scholarly journals Detecting Genetic Mobility Using a Transposon-Based Marker System in Gamma-Ray Irradiated Soybean Mutants

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 373
Author(s):  
Nguyen Ngoc Hung ◽  
Dong-Gun Kim ◽  
Jae Il Lyu ◽  
Kyong-Cheul Park ◽  
Jung Min Kim ◽  
...  
Keyword(s):  
Inverted Repeat ◽  
Gamma Ray ◽  
Mutation Breeding ◽  
Terminal Inverted Repeat ◽  
Mutant Selection ◽  
Mutant Population ◽  
A Genome ◽  
Genetic Mobility ◽  
Gamma Irradiated ◽  
Mutant Lines

Transposable elements (TEs)—major components of eukaryotic genomes—have the ability to change location within a genome. Because of their mobility, TEs are important for genome diversification and evolution. Here, a simple rapid method, using the consensus terminal inverted repeat sequences of PONG, miniature inverted-repeat transposable element (MITE)-Tourist (M-t) and MITE-Stowaway (M-s) as target region amplification polymorphism (TE-TRAP) markers, was employed to investigate the mobility of TEs in a gamma-irradiated soybean mutant pool. Among the different TE-TRAP primer combinations, the average polymorphism level and polymorphism information content value were 57.98% and 0.14, respectively. Only the PONG sequence separated the mutant population into three major groups. The inter-mutant population variance, determined using the PONG marker (3.151 and 29%) was greater than that of the M-t (2.209 and 20%) and M-s (2.766 and 18%) markers, whereas the reverse was true for the intra-mutant population variations, with M-t and M-s values, being 15.151 (82%) and 8.895 (80%), respectively, compared with the PONG marker (7.646 and 71%). Thus, the MITE markers revealed more dynamic and active mobility levels than the PONG marker in gamma-ray irradiated soybean mutant lines. The TE-TRAP technique associated with sensitive MITEs is useful for investigating genetic diversity and TE mobilization, providing tools for mutant selection in soybean mutation breeding.

Impact of mutant varieties in Malaysia: challenges and future perspectives for mutation breeding.

2021 ◽  
pp. 65-75
Author(s):  
Rusli Ibrahim
Keyword(s):  
Gamma Ray ◽  
Nuclear Research ◽  
Mutation Breeding ◽  
Chrysanthemum Morifolium ◽  
High Yield ◽  
Research Centre ◽  
New Variety ◽  
Substantial Progress ◽  
Gamma Ray Irradiation ◽  
Gamma Irradiated

Abstract Malaysia has made substantial progress in plant mutation breeding with the use of nuclear techniques and related biotechnologies, not only in the development of new mutant varieties but also in the establishment of an excellent nuclear research centre. A total of 53 mutant varieties have been developed, including rice Oryza sativa (19), banana Musa acuminata (one), groundnut Arachis hypogaea (two), orchid Dendrobium 'Sonia' (six), chrysanthemum Chrysanthemum morifolium (seven), hibiscus Hibiscus rosa-sinensis (three), roselles Hibiscus sabdariffa L. (three) and other ornamental and landscaping plants (12). Most of the new ornamental varieties have been developed by both acute and chronic gamma-ray irradiation of seeds, rooted cuttings, bulbs and tissue cultures. Food crops that have an economic impact on sustainable agricultural production are mutant varieties of banana ('Novaria') and rice (MRQ74, MR219-9 and MR219-4). 'Novaria' is a selection made from a mutant, 'GN-60A', of 'Grande Naine' (AAA Musa) identified from gamma-ray treated populations of the Biotechnology Laboratory in Seibersdorf, Austria. 'Novaria' was the first mutant variety, officially released in 1995 by the Malaysian Nuclear Agency as a new variety for its improved characteristics such as early flowering, short stature and high yield. MRQ74 is a type of high-quality fragrant rice with newly induced traits such as resistance to blast, long and slender grain shape, non-sticky and with the elongation properties of cooked rice similar to those of Basmati-type rice. It is an indirect mutant variety released in 2003 and one of its parental lines for cross-breeding was the mutant 'Mahsuri', which was developed through mutation breeding using gamma-rays. In 2014, two new mutant varieties of rice, 'MR219-9' and 'MR219-4', which are drought tolerant, high yielding and resistant to blast, were selected from gamma irradiated material. Despite these achievements, applications of induced mutation have decreased during the past 10 years due to reduced funding. Mutation breeding is still a promising technique for the development of novel varieties which in combination with advanced molecular genetics can bring plant mutation breeding into a new era.

Gamma-ray induced pedigreed mutant population of tossa jute (Corchorus olitorius L.): a key resource for forward and reverse genetics.

2021 ◽  
pp. 469-477
Author(s):  
Shashi Bhushan Choudhary ◽  
Anjay Kumar Jambhulkar ◽  
Hariom Kumar Sharma ◽  
A. Anil Kumar ◽  
Neetu Kumari ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Reverse Genetics ◽  
Gamma Ray ◽  
Secondary Growth ◽  
Academic Progress ◽  
Mutation Breeding ◽  
Limiting Factor ◽  
Corchorus Olitorius ◽  
Mutant Population ◽  
Germplasm Diversity

Abstract Narrow genetic diversity in available germplasm is a serious limiting factor for academic progress and agronomic improvement of crops like Corchorus olitorius, an economically important bast fibre crop. Mutation breeding, with its proven ability to improve qualitative as well as quantitative traits, can be employed to augment germplasm diversity. In the present study, gamma-rays were used to treat the seeds of two promising varieties, JRO 204 and JRO 8432; LD50 doses for gamma-rays were 200 Gy and 300 Gy for JRO 204 and JRO 8432, respectively. Irradiation of two varieties has resulted in the development of a large number of macro-mutants, such as twisted bark, extreme dwarf, non-abscission leaf, soft stem, hard stem and round pod mutants. Morphological and anatomical studies of these mutants gave new light on secondary growth in the species. In addition to the academic utility, these mutants will prove of immense importance to plant breeders aiming to improve fibre quality. Moreover, novel mutants will help to develop new plant architecture suitable for diversified applications of the genus.

scholarly journals Evaluation of genetic variability between yellow vein mosaic disease resistant gamma-irradiated okra mutant and parent

2020 ◽  
pp. 38-42
Author(s):  
Sanoop Surendran ◽  
Punnakkal Sreedharan Udayan
Keyword(s):  
Genetic Variability ◽  
Gamma Ray ◽  
Mutation Breeding ◽  
Mosaic Disease ◽  
Yellow Vein ◽  
Fourth Generation ◽  
Disease Resistant ◽  
Polymorphic Bands ◽  
Gamma Irradiated ◽  
Yellow Vein Mosaic Disease

Okra (Abelmoschus esculentus L. Moench) is a member of the Malvaceae family. It has been cultivated as one of the most important fruit vegetables in various tropical countries. In okra, the Yellow Vein Mosaic Disease (YVMD) is caused by more than ten different viruses and the widespread incidence of disease transmitted by the vector Bemisia tabaci (Genn.) has highly affected the successful cultivation and production of the crop. Mutation breeding using radiations is one of the promising methods to get disease resistant elite cultivars of various crops. In this study, attempts were made to evaluate the genetic variability of gamma-ray irradiated mutant of okra. Four ISSR primers generated 26 amplified fragments. Of the 26 fragments, the lowest number of fragments (5) was produced by the primer ISSR12. Between the parent (P) and mutant (M4; mutant from fourth-generation) varieties of okra, primer ISSR07, primer ISSR16 and primer ISSR21 produced two monomorphic bands and three polymorphic bands (60% polymorphism) whereas primer ISSR12 produced one monomorphic band and three polymorphic bands (75% polymorphism). The mean percentage of polymorphism generated by four primers was 63.75%. Although the level of polymorphism exhibited by different primers varied from intermediate (60%) to high (75%), all primers could distinguish parent and gamma-irradiated mutant varieties of okra.

scholarly journals Genotype-by-Sequencing Analysis of Mutations and Recombination in Pepper Progeny of Gamma-Irradiated Gametophytes

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 144
Author(s):  
Yeong Deuk Jo ◽  
Han Sol Kang ◽  
Hong-Il Choi ◽  
Jin-Baek Kim
Keyword(s):  
Reverse Genetics ◽  
Mutation Screening ◽  
Mutation Breeding ◽  
Hot Pepper ◽  
Sequencing Analysis ◽  
Single Base ◽  
Base Substitutions ◽  
A Genome ◽  
Genotype By Sequencing ◽  
Gamma Irradiated

The irradiation of dry seeds is the most widely-used irradiation method for improving seed-propagated crops; however, the irradiation of other tissues also has useful effects. The irradiation of plant reproductive organs, rather than seeds, for mutation breeding has advantages, such as producing non-chimeric progeny. However, the mutation frequency and spectrum produced using this method have not been analyzed on a genome-wide level. We performed a genotype-by-sequencing analysis to determine the frequencies of single-base substitutions and small (1–2 bp) insertions and deletions in hot pepper (Capsicum annuum L.) plants derived from crosses using gamma-irradiated female or male gametophytes. The progeny of irradiated gametophytes showed similar or higher DNA mutation frequencies, which were dependent on the irradiation dose and irradiated tissue, and less biased single base substitutions than progeny of irradiated seeds. These characteristics were expected to be beneficial for development of mutation population with a high frequency of small DNA mutations and performing reverse-genetics-based mutation screening. We also examined the possible use of this irradiation method in manipulating the meiotic recombination frequency; however, no statistically significant increase was detected. Our results provide useful information for further research and breeding using irradiated gametophytes.

Employing Gamma Ray Irradiated Giardia lamblia as Trialed Vaccine in Experimental Animal

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Amal Kamil Abdul Sada ◽  
Amany Mohamed Al-Kaysi
Keyword(s):  
Giardia Lamblia ◽  
Gamma Ray ◽  
Age Groups ◽  
Active Phase ◽  
Study Groups ◽  
Negative Control ◽  
Control Group ◽  
High Dose ◽  
Histopathological Changes ◽  
Gamma Irradiated

This is an experimental trial to prepare a vaccine from gamma-irradiated Giardia lamblia which is evaluated in experimental animals. The study was conducted from December 2015 to April 2016. The field survey of the parasite was conducted from those patients attending the laboratories of the Alawi Children's Hospital in Rusafa and the Al-Yarmouk Teaching Hospital in Karkh, through which 1250 stool samples of different age groups were examined. Five groups of mice were used in the study; the first was injected with normal saline and considered as a negative control group, the second was injected with cystic form of non-irradiated Giardia lamblia and considered as a positive control group, whereas the other three groups were injected with gamma irradiated Giardia lamblia at three different doses 10, 15 and 25 rad respectively. Giardia lamblia was primarily cultivated in liver infusion agar for ten days to obtain the active phase. On the sixth day, the cystic phase was purified and standardized to be used in the infection of mice with or without the exposure of gamma rays. Mice showed high sensitivity to parasitic infestation, in the gamma non-irradiated and the irradiated with gamma 10 rad, and 15 rad irradiated groups which was 100%. The results expressed an excystation process of the depleted phases and the release of the feeder phases. The results of the three irradiated groups consisted of histopathological changes of the small, and the rectum by dissection after two weeks of infection, with intestine amputation lesions, as well as ulceration and inflammation of the inflammatory cells represented in small numbers of neutrophil, lymphocytes, and eosinophils. The presence of ulceration and fall of epithelial cells in the intestinal cavity has been shown, and different forms of the parasite have been observed. Mice which was injected with irradiated G lamblia at high dose (25 rad), not show and sensitivity to the challenge infection and no excystation of thy parasite had been done. After 2 wreaks, a comparison was achieved between all study groups in which no histopathological changes were noticed in the mice irradiated with dose of25 rad. After another two weeks, a challenge dose was given (un-attenuated G lamblia) and mice were dissected after another two weeks, no changes on the level of histopathology of intestinal tissue were noticed the results suggested that mice acquire an immunity against the parasite infection.

scholarly journals Arabidopsis Genes Essential for Seedling Viability: Isolation of Insertional Mutants and Molecular Cloning

Genetics ◽  
2001 ◽  
Vol 159 (4) ◽  
pp. 1765-1778
Author(s):  
Gregory J Budziszewski ◽  
Sharon Potter Lewis ◽  
Lyn Wegrich Glover ◽  
Jennifer Reineke ◽  
Gary Jones ◽  
...  
Keyword(s):  
Large Scale ◽  
Protein Translocation ◽  
Gene Families ◽  
Mutant Phenotype ◽  
Lethal Mutant ◽  
A Genome ◽  
Genes Encoding ◽  
High Level ◽  
Mutant Lines ◽  
Genome Scale

Abstract We have undertaken a large-scale genetic screen to identify genes with a seedling-lethal mutant phenotype. From screening ~38,000 insertional mutant lines, we identified >500 seedling-lethal mutants, completed cosegregation analysis of the insertion and the lethal phenotype for >200 mutants, molecularly characterized 54 mutants, and provided a detailed description for 22 of them. Most of the seedling-lethal mutants seem to affect chloroplast function because they display altered pigmentation and affect genes encoding proteins predicted to have chloroplast localization. Although a high level of functional redundancy in Arabidopsis might be expected because 65% of genes are members of gene families, we found that 41% of the essential genes found in this study are members of Arabidopsis gene families. In addition, we isolated several interesting classes of mutants and genes. We found three mutants in the recently discovered nonmevalonate isoprenoid biosynthetic pathway and mutants disrupting genes similar to Tic40 and tatC, which are likely to be involved in chloroplast protein translocation. Finally, we directly compared T-DNA and Ac/Ds transposon mutagenesis methods in Arabidopsis on a genome scale. In each population, we found only about one-third of the insertion mutations cosegregated with a mutant phenotype.

scholarly journals Chemical and Biological Profiles of Dendrobium in Two Different Species, Their Hybrid, and Gamma-Irradiated Mutant Lines of the Hybrid Based on LC-QToF MS and Cytotoxicity Analysis

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1376
Author(s):  
Bomi Nam ◽  
Hyun-Jae Jang ◽  
Ah-Reum Han ◽  
Ye-Ram Kim ◽  
Chang-Hyun Jin ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Squamous Carcinoma ◽  
Ultra Performance Liquid Chromatography ◽  
Carcinoma Cells ◽  
Traditional Medicines ◽  
Flight Mass Spectrometry ◽  
Squamous Carcinoma Cells ◽  
Chemical Profiles ◽  
Gamma Irradiated ◽  
Mutant Lines

The Dendrobium species (Orchidaceae) has been cultivated as an ornamental plant as well as used in traditional medicines. In this study, the chemical profiles of Dendrobii Herba, used as herbal medicine, Dendrobium in two different species, their hybrid, and the gamma-irradiated mutant lines of the hybrid, were systematically investigated via ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QToF MS). Among the numerous peaks detected, 17 peaks were unambiguously identified. Gigantol (1), (1R,2R)-1,7-hydroxy-2,8-methoxy-2,3-dihydrophenanthrene-4(1H)-one (2), tristin (3), (−)-syringaresinol (4), lusianthridin (5), 2,7-dihydroxy-phenanthrene-1,4-dione (6), densiflorol B (7), denthyrsinin (8), moscatilin (9), lusianthridin dimer (10), batatasin III (11), ephemeranthol A (12), thunalbene (13), dehydroorchinol (14), dendrobine (15), shihunine (16), and 1,5,7-trimethoxy-2-phenanthrenol (17), were detected in Dendrobii Herba, while 1, 2, and 16 were detected in D. candidum, 1, 11, and 16 in D. nobile, and 1, 2, and 16 in the hybrid, D. nobile × candidum. The methanol extract taken of them was also examined for cytotoxicity against FaDu human hypopharynx squamous carcinoma cells, where Dendrobii Herba showed the greatest cytotoxicity. In the untargeted metabolite analysis of 436 mutant lines of the hybrid, using UPLC-QToF MS and cytotoxicity measurements combined with multivariate analysis, two tentative flavonoids (M1 and M2) were evaluated as key markers among the analyzed metabolites, contributing to the distinction between active and inactive mutant lines.

scholarly journals In Vitro Mutagenesis of Etlingera elatior by Gamma Ray Intermittent Irradiation

2018 ◽  
Vol 5 (3) ◽  
pp. 111-118 ◽  
Author(s):  
Elmi Irmayanti Azzahra ◽  
Syarifah Iis Aisyah ◽  
Diny Dinarti ◽  
Krisantini Krisantini
Keyword(s):  
Gamma Ray ◽  
Morphological Changes ◽  
Leaf Shape ◽  
Morphological Diversity ◽  
Mutation Breeding ◽  
Ornamental Plant ◽  
In Vitro Mutagenesis ◽  
Split Dose ◽  
Etlingera Elatior

Etlingera elatior is tropical ornamental plant commonly called “torch ginger” from Zingiberaceae family. Conventional breeding of E. elatior is limited by cross incompatibility, poor fruit set and low seed production.  In this study irradiation of E. elatior with Gamma ray performed to induce mutation. This study was aimed to increase morphological diversity and to obtain unique morphological characters to increase the aesthetic value of E. elatior as ornamental plants and cut flower. Two genotypes of E. elatior, red and white flowers, were tested. The LD20, LD35, and LD50 were determined following intermittent (split dose) Gamma irradiation with a two-hour gap between each gamma ray shot. Red genotype E. elatior explants were irradiated with dose of 3 + 3 Gy (LD20); 4 + 4 Gy (LD35) and 5 + 5 Gy (LD50); white genotype were irradiated with a dose of 2 + 2 Gy (LD20); 2.8 + 2.8 Gy (LD35) and 3.7 + 3.7 Gy (LD50). Non-irradiated explants were set as control.  The results of this study indicated that the increase in dose of gamma ray irradiation changed shoot length and number of leaves in the red genotype per explant as compared to control. Morphological changes occurred in leaf shape at 5 + 5 Gy and 3.7 + 3.7 Gy and formation of variegated leaves at 2.8 + 2.8 Gy and 5 + 5 Gy. Keywords: mutation breeding, ornamental plant, split irradiation dose, torch ginger, Zingiberaceae

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