scholarly journals Preserving Biodiversity as Source of Health Promoting Compounds: Phenolic Profile and Biological Activity of Four Varieties of Solanum lycopersicum L.

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 447
Author(s):  
Immacolata Faraone ◽  
Daniela Russo ◽  
Maria Ponticelli ◽  
Vincenzo Candido ◽  
Donato Castronuovo ◽  
...  
Keyword(s):  
Biological Activity ◽  
Solanum Lycopersicum ◽  
Radical Scavenging ◽  
Carotenoid Content ◽  
Solanum Lycopersicum L ◽  
Dpph Method ◽  
Wide Range ◽  
Health Promoting ◽  
Phytochemical Profile ◽  
Peel Extract

Tomato (Solanum lycopersicum L.) is a precious source of specialized metabolites with a great role in human health. Many varieties of tomatoes characterize the south of Italy’s agronomic production and biodiversity, thanks to its warm temperatures and favorable weather. The preservation of biodiversity is a major goal of recent years, as each variety shows a peculiar phytochemical profile and provides a wide variety of metabolites with health-beneficial properties. Among the wide range of tomato varieties, literature focused on the most commercially-known types, including San Marzano and Datterino, while this study considered typical South Italy varieties for the first time, as well as Crovarese and Arsicolo. The aim of our work is to enrich the current knowledge about the tomato by evaluating the carotenoid content, the phytochemical profile by HPLC-DAD, and the biological activity of the different parts (peel, fruit, pulp, and seeds) of niche cultivars compared with commercial ones. Radical scavenging activity, assessed by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method, was higher in Crovarese peel extract, while Arsicolo possessed the highest lycopene content, underlying the importance of local ecotypes as a precious source of health promoting compounds. However, out of all of the varieties considered, peel extract was the most active one, opening new insights on their valorization in light of the circular economy.

Evaluation of antioxidant enzyme content, phenolic content, and antibacterial activity of Commiphora gileadensis grown in Saudi Arabia

2021 ◽  
Vol 19 (4) ◽  
pp. 329-343
Author(s):  
Yaaser Q. Almulaiky ◽  
Ammar AL-Farga
Keyword(s):  
Antibacterial Activity ◽  
Saudi Arabia ◽  
Oral Hygiene ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Reference Drug ◽  
Wide Range ◽  
Peel Extract

Commiphora gileadensis is commonly used in Saudi Arabia for oral hygiene. A lack of data about its biological activity encouraged us to evaluate the antioxidant and antibacterial activities of its leaf and stem extracts. Ethanol, methanol, acetone and deionized water were tested as extraction solvents. 80% methanol gave the highest extracted concentrations of phenolic and flavonoid substances. The leaf and stem extracts were respectively evaluated for their radical scavenging activity with DPPH (EC50 = 3.39, and 1.06), ABTS (EC50 = 0.690, and 0.55), and peroxide scavenging activity (EC50 = 2.43, and 1.28). GC-MS identified a wide range of compounds that may be responsible for these activities of the results observed. The highest levels of chlorophyll, carotenoids, and lycopene were found in the leaf extract while level of proanthocyanidins was found in the stem peels extract. The peroxidase and catalase activities of stem peel extract were higher than those of the leaf extract. The findings showed that the leaf and stem peel extracts of C. gileadensis exhibited significant antibacterial activity against the test organisms. The minimum inhibitory concentrations for the plant extracts were compared with the standard reference drug Augmentin but the time–kill curves for the C. gileadensis extracts showed that they were less effective than Augmentin. Moreover, the stem peel extract exhibited stronger antibacterial activity than the leaf extract. In conclusion, C. gileadensis can be an important source of natural antioxidants, used as a healthy chewing stick for teeth brushing and oral hygiene purposes.

scholarly journals Low Dose of Fluoride in the Culture Medium of Cordyceps militaris Promotes Its Growth and Enhances Bioactives with Antioxidant and Anticancer Properties

2021 ◽  
Vol 7 (5) ◽  
pp. 342
Author(s):  
Xiaoshuai Li ◽  
Jia Wang ◽  
Huayue Zhang ◽  
Long Xiao ◽  
Zhongfang Lei ◽  
...  
Keyword(s):  
Low Dose ◽  
Radical Scavenging ◽  
Potassium Fluoride ◽  
Cordyceps Militaris ◽  
Carotenoid Content ◽  
Market Demand ◽  
Fruiting Bodies ◽  
Human Osteosarcoma Cells ◽  
Wide Range ◽  
Bioactive Ingredients

Cordyceps militaris possesses several compounds with medicinal properties, and is commonly used in traditional Chinese functional food and medicine for a variety of health benefits. Because of its rare occurrence in nature, the market demand for artificial C. militaris is on the rise. Furthermore, efforts to increase its bioactive ingredients have also been considered in research. In this study, we aimed to investigate the effect of fluoride on the growth and enrichment of bioactive compounds in C. militaris. A wide range of potassium fluoride concentrations (0, 0.001, 0.01, 0.1, and 1 mM) were added to the culture media as a source of fluoride during the cultivation of C. militaris fruiting bodies. The contents of fluorine and bioactive substances of the fruiting bodies in normal (NM) and fluorine-supplemented (FM) media were measured and compared. C. militaris raised in the growth medium supplemented with 0.01 mM potassium fluoride led to a 44.86% (1.55 ± 0.14 g/bottle) increase in biomass and a 23.43% (3161.38 ± 35.71 µg/g) increase in total carotenoid content in the fruiting bodies. Furthermore, a remarkable increase in superoxide dismutase-like activity (84.75 U/mg) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (IC50 = 2.59 mg/mL) was recorded. In human cancer cell-based assays, C. militaris raised in FM caused stronger cytotoxicity, apoptosis, and cell cycle arrest in human osteosarcoma cells. These results demonstrated that a low dose of fluoride could stimulate the growth of C. militaris fruiting bodies and enhance the production of bioactive ingredients that possess useful antioxidant and anticancer activities.

scholarly journals Genetic variability and trait relationship in cherry tomato (Solanum lycopersicum L. var. cerasiforme (Dunnal) A. Gray)

2013 ◽  
Vol 41 (2) ◽  
pp. 163-167 ◽  
Author(s):  
MS Islam ◽  
HC Mohanta ◽  
MR Ismail ◽  
MY Rafii ◽  
MA Malek
Keyword(s):  
Genetic Variability ◽  
Solanum Lycopersicum ◽  
Fruit Weight ◽  
Fruit Yield ◽  
High Yield ◽  
Genetic Advance ◽  
Cherry Tomato ◽  
Solanum Lycopersicum L ◽  
Wide Range ◽  
Additive Gene

Nine traits of 11 cherry tomato (Solanum lycopersicum L.) var. cerasiforme (Dunal) A. Gray) inbred lines exhibited a wide range of genetic variability. High geno- and phenotypic coefficients of variation were obtained for individual fruit weight (68.16 and 74.23%, respectively) followed by number of fruits/plant (58.8 and 68.34%, respectively). High estimates of heritability, genetic advance and genotypic coefficient of variation for the traits of individual fruit weight, number of fruits and clusters/plant were controlled by additive gene action indicating the possibility of selection to improve these characters. Fruit yield/plant showed low heritability along with low genetic advance and did not show significant and positive correlation with the remaining characters. It indicates that improvement of high yield through selection is difficult, rather hybridization can be effective for improving the fruit yield/plant. Among the lines, CH154 produced the highest number of fruits/plant (291) and highest fruit yield (1.89 kg/plant and 63.4 t/ha) and can be selected for cultivation under Bangladesh condition. DOI: http://dx.doi.org/10.3329/bjb.v41i2.13443 Bangladesh J. Bot. 41(2): 163-167, 2012 (December)

scholarly journals Study of Genetic Variability and Heritability in Cherry Tomato (Solanum lycopersicum L. Var. cerasiforme) Genotypes

2021 ◽  
pp. 76-81
Author(s):  
Afroza Akhter ◽  
Ambreen Nabi ◽  
Baseerat Afroza ◽  
Zahoor Ahmad Dar ◽  
Ajaz Ahmad Malik ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Solanum Lycopersicum ◽  
Coefficient Of Variation ◽  
Fruit Weight ◽  
Cherry Tomato ◽  
Total Carotenoids ◽  
Solanum Lycopersicum L ◽  
Wide Range ◽  
Almost All ◽  
Genotypic Coefficient Of Variation

Twenty-nine genotypes of cherry tomato (Solanum lycopersicum L. var. cerasiforme) were evaluated for their genetic variability and heritability. A wide range of genetic variability was observed for twenty traits of cherry tomato genotypes. The analysis of variance was significant for all the characters indicating genetic variability in the genotypes under the study except number of locules per fruit. The highest phenotypic and genotypic coefficient of variation were observed for lycopene (43.12, 42.94), total carotenoids (42.82, 42.51), average fruit weight (42.52, 42.50), fruit yield per hectare (42.29, 42.24). In general Phenotypic Coefficient of Variation (PCV) was marginally higher than the corresponding Genotypic Coefficient of Variation (GCV) indicating the less influence of environment in the expression of traits under study. High heritability coupled with high genetic advance as percentage of mean was observed for almost all characters indicating that most of the traits studied were mainly controlled by additive gene effect and thus selection may be effective.

Near-isogenic lines enhancing ascorbic acid, anthocyanin and carotenoid content in tomato (Solanum lycopersicum L. cv Micro-Tom) as a tool to produce nutrient-rich fruits

2014 ◽  
Vol 175 ◽  
pp. 111-120 ◽  
Author(s):  
Ivan Sestari ◽  
Agustín Zsögön ◽  
Gabriel Garcia Rehder ◽  
Luciane de Lira Teixeira ◽  
Neuza Mariko Aymoto Hassimotto ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Solanum Lycopersicum ◽  
Carotenoid Content ◽  
Near Isogenic Lines ◽  
Isogenic Lines ◽  
Solanum Lycopersicum L

scholarly journals PHYTOCHEMICAL CONTENT AND ANTIOXIDANT ACTIVITIES IN DIFFERENT ORGANS OF POMELO (CITRUS MAXIMA [BURM.] MERR.) USING 2,2-DIPHENYL-1-PICRYLHYDRAZYL AND PHOSPHOMOLYBDENUM ASSAYS

2016 ◽  
pp. 185 ◽  
Author(s):  
Irda Fidrianny ◽  
Elvira Sari ◽  
Komar Ruslan
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Phytochemical Content ◽  
Dpph Method ◽  
Citrus Maxima ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Peel Extract

<p>ABSTRACT<br />Objectives: The aims of this research were to determine antioxidant activity from various organs extracts of pomelo using 2,2-diphenyl-1picrylhydrazyl<br />(DPPH)<br />and<br />phosphomolybdenum<br />assays,<br />total<br />phenolic,<br />flavonoid,<br />and<br />carotenoid<br />content,<br />correlation<br />of<br />total<br />phenolic,<br />flavonoid,</p><p>and<br />carotenoid<br />content<br />in<br />various<br />extracts<br />of<br />chayote<br />with their<br />inhibitory<br />concentration<br />50%<br />(IC<br />) of DPPH antioxidant activities and exhibitory<br />concentration 50% (EC<br />50<br />) of phosphomolybdenum capacity, and correlation between two antioxidant assays.<br />50<br />Methods: Extraction was carried out by reflux using various polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant<br />activities using DPPH and phosphomolybdenum assays, determination of total phenolic, flavonoid, and carotenoid content were conducted by UVvisible<br />spectrophotometry<br />and its correlation<br />with IC<br />Results: The lowest IC<br />50<br />50<br /> of DPPH and EC<br />50<br /> of phosphomolybdenum was analyzed by Pearson’s method.<br /> of DPPH scavenging activity was shown by ethyl acetate cortex extract of pomelo (0.68 µg/ml), whereas the lowest EC<br /> of<br />phosphomolybdenum capacity was given by ethyl acetate leaves extract of pomelo (101.36 µg/ml). Ethyl acetate cortex extract of pomelo had the<br />highest total phenolic content and ethyl acetate leaves extract had the highest total flavonoid content (TFC). There was a negative and significant<br />correlation between TFC in cortex and peel extracts of pomelo with their IC<br />50<br /> of DPPH.<br />Conclusions: All organs extracts of pomelo (except n-hexane peel extract) were classified as a very strong antioxidant by DPPH method. Flavonoid<br />compounds in cortex and peel extract of pomelo were the major contributor in antioxidant activities by DPPH method. DPPH and phosphomolybdenum<br />assays gave no linear results in antioxidant activities of leaves, cortex, and peel extracts of pomelo.<br />Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Phosphomolybdenum, Pomelo, Three organs.<br />50</p>

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