats (n = 35) were fed either normal diet AIN-93G (VitD 1000 IU/kg) (control group; n = 7) or a VitD-deficient diet (<50 IU/kg) for six weeks (VitD-deficient group; n = 28). VitD-deficient rats were divided into four subgroups (n = 7/group). Nano-VitD and Oil-VitD groups received a dose of VitD (81 µg) dispersed in either PPI-nanoemulsions or in canola oil, respectively, every other day for one week. Their control groups, Nano-control and Oil-control, received the respective delivery vehicles without VitD. Serum 25-hydroxyvitamin D [25(OH)VitD], parathyroid hormone (PTH), Ca, P, and alkaline phosphatase (ALP) activity were measured. After one week of treatment, the VitD-deficient rats consuming Nano-VitD recovered from Vitamin D deficiency (VDD) as compared against baseline and had serum 25(OH)VitD higher than the Nano-control. Enhancement in VitD status was followed with expected changes in serum PTH, Ca, P, and ALP levels, as compared against the controls. Stabilization of VitD within PPI-based nanoemulsions enhances its absorption and restores its status and biomarkers of bone resorption in VitD-deficient rats.

scholarly journals Stabilization of Vitamin D in Pea Protein Isolate Nanoemulsions Increases Its Bioefficacy in Rats

2018 ◽  
Author(s):  
Ali M. Almajwal ◽  
Mahmoud M.A. Abulmeaty ◽  
Hao Feng ◽  
Nawaf W. Alruwaili ◽  
Astrid Dominguez-Uscanga ◽  
...  
Keyword(s):  
Vitamin D ◽  
Protein Isolate ◽  
Albino Rats ◽  
Control Groups ◽  
Deficient Diet ◽  
Pea Protein ◽  
Hydroxyvitamin D ◽  
Ph Shifting ◽  
Serum Pth ◽  
Pea Protein Isolate

1) Background: The aim was to evaluate the bioefficacy of vitamin D (VitD) encapsulated in nanoemulsions developed by sonication and pH-shifting of pea protein isolate in restoring VitD status in VitD-deficient rats. 2) Methods: Weaned (3-week old), male albino rats (n = 35) were initially divided into two groups: control sufficient group (VDS; n = 7) fed on a normal AIN-93G diet and a VitD-deficient group (n = 28) fed a VitD-deficient diet for six weeks. VitD-deficient rats were divided into four subgroups: two treatment groups (Nano+VitD and Oil+VitD) and two control groups (Nano-VitD and Oil-VitD), receiving seven rats in each sub-group. Nano+VitD and Oil+VitD groups received VitD dispersed in PPI-nanoemulsions and in canola oil, respectively, while the control groups received the respective delivery vehicles without VitD. Serum 25-hydroxyvitamin D [25(OH)VitD], parathyroid hormone (PTH), calcium (Ca), phosphorus levels (P), and alkaline phosphatase (ALP) activity were measured. Femur bone was used to prepare histopathological sections. 3) Results: After one week of treatment, the VitD-deficient rats consuming Nano+VitD recovered from VitD deficiency (serum 25(OH)VitD 34.38 &plusmn; 7.00) compared to the sufficient control (36.84 &plusmn; 9.16; p &gt; 0.05) and the deficient control consuming VitD+Oil (14.05 &plusmn; 3.08; p &lt; 0.01). Enhancement in VitD status was followed with expected changes in serum PTH, Ca, P, and ALP levels, no difference in similar biomarkers against the sufficient control, and an improvement of the osteoid area and reduction of trabecular separation in bone. 4) Conclusions: Stabilization of VitD within PPI-based nanoemulsions enhances its absorption and restores its status and biomarkers of bone resorption in VitD-deficient rats.

scholarly journals A Novel Rat Model of Vitamin D Deficiency: Safe and Rapid Induction of Vitamin D and Calcitriol Deficiency without Hyperparathyroidism

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Andrea W. D. Stavenuiter ◽  
Maria Vittoria Arcidiacono ◽  
Evelina Ferrantelli ◽  
Eelco D. Keuning ◽  
Marc Vila Cuenca ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Hypovitaminosis D ◽  
Vitamin D Metabolites ◽  
Deficient Diet ◽  
Hydroxyvitamin D ◽  
Dihydroxyvitamin D ◽  
Study Results ◽  
Wide Range ◽  
Serum Pth

Vitamin D deficiency is associated with a range of clinical disorders. To study the mechanisms involved and improve treatments, animal models are tremendously useful. Current vitamin D deficient rat models have important practical limitations, including time requirements when using, exclusively, a vitamin D deficient diet. More importantly, induction of hypovitaminosis D causes significant fluctuations in parathyroid hormone (PTH) and mineral levels, complicating the interpretation of study results. To overcome these shortcomings, we report the successful induction of vitamin D deficiency within three weeks, with stable serum PTH and minerals levels, in Wistar rats. We incorporated two additional manoeuvres compared to a conventional diet. Firstly, the vitamin D depleted diet is calcium (Ca) enriched, to attenuate the development of secondary hyperparathyroidism. Secondly, six intraperitoneal injections of paricalcitol during the first two weeks are given to induce the rapid degradation of circulating vitamin D metabolites. After three weeks, serum 25-hydroxyvitamin D3(25D) and 1,25-dihydroxyvitamin D3(1,25D) levels had dropped below detection limits, with unchanged serum PTH, Ca, and phosphate (P) levels. Therefore, this model provides a useful tool to examine the sole effect of hypovitaminosis D, in a wide range of research settings, without confounding changes in PTH, Ca, and P.

scholarly journals Investigating the effect of paricalcitol on serum FGF23 in vitamin D deficient rats

2019 ◽  
Author(s):  
Forough Saki ◽  
Gholamhossein Ranjbar Omrani ◽  
Farhad Koohpeyma
Keyword(s):  
Vitamin D ◽  
Laboratory Data ◽  
Fractional Excretion ◽  
Male Rats ◽  
Control Group ◽  
Deficient Diet ◽  
Study Results ◽  
Significant Difference ◽  
Serum Biochemical ◽  
Serum Pth

Abstract Background: We conducted this study to discover changes of serum FGF23 in non-uremic rat model of vitamin D deficiency without secondary hyperparathyroidism using paricalcitol. Methods: 30 adult male rats weighting 300±20 grams were enrolled. They were divided into three groups of 10 rats including Control, Vitamin D deficient(VDD), and Vitamin D deficient diet treated with paricalcitol(VDD+P). Serum biochemical were checked twice, at baseline and after the 22nd day of study. Results: There was no significant difference in baseline laboratory data between groups. At the end of the study, 1,25(OH)D3 was reduced in VDD (P = 0.019) and VDD+P (P < 0.001) with a more significant decline in VDD+P group. Serum level of FGF23 was reduced in VDD+P group compared to the control group (P = 0.011) and VDD group (P = 0.021). serum PTH in VDD group was higher than the control and VDD+P group (P = 0.036 and P = 0.038, respectively). Conclusion: The present study showed that paricalcitol could reduce FGF23 in vitamin D deficient rats without any changes in serum calcium, phosphorous and fractional excretion of phosphorous, which might be due to low PTH and 1,25(OH)2 D3.

Solid dispersion-based spray-drying improves solubility and mitigates beany flavour of pea protein isolate

2019 ◽  
Vol 278 ◽  
pp. 665-673 ◽  
Author(s):  
Yang Lan ◽  
Minwei Xu ◽  
Jae-Bom Ohm ◽  
Bingcan Chen ◽  
Jiajia Rao
Keyword(s):  
Spray Drying ◽  
Solid Dispersion ◽  
Protein Isolate ◽  
Pea Protein ◽  
Beany Flavour ◽  
Pea Protein Isolate

scholarly journals Improvement of pea protein isolate powder properties by agglomeration in a fluidized bed: comparison between binder solutions

2018 ◽  
Author(s):  
Osvaldir Pereira Taranto ◽  
R. F. Nascimento ◽  
K Andreola ◽  
J. G. Rosa
Keyword(s):  
Fluidized Bed ◽  
Gum Arabic ◽  
Protein Isolate ◽  
Free Flow ◽  
Pea Protein ◽  
Agglomeration Process ◽  
Powder Properties ◽  
Wetting Time ◽  
Binder Liquid ◽  
Pea Protein Isolate

This study aimed to compare the agglomeration process of pea protein isolate (PPI) using water and aqueous gum Arabic solution as binder liquids. Drying air temperature and binder flow rate were set at 75 °C and 3.1 mL/min, respectively. Moisture content, mean particle size, wetting time and flowability were analyzed. Using water as binder liquid, the responses were (4.0 ± 0.4)%, 316.13 ± 16.73 μm, 10 s and free flow, respectively. Aqueous gum Arabic solution provided (2.9 ± 0.5)%, 462.67 ± 51.23 μm, 3 s and free flow as responses. Gum Arabic solution showed to be a more promising binder.Keywords: Agglomeration; Pulsed fluidized bed; Pea protein isolate; Wetting time; Flowability

scholarly journals Hypoglycemic Potential of Dietary Supplementation of Protein Isolate from Fermented Cucumeropsis manii in Streptozotocin Induced Hyperglycemic in Male Wistar Albino Rats

2021 ◽  
pp. 31-42
Author(s):  
A. O. Abiola ◽  
A. O. Iyoribhe ◽  
S. A. Adeniyi ◽  
O. B. Adu ◽  
A. S. Ogunbowale ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Protein Isolate ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Antioxidant Enzyme Activities ◽  
Cvd Risk ◽  
Streptozotocin Induced Diabetes

The effect of Protein isolate from fermented melon seeds (Ogiri Protei Isolates; OPI) of Cucumeropsis manii on blood glucose, lipid profile, and antioxidant enzyme activities in streptozotocin (STZ)-induced diabetic rats was investigated. Thirty Male Wistar rats were divided into five equal groups. GThe first control group with no exposure. The second group of rats with Streptozotocin-induced non-treated diabetes. The 3rd and 4th groups of rats with Streptozotocin-induced diabetes supplemented with Ogiri protein isolates (200, 600 mg/kg in diet). And the 5th group of rats with Streptozotocin-induced diabetes administered glibenclamide in a dose 500 ug/kg in diet [17]. The OPI was administered for 6 weeks. The administration of OPI reduced the blood glucose concentration of the STZ-induced diabetic rats. Sera and hepatic superoxide dismutase, activities of the STZ-induced diabetic rats were significantly (P< 0.05) increased in comparison with the diabetic control rats. Lipid peroxidation of the supplemented OPI diabetic rats was significantly (P< 0.05) decreased in comparison with the diabetic control rats as the administration of OPI to the STZ-induced diabetic rats significantly increased the enzymes’ activities. The concentration of low-density lipoproteins in the OPI supplemented rats was significantly elevated. These data demonstrate that OPI supplements might be beneficial for correcting hyperglycemia but the consumption of OPI can modulate some tissue lipids in a direction not beneficial for CVD risk in patients with diabetes.

scholarly journals 25-hydroxyvitamin D effect on cancer colon: Is visceral obesity the link?

2021 ◽  
Author(s):  
Mohamed M. Aboelnaga ◽  
Engy Mossad Aboelnaga ◽  
Abdullah Nahed ◽  
Mostafa El Shaer
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Visceral Obesity ◽  
Control Group ◽  
Visceral Adiposity Index ◽  
Group 13 ◽  
Hydroxyvitamin D ◽  
Inflammatory Status ◽  
Obesity And Cancer ◽  
Cancer Côlon

Abstract Background: low 25-hydroxyvitamin have been identified in pathogenesis of colorectal cancer (CRC) with survival affection. Visceral fat tissue predisposes to chronic inflammation and release of growth factors that mediate colonic neoplasia.Aim of the work: to evaluate effect of vitamin D status and visceral obesity on cancer colon and to report the possible link between vitamin D and visceral obesity in those patients. Patients & Methods: Our patients were distributed in two groups; group 1 included cancer colon cases (no= 60) and 2nd group included control cases (no= 40). Clinical, anthropometric and pathological data were collected. Calculation of body mass index, visceral adiposity index (VAI) and detection of Vitamin D (25 OHD) serum level were performed to compare between groups. Results: There were significant differences in VAI and level of 25 OHD between both groups(P=0.011). We found significant higher prevalence of vitamin d deficiency in patient’ group [32 (53.3%) versus control group 13 (32.5%)]. There was a significant lower mean of VAI in vitamin D deficient patients versus non deficient cases (P=0.024). A significant different means of VAI and Vitamin D in patients’ group with different TNM stages. As higher stages associated with lower level of Vitamin D (P=0.027) and higher VAI (P=0.031)Conclusion: The vitamin D may augment the inflammatory status in visceral obesity which reported to be involved in tumourgenesis of cancer colon. Vitamin D deficiency may be a mediator between obesity and cancer and associated with higher tumour stage.

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