Hypolipidemic and Antioxidant Effects of Guishe Extract from Agave lechuguilla, a Mexican Plant with Biotechnological Potential, on Streptozotocin-Induced Diabetic Male Rats

In the present study, we used a by-product from Agave lechuguilla (guishe) to test its antidiabetic effect, hypolipidemic activity, and capacity to mitigate the oxidative stress in kidney mitochondria from streptozotocin-induced diabetic rats. Orally, a crude aqueous extract from lyophilized guishe was administered over 5 weeks at different doses. Blood glucose and body weight were monitored. Also, blood chemistry, bilirubin, and alanine aminotransferase were assayed. Furthermore, the activity of catalase, thiobarbituric acid reactive species, mitochondrial superoxide dismutase, glutathione and glutathione peroxidase were determined in isolated kidney mitochondria. Our results show that guishe extracts have no antidiabetic properties at any dose. Nevertheless, it was able to diminish serum triglyceride levels and regulate the oxidative stress observed in isolated kidney mitochondria. These observations indicate that the aqueous extract from guishe can be used to treat abnormalities in serum lipids, as a hypolipidemic, and mitigate the oxidative stress, as an antioxidant, occurring during diabetes.

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Cardioprotective effects of Fenugreek (Trigonella foenum-graceum) seed extract in streptozotocin induced diabetic rats

Journal of Cardiovascular and Thoracic Research ◽

10.34172/jcvtr.2021.01 ◽

2021 ◽

Vol 13 (1) ◽

pp. 28-36

Author(s):

Soleyman Bafadam ◽

Maryam Mahmoudabady ◽

Saeed Niazmand ◽

Seyed Abdolrahim Rezaee ◽

Mohammad Soukhtanloo

Keyword(s):

Oxidative Stress ◽

Thiobarbituric Acid ◽

Serum Glucose ◽

Diabetic Rats ◽

Seed Extract ◽

Male Rats ◽

Therapeutic Effects ◽

Sod Activity ◽

Fenugreek Seed ◽

Cardioprotective Effects

Introduction: Inadequate control of diabetes mellitus (DM) leads to considerable cardiovascular implications like diabetic cardiomyopathy (DCM). Cardiomyocyte apoptosis is one of the main mechanisms of DCM pathogenesis associated with hyperglycemia, oxidative stress, inflammation, hyperlipidemia and several other factors. Trigonella foenum-graecum (Fenugreek) has been long used as a traditional medicine and has many therapeutic effects, including anti-diabetic, anti-hyperlipidemia, anti-inflammatory and anti-oxidant properties. The current study aimed to investigate cardioprotective effects of fenugreek seed on diabetic rats. Methods: Diabetes was induced in forty-two male rats by injection of streptozotocin (STZ) (60 mg/ kg). Diabetic animals were treated with three different doses of fenugreek seed extract (50, 100 and 200 mg/kg) or metformin (300 mg/kg) for six weeks by gavage. Nondiabetic rats served as controls. Glucose, cholesterol, and triglycerides levels were measured in the blood samples, and oxidative stress markers as well as gene expression of ICAM1, Bax and Bcl2 were assessed in the cardiac tissues of the experimental groups. Results: Diabetic rats exhibited increased serum glucose, cholesterol and triglycerides levels, elevated markers of oxidative stress thiobarbituric acid–reacting substances (TBARS) levels , total thiol groups (SH), catalase (CAT) and superoxide dismutase (SOD) activity, and enhanced apoptosis cell death (ratio of Bax/Bcl2). Fenugreek seed extract considerably improved metabolism abnormalities, attenuated oxidative stress and diminished apoptosis index. Conclusion: Our study suggests that fenugreek seed may protect the cardiac structure in STZ-induced diabetic rats by attenuating oxidative stress and apoptosis.

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Attenuation of oxidative stress in hepatic and pancreatic tissues of STZ-induced diabetic rats treated with aqueous extract of Vochysia rufa

Planta Medica ◽

10.1055/s-0034-1394539 ◽

2014 ◽

Vol 80 (16) ◽

Cited By ~ 2

Author(s):

NM De Gouveia ◽

IB Moraes ◽

RMF Sousa ◽

MB Neto ◽

AV Mundim ◽

...

Keyword(s):

Oxidative Stress ◽

Aqueous Extract ◽

Diabetic Rats

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Study the Effect of Gurmmar Aqueous Extract on Oxidative Stress of Diabetic Rats

Egyptian Journal of Nutrition ◽

10.21608/enj.2018.142708 ◽

2018 ◽

Vol 33 (1) ◽

pp. 1-35

Keyword(s):

Oxidative Stress ◽

Aqueous Extract ◽

Diabetic Rats

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Zygophyllum album aqueous extract reduces glycemia and ameliorates lipid profile and oxidative damage in hypercholesterolemic-diabetic rats

Nutrition & Food Science ◽

10.1108/nfs-03-2018-0078 ◽

2019 ◽

Vol 49 (1) ◽

pp. 141-157 ◽

Cited By ~ 3

Author(s):

Yasmina Bahlil ◽

Djamil Krouf ◽

Nawal Taleb-Dida

Keyword(s):

Adipose Tissue ◽

Oxidative Damage ◽

Aqueous Extract ◽

Thiobarbituric Acid ◽

Diabetic Rats ◽

Paraoxonase 1 ◽

Thiobarbituric Acid Reactive Substance ◽

Model Assessment ◽

Cholesterol Acyl Transferase ◽

Content Type

Purpose This paper aims to study the effects of Zygophyllum album (Za) (Z. album) in hypercholesterolemic-diabetic rats. Design/methodology/approach Male Wistar rats (n = 36) weighing 200 ± 10 g, consumed an experimental diet containing 20 per cent casein were divided into three groups (n = 12). The first group consumed the diet enriched with 1 per cent cholesterol (CH), the second group is rendered diabetic by intraperitoneal injection of streptozotocin (STZ) (35 mg/kg body weight) (D) (DM). The third group is hypercholesterolemic and STZ-induced diabetic (CH-DM); each group was subdivided into two groups (n = 6), non-treated groups (CH, DM and CH-DM) and treated groups supplemented with 1 per cent Z. album lyophilized aqueous extract (CH-Z, DM-Z and CH-DM-Z). Findings In CH-DM group, Z. album decreases glycemia (−15 per cent) and inversely increases insulinemia (+28 per cent) and Homeostasis Model Assessment of Insulin Resistance (+19 per cent). In liver, total cholesterol (TC) and triacyglycerols (TAG) levels were reduced by −57 per cent and −29 per cent, respectively. In plasma, TC concentration was increased by +20 per cent, whereas those of TAG level were lowered by −56 per cent. Lecithin cholesterol acyl transferase and paraoxonase 1 activities were raised by +45 and +59 per cent, respectively. Inversely, thiobarbituric acid reactive substance levels were lowered significantly in liver, heart, kidney and adipose tissue (p < 0.05). Superoxide dismutase activity was enhanced in liver (+54 per cent), heart (+36 per cent), kidney (+45 per cent) and adipose tissue (59 per cent). Liver glutathione peroxidase (GSH-Px) activity was enhanced by +38 per cent and heart activities of GSH-Px, and glutathione reductase (GSSH-Red) were increased by +25 and +18 per cent. In kidney, GSH-Px activity was reduced by −26 per cent; in reverse, GSSH-Red activity was increased by +14 per cent. In adipose tissue, GSH-Px and GSSH-Red activities were augmented by +35 and +15 per cent. Originality/value These results suggest that Z. album aqueous extract has antihyperglycemic and antihyperlipemic actions. Also, Z. album protects against tissue oxidative damage; therefore, it can help to prevent cardiovascular complications of diabetes combined with hypercholesterolemia.

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Fortified Extract of Red Berry,Ginkgo biloba, and White Willow Bark in Experimental Early Diabetic Retinopathy

Journal of Diabetes Research ◽

10.1155/2013/432695 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 20

Author(s):

Claudio Bucolo ◽

Giuseppina Marrazzo ◽

Chiara Bianca Maria Platania ◽

Filippo Drago ◽

Gian Marco Leggio ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Diabetic Retinopathy ◽

Ginkgo Biloba ◽

Plasma Lipid ◽

Thiobarbituric Acid ◽

Diabetic Rats ◽

Sprague Dawley ◽

White Willow ◽

Willow Bark

Diabetic retinopathy is a complex condition where inflammation and oxidative stress represent crucial pathways in the pathogenesis of the disease. Aim of the study was to investigate the effects of a fortified extract of red berries,Ginkgo bilobaand white willow bark containing carnosine andα-lipoic acid in early retinal and plasma changes of streptozotocin-induced diabetic rats. Diabetes was induced by a single streptozotocin injection in Sprague Dawley rats. Diabetics and nondiabetic (control) rats were treated daily with the fortified extract for the ten days. Retina samples were collected and analyzed for their TNF-αand VEGF content. Moreover, plasma oxidative stress was evaluated by thiobarbituric acid reacting substances (TBARS). Increased TNF-αand VEGF levels were observed in the retina of diabetic rats. Treatment with the fortified extract significantly lowered retinal cytokine levels and suppressed diabetes-related lipid peroxidation. These data demonstrate that the fortified extract attenuates the degree of retinal inflammation and plasma lipid peroxidation preserving the retina in early diabetic rats.

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Spirulina maxima and its effect on antioxidant activity in fructose induced oxidative stress with histopathological observations

Acta Facultatis Pharmaceuticae Universitatis Comenianae ◽

10.1515/afpuc-2015-0027 ◽

2015 ◽

Vol 62 (2) ◽

pp. 13-19

Author(s):

Urmila Jarouliya ◽

Anish Zacharia ◽

Raj K. Keservani ◽

Godavarthi B.K.S Prasad

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Superoxide Dismutase ◽

Blood Glucose ◽

Reduced Glutathione ◽

Thiobarbituric Acid ◽

Diabetic Rats ◽

Therapeutic Effects ◽

Thiobarbituric Acid Reactive Substances ◽

Spirulina Maxima

Abstract Diabetes mellitus is a metabolic disorder characterised by hyperglycemia and oxidative stress. The aim of the present study is to explore the antioxidant effect of Spirulina maxima in rat model along with the histopathological observations. Diabetes was induced by feeding 10% fructose solution orally to Wistar rats (n = 6) for 30 days, analysed for plasma blood glucose and the markers of the oxidative stress [catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS)]. These biochemical studies were associated with histopathological examination of liver and kidney sections. The microalga Spirulina maxima being rich in proteins and other essential nutrients is widely used as a food supplement. S. maxima at a dose of 5 and 10% per kg and the metformin (500 mg/kg) as reference drug were given orally for 30 days to the diabetic rats. Diabetic rats showed significant (p < 0.001) elevations in plasma blood glucose, thiobarbituric acid-reactive substances and significant reduction in catalase, superoxide dismutase and reduced glutathione activity. Oral administration of 5 and 10% aqueous extract of S. maxima for 30 days restored not only of blood glucose levels but also markers of oxidative stress. Histopathological observations of tissues manifested that the S. maxima administration had the protective and therapeutic effects against fructose-induced abnormalities in diabetic rats. It is concluded that S. maxima is effective in reinstating the antioxidant activity in addition to its antidiabetic effect in type 2 diabetic rats.

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Gentiopicroside attenuates diabetic retinopathy by inhibiting inflammation, oxidative stress, and NF-κB activation in rat model

European Journal of Inflammation ◽

10.1177/2058739219847837 ◽

2019 ◽

Vol 17 ◽

pp. 205873921984783 ◽

Cited By ~ 2

Author(s):

Xian Zhang ◽

En Shi ◽

Lan Yang ◽

Weina Fu ◽

Feng Hu ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetic Retinopathy ◽

Mtt Assay ◽

Interleukin 1 ◽

Phosphate Buffer Solution ◽

Diabetic Rats ◽

Angiogenic Factors ◽

Male Rats ◽

Buffer Solution ◽

Defensive Role

Diabetic retinopathy, an inflammatory condition, is one of the devastating complication associated with diabetes that can lead to irreversible blindness. Gentiopicroside (GP), a secoiridoid glycoside, exhibits anti-inflammatory and antioxidant activity. The investigation was carried out to explore whether GP could attenuate diabetic retinopathy in diabetic rats. Diabetes was induced by injecting streptozotocin (STZ) (65 mg/kg) intraperitoneally in 8-weeks-old male rats (200–240 g). The treatment group received GP (20, 40, 80 mg/kg) orally for a duration of 10 weeks in diabetic rats (n = 10), and the diabetic group animals received phosphate buffer solution (n = 20). Effect of GP on cell viability study was performed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Oxidative stress markers, inflammatory mediators, and angiogenic factors were quantified in the retinal tissues of diabetic animals. All data were analyzed by one-way analysis of variance (ANOVA) at P < 0.05. Cytoprotective effect of GP was observed in MTT assay. GP effectively downregulated inflammatory cytokine, nuclear factor κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin 1 beta (IL-1β), and intercellular adhesion molecules-1 (ICAM-1), and upregulated antioxidant markers glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) in the retina of diabetic rats. GP equilibrated the disturbed angiogenic factors in the diabetic retinal tissues. Results clearly indicated defensive role of GP in the treatment of diabetic retinopathy by inhibition of NF-κB and oxidative stress.

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