Investigating the effect of paricalcitol on serum FGF23 in vitamin D deficient rats

Abstract Background: We conducted this study to discover changes of serum FGF23 in non-uremic rat model of vitamin D deficiency without secondary hyperparathyroidism using paricalcitol. Methods: 30 adult male rats weighting 300±20 grams were enrolled. They were divided into three groups of 10 rats including Control, Vitamin D deficient(VDD), and Vitamin D deficient diet treated with paricalcitol(VDD+P). Serum biochemical were checked twice, at baseline and after the 22nd day of study. Results: There was no significant difference in baseline laboratory data between groups. At the end of the study, 1,25(OH)D3 was reduced in VDD (P = 0.019) and VDD+P (P < 0.001) with a more significant decline in VDD+P group. Serum level of FGF23 was reduced in VDD+P group compared to the control group (P = 0.011) and VDD group (P = 0.021). serum PTH in VDD group was higher than the control and VDD+P group (P = 0.036 and P = 0.038, respectively). Conclusion: The present study showed that paricalcitol could reduce FGF23 in vitamin D deficient rats without any changes in serum calcium, phosphorous and fractional excretion of phosphorous, which might be due to low PTH and 1,25(OH)2 D3.

Download Full-text

PARATHYROID HORMONE AND BIOCHEMICAL METABOLIC BONE MARKERS IN PATIENTS WITH β-THALASSEMIA MAJOR IN MISAN PROVINCE / IRAQ

Periódico Tchê Química ◽

10.52571/ptq.v17.n35.2020.06_al-zuhairy_pgs_65_72.pdf ◽

2020 ◽

Vol 17 (35) ◽

pp. 65-72

Author(s):

Noor AL-Huda Salah AL-ZUHAIRY ◽

Zainab Abdul Jabbar Ridha AL-ALI

Keyword(s):

Vitamin D ◽

Alkaline Phosphatase ◽

Thalassemia Major ◽

Control Group ◽

Female Patients ◽

Metabolic Bone ◽

Significant Difference ◽

Calcium Phosphorus ◽

Male Patients ◽

Serum Pth

Beta-thalassemia is a heterogeneous group of hereditary blood disorders characterized by defects in the synthesis of the β- chains of hemoglobin, resulting in variable phenotypes ranging from severe anemia to clinically asymptomatic individuals. This study aims to assess the serum PTH, vitamin D, calcium, phosphorus, alkaline phosphatase, and magnesium levels in β-thalassemia major patients. A total of 50 (30 male and 20 female) patients with β- thalassemia major with ages range 11- 16 years and an equal number of sex-matched healthy adolescents as a control group were included in this study. A total of 52% of patients were lived in an urban area, and there was no significant difference between patients and the control group regarding residency. Male patients showed low statistically significant (P 0.05) mean serum PTH, vitamin D, and calcium levels, but mean serum phosphorus and alkaline phosphatase levels were significantly higher (P 0.05) as compared to the male control group. However, female patients had low, but without statistical significant (P>0.05) mean serum PTH level, whereas vitamin D and calcium levels were highly significant (P 0.05) reduced. The phosphorus and ALP levels were highly significantly (P 0.05) increased as compared to female controls. Regarding β- thalassemia major group, the current study showed male patients had non-significant (P 0.05) higher levels of PTH, calcium, phosphorus, and ALP. In contrast, vitamin D level was non-significantly (P 0.05) low in male patients as compared to female patients. Mean serum level of PTH had a negative correlation with phosphorus, but it had a positive association with vitamin D, calcium, ALP, and magnesium. In conclusion, this study demonstrated that β-thalassemia major patients have a markedly deranged biochemical metabolic bone profile. Regular monitoring of PTH and biochemical mineral profile is also recommended.

Download Full-text

Pengaruh Pemberian Minuman Energi Terhadap Kadar Serum Kreatinin Rattus Norvegicus

Jurnal Ilmiah Kedokteran Wijaya Kusuma ◽

10.30742/jikw.v10i1.1045 ◽

2021 ◽

Vol 10 (1) ◽

pp. 92

Author(s):

Budi Santo ◽

Dorta Simamora

Keyword(s):

Serum Creatinine ◽

Kidney Function ◽

Rattus Norvegicus ◽

Energy Drinks ◽

Energy Drink ◽

Male Rats ◽

Control Group ◽

Study Results ◽

Significant Difference ◽

One Way Anova

The energy drink is a beverage that is increasingly being used by the community with the aim to improve work performance, vitality, and concentration. The energy drink is also suspected to affect kidney function. This study aims to determine whether there is influence of various brands of energy drinks (M1, M2, M3, M4) of the serum creatinine levels in male rats Rattus norvegicus.This is a study with randomized post test only control group design. The study used male rats Rattus norvegicus as many as 25 animals that were divided into 5 groups. The control group, the group P1 = M1 energy drinks, group P2 = M2 energy drinks, group P3 = M3 energy drinks, group P4 = M4 energy drinks. The energy drinks were given as many as 2.5 ml / 200 g weight / day for 1 month. Cardiac blood sampling performed to measure the levels of serum creatinine and then the results were analyzed using One-Way Anova to compare the levels of serum creatinine in each group of this study. Results of One-Way Anova test obtained by sig α = 0.000 which means there is significant difference between the treatment group and the control group. It can be concluded that the administration of energy drinks to the Rattus norvegicus would be altered the kidney function. It is caused by various substances contained in energy drinks such as taurine, sugar, vitamin B, caffeine, and other supplementary materials.

Download Full-text

Evaluation of nanoselenium (Nano-Se) effect on hematological and serum biochemical parameters of rat in experimentally lead poisoning

Human & Experimental Toxicology ◽

10.1177/0960327116651124 ◽

2016 ◽

Vol 36 (4) ◽

pp. 421-427 ◽

Cited By ~ 3

Author(s):

A Jafari Dehkordi ◽

AN Mohebbi ◽

MR Aslani ◽

SM Ghoreyshi

Keyword(s):

Biochemical Parameters ◽

Thiobarbituric Acid ◽

Male Rats ◽

Control Group ◽

Tbars Level ◽

Serum Biochemical Parameters ◽

Selenium Compounds ◽

Significant Difference ◽

Serum Biochemical ◽

Hematological And Biochemical Parameters

The present study was designed to evaluate the effect of nanoselenium (Nano-Se) on hematological and biochemical parameters of rats experimentally intoxicated with lead (Pb). Thirty male rats were randomly divided into six groups as follows: the control, selenite, Nano-Se, Pb group, Pb + selenite, and Pb + Nano-Se groups. After 35 days, blood was collected from rats and hematology and serum biochemical parameters of oxidative stress were measured. The thiobarbituric acid reactive substances (TBARS) level of Pb group was significantly higher than other groups. Also, TBARS level was significantly lower in the Pb + Nano-Se group than Pb + selenite group. The serum superoxide dismutase activities were significantly lower in Pb group than the control, Pb + selenite, and Pb + Nano-Se groups. The catalase activities in the Pb group showed no significant change when compared to other groups. In the Pb group, packed cell volume was lower than the control group. A significant difference was observed between the control group and the Pb, Pb + selenite, and Pb + Nano-Se groups. In the Pb group, the numbers of white blood cell (WBC) decreased in comparison with the control group. Also, there was significant increase in WBC counts in the Pb + Nano-Se and Pb + selenite groups in comparison with Pb group. The number of lymphocytes in the Pb group decreased in comparison with the control group. By comparing the means of the Pb + Nano-Se and Pb + selenite groups together, it was determined that there were significant differences in the lymphocytes and neutrophil counts. In conclusion, usage of selenium compounds particularly Nano-Se particles inhibits the adverse effects of Pb on antioxidant activity and immune system function in the Pb poisoning.

Download Full-text

A Novel Rat Model of Vitamin D Deficiency: Safe and Rapid Induction of Vitamin D and Calcitriol Deficiency without Hyperparathyroidism

BioMed Research International ◽

10.1155/2015/604275 ◽

2015 ◽

Vol 2015 ◽

pp. 1-5 ◽

Cited By ~ 12

Author(s):

Andrea W. D. Stavenuiter ◽

Maria Vittoria Arcidiacono ◽

Evelina Ferrantelli ◽

Eelco D. Keuning ◽

Marc Vila Cuenca ◽

...

Keyword(s):

Vitamin D ◽

Vitamin D Deficiency ◽

Hypovitaminosis D ◽

Vitamin D Metabolites ◽

Deficient Diet ◽

Hydroxyvitamin D ◽

Dihydroxyvitamin D ◽

Study Results ◽

Wide Range ◽

Serum Pth

Vitamin D deficiency is associated with a range of clinical disorders. To study the mechanisms involved and improve treatments, animal models are tremendously useful. Current vitamin D deficient rat models have important practical limitations, including time requirements when using, exclusively, a vitamin D deficient diet. More importantly, induction of hypovitaminosis D causes significant fluctuations in parathyroid hormone (PTH) and mineral levels, complicating the interpretation of study results. To overcome these shortcomings, we report the successful induction of vitamin D deficiency within three weeks, with stable serum PTH and minerals levels, in Wistar rats. We incorporated two additional manoeuvres compared to a conventional diet. Firstly, the vitamin D depleted diet is calcium (Ca) enriched, to attenuate the development of secondary hyperparathyroidism. Secondly, six intraperitoneal injections of paricalcitol during the first two weeks are given to induce the rapid degradation of circulating vitamin D metabolites. After three weeks, serum 25-hydroxyvitamin D3(25D) and 1,25-dihydroxyvitamin D3(1,25D) levels had dropped below detection limits, with unchanged serum PTH, Ca, and phosphate (P) levels. Therefore, this model provides a useful tool to examine the sole effect of hypovitaminosis D, in a wide range of research settings, without confounding changes in PTH, Ca, and P.

Download Full-text

Stabilization of Vitamin D in Pea Protein Isolate Nanoemulsions Increases Its Bioefficacy in Rats

Nutrients ◽

10.3390/nu11010075 ◽

2019 ◽

Vol 11 (1) ◽

pp. 75 ◽

Cited By ~ 7

Author(s):

Ali M. Almajwal ◽

Mahmoud M. A. Abulmeaty ◽

Hao Feng ◽

Nawaf W. Alruwaili ◽

Astrid Dominguez-Uscanga ◽

...

Keyword(s):

Vitamin D ◽

Protein Isolate ◽

Normal Diet ◽

Control Group ◽

Albino Rats ◽

Deficient Diet ◽

Pea Protein ◽

Hydroxyvitamin D ◽

Serum Pth ◽

Pea Protein Isolate

Micronutrient delivery formulations based on nanoemulsions can enhance the absorption of nutrients and bioactives, and thus, are of great potential for food fortification and supplementation strategies. The aim was to evaluate the bioefficacy of vitamin D (VitD) encapsulated in nanoemulsions developed by sonication and pH-shifting of pea protein isolate (PPI) in restoring VitD status in VitD-deficient rats. Weaned male albino rats (n = 35) were fed either normal diet AIN-93G (VitD 1000 IU/kg) (control group; n = 7) or a VitD-deficient diet (<50 IU/kg) for six weeks (VitD-deficient group; n = 28). VitD-deficient rats were divided into four subgroups (n = 7/group). Nano-VitD and Oil-VitD groups received a dose of VitD (81 µg) dispersed in either PPI-nanoemulsions or in canola oil, respectively, every other day for one week. Their control groups, Nano-control and Oil-control, received the respective delivery vehicles without VitD. Serum 25-hydroxyvitamin D [25(OH)VitD], parathyroid hormone (PTH), Ca, P, and alkaline phosphatase (ALP) activity were measured. After one week of treatment, the VitD-deficient rats consuming Nano-VitD recovered from Vitamin D deficiency (VDD) as compared against baseline and had serum 25(OH)VitD higher than the Nano-control. Enhancement in VitD status was followed with expected changes in serum PTH, Ca, P, and ALP levels, as compared against the controls. Stabilization of VitD within PPI-based nanoemulsions enhances its absorption and restores its status and biomarkers of bone resorption in VitD-deficient rats.

Download Full-text

The Effect of Single and Triple Testicular Biopsy Using Biopty Gun on Spermatogenesis in Pubertal Rats

Animals ◽

10.3390/ani11061569 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1569

Author(s):

Tomislav Šušnjar ◽

Ivana Kuzmić Prusac ◽

Ivan Švagelj ◽

Anđela Jurišić ◽

Tomislav Šušnjar ◽

...

Keyword(s):

Sperm Count ◽

Semen Analysis ◽

Study Groups ◽

Testicular Biopsy ◽

Male Rats ◽

Lower Percentage ◽

Control Group ◽

Sprague Dawley ◽

Left Testis ◽

Significant Difference

Background: The aim of this study was to compare consequences in single and triple testicular biopsy by biopty gun in pubertal rats using histological and immunohistochemical analysis. Methods: Thirty-two Sprague-Dawley male rats were used as the experimental model. The rats were randomly divided into three study groups. The rats from the first group (n = 12) received a single-biopsy of upper pole of the left testis, while the rats from the second group (n = 10) received triple-biopsy of upper and lower poles and lateral surface of left testis. The third group (n = 10) was a control group. On the eightieth day after the biopsy in all rats bilateral orchiectomy and funiculectomy were performed to obtain testicular tissue and sperm for analysis. The consequences of the puncture were observed by pathohistology, immunohistochemistry and semen analysis. Results: The results of the study showed lower percentage of sperm count (14.5 mill/mL vs. 16 mill/mL, p = 0.130), sperm motility (24.6% vs. 32.7%, p > 0.05), abnormal sperm (30% vs. 27%, p > 0.05), atrophic tubules (21% vs. 6%, p < 0.001), volume (1.7 mL vs. 2.28 mL, p < 0.01) and apoptotic index (1.56 vs. 1.19, p = 0.650) in the testes with a triple-biopsy compared to the testes with a single-biopsy. Semen analysis showed a borderline significant difference between the group with triple-biopsy where sperm count was lower than it in the control group (14.5 mill/mL vs. 17.5 mill/mL, p = 0.05). A single-biopsy has little effect on the testis, especially on overall fertility. A triple-biopsy showed higher degree of the testicular damage but without a significant impact on overall fertility. Semen analysis showed that single- and triple-biopsies did not have a significant effect on sperm count, motility and morphology. Conclusion: Biopty gun procedure is a cheap, simple and reliable method for testicular biopsy in rats without a significant effect on sperm count, motility and morphology.

Download Full-text

Role of vitamin D serum levels in prevention of primary and recurrent melanoma

Scientific Reports ◽

10.1038/s41598-021-85294-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

M. Lombardo ◽

A. Vigezzi ◽

G. Ietto ◽

C. Franchi ◽

V. Iori ◽

...

Keyword(s):

Vitamin D ◽

Serum Levels ◽

Mitotic Rate ◽

Control Group ◽

Healthy Population ◽

Histological Subtype ◽

Melanoma Diagnosis ◽

Significant Difference ◽

Tumour Location

AbstractPatients afflicted with melanoma show lower vitamin D serum levels (VDSL) than the healthy population. This hypothesis agrees with its well-known antiproliferative features. An observational study was carried out to collect VDSL in patients suffering from melanoma. Our aim was to identify a potential connection between low VDSL and the risk to incur melanoma. Furthermore, we studied the association between VDSL at the diagnosis of melanoma and other germane prognostic factors. The population held in regard was composed of 154 patients with a diagnosis of melanoma between 2016 and 2019. These patients were retrospectively collected from our follow-up storage. We compared VDSL to clinical and pathological parameters (age, sex, tumour location, Breslow’s depth, Clark’s level, histological subtype, ulceration, et aliqua). Moreover, we recruited a control group with negative melanoma history. Mean and median of VDSL were significantly lower in the melanoma group. Instead, we found a negative association between melanoma and VDSL > 30 ng/L (OR 0.11; p < 0.0001). No correlation between VDSL and both Breslow’s depth and Clark’s level was discovered, but the VDSL comparison between thin (depth ≤ 1 mm) and thick tumours (depth > 1 mm) revealed a statistically significant difference (21.1 ± 8.2 ng/L vs 17.8 ± 8.1; p = 0.01). Moreover, VDSL were significantly lower in melanomas with mitotic rate ≥ 1/mm2 (22.1 ± 8.3 ng/L; p < 0007). Nevertheless, no connection was found between VDSL and both ulceration and positive sentinel nodes (p = 0.76; p = 0.74). Besides, our study revealed no association between VDSL and histological subtype (p = 0.161). Lower VDSL correlate with thick and high mitotic rate tumours. Future prospective studies would investigate if appropriate upkeep of suitable VDSL can decrease the risk of primary and recurrent melanoma diagnosis.

Download Full-text

The Combined Effects of Sports Smart Bracelet and Multi-Component Exercise Program on Exercise Motivation among the Elderly in Macau

Medicina ◽

10.3390/medicina57010034 ◽

2021 ◽

Vol 57 (1) ◽

pp. 34

Author(s):

Cheuk Kei Lao ◽

Bing Long Wang ◽

Richard S. Wang ◽

Hsiao Yun Chang

Keyword(s):

Artificial Intelligence ◽

Exercise Training ◽

Training Programs ◽

Exercise Program ◽

The Elderly ◽

Control Group ◽

Exercise Motivation ◽

Sport Training ◽

Study Results ◽

Significant Difference

Background and objectives: Faced with the serious problem of an aging population, exercise is one of the most effective ways to maintain the health of the elderly. In recent years, with the popularization of smartphones, the elderly have increasingly accepted technological products that incorporate artificial intelligence (AI). However, there is not much research on using artificial intelligence bracelets to enhance elders’ motivation and participation in exercise. Therefore, the purpose of this study is to evaluate the effectiveness of the combination of sports smart bracelets and multi-sport training programs on the motivation of the elderly in Macau. Materials and Methods: The study was conducted with a randomized trial design in a 12 week multi-sport exercise training intervention. According to the evaluation, a total of sixty elders’ pre- and post-test data were included in this study. Results: After 12 weeks of multi-sport exercise training, the evaluation scores on the exercise motivation scale (EMS) increased significantly in the group wearing exercise bracelets and those taking part in the multi-component exercise program, and the degree of progress reached a statistically significant level, but the control group did not show any statistically significant difference. The influence of the combination of sports smart bracelets and multi-sport training programs on elders’ motivation is clearer. Conclusions: The use of sports smart bracelets by elderly people in conjunction with diverse exercise training can effectively enhance elders’ motivation and increase their participation in regular exercise. The combination of sports smart bracelets and multi-sport training programs is worth promoting in the elderly population.

Download Full-text

673 Effect of Subcutaneous Topical Ozone Therapy on Second Degree Burn Wounds in Rats: An Experimental Study

Journal of Burn Care & Research ◽

10.1093/jbcr/irab032.319 ◽

2021 ◽

Vol 42 (Supplement_1) ◽

pp. S193-S193

Author(s):

Emre Karakaya ◽

Aydincan Akdur ◽

H Ebru ◽

Ayvazoglu Soy ◽

Alev Ok Atilgan ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Silver Sulfadiazine ◽

Male Rats ◽

Control Group ◽

Ozone Therapy ◽

Burn Wounds ◽

Degree Burn ◽

Significant Difference ◽

Second Degree

Abstract Introduction Burn is one of the most severe traumas that causes coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burn may affect the patient’s survival and reduce the complications that may be seen. In the present study we aimed effects of subcutaneous ozone injection on second degree burn wound. Methods A total of 72 Sprague-Dawley male rats included in the study were divided randomly into three groups (control group (CG), silver sulfadiazine group (SG), ozone group (OG)) and each group was divided randomly two subgroups (as sacrificed on d7 and on d14).A deep second degree scald burns were created on the lower back. In CG subcutaneous 0.9% serum saline was injected daily into the burn area. In SG, burns were dressed with silver sulfadiazine daily and in OG subcutaneous ozone was injected daily into the burn area. Tissue hydroxyproline level measurement and histopathological evaluation were done. Results When the groups were compared in terms of weight change, no significant difference was found on the 7th and 14th days. In the evaluation made in terms of tissue hydroxyproline, tissue hydroxyproline level in OG was found to be significantly higher on both the 7th and 14th days (p < 0.001). In histopathological evaluations, it was determined that wound healing in OG was significantly higher than in the other groups. Conclusions According to the results, subcutaneous ozone therapy is more effective than silver sulphadiazine in the healing process of second-degree burn wounds and it can be safely used in the treatment of burn wounds.

Download Full-text

Evaluation of the Relationship between Vitamin D Deficiency and Atherogenic Factors in Diabetic Patients with Metabolic Syndrome

Tabari Biomedical Student Research Journal ◽

10.18502/tbsrj.v3i4.7794 ◽

2021 ◽

Author(s):

Leila Akbarbaglu ◽

Elham Nozari Mirarkolaei ◽

Massoumeh Hotelchi ◽

Abbas Khonakdar-Tarsi ◽

Mahboobeh Ghanbari ◽

...

Keyword(s):

Metabolic Syndrome ◽

Vitamin D ◽

Serum Level ◽

Total Cholesterol ◽

Vitamin D3 ◽

Atherogenic Index ◽

Control Group ◽

Diabetic Patients ◽

Significant Difference ◽

25 Oh Vitamin D

Introduction: Metabolic syndrome includes a range of disorders that increase the risk of cardiovascular disease and diabetes mellitus. In this study, we examined the serum level of vitamin D3 in diabetic individuals with metabolic syndrome compared with non-diabetic individuals without metabolic syndrome and the association of serum vitamin D3 levels with metabolic syndrome and atherogenic factor (LDL/HDL). Material and Methods: In a case-control study, we included 110 women with metabolic syndrome according to ATP III criteria and 127 healthy women as a control group. Serum concentration of total cholesterol, LDL-C, FBS, HDL-C and serum triglyceride determined by enzymatic method and colorimetric and, serum level 25-(OH) vitamin D determined by ELISA. Results: It was found that the two healthy and metabolic groups were significantly different in terms of total cholesterol levels, LDL and triglyceride levels, HDL, VLDL, FBS, atherogenic index (LDL/HDL) and vitamin D levels (p<0.05). All participants in the control group and the patient and the whole study population were divided into two categories of insufficient and sufficient based on their measured serum concentrations of 25-(OH) vitamin D. There was a significant difference between the group with insufficient levels of vitamin D in comparison with the group with sufficient levels of vitamin D in terms of total cholesterol, LDL and triglyceride levels, HDL, VLDL, FBS and atherogenic index (LDL/HDL) (p=0.000). Conclusion: The present results showed that there is a significant relationship between level 25-(OH) D and atherogenic index (LDL/HDL) and the incidence of metabolic syndrome.

Download Full-text