scholarly journals Genotyping and Antimicrobial Susceptibility of Clostridium perfringens and Clostridioides difficile in Camel Minced Meat

Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1640
Author(s):  
Mahmoud Fayez ◽  
Waleed R. El-Ghareeb ◽  
Ahmed Elmoslemany ◽  
Saleem J. Alsunaini ◽  
Mohamed Alkafafy ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Clostridium Perfringens ◽  
Antimicrobial Susceptibility ◽  
Type A ◽  
High Rate ◽  
Rrna Gene ◽  
Clostridioides Difficile ◽  
Meat Samples ◽  
Minced Meat ◽  
Butcher Shops

The present study aimed to determine the occurrence, genotypes, and antimicrobial resistance of Clostridium perfringens (C. perfringens) and Clostridioides difficile (C. difficile) in camel minced meat samples collected from small butcher shops and supermarkets in Al-Ahsa Governorate, Saudi Arabia. A total of 100 camel minced meat samples were randomly collected from small butcher’s shops (n = 50) and supermarkets (n = 50) in Al-Ahsa Governorate, Saudi Arabia. C. perfringens and C. difficile were isolated and identified using the VITEK-2 compact system and 16S rRNA gene amplification. Genotypes, toxin genes, and antimicrobial susceptibility of the isolates were determined. Moreover, ELISA was used to detect C. perfringens and C. difficile toxins. C. perfringens and C. difficile were isolated from 14% and 4% of the tested minced meat samples, respectively. Out of the 14 C. perfringens isolates, type A (64.3%), type B (7.1%), type C (21.5%), and type D (7.1%) were detected. However, out of the four C. difficile isolates, three (75%) were type A+B+ and one (25%) was type A−B+. None of the C. perfringens or C. difficile toxins were identified using ELISA. C. perfringens and C. difficile isolates exhibited a high rate of resistance to tetracycline (56% and 75%, respectively). However, all isolates were susceptible to amoxicillin-clavulanate. Multidrug resistance was observed in three (21.4%) C. perfringens and one (25%) C. difficile isolates. In conclusion, camel minced meat was contaminated with C. perfringens and C. difficile, which present a potential risk of food poisoning. The majority of the isolates were resistant to at least one antimicrobial, and some isolates were multidrug-resistant. Therefore, food safety standards and frequent inspections of abattoirs, small butcher shops, and supermarkets should be enforced.

scholarly journals Prevalence of resistant Staphylococcus aureus strains in frozen meat and their control using phytosynthesized selenium nanoparticles

2021 ◽  
Vol 48 (4) ◽  
Author(s):  
Mohammed S. Al-Saggaf ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Saudi Arabia ◽  
Molecular Identification ◽  
Resistance Genes ◽  
Meat Products ◽  
Multidrug Resistant ◽  
Selenium Nanoparticles ◽  
Phyllanthus Emblica ◽  
Meat Samples ◽  
Minced Meat

Staphylococcus aureus continually threatens the safety of meat products, especially the multidrug-resistant (MDR) strains. The screening of MDR S. aureus prevalence in marketed meat products in Saudi Arabia was conducted via molecular identification of resistance genes (cfr, gyrA, and gyrB) that occurred in isolated bacteria. The green phytosynthesis of selenium nanoparticles (Se-NPs) was also conducted, using the fruits’ extract of Phyllanthus Emblica (PeE), for their evaluation as antibacterial nanocomposites against MDR S. aureus isolates. The prevalence percentage (PP) of S. aureus in meat samples was 14.2%, where the MDR S. aureus prevalence was 9.2%. The highest prevalence of S. aureus isolates was attained from minced meat samples, whereas the highest PP for multidrug-resistant (MDR) S. aureus was recorded from sausages samples. The PeE-synthesized Se-NPs had negative charges, spherical shapes, and well-disperssion with mean diameters of 11.98 nm. The anti- S. aureus activities of PeE, phytosynthesized Se-NPs, and their composite (PeE/Se-NPs) were proved qualitatively and quantitatively against the different standard and MDR strains, the antibacterial action of PeE/Se-NPs was the strongest. The treatment of MDR S. aureus with PeE/Se-NPs led to severe cells’ lyses/explosion after 8 h of exposure. The nanocomposites from PeE/Se-NPs are recommended for controlling MDR S. aureus in meat.

scholarly journals Assessment of Microbiological Safety and Quality of Minced Meat and Meat Contact Surfaces in Selected Butcher Shops of Addis Ababa, Ethiopia

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Kibrom Zerabruk ◽  
Negussie Retta ◽  
Diriba Muleta ◽  
Anteneh T. Tefera
Keyword(s):  
Contact Surface ◽  
Addis Ababa ◽  
Cold Chain ◽  
Food Handlers ◽  
Microbial Counts ◽  
Surface Materials ◽  
The Mean ◽  
Meat Samples ◽  
Minced Meat ◽  
Butcher Shops

Food-borne pathogens are one of the leading causes of illness and death particularly in developing countries. This study was aimed at analyzing the hygiene indicator microorganisms and pathogens of minced meat and contact surface materials in butcher shops in Addis Ababa, Ethiopia. Additionally, a checklist was applied to evaluate the hygiene condition of the establishments, and a questionnaire/checklist was used to assess food safety knowledge of the food handlers. This study has indicated that the mean microbial counts (total aerobic mesophilic, staphylococci, Enterobacteriaceae, total coliforms, fecal coliforms, aerobic spores and yeasts/molds) of the minced meat and contact surface materials in butcher shops ranged between 2.35 and 6.50 log·cfu/g and between 1.80 and 6.30 log·cfu/cm2, respectively. The mean microbial counts of minced meat samples taken in the morning and afternoon showed statistically significant differences (p<0.05). The prevalence of E. coli, Salmonella, and S. aureus in minced meat and contact surface samples was exhibited as 43.75 and 29.17%, 6.25 and 4.17%, and 37.50 and 37.50% in that order. The study has indicated that minced meat samples and contact surface materials had higher microbial load with poor personal and work area sanitation. Low knowledge of food handlers in the butcher shops and broken cold chain have also been found as major contributing factors for the contamination of beef.

scholarly journals Retail chicken meats as potential sources of Clostridioides difficile in Al-Jouf, Saudi Arabia

2021 ◽  
Vol 15 (07) ◽  
pp. 972-978
Author(s):  
Ahmed Elsayed Taha Attia
Keyword(s):  
Saudi Arabia ◽  
Wide Spectrum ◽  
Human Infection ◽  
Chicken Meat ◽  
Isolation And Identification ◽  
Clostridioides Difficile ◽  
Potential Sources ◽  
Toxigenic Strains ◽  
Antibiotics Sensitivity ◽  
Meat Samples

Introduction: Presence of Clostridioides difficile in stool of food birds and animals is a risk for contamination of their meats to become potential sources of human infection. The main virulence factors of C. difficile are its resistance to antibiotics, production of toxins and spores. As far as I know, this is the first study to evaluate C. difficile prevalence in chicken meats, its toxigenic activities and antibiotics sensitivity patterns in Al-Jouf, Saudi Arabia. Methodology: Totally, 250 raw chicken meat samples were examined. Standard microbiological and biochemical procedures were used for C. difficile isolation and identification. The suspected colonies were tested by L-proline and C. difficile test kits then confirmed by Vitek 2 compact system. Xpect C. difficile toxin A/B test was used to detect A/B toxins production. Antibiotics susceptibility patterns were detected by Epsilon tests. Results: C. difficile was isolated from 11/250 (4.40%) chicken meat samples; 5/65 (7.69%) legs, 3/65 (4.61%) thighs, 2/60 (3.33%) wings and 1/60 (1.67%) breasts (p = 0.4). All isolates were non-toxigenic. Although all isolates were vancomycin sensitive, some isolates were intermediate/resistant to metronidazole, tetracycline, clindamycin or moxifloxacin antibiotics with variable degrees. Conclusions: C. difficile might contaminate retail chicken meats. Although low level of contamination by non-toxigenic strains was detected, chicken meats should be investigated as C. difficile infection sources for humans especially elders, immune-compromised and long terms wide spectrum antibiotics-used persons. Decreased sensitivity of C. difficile to antibiotics is emerging.

scholarly journals Clostridioides difficile in national food surveillance, Slovenia, 2015 to 2017

2020 ◽  
Vol 25 (16) ◽  
Author(s):  
Valerija Tkalec ◽  
Urska Jamnikar-Ciglenecki ◽  
Maja Rupnik ◽  
Stanka Vadnjal ◽  
Katja Zelenik ◽  
...  
Keyword(s):  
Plant Protection ◽  
Surveillance Programme ◽  
Clostridioides Difficile ◽  
Contaminated Food ◽  
Intestinal Pathogen ◽  
The Republic ◽  
Meat Samples ◽  
Minced Meat ◽  
Positive Results

Background Clostridioides difficile is an important human and animal intestinal pathogen. Because of increasing indications of an association between C. difficile and food, in 2015, the Administration of the Republic of Slovenia for Food Safety, Veterinary Sector and Plant Protection (UVHVVR) included C. difficile in its national food surveillance. Aim We aim to report the results and experience with a nationwide and long-term testing of food for C. difficile as a part of a regular national food surveillance programme. Methods Retail minced meat and meat preparations (beef, pork and poultry) were sampled within a three-year period, 2015 to 2017. Selected raw retail vegetables, leaf salads and root vegetables, and ready-to-eat salads were only sampled during 2016 and 2017. Seafood was only sampled in 2017. Results Altogether, 434 samples were tested, with 12 of 336 (3.6%) meat samples and 6 of 98 (6.1%) raw vegetables contaminated with C. difficile. Twelve of 18 recovered food isolates were toxigenic (toxinotypes 0, III, V, XII). The isolates belonged to 13 different PCR ribotypes, 001 being most common (5 isolates). Several food types with an increased potential of being contaminated with C. difficile were detected by surveillance. Conclusion The three-year C. difficile testing within the national food surveillance revealed a low proportion of C. difficile-contaminated food and high genotype variability. Because the risk of C. difficile infection associated with C. difficile-contaminated food is unknown, no measures were recommended in the case of positive results.

scholarly journals Genotyping and antimicrobial susceptibility of Clostridium perfringens isolated from Tinamidae, Cracidae and Ramphastidae species in Brazil

2014 ◽  
Vol 44 (3) ◽  
pp. 486-491 ◽  
Author(s):  
Rodrigo Otávio Silveira Silva ◽  
Francisco Carlos Ferreira Junior ◽  
Marcus Vinícius Romero Marques ◽  
Carlos Augusto Oliveira Junior ◽  
Nelson Rodrigo da Silva Martins ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Antimicrobial Susceptibility ◽  
Type A ◽  
Necrotic Enteritis ◽  
Toxin B ◽  
Stool Samples ◽  
Beta 2 ◽  
Encoding Gene

The aim of this study was to isolate, genotype and evaluate the antimicrobial susceptibility of Clostridium perfringens found in species Tinamidae, Cracidae and Ramphastidae in Brazil. C. perfringens was isolated in 13 (5%) out of 260 swab samples and five (8.3%) out of 60 stool samples. All strains were classified as C. perfringens type A, and nine (50%) were positive for the beta-2 toxin-encoding gene. No strains were positive for the necrotic enteritis toxin B-like (NetB)-encoding gene. All isolates were susceptible to penicillin, metronidazole and vancomycin, whereas four (22.2%), five (27.8%) and 13 (72.2%) strains were considered resistant to erythromycin, oxytetracycline and lincomycin, respectively.

scholarly journals Toxinotyping and antimicrobial resistance of Clostridium perfringens isolated from processed chicken meat products

2017 ◽  
Vol 61 (1) ◽  
pp. 53-58
Author(s):  
Dalia Hamza ◽  
Sohad Dorgham ◽  
Ashraf Hakim
Keyword(s):  
Clostridium Perfringens ◽  
Meat Products ◽  
Type A ◽  
Chicken Meat ◽  
Pcr Assay ◽  
Type D ◽  
Multiplex Pcr Assay ◽  
Meat Samples ◽  
Potential Public Health

Abstract Introduction: The toxinotyping and antimicrobial susceptibility of Clostridium perfringens strains isolated from processed chicken meat were determined. Material and Methods: Two hundred processed chicken meat samples from luncheon meats, nuggets, burgers, and sausages were screened for Clostridium perfringens by multiplex PCR assay for the presence of alpha (cpa), beta (cpb), epsilon (etx), iota (ia), and enterotoxin toxin (cpe) genes. The C. perfringens isolates were examined in vitro against eight antibiotics (streptomycin, amoxicillin, ampicillin, ciprofloxacin, lincomycin, cefotaxime, rifampicin, and trimethoprim-sulfamethoxazole) Results: An overall of 32 C. perfringens strains (16%) were isolated from 200 processed chicken meat samples tested. The prevalence of C. perfringens was significantly dependent on the type of toxin genes detected (P = 0.0), being the highest in sausages (32%), followed by luncheon meats (24%), burgers (6%), and nuggets (2%). C. perfringens type A was the most frequently present toxinotype (24/32; 75%), followed by type D (21.9 %) and type E (3.1%). Of the 32 C. perfringens strains tested, only 9 (28%) were enterotoxin gene carriers, with most representing type A (n = 6). C. perfringens strains differed in their resistance/susceptibility to commonly used antibiotics. Most of the strains tested were sensitive to ampicillin (97%) and amoxicillin (94%), with 100% of the strains being resistant to streptomycin and lincomycin. It is noteworthy that the nine isolates with enterotoxigenic potential had a higher resistance than the non-enterotoxigenic ones. Conclusion: The considerably high C. perfringens isolation rates from processed chicken meat samples and resistance to some of the commonly used antibiotics indicate a potential public health risk. Recent information about the isolation of enterotoxigenic C. perfringens type E from chicken sausage has been reported.

NGHIÊN CỨU SỰ NHẠY CẢM VỚI KHÁNG SINH CỦA MỘT SỐ CHỦNG VI KHUẨN GÂY VIÊM ĐƯỜNG HÔ HẤP CẤP Ở TRẺ EM DƯỚI 6 TUỔI TẠI BỆNH VIỆN NHI THANH HÓA 2009 - 2014

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Hoai Do Ngoc
Keyword(s):  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
High Rate ◽  
Isolation Rate

From 43.574 fluid nasopharynx speciments of  the chidren inpatient under six we isolated total 21.769 types bacteria with isolation rate : 49.95%. In which the highest isolation rate for H. influenza, S. pneumoniae and M. catarrhalis were 13,94%; 7,11%; 1,43% respectively. Antimicrobial susceptibility testing shown all the types of  for H. influenza, S. pneumoniae and M. catarrhalis good susses to Fosphomycine, S. pneumoniae and M. catarrhalis good susses to Imipenem, H. influenza good susses to Azithromycine, S. pneumoniae good susses to Penicilline and Piperacilline, M. catarrhalis good susses to Tobramycine and Ofloxacine. All of  H. influenza, S. pneumoniae and M. catarrhalis were reported resistance to Tri/Sulpha, Chloramphenicol, Erythromycine in high rate.

scholarly journals Temporal Variations in Patterns of Clostridioides difficile Strain Diversity and Antibiotic Resistance in Thailand

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 714
Author(s):  
Supapit Wongkuna ◽  
Tavan Janvilisri ◽  
Matthew Phanchana ◽  
Phurt Harnvoravongchai ◽  
Amornrat Aroonnual ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Susceptibility ◽  
Reference Strain ◽  
Toxin Production ◽  
Temporal Variations ◽  
Toxin Gene ◽  
Data Sets ◽  
Clostridioides Difficile ◽  
Life Threatening ◽  
Susceptibility Profiles

Clostridioides difficile has been recognized as a life-threatening pathogen that causes enteric diseases, including antibiotic-associated diarrhea and pseudomembranous colitis. The severity of C. difficile infection (CDI) correlates with toxin production and antibiotic resistance of C. difficile. In Thailand, the data addressing ribotypes, toxigenic, and antimicrobial susceptibility profiles of this pathogen are scarce and some of these data sets are limited. In this study, two groups of C. difficile isolates in Thailand, including 50 isolates collected from 2006 to 2009 (THA group) and 26 isolates collected from 2010 to 2012 (THB group), were compared for toxin genes and ribotyping profiles. The production of toxins A and B were determined on the basis of toxin gene profiles. In addition, minimum inhibitory concentration of eight antibiotics were examined for all 76 C. difficile isolates. The isolates of the THA group were categorized into 27 A−B+CDT− (54%) and 23 A-B-CDT- (46%), while the THB isolates were classified into five toxigenic profiles, including six A+B+CDT+ (23%), two A+B+CDT− (8%), five A−B+CDT+ (19%), seven A−B+CDT− (27%), and six A−B−CDT− (23%). By visually comparing them to the references, only five ribotypes were identified among THA isolates, while 15 ribotypes were identified within THB isolates. Ribotype 017 was the most common in both groups. Interestingly, 18 unknown ribotyping patterns were identified. Among eight tcdA-positive isolates, three isolates showed significantly greater levels of toxin A than the reference strain. The levels of toxin B in 3 of 47 tcdB-positive isolates were significantly higher than that of the reference strain. Based on the antimicrobial susceptibility test, metronidazole showed potent efficiency against most isolates in both groups. However, high MIC values of cefoxitin (MICs 256 μg/mL) and chloramphenicol (MICs ≥ 64 μg/mL) were observed with most of the isolates. The other five antibiotics exhibited diverse MIC values among two groups of isolates. This work provides evidence of temporal changes in both C. difficile strains and patterns of antimicrobial resistance in Thailand.

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