scholarly journals First Identification and Genotyping of Enterocytozoon bieneusi and Prevalence of Encephalitozoon intestinalis in Patients with Acute Diarrhea in the Republic of Korea

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1424
Author(s):  
Ji-Young Kwon ◽  
Ji-Ye Seo ◽  
Tae-Yun Kim ◽  
Hee-Il Lee ◽  
Jung-Won Ju
Keyword(s):  
Acute Diarrhea ◽  
Pcr Amplification ◽  
Small Subunit ◽  
Enterocytozoon Bieneusi ◽  
Republic Of Korea ◽  
Internal Transcribed Spacer Region ◽  
Severe Diarrhea ◽  
Encephalitozoon Intestinalis ◽  
The Republic ◽  
Group 1

Encephalitozoon intestinalis and Enterocytozoon bieneusi can cause diarrhea in humans, especially severe diarrhea in immunocompromised patients. However, there have been few studies on Enc. intestinalis and Ent. bieneusi in patients with acute diarrhea in the Republic of Korea (ROK). In this study, fecal samples were collected from 1241 patients with acute diarrhea in 2020. Among these, 24 cases of Enc. intestinalis and one case of Ent. bieneusi were detected via PCR amplification of small subunit ribosomal RNA. Genotyping of the internal transcribed spacer region sequence revealed that the detected Ent. bieneusi genotype was in Group 1. This study provides the first evidence that Ent. bieneusi exists in humans in addition to animals in the ROK. To identify the causative agent, continuous monitoring of Enc. intestinalis and Ent. bieneusi is necessary for patients with acute diarrhea in the ROK.

Marasmioid and gymnopoid fungi of the Republic of Korea. 8. Gymnopus section Impudicae

Phytotaxa ◽  
2016 ◽  
Vol 286 (2) ◽  
pp. 75 ◽  
Author(s):  
RHIM RYOO ◽  
VLADIMÍR ANTONÍN ◽  
KANG-HYEON KA ◽  
MICHAL TOMŠOVSKÝ
Keyword(s):  
Dna Sequences ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Republic Of Korea ◽  
Rrna Gene ◽  
Internal Transcribed Spacer Region ◽  
Drying Up ◽  
The Republic ◽  
First Time ◽  
Ribosomal Rrna

Collections of Gymnopus sect. Impudicae from the Republic of Korea are evaluated. Three recorded species (G. densilamellatus, G. similis, and G. variicolor) are described as new for the science: Gymnopus densilamellatus is macroscopically characterized as having a brown to reddish brown, sometimes pale ochraceous and later whitish pileus and crowded lamellae. Gymnopus similis has an ochraceous-grey, brownish orange or brownish red pileus, and moderately distant, whitish, later dirty yellowish, greyish, or brownish orange or light brown lamellae. Gymnopus variicolor is characterized by a reddish brown, brownish red or violet brown pileus drying-up to light brown or brown, greyish brown or greyish red, later pale brownish orange lamellae, and greyish brown or greyish red pileus, grey-brown, light to reddish brown in upper part. Gymnopus dysodes is recorded for the first time in Korea. The morphological characteristics of the species are provided with a key for their identification. The taxonomic positions of all taxa are confirmed by the DNA sequences of the internal transcribed spacer region and the large subunit of ribosomal rRNA gene.

scholarly journals Molecular Detection and Genotyping of Intestinal Microsporid-ia from Stray Dogs in Iran

2019 ◽  
Author(s):  
Moein DELROBAEI ◽  
Shahram JAMSHIDI ◽  
Parviz SHAYAN ◽  
Elahe EBRAHIMZADE ◽  
Iraj ASHRAFI TAMAI ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Small Subunit ◽  
Enterocytozoon Bieneusi ◽  
Capital City ◽  
Urine Samples ◽  
Internal Transcribed Spacer Region ◽  
Stray Dogs ◽  
Transmission Potential ◽  
Time Period ◽  
Specific Fragment

Background: Microsporidia as one of the most important pathogens in veterinary and agricultural settings, have emerged in immunocompromised patients in Iran. To date, different Enterocytozoon bieneusi genotypes have been identified in humans and animals, supporting the possibility of zoonotic zoonosis transmission potential. The aim of this study was to evaluate the distribution of E. bieneusi genotypes among overpopulated stray dogs in vicinity of Tehran, the capital city of Iran. Methods: Totally, 75 stool and 75 urine samples were obtained from 75 stray dogs during the time period from Mar 2015 to Oct 2015. DNA extraction was performed on all the samples and specific fragment of small subunit ribosomal RNA gene of E. bieneusi and Encephalitozoon spp. was amplified. Furthermore, specific primers targeting the internal transcribed spacer region of E. bieneusi were applied to determine the genotype of the microorganism. Results: Microsporidia was detected in 5.3% of stool samples, while none of the urine samples was positive for microsporidia species. Overall, 440 bp fragment of E. bieneusi was amplified in all the samples and there was no amplification for Encephalitozoon spp. The results of sequencing of 410 bp fragment of internal transcribed spacer region showed that all the E. bieneusi were genotype D. Conclusion: E. bieneusi was the most prevalent microsporidian species in the stray dogs and all the positive isolates were characterized as genotype D.

scholarly journals Identification of Encephalitozoon intestinalis in Travelers with Chronic Diarrhea by Specific PCR Amplification

1998 ◽  
Vol 36 (1) ◽  
pp. 37-40 ◽  
Author(s):  
Laurent Raynaud ◽  
Frederic Delbac ◽  
Veronique Broussolle ◽  
Meja Rabodonirina ◽  
Veronique Girault ◽  
...  
Keyword(s):  
Chronic Diarrhea ◽  
Pcr Amplification ◽  
Small Subunit ◽  
Small Subunit Rrna ◽  
Specific Pcr ◽  
Encephalitozoon Intestinalis ◽  
Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Screening ◽  
Virus Screening

With the use of Weber’s modified trichrome and Uvitex 2B techniques, spores of microsporidia were detected in the stools of four travelers presenting clinically with chronic diarrhea. The general health of these patients was not impaired, and human immunodeficiency virus screening was negative. Immune evaluation, including the study of lymphocytic subpopulations, assay of serum immunoglobulins, and an intradermal multitest, showed normal results. Molecular identification of microsporidian species was based on the PCR amplification of a small-subunit rRNA sequence followed by HinfI endonuclease restriction. Encephalitozoon intestinalis microsporidiosis was thus shown in two of the four patients examined. In two patients, therapy based on albendazole made stools devoid of microsporidian spores without influence on the intestinal disorders. The pathogenic role of E. intestinalis in immunocompetent individuals remains to be demonstrated.

scholarly journals First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China

Parasite ◽  
2019 ◽  
Vol 26 ◽  
pp. 24 ◽  
Author(s):  
Run Luo ◽  
Leiqiong Xiang ◽  
Haifeng Liu ◽  
Zhijun Zhong ◽  
Li Liu ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Its Region ◽  
Enterocytozoon Bieneusi ◽  
Southwestern China ◽  
Zoonotic Potential ◽  
Age Group ◽  
Tibetan Pig ◽  
Tibetan Pigs ◽  
Intestinal Pathogen ◽  
Group 1

Enterocytozoon bieneusi is a common intestinal pathogen in a variety of animals. While E. bieneusi genotypes have become better-known, there are few reports on its prevalence in the Tibetan pig. This study investigated the prevalence, genetic diversity, and zoonotic potential of E. bieneusi in the Tibetan pig in southwestern China. Tibetan pig feces (266 samples) were collected from three sites in the southwest of China. Feces were subjected to PCR amplification of the internal transcribed spacer (ITS) region. Enterocytozoon bieneusi was detected in 83 (31.2%) of Tibetan pigs from the three different sites, with 25.4% in Kangding, 56% in Yaan, and 26.7% in Qionglai. Prevalence varies according to age group, from 24.4% (age 0–1 years) to 44.4% (age 1–2 years). Four genotypes of E. bieneusi were identified: two known genotypes EbpC (n = 58), Henan-IV (n = 24) and two novel genotypes, SCT01 and SCT02 (one of each). We compare our results with a compilation of published results on the host range and geographical distribution of E. bieneusi genotypes in China. Phylogenetic analysis showed these four genotypes clustered to group 1 with zoonotic potential. Multilocus sequence typing (MLST) analysis of three microsatellites (MS1, MS3, MS7) and one minisatellite (MS4) was successful in 47, 48, 23 and 47 positive specimens and identified 10, 10, 5 and 5 genotypes at four loci, respectively. This study indicates the potential danger of E. bieneusi to Tibetan pigs in southwestern China, and offers basic advice for preventing and controlling infections.

scholarly journals Confirmation of the Human-Pathogenic Microsporidia Enterocytozoon bieneusi, Encephalitozoon intestinalis, and Vittaforma corneae in Water

1998 ◽  
Vol 64 (9) ◽  
pp. 3332-3335 ◽  
Author(s):  
Scot E. Dowd ◽  
Charles P. Gerba ◽  
Ian L. Pepper
Keyword(s):  
Surface Water ◽  
Gastrointestinal Disease ◽  
Pcr Amplification ◽  
Enterocytozoon Bieneusi ◽  
Species Level ◽  
Screening Methods ◽  
Specific Pcr ◽  
Encephalitozoon Intestinalis ◽  
Wide Range ◽  
Homology Comparison

ABSTRACT Microsporidia, as a group, cause a wide range of infections, though two species of microsporidia in particular,Enterocytozoon bieneusi and Encephalitozoon intestinalis, are associated with gastrointestinal disease in humans. To date, the mode of transmission and environmental occurrence of microsporidia have not been elucidated due to lack of sensitive and specific screening methods. The present study was undertaken with recently developed methods to screen several significant water sources. Water concentrates were subjected to community DNA extraction followed by microsporidium-specific PCR amplification, PCR sequencing, and database homology comparison. A total of 14 water concentrates were screened; 7 of these contained human-pathogenic microsporidia. The presence of Encephalitozoon intestinalis was confirmed in tertiary sewage effluent, surface water, and groundwater; the presence of Enterocytozoon bieneusi was confirmed in surface water; and the presence ofVittaforma corneae was confirmed in tertiary effluent. Thus, this study represents the first confirmation, to the species level, of human-pathogenic microsporidia in water, indicating that these human-pathogenic microsporidia may be waterborne pathogens.

scholarly journals Occurrence and Potentially Zoonotic Genotypes of Enterocytozoon Bieneusi in Wild Rhesus Macaques (Macaca Mulatta) Living in Nanwan Monkey Island, Hainan, China: A Public Health Concern

2021 ◽  
Author(s):  
Wei Zhao ◽  
Huan-Huan Zhou ◽  
Guang-Xu Ren ◽  
Yu Qiang ◽  
Hui-Cong Huang ◽  
...  
Keyword(s):  
Public Health ◽  
Macaca Mulatta ◽  
Rhesus Macaques ◽  
Enterocytozoon Bieneusi ◽  
Health Concern ◽  
Pcr Analysis ◽  
Rrna Gene ◽  
Internal Transcribed Spacer Region ◽  
Novel Genotype ◽  
Group 1

Abstract Background Enterocytozoon bieneusi, a microsporidian species, is a zoonotic pathogen found in both humans and animals. Here, we determined the prevalence, explored the different genotypes of E. bieneusi in wild rhesus macaques (Macaca mulatta) (Hainan Island of China), and assessed their zoonotic potential. Methods We collected 173 fecal specimens from wild M. mulatta living in Nanwan Monkey Island, Hainan, China. Subsequently, we identified and genotyped E. bieneusi using nested PCR analysis amplification of the internal transcribed spacer region (ITS) of the rRNA gene. Lastly, a neighbor-joining tree was built based on gene sequences from the ITS region of E. bieneusi. Results Of the 173 specimens from wild M. mulatta, 26 (15%) were infected with E. bieneusi. We identified six genotypes of E. bieneusi, of which five were known: PigEBITS7 (n = 20), D (n = 2), Type IV (n = 1), Peru6 (n = 1), Henan-III (n = 1), and a novel genotype: HNM-IX (n = 1). From the phylogenetic analysis, the six genotypes identified here were all categorized into zoonotic group 1. Conclusion Based on the results taht the novel genotype falling under zoonotic group 1 and all the known genotypes found in humans, we conclude that the wild M. mulatta infected with E. bieneusi have a public health significance.

scholarly journals First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China

2018 ◽  
Author(s):  
Run Luo ◽  
Leiqiong Xiang ◽  
Haifeng Liu ◽  
Zhijun Zhong ◽  
Li Liu ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Its Region ◽  
Enterocytozoon Bieneusi ◽  
Southwestern China ◽  
Zoonotic Potential ◽  
Tibetan Pig ◽  
Enteric Diseases ◽  
Tibetan Pigs ◽  
Intestinal Pathogen ◽  
Group 1

AbstractEnterocytozoon bieneusi is a common intestinal pathogen and a major cause of diarrhea and enteric diseases in a variety of animals. While the E. bieneusi genotype has become better-known, there are few reports on its prevalence in the Tibetan pig. This study investigated the prevalence, genetic diversity, and zoonotic potential of E. bieneusi in the Tibetan pig in southwestern China. Tibetan pig feces (266 samples) were collected from three sites in the southwest of China. Feces were subjected to PCR amplification of the internal transcribed spacer (ITS) region. E. bieneusi was detected in 83 (31.2%) of Tibetan pigs from the three different sites, with 25.4% in Kangding, 56% in Yaan and 26.7% in Qionglai. Age group demonstrated the prevalence of E. bieneusi range from 24.4%(aged 0 to 1 years) to 44.4%(aged 1 to 2 years). Four genotypes of E. bieneusi were identified: two known genotypes EbpC (n=58), Henan-IV (n=24) and two novel genotypes, SCT01 and SCT02 (one of each). Phylogenetic analysis showed these four genotypes clustered to group 1 with zoonotic potential. Multilocus sequence typing (MLST) analysis three microsatellites (MS1, MS3, MS7) and one minisatellite (MS4) revealed 47, 48, 23 and 47 positive specimens were successfully sequenced, and identified ten, ten, five and five genotypes at four loci, respectively. This study indicates the potential danger of E. bieneusi to Tibetan pigs in southwestern China, and offers basic data for preventing and controlling infections.

scholarly journals Molecular Detection and Genotyping of Enterocytozoon bieneusi in Black Goats (Capra hircus) in Yunnan Province, Southwestern China

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3387
Author(s):  
Shi-Chen Xie ◽  
Yang Zou ◽  
Zhao Li ◽  
Jian-Fa Yang ◽  
Xing-Quan Zhu ◽  
...  
Keyword(s):  
Yunnan Province ◽  
Control Strategies ◽  
Pcr Amplification ◽  
Enterocytozoon Bieneusi ◽  
Southwestern China ◽  
Capra Hircus ◽  
Effective Control ◽  
Group 2 ◽  
And Gender ◽  
Group 1

Enterocytozoon bieneusi is a fungus-like protist that can parasitize in the intestines of humans and various animals causing a threat to public health. However, there has been no data for E. bieneusi prevalence and genotypes in black goats in Yunnan Province, Southwestern China. In this study, 907 fecal samples were collected from black goats in 5 counties from Yunnan Province. The prevalence and genotypes of E. bieneusi were examined by nested PCR amplification targeting the nuclear internal transcribed spacer (ITS). Multi-locus sequence typing (MLST) was used to further examine the potential occurrence of genetic segregation. The overall prevalence of E. bieneusi in black goats in Yunnan Province was 10.3% (93/907). Statistical analysis revealed that E. bieneusi prevalence was significantly associated with the region, age and gender of black goats (p < 0.001). Four new genotypes (CYG-1, CYG-2, CYG-3, CYG-4) and 11 known genotypes (CHG1, CHG2, CHG3, CHG5, CHG28, J, D, BEB6, Wildboar3, CD6, SDD1) of E. bieneusi were identified. At the microsatellite and minisatellite loci, 15, 2, 17, and 33 sequences were obtained, respectively, forming one new multi-locus genotype (MLG27). Phylogenetic analysis revealed that all 15 genotypes were clustered into group 1 and group 2, with zoonotic potential. This is the first report of E. bieneusi prevalence and genotypes in black goats in Yunnan Province, China. Effective control strategies and measures should be taken to reduce the risk of E. bieneusi transmission between black goats, other animals, and humans.

FIRST REPORT OF ENTEROCYTOZOON BIENEUSI FROM AN AFRICAN LION (PANTHERA LEO) IN A ZOO IN THE REPUBLIC OF KOREA

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Sunwoo Hwang ◽  
Jeongho Kim ◽  
Yu-Jin Park ◽  
Dong-Hun Jang ◽  
Seung-Uk Shin ◽  
...  
Keyword(s):  
Enterocytozoon Bieneusi ◽  
Republic Of Korea ◽  
Panthera Leo ◽  
First Report ◽  
African Lion ◽  
The Republic

scholarly journals Confirmed detection of Cyclospora cayetanesis, Encephalitozoon intestinalis and Cryptosporidium parvum in water used for drinking

2003 ◽  
Vol 1 (3) ◽  
pp. 117-123 ◽  
Author(s):  
Scot E. Dowd ◽  
David John ◽  
James Eliopolus ◽  
Charles P. Gerba ◽  
Jaime Naranjo ◽  
...  
Keyword(s):  
Cryptosporidium Parvum ◽  
Rural Areas ◽  
Gastrointestinal Disease ◽  
Pcr Amplification ◽  
Enterocytozoon Bieneusi ◽  
Human Consumption ◽  
Screening Methods ◽  
Sequence Information ◽  
Encephalitozoon Intestinalis ◽  
Public Consumption

Human enteropathogenic microsporidia (HEM), Cryptosporidium parvum, Cyclospora cayetanesis, and Giardia lamblia are associated with gastrointestinal disease in humans. To date, the mode of transmission and environmental occurrence of HEM (Encephalitozoon intestinalis and Enterocytozoon bieneusi) and Cyclospora cayetanesis have not been fully elucidated due to lack of sensitive and specific environmental screening methods. The present study was undertaken with recently developed methods, to screen various water sources used for public consumption in rural areas around the city of Guatemala. Water concentrates collected in these areas were subjected to community DNA extraction followed by PCR amplification, PCR sequencing and computer database homology comparison (CDHC). All water samples screened in this study had been previously confirmed positive for Giardia spp. by immunofluorescent assay (IFA). Of the 12 water concentrates screened, 6 showed amplification of microsporidial SSU-rDNA and were subsequently confirmed to be Encephalitozoon intestinalis. Five of the samples allowed for amplification of Cyclospora 18S-rDNA; three of these were confirmed to be Cyclospora cayetanesis while two could not be identified because of inadequate sequence information. Thus, this study represents the first confirmed identification of Cyclospora cayetanesis and Encephalitozoon intestinalis in source water used for consumption. The fact that the waters tested may be used for human consumption indicates that these emerging protozoa may be transmitted by ingestion of contaminated water.

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