scholarly journals Study of Volatile Secondary Metabolites Present in Piper carpunya Leaves and in the Traditional Ecuadorian Beverage Guaviduca

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 338
Author(s):  
Eduardo Valarezo ◽  
Jonathan Xavier Rivera ◽  
Edgar Coronel ◽  
Miguel Andrés Barzallo ◽  
James Calva ◽  
...  
Keyword(s):  
Essential Oil ◽  
Sulfonic Acid ◽  
Folk Medicine ◽  
Liquid Extraction ◽  
Gram Negative Bacteria ◽  
Main Constituent ◽  
Strong Activity ◽  
Gram Negative ◽  
Enantiomeric Ratio ◽  
Liquid Liquid Extraction

Piper carpunya Ruiz & Pav. is an aromatic shrub native to Ecuador, the leaves of which are used to prepare the traditional beverage Guaviduca. Different health benefits are attributed to the guaviduca beverage, which is consumed as a traditional and folk medicine. In this study, fresh P. carpunya leaves were collected in the winter and summer and subjected to hydrodistillation for the extraction of the essential oil. The guaviduca beverage was prepared by infusion in water and the volatile compounds were isolated by liquid–liquid extraction. Chemical composition and enantioselective analyses were performed by gas chromatography. The antibacterial activity was assayed against Gram-positive and Gram-negative bacteria. The scavenging radical properties of the essential oil was evaluated by 2,2-diphenyl-1-picrylhydryl (DPPH) and 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. The acetylcholinesterase inhibitory activity was measured using the spectrophotometric method. The chemical analysis allowed us to identify more than 98% of the compounds in all samples. The main constituent of the essential oil was 1,8-cineole (25.20 ± 1.31%) in P. carpunya collected in winter and (17.45 ± 2.33%) in P. carpunya collected in summer, while in the beverage, there was 14 mg/L. Safrole was identified in the essential oil (PCW 21.91 ± 2.79%; PCS 13.18 ± 1.72%) as well as in the beverage (2.43 ± 0.12 mg/L). Enantioselective analysis was used to investigate the enantiomeric ratio and excess of four chiral components. The essential oil presented a strong activity against Klebsiella pneumoniae with a MIC of 500 μg/mL and a very strong anticholinesterase activity with an IC50 of 36.42 ± 1.15 µg/mL.

Coriander essential oil and linalool - interactions with antibiotics against Gram-positive and Gram-negative bacteria

2019 ◽  
Vol 68 (2) ◽  
pp. 156-164 ◽  
Author(s):  
P. Aelenei ◽  
C.M. Rimbu ◽  
E. Guguianu ◽  
G. Dimitriu ◽  
A.C. Aprotosoaie ◽  
...  
Keyword(s):  
Essential Oil ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative

scholarly journals In vitro antimicrobial and antioxidant activities of the essential oil of Craniotome furcata

2010 ◽  
Vol 2 (1) ◽  
pp. 57-62 ◽  
Author(s):  
R. K. Joshi
Keyword(s):  
Free Radical ◽  
Essential Oil ◽  
Radical Cation ◽  
Steam Distillation ◽  
Antioxidant Activities ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Aerial Parts ◽  
Tube Dilution

The aim of present study was, to investigate the essential oil of Craniotome furcata for its antimicrobial and antioxidant activities. The essential oil was obtained by steam distillation of the flowering aerial parts of C. furcata. The oil was tested for its antimicrobial activity in vitro against four Gram-positive, four Gram-negative bacteria and three fungi, by using disk diffusion and tube dilution methods. The antioxidant potential of the essential oil was investigated by two different models DPPH free radical and ABTS free radical cation scavenging. The essential oil was effective against all the tested microorganisms, with minimum inhibitory concentrations (MICs) ranged from 4.11±1.44 to 0.30±0.10 mg/ml. The oil showed antioxidant activity in DPPH free radical and ABTS free radical cation scavenging model. The results of this study revealed that the essential oil of C. furcata possesses antimicrobial and antioxidant activities.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Espeletia nana

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Alexis Peña ◽  
Luis Rojas ◽  
Rosa Aparicio ◽  
Libia Alarcón ◽  
José Gregorio Baptista ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).

scholarly journals Chemical Composition and in Vitro Antibacterial Activity of the Essential Oil of Minthostachys Mollis (Kunth) Griseb Vaught from the Venezuelan Andes

2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Flor D. Mora ◽  
María Araque ◽  
Luis B. Rojas ◽  
Rosslyn Ramírez ◽  
Bladimiro Silva ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Chemical Constituents ◽  
Salmonella Typhi ◽  
Inhibitory Effect ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Significant Inhibitory Effect ◽  
In Vitro Antibacterial Activity

Chemical constituents of the essential oil from the leaves of Minthostachys mollis (Kunth) Griseb Vaught var. mollis collected in January 2008 at Tuñame, Trujillo State, Venezuela, were separated and identified by GCMS analysis. The essential oil was obtained by hydrodistillation and thirteen components (98.5% of the sample) were identified by comparison with the Wiley GCMS library data base. The two major components were pulegone (55.2%) and trans-menthone (31.5%). The essential oil showed a significant inhibitory effect against Gram-positive and Gram-negative bacteria, especially Bacillus subtilis and Salmonella typhi (4 μg/mL).

Fractionation of citrus essential oil by liquid–liquid extraction using a perforated rotating disc contactor

2016 ◽  
Vol 163 ◽  
pp. 247-257 ◽  
Author(s):  
Daniel Gonçalves ◽  
Martin Emil Erismann Teschke ◽  
Cristina Chiyoda Koshima ◽  
Christianne Elisabete da Costa Rodrigues
Keyword(s):  
Essential Oil ◽  
Liquid Extraction ◽  
Rotating Disc ◽  
Liquid Liquid Extraction ◽  
Rotating Disc Contactor

Antibacterial Activity and Chemical Composition of the Essential Oil of Grammosciadium platycarpum Boiss. from Iran

2005 ◽  
Vol 60 (1-2) ◽  
pp. 30-34 ◽  
Author(s):  
Ali Sonboli ◽  
Fereshteh Eftekhar ◽  
Morteza Yousefzadi ◽  
Mohammad Reza Kanani
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Two Samples

The chemical composition of the essential oils obtained from two samples (GP1 and GP2) of Grammosciadium platycarpum Boiss. was analyzed by GC and GC-MS. The analysis of the oils resulted in the identification of twenty-two constituents. Linalool (79.0% - GP1, 81.8% - GP2) and limonene (10.0%, 5.8%) were found to be the major components, respectively. The in vitro antibacterial activities of these oils and their main compounds against seven Gram-positive and Gram-negative bacteria were investigated. The results exhibited that the total oils and their major components possess strong to moderate activities against all the tested bacteria except for Pseudomonas aeruginosa.

scholarly journals Antimicrobial Peptides from Rat-Tailed Maggots of the Drone Fly Eristalis tenax Show Potent Activity against Multidrug-Resistant Gram-Negative Bacteria

2020 ◽  
Vol 8 (5) ◽  
pp. 626 ◽  
Author(s):  
Rolf Hirsch ◽  
Jochen Wiesner ◽  
Armin Bauer ◽  
Alexander Marker ◽  
Heiko Vogel ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Multidrug Resistant ◽  
Therapeutic Window ◽  
Gram Negative Bacteria ◽  
Strong Activity ◽  
Gram Negative ◽  
Physiological Conditions ◽  
Manure Storage ◽  
Defense Molecules ◽  
Generation Sequencing

The spread of multidrug-resistant Gram-negative bacteria is an increasing threat to human health, because novel compound classes for the development of antibiotics have not been discovered for decades. Antimicrobial peptides (AMPs) may provide a much-needed breakthrough because these immunity-related defense molecules protect many eukaryotes against Gram-negative pathogens. Recent concepts in evolutionary immunology predict the presence of potent AMPs in insects that have adapted to survive in habitats with extreme microbial contamination. For example, the saprophagous and coprophagous maggots of the drone fly Eristalis tenax (Diptera) can flourish in polluted aquatic habitats, such as sewage tanks and farmyard liquid manure storage pits. We used next-generation sequencing to screen the E. tenax immunity-related transcriptome for AMPs that are synthesized in response to the injection of bacterial lipopolysaccharide. We identified 22 AMPs and selected nine for larger-scale synthesis to test their activity against a broad spectrum of pathogens, including multidrug-resistant Gram-negative bacteria. Two cecropin-like peptides (EtCec1-a and EtCec2-a) and a diptericin-like peptide (EtDip) displayed strong activity against the pathogens, even under simulated physiological conditions, and also achieved a good therapeutic window. Therefore, these AMPs could be used as leads for the development of novel antibiotics.

scholarly journals Determination of Chemical Composition and Antimicrobial Activity of Melaleuca cajuputi Essential Oil from Quang Tri Province, Vietnam

2020 ◽  
Vol 32 (9) ◽  
pp. 2203-2207
Author(s):  
TRAN QUOC TOAN ◽  
LAI PHUONG PHUONG THAO ◽  
NGUYEN QUYET CHIEN ◽  
NGUYEN THI HONG VAN ◽  
ÐOAN LAN PHUONG ◽  
...  
Keyword(s):  
Essential Oil ◽  
Maximum Yield ◽  
Batch Process ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Qualitative And Quantitative ◽  
Melaleuca Cajuputi ◽  
Main Components ◽  
Bactericidal Agent

The essential oil of Melaleuca cajuputi was obtained by hydrodistillation method. This work aims to adopt water as a solvent in a batch process to extract essential oil from Melaleuca cajuputi fresh leaves. The leaves are collected from Quang Tri Province, Vietnam. Analysis of constituents was performed by GC/MS. The maximum yield ranged from 0.6 to 0.7%. Several compounds have been identified in high quantities and meaningful qualitative and quantitative differences have been observed under different conditions. The main components of the M. cajuputi essential oil included eucalyptol (27.512%), γ-terpinene (8.59%), terpinolene (9.047%), β-eudesmene (3.359%), α- selinene (3.889%), α-terpineol (4.108%), 1R-α-pinene (2.158%), caryophyllene (6.48%) and α-caryophyllene (3.522%). This study has confirmed that the essential oil of M. cajuputi essential oil is a promising bactericidal agent on several Gram-positive and Gram-negative bacteria.

scholarly journals Essential Oil of Daucus glaber Forssk

2004 ◽  
Vol 59 (5-6) ◽  
pp. 373-378 ◽  
Author(s):  
El-Sayed S. Mansour ◽  
Galal T. Maatooq ◽  
Ashraf T. Khalil ◽  
El-Sayed M. Marwan ◽  
Amal A. Sallam
Keyword(s):  
Candida Albicans ◽  
Essential Oil ◽  
Volatile Oil ◽  
Antimicrobial Activities ◽  
Cytotoxic Activities ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Terpene Alcohols ◽  
Monoterpene Hydrocarbons

The composition of the essential oil of the fruits, leaves and stems of Daucus glaber Forssk has been studied by GC/MS. It was found that, the essential oil of the fruits consists of monoterpene hydrocarbons (limonene and sylvestrene are the majors) and phenylpropanoids (elemicin is the major). Sylvestrene has never been reported before in the essential oil of any Daucus species. The study of the essential oil of the leaves revealed the presence of monoterpene hydrocarbons; limonene and γ-terpinene are the majors and a small amount of sylvestrene. The essential oil of stems consists of monoterpene hydrocarbons (γ-terpinene is the major), terpene alcohols (mainly 4-terpineol) and phenylpropanoids (myristicin and elemicin are the majors). It is interesting that, the essential oil of the fruits is free from any oxygenated terpenes while that of the stems is free from limonene and sylvestrene which are present in the essential oil of the fruits and leaves in fairly large amounts. The essential oil of the fruits, leaves and stems shows broad antimicrobial activities against both gram positive and gram negative bacteria. In addition, the volatile oil of the stem, particularly, show activi­ties against Candida albicans (yeast). Also, the prepared oils have variable cytotoxic activities with LC50 21.52, 36.01 and 42.34 μrg/ml. respectively.

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