scholarly journals Clostridium perfringens-Induced Host-Pathogen Transcriptional Changes in the Small Intestine of Broiler Chickens

Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1607
Author(s):  
Mingmin Lu ◽  
Baohong Yuan ◽  
Xianghe Yan ◽  
Zhifeng Sun ◽  
Hyun S. Lillehoj ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Broiler Chickens ◽  
Molecular Mechanisms ◽  
Statistical Significance ◽  
Transcriptional Analysis ◽  
P Value ◽  
Bacterial Disease ◽  
Necrotic Enteritis ◽  
Mrna Levels ◽  
Host Pathogen

Clostridium perfringens is an important opportunistic pathogen that may result in toxin-mediated diseases involving food poisoning/tissue gangrene in humans and various enterotoxaemia in animal species. It is a main etiological agent for necrotic enteritis (NE), the most financially devastating bacterial disease in broiler chickens, especially if raised under antibiotic-free conditions. Importantly, NE is responsible for losses of six billion US dollars annually in the global poultry industry. To investigate the molecular mechanisms of C. perfringens-induced pathogenesis in the gut and its microbiome mRNA levels in C. perfringens-infected and non-infected hosts, we used RNA sequencing technology to perform transcriptional analysis of both host intestine and microbiome using our NE model. The growth rate was significantly impaired in chickens infected by C. perfringens. In total, 13,473 annotated chicken genes were differentially expressed between these two groups, with ninety-six genes showing statistical significance (|absolute fold changes| > 2.0, adjusted p value < 0.05). Genes involved in energy production, MHC Class I antigen, translation, ribosomal structures, and amino acid, nucleotide and carbohydrate metabolism from infected gut tissues were significantly down-regulated. The upregulated genes were mainly engaged in innate and adaptive immunity, cellular processes, genetic information processing, and organismal systems. Additionally, the transcriptional levels of four crucial foodborne pathogens were significantly elevated in a synergic relationship with pathogenic C. perfringens infection. This study presents the profiling data that would likely be a relevant reference for NE pathogenesis and may provide new insights into the mechanism of host-pathogen interaction in C. perfringens-induced NE infection in broiler chickens.

scholarly journals Supplementing Garlic Nanohydrogel Optimized Growth, Gastrointestinal Integrity and Economics and Ameliorated Necrotic Enteritis in Broiler Chickens Using a Clostridium perfringens Challenge Model

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2027
Author(s):  
Doaa Ibrahim ◽  
Tamer Ahmed Ismail ◽  
Eman Khalifa ◽  
Shaimaa A. Abd El-Kader ◽  
Dalia Ibrahim Mohamed ◽  
...  
Keyword(s):  
Growth Performance ◽  
Clostridium Perfringens ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Economic Benefits ◽  
Production Costs ◽  
Broiler Chicks ◽  
Necrotic Enteritis ◽  
Feed Conversion ◽  
Intestinal Lesion

Necrotic enteritis (NE) caused by Clostridium perfringens (C. perfringens) results in impaired bird growth performance and increased production costs. Nanotechnology application in the poultry industry to control NE outbreaks is still not completely clarified. Therefore, the efficacy of dietary garlic nano-hydrogel (G-NHG) on broilers growth performance, intestinal integrity, economic returns and its potency to alleviate C. perfringens levels using NE challenge model were addressed. A total of 1200 male broiler chicks (Ross 308) were assigned into six groups; four supplemented with 100, 200, 300 or 400 mg of G-NHG/kg diet and co-challenged with C. perfringens at 21, 22 and 23 d of age and two control groups fed basal diet with or without C. perfringens challenge. Over the total growing period, the 400 mg/kg G-NHG group had the most improved body weight gain and feed conversion efficiency regardless of challenge. Parallel with these results, the mRNA expression of genes encoding digestive enzymes (alpha 2A amylase (AMY2A), pancreatic lipase (PNLIP) and cholecystokinin (CCK)) and intestinal barriers (junctional adhesion molecule-2 (JAM-2), occludin and mucin-2 (Muc-2)) were increased in groups fed G-NHG at higher levels to be nearly similar to those in the unchallenged group. At 14 d post challenge, real-time PCR results revealed that inclusion of G-NHG led to a dose-dependently decrease in the C. perfringens population, thereby decreasing the birds’ intestinal lesion score and mortality rates. Using 400 mg/kg of G-NHG remarkably ameliorated the adverse effects of NE caused by C. perfringens challenge, which contributed to better growth performance of challenged birds with rational economic benefits.

The Effects of Berberine on Clostridium Perfringens Induced Necrotic Enteritis in Broiler Chickens

2017 ◽  
Vol 08 (03) ◽  
Author(s):  
David Xiang Y ◽  
Zhiyong H ◽  
Wenyue W ◽  
Colin P ◽  
Zhi cheng X
Keyword(s):  
Clostridium Perfringens ◽  
Broiler Chickens ◽  
Necrotic Enteritis

scholarly journals Draft Genome Sequence of Clostridium perfringens Strain TAMU, Which Causes Necrotic Enteritis in Broiler Chickens

2020 ◽  
Vol 9 (4) ◽  
Author(s):  
Catherine Ausland ◽  
Adil Sabr Al-Ogaili ◽  
Juan D. Latorre ◽  
Guillermo Tellez-Isaias ◽  
Billy M. Hargis ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Genome Sequence ◽  
Broiler Chickens ◽  
Draft Genome ◽  
Gastrointestinal Diseases ◽  
Intervention Strategies ◽  
Draft Genome Sequence ◽  
Necrotic Enteritis ◽  
Content Type ◽  
Deadly Disease

Clostridium perfringens causes severe gastrointestinal diseases, which include necrotic enteritis (NE) in chickens, a deadly disease worldwide. We report here the draft genome sequence of Clostridium perfringens strain TAMU, which was used in developing an NE chicken challenge model. This C. perfringens TAMU genome sequence will aid in advancing potential intervention strategies to reduce NE pathogenesis.

scholarly journals Quantification of Cell Proliferation and Alpha-Toxin Gene Expression of Clostridium perfringens in the Development of Necrotic Enteritis in Broiler Chickens

2007 ◽  
Vol 73 (21) ◽  
pp. 7110-7113 ◽  
Author(s):  
Weiduo Si ◽  
Joshua Gong ◽  
Yanming Han ◽  
Hai Yu ◽  
John Brennan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Regression Model ◽  
Clostridium Perfringens ◽  
Broiler Chickens ◽  
Toxin Gene ◽  
Necrotic Enteritis ◽  
Lesion Score ◽  
Alpha Toxin ◽  
Toxin Gene Expression

ABSTRACT Cell proliferation and alpha-toxin gene expression of Clostridium perfringens in relation to the development of necrotic enteritis (NE) were investigated. Unlike bacitracin-treated chickens, non-bacitracin-treated birds exhibited typical NE symptoms and reduced growth performance. They also demonstrated increased C. perfringens proliferation and alpha-toxin gene expression that were positively correlated and progressed according to the regression model y = b 0 + b 1 X − b 2 X 2. The average C. perfringens count of 5 log10 CFU/g in the ileal digesta appears to be a threshold for developing NE with a lesion score of 2.

scholarly journals Bacillus subtilis DSM 32315 Supplementation Attenuates the Effects of Clostridium perfringens Challenge on the Growth Performance and Intestinal Microbiota of Broiler Chickens

2019 ◽  
Vol 7 (3) ◽  
pp. 71 ◽  
Author(s):  
Cristiano Bortoluzzi ◽  
Bruno Serpa Vieira ◽  
Juliano Cesar de Paula Dorigam ◽  
Anita Menconi ◽  
Adebayo Sokale ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Clostridium Perfringens ◽  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Dietary Supplementation ◽  
Necrotic Enteritis ◽  
Feed Conversion ◽  
Negative Effects ◽  
Infected Control

The objective of this study was to evaluate the effects of the dietary supplementation of Bacillus subtilis DSM 32315 (probiotic) on the performance and intestinal microbiota of broiler chickens infected with Clostridium perfringens (CP). One-day-old broiler chickens were assigned to 3 treatments with 8 replicate pens (50 birds/pen). The treatments were: non-infected control; infected control; and infected supplemented with probiotic (1 × 106 CFU/g of feed). On day of hatch, all birds were sprayed with a coccidia vaccine based on the manufacturer recommended dosage. On d 18–20 the infected birds were inoculated with CP via feed. Necrotic enteritis (NE) lesion score was performed on d 21. Digestive tract of 2 birds/pen was collected on d 21 to analyze the ileal and cecal microbiota by 16S rRNA sequencing. Performance was evaluated on d 28 and 42. On d 21, probiotic supplementation reduced (p < 0.001) the severity of NE related lesion versus infected control birds. On d 28, feed efficiency was improved (p < 0.001) in birds supplemented with probiotic versus infected control birds. On d 42, body weight gain (BW gain) and feed conversion ratio (FCR) were improved (p < 0.001) in probiotic supplemented birds versus infected control birds. The diversity, composition and predictive function of the intestinal microbial digesta changed with the infection but the supplementation of probiotic reduced these variations. Therefore, dietary supplementation of Bacillus subtilis DSM 32315 was beneficial in attenuating the negative effects of CP challenge on the performance and intestinal microbiota of broilers chickens.

Bacteriophage Therapy for Control of Necrotic Enteritis of Broiler Chickens Experimentally Infected With Clostridium perfringens

2010 ◽  
Vol 5 (1) ◽  
pp. e28-e29 ◽  
Author(s):  
Ross W. Miller ◽  
James Skinner ◽  
Alexander Sulakvelidze ◽  
Greg F. Mathis ◽  
Charles L. Hofacre
Keyword(s):  
Clostridium Perfringens ◽  
Broiler Chickens ◽  
Necrotic Enteritis ◽  
Bacteriophage Therapy

scholarly journals The Mycotoxin Deoxynivalenol Predisposes for the Development of Clostridium perfringens-Induced Necrotic Enteritis in Broiler Chickens

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e108775 ◽  
Author(s):  
Gunther Antonissen ◽  
Filip Van Immerseel ◽  
Frank Pasmans ◽  
Richard Ducatelle ◽  
Freddy Haesebrouck ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Broiler Chickens ◽  
Necrotic Enteritis

scholarly journals Immunization of Broiler Chickens against Clostridium perfringens-Induced Necrotic Enteritis

2007 ◽  
Vol 14 (9) ◽  
pp. 1070-1077 ◽  
Author(s):  
R. R. Kulkarni ◽  
V. R. Parreira ◽  
S. Sharif ◽  
J. F. Prescott
Keyword(s):  
Clostridium Perfringens ◽  
Broiler Chickens ◽  
Hypothetical Protein ◽  
Oral Infection ◽  
Secreted Proteins ◽  
Enzyme Linked Immunosorbent Assay ◽  
Necrotic Enteritis ◽  
Alpha Toxin ◽  
Genes Encoding ◽  
Antibody Titers

ABSTRACT Necrotic enteritis (NE) in broiler chickens is caused by Clostridium perfringens. Currently, no vaccine against NE is available and immunity to NE is not well characterized. Our previous studies showed that immunity to NE followed oral infection by virulent rather than avirulent C. perfringens strains and identified immunogenic secreted proteins apparently uniquely produced by virulent C. perfringens isolates. These proteins were alpha-toxin, glyceraldehyde-3-phosphate dehydrogenase, pyruvate:ferredoxin oxidoreductase (PFOR), fructose 1,6-biphosphate aldolase, and a hypothetical protein (HP). The current study investigated the role of each of these proteins in conferring protection to broiler chickens against oral infection challenges of different severities with virulent C. perfringens. The genes encoding these proteins were cloned and purified as histidine-tagged recombinant proteins from Escherichia coli and were used to immunize broiler chickens intramuscularly. Serum and intestinal antibody responses were assessed by enzyme-linked immunosorbent assay. All proteins significantly protected broiler chickens against a relatively mild challenge. In addition, immunization with alpha-toxin, HP, and PFOR also offered significant protection against a more severe challenge. When the birds were primed with alpha-toxoid and boosted with active toxin, birds immunized with alpha-toxin were provided with the greatest protection against a severe challenge. The serum and intestinal washings from protected birds had high antigen-specific antibody titers. Thus, we conclude that there are certain secreted proteins, in addition to alpha-toxin, that are involved in immunity to NE in broiler chickens.

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